Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,238,303 G→C 8.2% R483R (CGG→CGC uvrB → uvrABC excinuclease protein B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,238,3030GC 8.2% 555.4 / 29.8 171R483R (CGG→CGCuvrBuvrABC excinuclease protein B
Reads supporting (aligned to +/- strand):  ref base G (70/87);  new base C (8/6);  total (78/93)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 172 total aligned reads displayed.

TGTCCGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGTTCCGAGCGCTCC  >  NC_002947/2238155‑2238450
                                                                                                                                                    |                                                                                                                                                    
    cGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGga                                                                                                                                                 <  1:1048921/150‑1 (MQ=255)
    cGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGga                                                                                                                                                 <  2:3294336/150‑1 (MQ=255)
    cGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGga                                                                                                                                                 >  1:2271155/1‑150 (MQ=255)
    cGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGga                                                                                                                                                 <  2:45928/150‑1 (MQ=255)
    cGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGga                                                                                                                                                 <  2:1434721/150‑1 (MQ=255)
    cGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGga                                                                                                                                                 >  1:1317607/1‑150 (MQ=255)
      agaTCCGCACGCGTGGCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTGGACCAACCGCAGGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGgaga                                                                                                                                               <  1:2196177/150‑1 (MQ=255)
      agaTCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGgaga                                                                                                                                               >  2:1387361/1‑150 (MQ=255)
       gaTCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGAt                                                                                                                                              <  1:3512189/150‑1 (MQ=255)
        aTCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGAtt                                                                                                                                             >  1:1188305/1‑150 (MQ=255)
            gCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATcc                                                                                                                                         <  1:2603031/150‑1 (MQ=255)
              aaGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCcgc                                                                                                                                       <  1:4990364/150‑1 (MQ=255)
                 cGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCTAACGCCTGGCCGCGGACCTCTGCGTTTCCCTGGCCGACCACGCCGTGCGGGTAGGCGACCTGCCCTCGGACATCGACCCGGTGGAGCGGGTGGAGATTATCCGCGAc                                                                                                                                    <  1:3507264/150‑1 (MQ=255)
                    gtCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTg                                                                                                                                 >  1:1835908/1‑150 (MQ=255)
                     tCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTgc                                                                                                                                <  2:222042/150‑1 (MQ=255)
                     tCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTgc                                                                                                                                <  2:222386/150‑1 (MQ=255)
                      cgcgGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTgcg                                                                                                                               >  2:5406487/1‑150 (MQ=255)
                           cAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTgg                                                                                                                          <  1:5200006/150‑1 (MQ=255)
                             ggCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGc                                                                                                                        <  1:60166/150‑1 (MQ=255)
                             ggCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGc                                                                                                                        >  1:3782419/1‑150 (MQ=255)
                             ggCGAGCGAGTGCTGGCGACCACGATGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGc                                                                                                                        >  1:3782827/1‑150 (MQ=255)
                               cgagcgagTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATcc                                                                                                                                         <  1:1002289/131‑1 (MQ=255)
                               cgagcgagTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATcc                                                                                                                                         >  2:1002289/1‑131 (MQ=255)
                                gagcgagTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCAcc                                                                                                                     >  2:2068482/1‑150 (MQ=255)
                                gagcgagTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCAcc                                                                                                                     <  2:1317607/150‑1 (MQ=255)
                                       tGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACg                                                                                                              >  2:3742273/1‑150 (MQ=255)
                                         cTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTg                                                                                                            >  2:335794/1‑150 (MQ=255)
                                              gACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGgaga                                                                                                                                               <  1:4559942/110‑1 (MQ=255)
                                              gACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCtggt                                                                                                       >  2:3212174/1‑150 (MQ=255)
                                              gACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCtggt                                                                                                       >  1:185208/1‑150 (MQ=255)
                                              gACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCtggt                                                                                                       >  2:3212114/1‑150 (MQ=255)
                                               aCCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCtggtg                                                                                                      <  2:4563598/150‑1 (MQ=255)
                                               aCCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCtggtg                                                                                                      <  1:3789825/150‑1 (MQ=255)
                                                ccACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCtggtgg                                                                                                     <  1:1392687/150‑1 (MQ=255)
                                                       gACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCaa                                                                                              >  1:1966215/1‑150 (MQ=255)
                                                          cAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACct                                                                                           >  1:1751242/1‑150 (MQ=255)
                                                           aaaCGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACctg                                                                                          >  1:2751686/1‑150 (MQ=255)
                                                           aaaCGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACctg                                                                                          >  2:1226507/1‑150 (MQ=255)
                                                             gcGCATGGCAGAAGACCTGACCGAATATCTGCATGAACAAGGTGTTCGCGTACGTTACATGCACTCGGACATCGACACGCTGGAACGCATCGAAATCATCCGCGATCTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACctgct                                                                                        <  1:1605969/149‑1 (MQ=255)
                                                             gcGCATGGCAGAAGACCTGACCGAATATCTGCATGAACAAGGTGTTCGCGTACGTTACATGCACTCGGACATCGACACGCTGGAACGCATCGAAATCATCCGCGATCTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACctgct                                                                                        <  1:1092431/149‑1 (MQ=255)
                                                             aCGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACctgct                                                                                        >  2:2672127/1‑150 (MQ=255)
                                                               gCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCtgctgc                                                                                      <  2:3859139/150‑1 (MQ=255)
                                                                   ggCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGa                                                                                  <  2:1423927/150‑1 (MQ=255)
                                                                   ggCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGa                                                                                  <  2:5108599/150‑1 (MQ=255)
                                                                         ggACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCt                                                                            <  2:120394/150‑1 (MQ=255)
                                                                         ggACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCt                                                                            <  1:3580637/150‑1 (MQ=255)
                                                                          gACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTg                                                                           <  2:454931/150‑1 (MQ=255)
                                                                          gACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCGGGTGGGCATCAACCTGCTGCGCGAGGGCCTg                                                                           >  2:4173079/1‑150 (MQ=255)
                                                                           aCCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTgg                                                                          <  1:3010164/150‑1 (MQ=255)
                                                                            ccTCAGCGATTTCCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGa                                                                         <  2:3080503/150‑1 (MQ=255)
                                                                            ccTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGa                                                                         <  2:1924194/150‑1 (MQ=255)
                                                                              tCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGa                                                                                  >  1:3697203/1‑139 (MQ=255)
                                                                                   gATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCt                                                                                                                            <  2:2390781/92‑1 (MQ=255)
                                                                                   gATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCt                                                                                                                            >  1:2390781/1‑92 (MQ=255)
                                                                                   gATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGccc                                                                  >  2:2991976/1‑150 (MQ=255)
                                                                                      tACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAg                                                               <  1:2797076/150‑1 (MQ=255)
                                                                                          tGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGgtgt                                                           >  1:328332/1‑150 (MQ=255)
                                                                                              cGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCt                                                      <  2:4686106/150‑1 (MQ=255)
                                                                                              cGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCt                                                      <  2:4686175/150‑1 (MQ=255)
                                                                                               gACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCtg                                                     >  1:1678431/1‑150 (MQ=255)
                                                                                                  cACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCtggtg                                                  >  1:905564/1‑150 (MQ=255)
                                                                                                   acgacgTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCtggtgg                                                 >  2:4441148/1‑150 (MQ=255)
                                                                                                     gacgTGCGGGTACGCTACCTGCACTCGGACATCGACTCGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTgg                                                                          <  1:2553565/124‑1 (MQ=255)
                                                                                                      acgTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACGTGCCCGAGGTGTCGCTGGTGGCCa                                              <  1:3996156/150‑1 (MQ=255)
                                                                                                        gTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATc                                            >  1:1380601/1‑150 (MQ=255)
                                                                                                         tGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATcc                                           <  2:3635188/150‑1 (MQ=255)
                                                                                                                 cGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCg                                   <  1:4959182/150‑1 (MQ=255)
                                                                                                                   cTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGa                                 >  2:1130851/1‑150 (MQ=255)
                                                                                                                     aCCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACa                               >  1:2155104/1‑150 (MQ=255)
                                                                                                                      ccTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACac                              >  2:3527577/1‑149 (MQ=255)
                                                                                                                      ccTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACaa                              <  1:2161499/150‑1 (MQ=255)
                                                                                                                       cTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACaag                             >  1:1136212/1‑150 (MQ=255)
                                                                                                                         gCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACaagga                           >  2:5344909/1‑150 (MQ=255)
                                                                                                                          cACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACaaggaa                          <  2:3370345/150‑1 (MQ=255)
                                                                                                                            cTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACaaggaagg                        >  2:4809546/1‑150 (MQ=255)
                                                                                                                             tCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGt                       <  2:2514902/150‑1 (MQ=255)
                                                                                                                             tCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTAGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGACATCCTCGATGCGGACAAGGAAGGt                       >  1:877021/1‑150 (MQ=255)
                                                                                                                             tCGGACATCGACACGCTGGAACGCATCGAAATCATCCGCGATCTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACCTGCTGCGCGAGGGTCTCGATATTCCGGAATGCGGTTTCGTCGCCATTCTGGATGCCGACaaggaaggc                       >  1:564959/1‑149 (MQ=255)
                                                                                                                             tCGGACATCGACACGCTGGAACGCATCGAAATCATCCGCGAACTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACCTGCTGCGCGAGGGTCTCGATATTCCGGAATGCGGTTTCGTCGCCATTCTGGATGCCGACaaggaaggc                       >  1:2172267/1‑149 (MQ=255)
                                                                                                                                 aCATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGACGGTTTTc                   >  2:4023470/1‑150 (MQ=255)
                                                                                                                                 aCATCGACACGCTGGAACGCATCGAAATCATCCGCGATCTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACCTGCTGCGCGAGGGTCTCGATATTCCGGAATGCGGTTTCGTCGCCATTCTGGATGCCGACAAGGAAGGcttcc                   <  2:1832817/150‑3 (MQ=255)
                                                                                                                                 aCATCGACACGCTGGAACGCATCGAAATCATCCGCGATCTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACCTGCTGCGCGAGGGTCTCGATATTCCGGAATGCGGTTTCGTCGCCATTCTGGATGCCGACAAGGAAGGcttcc                   <  2:1832861/150‑3 (MQ=255)
                                                                                                                                  cATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCt                  <  2:556028/150‑1 (MQ=255)
                                                                                                                                  cATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCt                  <  2:1836036/150‑1 (MQ=255)
                                                                                                                                  cATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCt                  <  2:273761/150‑1 (MQ=255)
                                                                                                                                   aTCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTg                 >  2:1740552/1‑150 (MQ=255)
                                                                                                                                   aTCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTg                 >  1:2647136/1‑150 (MQ=255)
                                                                                                                                     cGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCg               <  2:5280452/150‑1 (MQ=255)
                                                                                                                                      gACACGCTGGAACGCATCGAAATCATCCGCGATCTGCGCCTTGGCGCTTTCGATGTATTGGTGGGTATCAACCTGCTGCGCGAGGGTCTCGATATTCCGGAATGCGGTTTCGTCGCCATTCTGGATGCCGACAAGGAAGGCTTCCTACGt              <  1:4039396/150‑1 (MQ=255)
                                                                                                                                       acacGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGc                                                                                                   >  2:5235163/1‑65 (MQ=255)
                                                                                                                                       acacGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGc                                                                                                   <  1:5235163/65‑1 (MQ=255)
                                                                                                                                       acacGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGtt             >  1:55163/1‑150 (MQ=255)
                                                                                                                                       acacGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGtt             >  1:77871/1‑150 (MQ=255)
                                                                                                                                       acacGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGtt             <  1:2905871/150‑1 (MQ=255)
                                                                                                                                         acGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGTTcc           <  1:5344909/150‑1 (MQ=255)
                                                                                                                                           ggTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCtggtgg                                                 >  2:4230232/1‑110 (MQ=255)
                                                                                                                                           ggTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCtggtgg                                                 <  1:4230232/110‑1 (MQ=255)
                                                                                                                                              ggAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGTTCCGAgcg      >  2:2172998/1‑150 (MQ=255)
                                                                                                                                                aGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGTTCCGAGCGCt    <  1:5014984/150‑1 (MQ=255)
                                                                                                                                                  cGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGTTCCGAGCGCTcc  <  2:2053346/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTCCGAGATCCGCAAGCGTGTCGCGGCAGGCGAGCGAGTGCTGGCGACCACGTTGACCAAACGCATGGCCGAGGACCTCAGCGATTACCTGGCCGACCACGACGTGCGGGTACGCTACCTGCACTCGGACATCGACACGGTGGAGCGGGTGGAGATTATCCGCGACCTGCGCCTGGGCACCTTCGACGTGCTGGTGGGCATCAACCTGCTGCGCGAGGGCCTGGACATGCCCGAGGTGTCGCTGGTGGCCATCCTCGATGCGGACAAGGAAGGTTTTCTGCGTTCCGAGCGCTCC  >  NC_002947/2238155‑2238450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: