Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,243,269 G→T 10.6% intergenic (+51/‑230) gltX → / → PP_t25 glutamate‑‑tRNA ligase/tRNA‑Ala

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,243,2690GT10.6% 184.7 / 5.6 66intergenic (+51/‑230)gltX/PP_t25glutamate‑‑tRNA ligase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base G (27/30);  new base T (4/4);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTT  >  NC_002947/2243122‑2243418
                                                                                                                                                   |                                                                                                                                                      
gACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTa                                                                                                                                                      <  1:4305072/150‑1 (MQ=255)
  cgcgCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTaaa                                                                                                                                                    <  2:749024/150‑1 (MQ=255)
   gcgcTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAAAGGTAAAt                                                                                                                                                   >  2:331275/1‑150 (MQ=255)
       tACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                               <  2:3299412/150‑1 (MQ=255)
       tACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                               <  1:3970138/150‑1 (MQ=255)
          cGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAatat                                                                                                                                            <  1:1864096/150‑1 (MQ=255)
           gCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                                           <  1:4972470/150‑1 (MQ=255)
                  ccAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATAGCAttag                                                                                                                                    >  2:4840815/1‑148 (MQ=255)
                    aGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGcc                                                                                                                                  <  1:5000915/150‑1 (MQ=255)
                      gCTCTGGACCTTCTGGGTGGTGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCgg                                                                                                                                <  1:1469085/150‑1 (MQ=255)
                      gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCgg                                                                                                                                <  1:1469020/150‑1 (MQ=255)
                      gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCgg                                                                                                                                <  1:1469175/150‑1 (MQ=255)
                               ctgctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGc                                                                                                                       <  1:2502560/150‑1 (MQ=255)
                                tgctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCGGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCt                                                                                                                      <  1:1037943/150‑1 (MQ=255)
                                       tggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGGGCCCGCGGATGCACTGGTCAATTAAAAATAGCATTAGCCCGCACCCCCCCGCCcaccg                                                                                                               >  2:4575142/1‑148 (MQ=255)
                                       gggggtgTCTAAGAAAGAAAACAAAGAGTGGTAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTACCCCGGGGGAGCGGGGGGGCCCGCGACTGAAAGGGTAAATTCACTAGCGATTTCGCCGGCACGCCCGCTCCCACAg                                                                                                               <  2:1083971/149‑1 (MQ=255)
                                         gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                               >  2:5432235/1‑116 (MQ=255)
                                         gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                               <  1:5432235/116‑1 (MQ=255)
                                                               agagTGGTAAATGCTGTTGGCTTCAATCGCGTTATTCTGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCTAATGCAATGGTAATTTCAATATCGCTTTCGGCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCgg                                                                                      <  2:923966/150‑1 (MQ=255)
                                                                gagTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCggg                                                                                     <  2:3955070/150‑1 (MQ=255)
                                                                   tGGGAATAGCTGTTGGATTCCATCGGCTGATTCAGCTTTGCACCTAACCATTGGGAGCGGGCGTGCACGCGAATGCTATTGTAAATTCACTATCGCTTTCGCCGGCAGGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAACAACCGGGGTa                                                                                  <  2:3120754/150‑1 (MQ=255)
                                                                       aaaaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGGGCCCCCCCAAGCCATGGTAAAAAAAATAAAACAATAGCCGGCCCCCCCCCGCCCACACGCCAAAAAAACCCCCAAAAAACCggggggggc                                                                               >  2:298818/1‑145 (MQ=255)
                                                                            cTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTaa                                                                         <  2:3526381/150‑1 (MQ=255)
                                                                               ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCg                                                                                                                                    >  2:2901984/1‑89 (MQ=255)
                                                                               ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCg                                                                                                                                    <  1:2901984/89‑1 (MQ=255)
                                                                                   cTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTg                                                                  <  2:1143981/150‑1 (MQ=255)
                                                                                     tCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGtt                                                                <  2:1884537/150‑1 (MQ=255)
                                                                                           gCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGGAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGGAGGGCGGTAAGTGGTTGTTATCtgt                                                          >  2:4046429/1‑150 (MQ=255)
                                                                                            ccTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCCAATGCCAAGGTAAAATCAATATAGCATTCGCCGGCACCCCCGCACCCACAGGCC‑AACAAAGCCCCAAAAAACCCGGGTAGGGCGGTAAGTGATTGTTATCtgtg                                                         >  2:3982134/1‑150 (MQ=255)
                                                                                                    aGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCCCGCATGCAAAGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCACCCCCACGCC‑AACAAAGCCCCAAAAAAACCGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAtt                                                 >  2:4021052/1‑150 (MQ=255)
                                                                                                          gCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGaa                                           <  1:3136915/150‑1 (MQ=255)
                                                                                                            aCCTAACCAGTGGGAGCGGGCGGGGCCCCGAAATCAATGGTAAATTAAAAATAGCATTCGCCCGGACCCCCGCACCCACAGGCC‑AACGAAGCCCCAAAAAAACGGGGTAGGGCGGTTAGTGTTTGTTATCTGTGTAAAAATTTTTGAata                                         >  2:3955024/1‑150 (MQ=255)
                                                                                                                         gtgggggCGTGCCCGCTATTTCAATTTTAAATTAATAATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTa                            <  1:2292854/146‑1 (MQ=255)
                                                                                                                         gcgggggCGTGCCCGCGAATGCAATGCAAAATTCAATCTCGCAGTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTa                            <  1:4168580/146‑1 (MQ=255)
                                                                                                                           gCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTCAGTGAGTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGt                          >  2:2872293/1‑150 (MQ=255)
                                                                                                                           gCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGt                          <  2:2308399/150‑1 (MQ=255)
                                                                                                                            ggggcgggcCCGACAATGAATTTTTAAATTCAATATCGCATGCGCCGCCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGtt                         <  1:5017953/149‑1 (MQ=255)
                                                                                                                             gggCGTGCCCGCGAATGCAATGTTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGttt                        <  2:355951/150‑1 (MQ=255)
                                                                                                                             gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGttt                        >  2:5141163/1‑150 (MQ=255)
                                                                                                                              ggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt                                                                   <  1:2339370/106‑1 (MQ=255)
                                                                                                                              ggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt                                                                   >  2:2339370/1‑106 (MQ=255)
                                                                                                                               gCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa                      >  1:3568427/1‑150 (MQ=255)
                                                                                                                                 gTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACa                    <  2:4276888/150‑1 (MQ=255)
                                                                                                                                  tGCCCGCGAATGCATGGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg                   <  1:3571011/150‑1 (MQ=255)
                                                                                                                                    ccggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACTGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                 >  2:3337017/4‑150 (MQ=25)
                                                                                                                                    ccggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTTAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                 >  1:1491/4‑150 (MQ=25)
                                                                                                                                    ccggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                 >  1:1564/4‑150 (MQ=25)
                                                                                                                                    cccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                 >  1:5366968/1‑150 (MQ=255)
                                                                                                                                    cccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                 >  1:3323567/1‑150 (MQ=255)
                                                                                                                                    cccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                 >  1:2522047/1‑150 (MQ=255)
                                                                                                                                     cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACTGGCC‑AACTAAGCCCCAAAAAACCGTGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                >  2:1803707/3‑150 (MQ=25)
                                                                                                                                     ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                >  1:1430931/1‑150 (MQ=255)
                                                                                                                                     ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                >  2:2704011/1‑150 (MQ=255)
                                                                                                                                     ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                >  2:2135900/1‑150 (MQ=255)
                                                                                                                                     ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTAGAATATTTTTGAAAATTAGTTTGACAGccc                >  1:1502032/1‑150 (MQ=255)
                                                                                                                                     ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTGATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                >  2:4915712/1‑150 (MQ=255)
                                                                                                                                       gcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTg              >  1:3391359/1‑150 (MQ=255)
                                                                                                                                          aaTGCATTGTTAAATTCAAGCTCGCCTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCaa           <  1:3322455/150‑1 (MQ=255)
                                                                                                                                          aaTGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCaa           <  2:809248/150‑1 (MQ=255)
                                                                                                                                             gCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc        >  2:1549105/1‑150 (MQ=255)
                                                                                                                                             gCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc        >  2:108725/1‑150 (MQ=255)
                                                                                                                                               attGGTAATTTCATTATCGCATTCGCGGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATTTTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGa      <  1:443707/148‑1 (MQ=255)
                                                                                                                                               aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGa      >  2:339460/1‑150 (MQ=255)
                                                                                                                                                aTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa     >  1:4286710/1‑150 (MQ=255)
                                                                                                                                                aTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa     <  1:2135900/150‑1 (MQ=255)
                                                                                                                                                 tGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAAc    <  2:3391359/150‑1 (MQ=255)
                                                                                                                                                   gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACtt  >  2:2353592/1‑150 (MQ=255)
                                                                                                                                                   gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACtt  >  2:2353503/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
GACGCGCTACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTT  >  NC_002947/2243122‑2243418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑