Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,253,304 A→G 6.0% I224V (ATC→GTC)  leuC → 3‑isopropylmalate dehydratase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,253,3040AG 6.0% 635.0 / 17.9 200I224V (ATC→GTC) leuC3‑isopropylmalate dehydratase large subunit
Reads supporting (aligned to +/- strand):  ref base A (86/102);  new base G (7/5);  total (93/107)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

Only 100 of 203 total aligned reads displayed.

GCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTGGTGTCGGAT  >  NC_002947/2253155‑2253453
                                                                                                                                                     |                                                                                                                                                     
 cgTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCAt                                                                                                                                                      <  2:5164723/150‑1 (MQ=255)
  gTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATc                                                                                                                                                     >  2:4375262/1‑150 (MQ=255)
  gTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATc                                                                                                                                                     >  1:1654717/1‑150 (MQ=255)
  gTGGAAGGCCAAGTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATc                                                                                                                                                     <  1:3410174/150‑1 (MQ=255)
   tGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCt                                                                                                                                                    <  2:521733/150‑1 (MQ=255)
        ggCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAAc                                                                                                                                               <  1:666870/150‑1 (MQ=255)
          ccAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACAt                                                                                                                                             <  2:2606042/150‑1 (MQ=255)
                gcctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCAt                                                                                                                                       <  1:4859381/150‑1 (MQ=255)
                gcctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCAt                                                                                                                                       >  1:215963/1‑150 (MQ=255)
                 gctccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGAtt                                                                                                                                      <  2:2693456/144‑2 (MQ=255)
                 gctccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGAtt                                                                                                                                      >  1:2424002/7‑149 (MQ=255)
                 cctgccGGCGTCACCGCCAAGGATATCGTGCTGACCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATc                                                                                                                                      <  2:4890160/150‑1 (MQ=255)
                  ctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCg                                                                                                                                     >  1:4178621/1‑150 (MQ=255)
                  ctgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCg                                                                                                                                     >  1:5426902/1‑150 (MQ=255)
                   tgccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGa                                                                                                                                    >  1:1091266/1‑150 (MQ=255)
                    gccGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGaa                                                                                                                                   <  1:3936603/150‑1 (MQ=255)
                    ccaggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGaa                                                                                                                                   <  1:3107287/147‑1 (MQ=255)
                     caggCGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAg                                                                                                                                  >  1:2713888/3‑150 (MQ=255)
                      cGGCGTCACCGCCAAGGGTATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGc                                                                                                                                 >  1:4815848/1‑150 (MQ=255)
                      cGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGc                                                                                                                                 >  2:2720067/1‑150 (MQ=255)
                       ggCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGcc                                                                                                                                >  2:3909052/1‑150 (MQ=255)
                         cGTCACCGCCAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAGGCgg                                                                                                                              <  2:4260560/150‑1 (MQ=255)
                          gTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGgc                                                                                                                             >  2:4411669/1‑150 (MQ=255)
                             aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                                                                                                          >  2:4941209/1‑150 (MQ=255)
                             aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                                                                                                          >  2:3510252/1‑150 (MQ=255)
                             aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                                                                                                          >  2:775167/1‑150 (MQ=255)
                             aCCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGcc                                                                                                                          >  2:2532170/1‑150 (MQ=255)
                                  cAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGt                                                                                                                     <  1:1583570/150‑1 (MQ=255)
                                  cAAGGACATCACGCTTGCAATCATTGGCGAGATTGGCACGGCTGGCGGCACAGGCTATGTGATCGAATATGCTGGCAGCGCCATTCGCTCGCTGTCGATGGAAGGCCGCATGACGGTCTGCAACATGTCGATTGAAGGCGGCGCAcgcgc                                                                                                                     >  2:5039710/1‑149 (MQ=255)
                                      gATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGc                                                                                                                 >  1:3151939/1‑150 (MQ=255)
                                      gATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGc                                                                                                                 <  1:1978670/150‑1 (MQ=255)
                                       atatCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGcc                                                                                                                >  1:1396095/1‑150 (MQ=255)
                                        tatCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCt                                                                                                               >  1:2217676/1‑150 (MQ=255)
                                          tCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtgg                                                                                                             >  2:5438251/1‑150 (MQ=255)
                                          tCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtgg                                                                                                             <  1:2506448/150‑1 (MQ=255)
                                            gTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggtg                                                                                                           <  2:3580266/150‑1 (MQ=255)
                                            gTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCtggtg                                                                                                           <  1:4652989/150‑1 (MQ=255)
                                                tGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCCTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCac                                                                                                       >  1:4310338/1‑149 (MQ=255)
                                                tGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCaa                                                                                                       <  2:1492310/150‑1 (MQ=255)
                                                tGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCaa                                                                                                       >  2:2386162/1‑150 (MQ=255)
                                                    cGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGa                                                                                                   <  2:3692108/150‑1 (MQ=255)
                                                    cGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGa                                                                                                   <  1:4893069/150‑1 (MQ=255)
                                                            gCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTa                                                                                           >  1:4574342/1‑150 (MQ=255)
                                                            gCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTa                                                                                           >  1:622008/1‑150 (MQ=255)
                                                             cAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTAc                                                                                          >  2:3175045/1‑150 (MQ=255)
                                                              aaGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTAcc                                                                                         >  2:689031/1‑150 (MQ=255)
                                                                 aTCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGtt                                                                                      <  2:2003621/150‑1 (MQ=255)
                                                                 aTCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGtt                                                                                      >  1:1841286/1‑150 (MQ=255)
                                                                  tCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTg                                                                                     <  2:296449/150‑1 (MQ=255)
                                                                   cGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGc                                                                                    <  2:2239236/150‑1 (MQ=255)
                                                                   cGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGc                                                                                    <  1:5381208/150‑1 (MQ=255)
                                                                   cGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGc                                                                                    <  1:4010527/150‑1 (MQ=255)
                                                                       aCCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTAc                                                                                >  1:68924/1‑150 (MQ=255)
                                                                             ggTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGaa                                                                          <  1:2850897/150‑1 (MQ=255)
                                                                               tGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAgg                                                                        >  1:2662832/1‑150 (MQ=255)
                                                                               tGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAgg                                                                        >  1:4572114/1‑150 (MQ=255)
                                                                                 gCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGgc                                                                      <  1:45354/150‑1 (MQ=255)
                                                                                      ggTCACCCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCg                                                                 >  2:3284126/1‑150 (MQ=255)
                                                                                        tCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTa                                                               >  2:4696836/1‑150 (MQ=255)
                                                                                           cGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTAcgc                                                            <  2:1020721/150‑1 (MQ=255)
                                                                                           cGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTAcgc                                                            <  1:4518048/150‑1 (MQ=255)
                                                                                             ccATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACgcgc                                                          <  1:1695871/150‑1 (MQ=255)
                                                                                              cATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGcc                                                         >  2:1050475/1‑150 (MQ=255)
                                                                                                tGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTATGCGCCGa                                                       >  2:2753210/1‑150 (MQ=255)
                                                                                                tGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGa                                                       >  1:4763392/1‑150 (MQ=255)
                                                                                                  gAATTCGCAGGCAGTGCCATTCGCGACTTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAg                                                     <  2:1772052/150‑1 (MQ=255)
                                                                                                    aTTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAggg                                                   >  2:3533745/1‑150 (MQ=255)
                                                                                                     ttCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGc                                                  >  2:143958/1‑150 (MQ=255)
                                                                                                     ttCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGc                                                  <  1:3458641/150‑1 (MQ=255)
                                                                                                       cGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGa                                                <  1:1502645/150‑1 (MQ=255)
                                                                                                        gcaggcagTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAg                                               <  2:2924538/150‑1 (MQ=255)
                                                                                                            gcagTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGt                                           <  1:3717107/150‑1 (MQ=255)
                                                                                                            gcagTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGt                                           <  1:2149858/150‑1 (MQ=255)
                                                                                                            gcagTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGt                                           >  1:3776445/1‑150 (MQ=255)
                                                                                                                tGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaa                                       <  2:2456377/150‑1 (MQ=255)
                                                                                                                tGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaa                                       <  1:102707/150‑1 (MQ=255)
                                                                                                                tGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaa                                       <  1:89457/150‑1 (MQ=255)
                                                                                                                tGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaa                                       <  1:1863059/150‑1 (MQ=255)
                                                                                                                    aTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTAc                                                                                          <  2:808614/95‑1 (MQ=255)
                                                                                                                    aTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaagcaa                                   >  1:3188591/1‑150 (MQ=255)
                                                                                                                    aTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaagcaa                                   <  2:3992420/150‑1 (MQ=255)
                                                                                                                    aTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaagcaa                                   >  1:1931125/1‑150 (MQ=255)
                                                                                                                    aTTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGaagcaa                                   <  1:4915589/150‑1 (MQ=255)
                                                                                                                      tCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTGGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGTAGTGGaagcaagc                                 <  2:2468756/150‑1 (MQ=255)
                                                                                                                        gcgAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCgg                               >  1:3052327/1‑150 (MQ=255)
                                                                                                                        gcgAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCgg                               >  2:1156830/1‑150 (MQ=255)
                                                                                                                          gAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTc                             <  2:212288/150‑1 (MQ=255)
                                                                                                                            aTTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGa                           <  2:5060792/150‑1 (MQ=255)
                                                                                                                             ttGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAg                          <  1:2676577/150‑1 (MQ=255)
                                                                                                                                      gAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGaaa                 <  1:1608651/150‑1 (MQ=255)
                                                                                                                                      gAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGaaa                 <  2:1307697/150‑1 (MQ=255)
                                                                                                                                         ggCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGAc              >  2:314085/1‑150 (MQ=255)
                                                                                                                                           ccGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCt            <  1:741307/150‑1 (MQ=255)
                                                                                                                                            cGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTg           <  1:810104/150‑1 (MQ=255)
                                                                                                                                            cGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTg           <  2:163872/150‑1 (MQ=255)
                                                                                                                                             gCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTgg          >  1:5114224/1‑150 (MQ=255)
                                                                                                                                                tGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTGgtgt       <  1:255864/150‑1 (MQ=255)
                                                                                                                                                tGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTGgtgt       <  2:533071/150‑1 (MQ=255)
                                                                                                                                                 gACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTGGTGTc      >  1:524778/1‑150 (MQ=255)
                                                                                                                                                     aTCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTGGTGTCGGAt  <  1:4375262/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGTGGAAGGCCAATTGCCTGCCGGCGTCACCGCCAAGGATATCGTGCTGGCCGTGATCGGCAAGATCGGCACCGCCGGTGGCAACGGTCACGCCATGGAATTCGCAGGCAGTGCCATTCGCGAATTGTCGATGGAAGGCCGCATGACCATCTGCAACATGTCCATCGAAGCCGGCGCCCGCGTGGGCCTGGTGGCAACCGATGCCACTACCGTTGCCTACGTCGAAGGGCGCCCGTACGCGCCGAAGGGCGAGCAGTGGAAGCAAGCGGTCGAGTCGTGGAAAGACCTGGTGTCGGAT  >  NC_002947/2253155‑2253453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑