BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,347,291	C→A	5.8%	G8* (GGA→TGA) ‡	PP_2062 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,347,291	0	C	A	5.8%	608.4 / 17.9	183	G8* (GGA→TGA) ‡	PP_2062	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (99/73); new base A (8/3); total (107/76)
Fisher's exact test for biased strand distribution p-value = 3.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 183 total aligned reads displayed.

AGTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGACA  >  NC_002947/2347144‑2347438

 gTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAGCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTc                                                                                                                                                  >  2:1646075/1‑150 (MQ=255)

 gTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTc                                                                                                                                                  >  1:3298124/1‑150 (MQ=255)

      ctggctggCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATc                                                                                                                                             >  2:2253958/1‑150 (MQ=255)

       tggctggcCTGTTGTTGGGTCCGTGGATGTTAGTGCGGCGCCATGTTGGTCAAGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCTTG‑TTTTTAAAGGTGAGTCGAATTTTTATGCGTTGACATATAACTGACTCAGTCAGATCt                                                                                                                                            >  2:2296724/1‑150 (MQ=255)

       tggctggcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCt                                                                                                                                            >  2:1338100/1‑150 (MQ=255)

       tggctggcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCt                                                                                                                                            >  1:2773591/1‑150 (MQ=255)

         gctggcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTgg                                                                                                                                          >  2:4135506/1‑150 (MQ=255)

             gcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACt                                                                                                                                      >  2:5042912/1‑150 (MQ=255)

             gcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACt                                                                                                                                      >  2:3727811/1‑150 (MQ=255)

             gcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACt                                                                                                                                      >  1:3815139/1‑150 (MQ=255)

             gcCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACt                                                                                                                                      <  1:2979194/150‑1 (MQ=255)

                   tgttgGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCAt                                                                                                                                <  1:3418788/150‑1 (MQ=255)

                     ttgGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCa                                                                                                                              <  2:4568788/150‑1 (MQ=255)

                     ttgGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCa                                                                                                                              >  1:2575752/1‑150 (MQ=255)

                        gggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTg                                                                                                                           <  1:2710894/150‑1 (MQ=255)

                         ggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGa                                                                                                                          <  1:5162635/150‑1 (MQ=255)

                         ggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGa                                                                                                                          <  2:1101564/150‑1 (MQ=255)

                         ggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGa                                                                                                                          <  1:2434896/150‑1 (MQ=255)

                         ggCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGa                                                                                                                          <  2:1129196/150‑1 (MQ=255)

                            cGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTa                                                                                                                       <  2:1476354/150‑1 (MQ=255)

                            cGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTa                                                                                                                       <  1:3461210/150‑1 (MQ=255)

                                   gTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTa                                                                                                                <  1:4711356/150‑1 (MQ=255)

                                     tCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGc                                                                                                              <  1:5427059/150‑1 (MQ=255)

                                     tCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGc                                                                                                              <  2:2848189/150‑1 (MQ=255)

                                         gcggcgCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACtt                                                                                                                                     >  1:3100589/1‑123 (MQ=255)

                                         gcggcgCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACtt                                                                                                                                     <  2:3100589/123‑1 (MQ=255)

                                          cggcgCCATGTTGGTCACGGGCCTGGCTTTCCTACCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTc                                                                                                         <  2:2616567/150‑1 (MQ=255)

                                          cggcgCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTc                                                                                                         <  2:5446802/150‑1 (MQ=255)

                                           ggcgCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCg                                                                                                        <  2:2733525/150‑1 (MQ=255)

                                            gcgcCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGt                                                                                                       <  1:1204594/150‑1 (MQ=255)

                                               ccATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCt                                                                                                    <  2:981905/150‑1 (MQ=255)

                                                  tGTTGGTCACGGGCGTGGCTTTCCTAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCT‑GCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGttt                                                                                                <  1:1315755/150‑1 (MQ=255)

                                                   gTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGttt                                                                                                >  2:4312022/1‑150 (MQ=255)

                                                     tGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCg                                                                                              <  1:4056805/150‑1 (MQ=255)

                                                        tCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTGTTTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGAc                                                                                            <  2:4372103/150‑1 (MQ=255)

                                                        tCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACg                                                                                           >  1:4592623/1‑150 (MQ=255)

                                                           cGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCt                                                                                        <  1:1438743/150‑1 (MQ=255)

                                                              gCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCtttt                                                                                     >  2:845977/1‑150 (MQ=255)

                                                               ccTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTc                                                                                    <  2:3295916/150‑1 (MQ=255)

                                                               ccTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTc                                                                                    <  1:3279458/150‑1 (MQ=255)

                                                                cTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCg                                                                                   >  2:2651781/1‑150 (MQ=255)

                                                                cTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCg                                                                                   >  1:4501304/1‑150 (MQ=255)

                                                                 tgtcTTTCCAAACGGGGTGCATTGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGc                                                                                  <  1:2600896/147‑1 (MQ=255)

                                                                  ggCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCt                                                                                 <  1:1329503/150‑1 (MQ=255)

                                                                   gCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTg                                                                                >  1:276419/1‑150 (MQ=255)

                                                                    cTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGc                                                                               <  1:2208231/150‑1 (MQ=255)

                                                                     tttCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTa                                                                                                                <  1:3394939/116‑1 (MQ=255)

                                                                     tttCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTa                                                                                                                >  2:3394938/1‑116 (MQ=255)

                                                                     tttCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTa                                                                                                                >  2:3394939/1‑116 (MQ=255)

                                                                     tttCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGcc                                                                              >  2:1699768/1‑150 (MQ=255)

                                                                      ttCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCa                                                                             <  2:4501304/150‑1 (MQ=255)

                                                                         cAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTg                                                                                                      >  2:2456786/1‑122 (MQ=255)

                                                                         cAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTg                                                                                                      <  1:2456786/122‑1 (MQ=255)

                                                                          aaaCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGcc                                                                         >  1:5036002/1‑150 (MQ=255)

                                                                          aaaCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGcc                                                                         >  1:5333128/1‑150 (MQ=255)

                                                                          aaaCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGcc                                                                         >  1:5338835/1‑150 (MQ=255)

                                                                          aaaCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGcc                                                                         >  1:1546605/1‑150 (MQ=255)

                                                                          aaaCGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGcc                                                                         >  1:1946987/1‑150 (MQ=255)

                                                                                    cAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGACAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTc                                                               >  2:3152344/1‑150 (MQ=255)

                                                                                      aTGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCaa                                                             >  1:1354784/1‑150 (MQ=255)

                                                                                       tGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCaaa                                                            <  1:195244/150‑1 (MQ=255)

                                                                                       tGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCaaa                                                            <  1:4312022/150‑1 (MQ=255)

                                                                                          tCGGATCGTG‑TTTATTACGGTGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaagga                                                         <  1:2130022/150‑1 (MQ=255)

                                                                                           cGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaaggaa                                                        <  1:1145721/150‑1 (MQ=255)

                                                                                            ggATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaaggaag                                                       <  2:2935555/150‑1 (MQ=255)

                                                                                             gATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaaggaagg                                                      >  1:4613072/1‑150 (MQ=255)

                                                                                             gATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAaaggaagg                                                      <  1:643197/150‑1 (MQ=255)

                                                                                               tCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAg                                                                                                                             >  2:4767512/1‑77 (MQ=255)

                                                                                               tCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAg                                                                                                                             <  1:4767512/77‑1 (MQ=255)

                                                                                                   g‑tTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGtt                                                >  1:4211581/1‑150 (MQ=255)

                                                                                                     ttttttACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTTGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTc                                               >  2:1974420/1‑150 (MQ=255)

                                                                                                     ttttttACGGGGTGTCGAAGTATTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTGTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTc                                               >  2:1645809/1‑150 (MQ=255)

                                                                                                        tttACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTTCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTg                                            <  2:2921331/150‑1 (MQ=255)

                                                                                                              gggTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTcc                                      >  1:4839686/1‑150 (MQ=255)

                                                                                                                   tCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGccc                                 >  1:62334/1‑150 (MQ=255)

                                                                                                                      aaGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGAt                              >  1:1279044/1‑150 (MQ=255)

                                                                                                                         ttttATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCtt                           >  1:1051454/1‑150 (MQ=255)

                                                                                                                         ttttATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCtt                           >  2:3875996/1‑150 (MQ=255)

                                                                                                                          tttATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCttt                          <  1:5042912/150‑1 (MQ=255)

                                                                                                                          tttATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCttt                          <  1:3159249/150‑1 (MQ=255)

                                                                                                                             aTGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCAc                       >  1:5110111/1‑150 (MQ=255)

                                                                                                                                 gTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGcc                   >  2:1735275/1‑150 (MQ=255)

                                                                                                                                 gTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGcc                   >  1:1713936/1‑150 (MQ=255)

                                                                                                                                 gTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGcc                   <  2:4900128/150‑1 (MQ=255)

                                                                                                                                    gACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAgg                >  1:883152/1‑150 (MQ=255)

                                                                                                                                     aCATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAggg               >  1:2798406/1‑150 (MQ=255)

                                                                                                                                        tatatCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATc            <  2:4839686/150‑1 (MQ=255)

                                                                                                                                        cagatCTGACGGAGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATc            >  1:3897862/4‑150 (MQ=255)

                                                                                                                                        cagatCTGACGGAGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATc            >  1:4892945/4‑150 (MQ=255)

                                                                                                                                            tCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAgg        <  2:5228223/150‑1 (MQ=255)

                                                                                                                                            tCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAgg        >  1:527359/1‑150 (MQ=255)

                                                                                                                                              tGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCg      >  1:3561686/1‑150 (MQ=255)

                                                                                                                                               gACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCgg     <  1:4135506/150‑1 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGAc                                                                                            <  1:1217046/60‑1 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGAc                                                                                            >  2:1217046/1‑60 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGACa  >  1:4963757/1‑150 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGACa  >  1:3028573/1‑150 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGACa  >  2:1362250/1‑150 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGACa  >  2:3258986/1‑150 (MQ=255)

                                                                                                                                                  tCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCAGACa  >  2:770064/1‑150 (MQ=255)

AGTTTGCTGGCTGGCCTGTTGTTGGGGCCGTGGATGTTCGTGCGGCGCCATGTTGGTCACGGGCCTGGCTTTCCAAACGGGGTGCAATGGTCGGATCGTG‑TTTTTTACGGGGTGTCGAAGTTTTATGCGTTGACATATATCTGACTCCGTCAGATCTGGCACTTGGCATCAGTGATTACCCATTAGCCATTCGTGCTGTTTCGACGTCTTTTCGCTGCCAGGCCTGCAGGCTTCAAAGGAAGGTGAGTTCCTGTAGTCCTGCCCGATCTTTCACTGCCAGGGATCAAGGCGGACA  >  NC_002947/2347144‑2347438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`