Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,243,278	A→C	8.5%	intergenic (+60/‑221)	gltX → / → PP_t25	glutamate‑‑tRNA ligase/tRNA‑Ala

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,243,278	0	A	C	8.5%	177.1 / 5.0	63	intergenic (+60/‑221)	gltX/PP_t25	glutamate‑‑tRNA ligase/tRNA‑Ala
Reads supporting (aligned to +/- strand):  ref base A (29/26);  new base C (4/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATAT  >  NC_002947/2243129‑2243423                                                                                                                                                      |                                                                                                                                                   tACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                                    <  2:3299412/150‑1 (MQ=255) tACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                                    <  1:3970138/150‑1 (MQ=255)    cGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAatat                                                                                                                                                 <  1:1864096/150‑1 (MQ=255)     gCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATc                                                                                                                                                <  1:4972470/150‑1 (MQ=255)            ccAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATAGCAttag                                                                                                                                         >  2:4840815/1‑148 (MQ=255)              aGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGcc                                                                                                                                       <  1:5000915/150‑1 (MQ=255)                gCTCTGGACCTTCTGGGTGGTGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCgg                                                                                                                                     <  1:1469085/150‑1 (MQ=255)                gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCgg                                                                                                                                     <  1:1469020/150‑1 (MQ=255)                gCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCgg                                                                                                                                     <  1:1469175/150‑1 (MQ=255)                         ctgctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGc                                                                                                                            <  1:2502560/150‑1 (MQ=255)                          tgctgGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCGGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCt                                                                                                                           <  1:1037943/150‑1 (MQ=255)                                 tggggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGGGCCCGCGGATGCACTGGTCAATTAAAAATAGCATTAGCCCGCACCCCCCCGCCcaccg                                                                                                                    >  2:4575142/1‑148 (MQ=255)                                 gggggtgTCTAAGAAAGAAAACAAAGAGTGGTAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTACCCCGGGGGAGCGGGGGGGCCCGCGACTGAAAGGGTAAATTCACTAGCGATTTCGCCGGCACGCCCGCTCCCACAg                                                                                                                    <  2:1083971/149‑1 (MQ=255)                                   gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                                    <  1:5432235/116‑1 (MQ=255)                                   gggtgTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCaa                                                                                                                                                    >  2:5432235/1‑116 (MQ=255)                                                         agagTGGTAAATGCTGTTGGCTTCAATCGCGTTATTCTGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCTAATGCAATGGTAATTTCAATATCGCTTTCGGCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCgg                                                                                           <  2:923966/150‑1 (MQ=255)                                                          gagTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCggg                                                                                          <  2:3955070/150‑1 (MQ=255)                                                             tGGGAATAGCTGTTGGATTCCATCGGCTGATTCAGCTTTGCACCTAACCATTGGGAGCGGGCGTGCACGCGAATGCTATTGTAAATTCACTATCGCTTTCGCCGGCAGGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAACAACCGGGGTa                                                                                       <  2:3120754/150‑1 (MQ=255)                                                                 aaaaGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGGGCCCCCCCAAGCCATGGTAAAAAAAATAAAACAATAGCCGGCCCCCCCCCGCCCACACGCCAAAAAAACCCCCAAAAAACCggggggggc                                                                                    >  2:298818/1‑145 (MQ=255)                                                                      cTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTaa                                                                              <  2:3526381/150‑1 (MQ=255)                                                                         ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCg                                                                                                                                         <  1:2901984/89‑1 (MQ=255)                                                                         ttGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCg                                                                                                                                         >  2:2901984/1‑89 (MQ=255)                                                                             cTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTg                                                                       <  2:1143981/150‑1 (MQ=255)                                                                               tCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGtt                                                                     <  2:1884537/150‑1 (MQ=255)                                                                                     gCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGGAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGGAGGGCGGTAAGTGGTTGTTATCtgt                                                               >  2:4046429/1‑150 (MQ=255)                                                                                      ccTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCCAATGCCAAGGTAAAATCAATATAGCATTCGCCGGCACCCCCGCACCCACAGGCC‑AACAAAGCCCCAAAAAACCCGGGTAGGGCGGTAAGTGATTGTTATCtgtg                                                              >  2:3982134/1‑150 (MQ=255)                                                                                              aGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCCCGCATGCAAAGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCACCCCCACGCC‑AACAAAGCCCCAAAAAAACCGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAAtt                                                      >  2:4021052/1‑150 (MQ=255)                                                                                                    gCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGaa                                                <  1:3136915/150‑1 (MQ=255)                                                                                                      aCCTAACCAGTGGGAGCGGGCGGGGCCCCGAAATCAATGGTAAATTAAAAATAGCATTCGCCCGGACCCCCGCACCCACAGGCC‑AACGAAGCCCCAAAAAAACGGGGTAGGGCGGTTAGTGTTTGTTATCTGTGTAAAAATTTTTGAata                                              >  2:3955024/1‑150 (MQ=255)                                                                                                                   gtgggggCGTGCCCGCTATTTCAATTTTAAATTAATAATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTa                                 <  1:2292854/146‑1 (MQ=255)                                                                                                                   gcgggggCGTGCCCGCGAATGCAATGCAAAATTCAATCTCGCAGTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTa                                 <  1:4168580/146‑1 (MQ=255)                                                                                                                     gCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTCAGTGAGTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGt                               >  2:2872293/1‑150 (MQ=255)                                                                                                                     gCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGt                               <  2:2308399/150‑1 (MQ=255)                                                                                                                      ggggcgggcCCGACAATGAATTTTTAAATTCAATATCGCATGCGCCGCCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGtt                              <  1:5017953/149‑1 (MQ=255)                                                                                                                       gggCGTGCCCGCGAATGCAATGTTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGttt                             <  2:355951/150‑1 (MQ=255)                                                                                                                       gggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGttt                             >  2:5141163/1‑150 (MQ=255)                                                                                                                        ggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt                                                                        <  1:2339370/106‑1 (MQ=255)                                                                                                                        ggCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGAtt                                                                        >  2:2339370/1‑106 (MQ=255)                                                                                                                         gCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGa                           >  1:3568427/1‑150 (MQ=255)                                                                                                                           gTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACa                         <  2:4276888/150‑1 (MQ=255)                                                                                                                            tGCCCGCGAATGCATGGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAg                        <  1:3571011/150‑1 (MQ=255)                                                                                                                              ccggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACTGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                      >  2:3337017/4‑150 (MQ=25)                                                                                                                              ccggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTTAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                      >  1:1491/4‑150 (MQ=25)                                                                                                                              ccggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                      >  1:1564/4‑150 (MQ=25)                                                                                                                              cccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                      >  1:5366968/1‑150 (MQ=255)                                                                                                                              cccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                      >  1:2522047/1‑150 (MQ=255)                                                                                                                              cccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGcc                      >  1:3323567/1‑150 (MQ=255)                                                                                                                               cggcgAATGCGATATTGAATTTACCATTGCATTCGCGGGCACGCCCGCTCCCACTGGCC‑AACTAAGCCCCAAAAAACCGTGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                     >  2:1803707/3‑150 (MQ=25)                                                                                                                               ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                     >  1:1430931/1‑150 (MQ=255)                                                                                                                               ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                     >  2:2135900/1‑150 (MQ=255)                                                                                                                               ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                     >  2:2704011/1‑150 (MQ=255)                                                                                                                               ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTAGAATATTTTTGAAAATTAGTTTGACAGccc                     >  1:1502032/1‑150 (MQ=255)                                                                                                                               ccGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTGATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGccc                     >  2:4915712/1‑150 (MQ=255)                                                                                                                                 gcgAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTg                   >  1:3391359/1‑150 (MQ=255)                                                                                                                                    aaTGCATTGTTAAATTCAAGCTCGCCTTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCaa                <  1:3322455/150‑1 (MQ=255)                                                                                                                                    aaTGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCaa                <  2:809248/150‑1 (MQ=255)                                                                                                                                       gCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc             >  2:1549105/1‑150 (MQ=255)                                                                                                                                       gCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATcc             >  2:108725/1‑150 (MQ=255)                                                                                                                                         attGGTAATTTCATTATCGCATTCGCGGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATTTTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGa           <  1:443707/148‑1 (MQ=255)                                                                                                                                         aaTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGa           >  2:339460/1‑150 (MQ=255)                                                                                                                                          aTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa          >  1:4286710/1‑150 (MQ=255)                                                                                                                                          aTGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGaa          <  1:2135900/150‑1 (MQ=255)                                                                                                                                           tGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAAc         <  2:3391359/150‑1 (MQ=255)                                                                                                                                             gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACtt       >  2:2353503/1‑150 (MQ=255)                                                                                                                                             gTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACtt       >  2:2353592/1‑150 (MQ=255)                                                                                                                                                aaTTCAATAGCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAat    <  1:331275/150‑1 (MQ=255)                                                                                                                                                  ttCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAatat  >  1:533056/1‑150 (MQ=255)                                                                                                                                                  ttCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAatat  >  1:3324850/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                   TACCGCCTGCGCCAGGCTCTGGACCTGCTGGGTGGGGTGTCGAAGAAAGAAAACAAAGAGTGGGAAAAGCTGTTGGCTTCCATCGCCTGATTCAGCTTTGCACCTAACCAGTGGGAGCGGGCGTGCCCGCGAATGCAATGGTAAATTCAATATCGCATTCGCCGGCACGCCCGCTCCCACAGGCC‑AACTAAGCCCCAAAAAACCGGGGTAGGGCGGTAAGTGATTGTTATCTGTGAAAAAATTTTTGAATATTTTTGAAAATTAGTTTGACAGCCCTGCAATCCGAACTTAATAT  >  NC_002947/2243129‑2243423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑