Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,253,217 A→G 6.6% K195E (AAG→GAG)  leuC → 3‑isopropylmalate dehydratase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,253,2170AG 6.6% 616.7 / 20.4 196K195E (AAG→GAG) leuC3‑isopropylmalate dehydratase large subunit
Reads supporting (aligned to +/- strand):  ref base A (103/80);  new base G (7/6);  total (110/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 201 total aligned reads displayed.

TGGCCCATGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACC  >  NC_002947/2253068‑2253366
                                                                                                                                                     |                                                                                                                                                     
 ggCCCATGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCaa                                                                                                                                                      <  1:2353675/150‑1 (MQ=255)
   cccATGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACCGGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGa                                                                                                                                                    <  2:1117929/150‑1 (MQ=255)
    ccATGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGag                                                                                                                                                   >  1:3563637/1‑149 (MQ=255)
    ccATGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGAt                                                                                                                                                   >  2:2790302/1‑150 (MQ=255)
      aTGGCATTGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCg                                                                                                                                                 >  1:2769555/1‑150 (MQ=255)
       tGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCgg                                                                                                                                                >  2:1397/1‑150 (MQ=255)
            tCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCAccg                                                                                                                                           >  1:183868/1‑150 (MQ=255)
                 aCTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGCTGCCGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGt                                                                                                                                      <  2:2342470/150‑1 (MQ=255)
                 aCTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGt                                                                                                                                      <  1:3255299/150‑1 (MQ=255)
                  cTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTg                                                                                                                                     <  1:4536552/150‑1 (MQ=255)
                  cTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTg                                                                                                                                     <  2:5398601/150‑1 (MQ=255)
                   ttCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTgg                                                                                                                                    >  2:1695871/1‑150 (MQ=255)
                   ttCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTgg                                                                                                                                    >  1:82142/1‑150 (MQ=255)
                   ttCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATACTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTg                                                                                                                                     <  2:1245000/149‑1 (MQ=255)
                   ttCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATACTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTg                                                                                                                                     >  1:1245000/1‑149 (MQ=255)
                      cGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCac                                                                                                                                 >  1:5051733/1‑149 (MQ=255)
                      cGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCaa                                                                                                                                 >  1:5164723/1‑150 (MQ=255)
                      cGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCaa                                                                                                                                 >  1:904150/1‑150 (MQ=255)
                      cGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCaa                                                                                                                                 <  1:1316264/150‑1 (MQ=255)
                       gAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAAc                                                                                                                                >  2:4518048/1‑150 (MQ=255)
                       gAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAAc                                                                                                                                <  2:4454197/150‑1 (MQ=255)
                         ggTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACgg                                                                                                                              <  2:669494/150‑1 (MQ=255)
                           tCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTc                                                                                                                            >  2:2850897/1‑150 (MQ=255)
                             gAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCAc                                                                                                                          >  2:3325037/1‑150 (MQ=255)
                                  cGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCAt                                                                                                                     <  2:2936032/150‑1 (MQ=255)
                                   gTGCTCGCCACCTAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATg                                                                                                                    >  2:255864/1‑150 (MQ=255)
                                   gTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATg                                                                                                                    <  1:3806149/150‑1 (MQ=255)
                                     gCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGACGAACATGCTGGTGCGCGTGGAAGGCCAAGTGCCTGCCGGCGTCACCGCCAAGGATGTCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGa                                                                                                                  <  2:3142196/150‑1 (MQ=255)
                                     gCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGa                                                                                                                  >  2:1608651/1‑150 (MQ=255)
                                        cGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAAtt                                                                                                               >  1:2924613/1‑150 (MQ=255)
                                         gCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTc                                                                                                              >  2:810104/1‑150 (MQ=255)
                                         gCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTc                                                                                                              >  1:1020721/1‑150 (MQ=255)
                                         gCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTc                                                                                                              >  2:102707/1‑150 (MQ=255)
                                         gCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTc                                                                                                              >  2:4915589/1‑150 (MQ=255)
                                            aCCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgca                                                                                                           >  2:3458641/1‑150 (MQ=255)
                                             cccAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcag                                                                                                          <  2:2417318/150‑1 (MQ=255)
                                             cccAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcag                                                                                                          <  2:4976091/150‑1 (MQ=255)
                                               cAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcaggc                                                                                                        >  1:212127/1‑150 (MQ=255)
                                                cgTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGTTATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgcaggca                                                                                                       <  1:1660646/149‑1 (MQ=255)
                                                      tGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCa                                                                                                 <  2:183868/150‑1 (MQ=255)
                                                         tCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATAGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTc                                                                                              >  2:194256/1‑150 (MQ=255)
                                                           gCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTcgc                                                                                            >  2:4670855/1‑150 (MQ=255)
                                                           gCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTcgc                                                                                            <  1:2791138/150‑1 (MQ=255)
                                                             cAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGa                                                                                          >  2:2676577/1‑150 (MQ=255)
                                                                gaagaTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAAtt                                                                                       >  1:1772052/1‑150 (MQ=255)
                                                                   gaTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTc                                                                                    >  2:715292/1‑150 (MQ=255)
                                                                   gaTGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTc                                                                                    >  1:2239236/1‑150 (MQ=255)
                                                                      gaagaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGAt                                                                                 >  1:5060792/1‑150 (MQ=255)
                                                                      gaagaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCAGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGAt                                                                                 >  2:3712306/1‑150 (MQ=255)
                                                                       aagaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATg                                                                                <  2:2704779/150‑1 (MQ=255)
                                                                       aagaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATg                                                                                <  1:1937616/150‑1 (MQ=255)
                                                                       aagaaCATGCTGGTGCGCGTGGAAGGCCAAGTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATg                                                                                <  2:2577752/150‑1 (MQ=255)
                                                                         gaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGa                                                                              <  1:3209109/150‑1 (MQ=255)
                                                                         gaaCATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGa                                                                              >  2:3690964/1‑150 (MQ=255)
                                                                            cATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAgg                                                                           <  1:4176084/150‑1 (MQ=255)
                                                                                 tGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCa                                                                      >  1:4794700/1‑150 (MQ=255)
                                                                                 tGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCa                                                                      <  1:4153205/150‑1 (MQ=255)
                                                                                  ggTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCAt                                                                     <  2:4065790/150‑1 (MQ=255)
                                                                                  ggTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCAt                                                                     <  2:5451051/150‑1 (MQ=255)
                                                                                   gTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgca                                                                                                           >  1:2500752/1‑111 (MQ=255)
                                                                                   gTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCgca                                                                                                           <  2:2500752/111‑1 (MQ=255)
                                                                                       gcgTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGTACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCa                                                                <  1:2398399/150‑1 (MQ=255)
                                                                                        cgTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCAt                                                               <  2:5164723/150‑1 (MQ=255)
                                                                                         gTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATc                                                              >  2:4375262/1‑150 (MQ=255)
                                                                                          tGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCt                                                             <  2:521733/150‑1 (MQ=255)
                                                                                                   aaTTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGt                                                    <  1:4586151/150‑1 (MQ=255)
                                                                                                    aTTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTc                                                   <  1:3690964/150‑1 (MQ=255)
                                                                                                     ttGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTcc                                                  <  1:2847055/150‑1 (MQ=255)
                                                                                                       gcctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCAt                                                <  1:4859381/150‑1 (MQ=255)
                                                                                                       gcctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCAt                                                >  1:215963/1‑150 (MQ=255)
                                                                                                        gctccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGAtt                                               >  1:2424002/7‑149 (MQ=255)
                                                                                                        cctgccGGCGTCACCGCCAAGGATATCGTGCTGACCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATc                                               <  2:4890160/150‑1 (MQ=255)
                                                                                                         ctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAAtt                                                                                       >  1:151245/1‑109 (MQ=255)
                                                                                                         ctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCg                                              >  1:5426902/1‑150 (MQ=255)
                                                                                                           gccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGaa                                            <  1:3936603/150‑1 (MQ=255)
                                                                                                           ccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGaa                                            <  1:3107287/147‑1 (MQ=255)
                                                                                                            caggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAg                                           >  1:2713888/3‑150 (MQ=255)
                                                                                                             cGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGc                                          >  2:2720067/1‑150 (MQ=255)
                                                                                                             cGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGc                                          >  1:4816019/1‑150 (MQ=255)
                                                                                                              ggCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGcc                                         >  2:3909052/1‑150 (MQ=255)
                                                                                                                cGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAGGCgg                                       <  2:4260560/150‑1 (MQ=255)
                                                                                                                  tCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAGgcggcg                                     >  1:2670278/1‑150 (MQ=255)
                                                                                                                    aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                   >  2:775167/1‑150 (MQ=255)
                                                                                                                         cAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAGGCGGCGCAcgcgc                              >  2:5039710/1‑149 (MQ=255)
                                                                                                                             gATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGc                          >  1:3151939/1‑150 (MQ=255)
                                                                                                                             gATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGc                          <  1:1978670/150‑1 (MQ=255)
                                                                                                                              atatCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGcc                         >  1:1396095/1‑150 (MQ=255)
                                                                                                                              atatCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGcc                         <  2:5006160/150‑1 (MQ=255)
                                                                                                                               tatCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCt                        >  1:2217309/1‑150 (MQ=255)
                                                                                                                                   gTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggtg                    <  2:3580266/150‑1 (MQ=255)
                                                                                                                                    tGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggtgg                   <  1:3413422/150‑1 (MQ=255)
                                                                                                                                       tGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCac                >  1:4310338/1‑149 (MQ=255)
                                                                                                                                       tGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCaa                <  2:1492310/150‑1 (MQ=255)
                                                                                                                                       tGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCaa                >  2:2386162/1‑150 (MQ=255)
                                                                                                                                         gCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAAcc              >  1:1598716/1‑150 (MQ=255)
                                                                                                                                           cGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGa            <  1:4893069/150‑1 (MQ=255)
                                                                                                                                           cGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGa            >  2:580451/1‑150 (MQ=255)
                                                                                                                                             tGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATg          <  1:2600310/150‑1 (MQ=255)
                                                                                                                                                   gCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTa    >  1:622027/1‑150 (MQ=255)
                                                                                                                                                    cAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTAc   >  2:3175045/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGGCCCATGGCATCGGCACTTCCGAGGTCGAGCACGTGCTCGCCACCCAGTGCCTGGTCGCCAAGAAGATGAAGAACATGCTGGTGCGCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACC  >  NC_002947/2253068‑2253366

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑