BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,273,631	C→T	9.7%	intergenic (+50/‑50)	PP_t32 → / → PP_t33	tRNA‑Asp/tRNA‑Asp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,273,631	0	C	T	9.7%	404.0 / 24.8	146	intergenic (+50/‑50)	PP_t32/PP_t33	tRNA‑Asp/tRNA‑Asp
Reads supporting (aligned to +/- strand): ref base C (71/60); new base T (7/7); total (79/67)
Fisher's exact test for biased strand distribution p-value = 7.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 153 total aligned reads displayed.

CCAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGA  >  NC_002947/2273486‑2273779

ccAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATATTACTGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACACTGCACAGGc                                                                                                                                                  >  2:4761942/1‑150 (MQ=21)

  agagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCt                                                                                                                                                <  1:4799679/150‑1 (MQ=255)

  agagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTACAGTAAAAGATCCTAGAAAGGCACCGGTCAGAAGCAACAAGGCACAGGCCt                                                                                                                                                >  2:5264023/1‑150 (MQ=17)

   gagagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCtt                                                                                                                                               <  1:3637142/150‑1 (MQ=255)

    agagGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTg                                                                                                                                              <  2:4796804/150‑1 (MQ=255)

       ggAACGCACTGCGCAGCGGTAGTTCAGTCGGTTTGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCATGTGCCTTgtgg                                                                                                                                           <  2:2745856/150‑4 (MQ=255)

        gAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCt                                                                                                                                          >  1:3146864/1‑150 (MQ=255)

         aaCGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTGATTAACTGTTGGTTGACTTGCACCGGTGTTAATCCAGAAGGGAGATGCATTGGGGTg                                                                                                                                         >  2:2078134/1‑150 (MQ=17)

              aCTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTCACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATccc                                                                                                                                    >  1:3365009/1‑150 (MQ=255)

              aCTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGAAGGGTGACACGCAACGGGATCAAGCCACAAGCCACATGCCTTGGGCTGATccc                                                                                                                                    >  1:1994659/1‑150 (MQ=18)

               cTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCa                                                                                                                                   <  2:719925/150‑1 (MQ=255)

                  cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCATGTGCCTTgtggcttc                                                                                                                                       >  2:2355170/1‑136 (MQ=255)

                  cgcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCATGTGCCTTgtggcttc                                                                                                                                       <  1:2355170/143‑8 (MQ=255)

                   gcAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGttt                                                                                                                               <  2:854491/150‑1 (MQ=255)

                           aGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCggg                                                                                                                       <  1:1901484/150‑1 (MQ=255)

                             ttCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGgcg                                                                                                                     <  2:1228675/150‑1 (MQ=255)

                                     gTTAGAATACCGGCCTATCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTg                                                                                                             >  2:2926590/1‑150 (MQ=255)

                                       tAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGaa                                                                                                           <  1:1521169/150‑1 (MQ=255)

                                        aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACCGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAAc                                                                                                          <  2:3705114/150‑1 (MQ=255)

                                        aGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAAc                                                                                                          <  2:3705110/150‑1 (MQ=255)

                                           cccccGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTACAGTCACAGAAGGTAGAAAGCCACCGGTCTGAAGCAACAAGGCACATGCCTTGGGCTGAACCAAGTTTCTATCGGGCGCAAGCATGTACGag                                                                                                       >  2:447584/4‑149 (MQ=21)

                                           aTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGAt                                                                                                       <  2:812663/150‑1 (MQ=255)

                                           aTACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGAt                                                                                                       <  2:3286203/150‑1 (MQ=255)

                                            tACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATa                                                                                                      >  2:1368450/1‑150 (MQ=255)

                                            ccccGGCGTGACAGGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATa                                                                                                      >  2:3507796/3‑150 (MQ=255)

                                               cGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATGTTGCAGTAAAGGAGGGGTGCCTGGCACGGGTGTGAAGCCACGAGGCACTGGCCTTGGGCTGTTCCCAGTTGCAGGGGGGCGCAGGCCTGAACGATACGc                                                                                                   >  2:4875338/1‑150 (MQ=21)

                                                 gCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAg                                                                                                 >  2:3123652/1‑150 (MQ=255)

                                                   cTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCg                                                                                               <  1:1420091/150‑1 (MQ=255)

                                                   cTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCg                                                                                               <  1:2168153/150‑1 (MQ=255)

                                                     gTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGt                                                                                             <  2:2997783/150‑1 (MQ=255)

                                                     gTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGt                                                                                             >  2:3828096/1‑150 (MQ=255)

                                                      tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTa                                                                                            <  1:2275361/150‑1 (MQ=255)

                                                      tCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTa                                                                                            <  2:2470365/150‑1 (MQ=255)

                                                        aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGcaca                                                                                                                                                     >  1:5318612/1‑91 (MQ=255)

                                                        aCGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGcaca                                                                                                                                                     <  2:5318612/91‑1 (MQ=255)

                                                           ccGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCa                                                                                       <  2:2865407/150‑1 (MQ=255)

                                                              ggggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCC‑CAAGGCATGTGCCTTgtggc                                                                                                                                          >  1:2106136/1‑91 (MQ=37)

                                                              ggggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCC‑CAAGGCATGTGCCTTgtggc                                                                                                                                          <  2:2106151/95‑5 (MQ=37)

                                                              ggggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCC‑CAAGGCATGTGCCTTgtggc                                                                                                                                          >  1:2106151/1‑91 (MQ=37)

                                                                ggTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCgg                                                                                  <  1:2268022/150‑1 (MQ=255)

                                                                 gTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGt                                                                                 <  2:1626440/150‑1 (MQ=255)

                                                                  tCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGtt                                                                                >  1:4342809/1‑150 (MQ=255)

                                                                  tCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGtt                                                                                >  1:4342761/1‑150 (MQ=255)

                                                                  tCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGtt                                                                                >  1:270434/1‑150 (MQ=255)

                                                                   cgcgGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                               <  1:684857/150‑1 (MQ=255)

                                                                        gTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATa                                                                          >  2:1621853/1‑150 (MQ=255)

                                                                           cGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCg                                                                       <  1:4575234/150‑1 (MQ=255)

                                                                            gAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCgg                                                                      >  1:2874748/1‑150 (MQ=255)

                                                                             aGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGc                                                                     <  2:3056754/150‑1 (MQ=255)

                                                                             aGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGc                                                                     <  2:3346880/150‑1 (MQ=255)

                                                                             aGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGAGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGc                                                                     >  2:1134595/1‑150 (MQ=255)

                                                                               tCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                               <  2:3423055/138‑1 (MQ=255)

                                                                               tCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                               >  1:3423055/1‑138 (MQ=255)

                                                                               tCCCGTCCGCTGCGCCATTTTTCAGTAACAGATCGGTGCCTGCCACCGGTCTGAAGCCACAAGGCACATGCCTTGGTCTGATCCCATTTTCAATCGCGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTAAGAATaaaggcat                                                                   >  1:2290709/1‑143 (MQ=37)

                                                                                 ccGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGGACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGt                                                                 >  2:5429628/1‑150 (MQ=255)

                                                                                  cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                               >  1:184467/1‑135 (MQ=255)

                                                                                  cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTa                                                                               <  2:184467/135‑1 (MQ=255)

                                                                                  cGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTc                                                                >  1:4519820/1‑150 (MQ=255)

                                                                                        cTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATAc                                                                         <  2:4796984/135‑1 (MQ=255)

                                                                                         tGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCACTCGGGCGCAAGCCTGAACGATGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                         <  2:847905/150‑1 (MQ=255)

                                                                                             ccATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGtt                                                                                         >  1:1664161/1‑114 (MQ=255)

                                                                                              cATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTc                                                    >  2:1280348/1‑150 (MQ=255)

                                                                                                  tttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCgg                                                <  1:1117425/150‑1 (MQ=255)

                                                                                                  tttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCgg                                                <  1:1117337/150‑1 (MQ=255)

                                                                                                  tttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCgg                                                <  1:2201670/150‑1 (MQ=255)

                                                                                                   ttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATcc                                                                                                                                     <  2:476299/64‑1 (MQ=255)

                                                                                                   ttCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATcc                                                                                                                                     >  1:476299/1‑64 (MQ=255)

                                                                                                      aGTAACGGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTc                                            >  2:3658666/1‑150 (MQ=255)

                                                                                                      aGTAACGGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTc                                            >  2:3658663/1‑150 (MQ=255)

                                                                                                      aGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTc                                            <  1:3123652/150‑1 (MQ=255)

                                                                                                       gTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCg                                           >  1:1523226/1‑150 (MQ=255)

                                                                                                        tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCACTCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                          <  1:3828096/150‑1 (MQ=255)

                                                                                                        tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                          >  1:279241/1‑150 (MQ=255)

                                                                                                        tAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                          <  1:2633829/150‑1 (MQ=255)

                                                                                                         aaCAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAg                                         >  1:5081940/1‑150 (MQ=255)

                                                                                                          aCAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGt                                        >  1:5449863/1‑150 (MQ=255)

                                                                                                             gATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTccc                                     >  1:2570990/1‑150 (MQ=255)

                                                                                                                  gTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                         >  1:4968655/1‑125 (MQ=255)

                                                                                                                  gTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCgg                                                         <  2:4968655/125‑1 (MQ=255)

                                                                                                                  gTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGTTCGCGGGTTCGAGTCCCGTCCg                                >  2:260731/1‑150 (MQ=255)

                                                                                                                       tGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTCGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgcg                           >  1:4781510/1‑150 (MQ=255)

                                                                                                                       tGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgcg                           >  2:1015217/1‑150 (MQ=255)

                                                                                                                        ggCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTgcgc                          >  1:5158139/1‑150 (MQ=255)

                                                                                                                             cGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCt                     >  1:2049463/1‑150 (MQ=255)

                                                                                                                               gTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGa                                          <  1:273878/127‑1 (MQ=255)

                                                                                                                                   gAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGttt               >  2:1893111/1‑150 (MQ=255)

                                                                                                                                      gCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCaa            <  1:3658663/150‑1 (MQ=255)

                                                                                                                                        cccaAGGCATGTGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGaa                                                                                                           <  2:2704549/51‑1 (MQ=25)

                                                                                                                                        cccaAGGCATGTGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCTGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAgg          >  1:2706534/3‑150 (MQ=255)

                                                                                                                                        cccaAGGCATGTGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACAATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCgg                                                >  2:1213557/3‑112 (MQ=255)

                                                                                                                                        cccaAGGCATGTGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACAATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCgg                                                <  1:1213557/110‑1 (MQ=255)

                                                                                                                                         acaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCa                                                                                       <  1:3862655/72‑1 (MQ=255)

                                                                                                                                         acaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGc                                                            <  2:2062896/99‑1 (MQ=255)

                                                                                                                                         acaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGc                                                            >  1:2062896/1‑99 (MQ=255)

                                                                                                                                         acaAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGt         >  1:722118/1‑150 (MQ=255)

                                                                                                                                           aaGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTcc       <  1:3663604/150‑1 (MQ=255)

                                                                                                                                            aGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTccc      >  2:4282788/1‑150 (MQ=255)

                                                                                                                                              gctcaTGCCTTGGGCTGATCGAAGTTTCATTCTGGCTCAATCGTTATCTATTCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTa    <  1:928266/147‑1 (MQ=17)

                                                                                                                                              gCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTa    <  1:928307/150‑1 (MQ=255)

                                                                                                                                                acaTGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCCGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGa  >  1:4809951/1‑150 (MQ=255)

CCAGAGAGGAACGCACTGCGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATTTTTCAGTAACAGATGGGTGCCTGGCACCGGTCTGAAGCCACAAGGCACATGCCTTGGGCTGATCCCAGTTTCAATCGGGCGCAAGCCTGAACGATACGCAGCGGTAGTTCAGTCGGTTAGAATACCGGCCTGTCACGCCGGGGGTCGCGGGTTCGAGTCCCGTCCGCTGCGCCATCTTCGTTTCAAGGTCCCTAGA  >  NC_002947/2273486‑2273779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`