Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,458,962 G→T 5.0% R23L (CGC→CTC)  PP_2150 → thiamine biosynthesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,458,9620GT 5.0% 535.7 / 11.8 159R23L (CGC→CTC) PP_2150thiamine biosynthesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (99/52);  new base T (5/3);  total (104/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

CTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATC  >  NC_002947/2458815‑2459110
                                                                                                                                                   |                                                                                                                                                    
cTCCTACCGGTTTATCGCTGCCCCTGTTGGTGCGGTTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGcc                                                                                                                                                    <  1:481371/150‑1 (MQ=255)
  ccTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTg                                                                                                                                                  <  2:872789/150‑1 (MQ=255)
   cTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACGGTGCATTCTGCTCCTACTCACCGCCTGCACCCAAGGCCCCACCCTGGAGCGCCTgg                                                                                                                                                 >  1:2963764/1‑150 (MQ=255)
      cAGGATTAACGCGGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGGTCAGTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGggtg                                                                                                                                              <  1:4726240/150‑1 (MQ=255)
         gATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTc                                                                                                                                           >  2:714361/1‑150 (MQ=255)
            tATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGa                                                                                                                                        >  1:528394/1‑150 (MQ=255)
            tATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGa                                                                                                                                        >  1:24227/1‑150 (MQ=255)
             cgcgcgGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTCCTGTGCGCACAGCCCTCCTGTTCATTCTGCTGCTACTCCCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGAc                                                                                                                                       <  1:4071235/148‑1 (MQ=255)
             aTCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCAGGCGCTCCAGggtggggcctt                                                                                                                                       <  2:810542/150‑7 (MQ=39)
              tCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGAcc                                                                                                                                      <  2:24227/150‑1 (MQ=255)
                  tGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCTTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATgg                                                                                                                                  >  2:1629815/1‑150 (MQ=255)
                  tGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATgg                                                                                                                                  <  2:4462917/150‑1 (MQ=255)
                    ccccTGGAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgc                                                                                                                                <  2:2573991/150‑1 (MQ=255)
                     cccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgca                                                                                                                               >  2:3989038/1‑150 (MQ=255)
                      ccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcag                                                                                                                              >  2:1427427/1‑150 (MQ=255)
                      ccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCACCACCCAGGCGCTCCAGggtggggccttg                                                                                                                                      <  2:3609922/142‑8 (MQ=38)
                      ccTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCACCACCCAGGCGCTCCAGggtggggccttg                                                                                                                                      >  1:3609922/1‑135 (MQ=38)
                       cTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGgcagc                                                                                                                             >  2:312882/1‑150 (MQ=255)
                         gTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGcagcag                                                                                                                           >  1:604192/1‑150 (MQ=255)
                               gCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAg                                                                                                                     >  2:3421000/1‑150 (MQ=255)
                               gCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAg                                                                                                                     >  2:1198197/1‑150 (MQ=255)
                               gCGGGGTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAg                                                                                                                     >  2:361746/1‑150 (MQ=255)
                                   gTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                 >  2:3333505/1‑150 (MQ=255)
                                   gTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                 <  2:524992/150‑1 (MQ=255)
                                   gTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                 >  1:4826309/1‑150 (MQ=255)
                                   gTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATc                                                                                                                 <  1:3480223/150‑1 (MQ=255)
                                        cccGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTa                                                                                                            >  2:4188577/1‑150 (MQ=255)
                                                 agGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGa                                                                                                   <  2:3131750/150‑1 (MQ=255)
                                                      aGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtg                                                                                              <  2:682885/150‑1 (MQ=255)
                                                       gCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtgg                                                                                             <  1:1438522/150‑1 (MQ=255)
                                                         aCCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCtggtg                                                                                           <  1:2960886/150‑1 (MQ=255)
                                                           cGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGc                                                                                         >  2:2540919/1‑150 (MQ=255)
                                                               aaCCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGc                                                                                     >  2:4405959/1‑150 (MQ=255)
                                                                 ccGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTg                                                                                   >  1:3381337/1‑150 (MQ=255)
                                                                 ccGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTg                                                                                   >  2:4983327/1‑150 (MQ=255)
                                                                  cGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGc                                                                                  >  1:5029353/1‑150 (MQ=255)
                                                                          ccTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAg                                                                          <  1:1166294/150‑1 (MQ=255)
                                                                           cTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGa                                                                                                   <  2:1848819/124‑1 (MQ=255)
                                                                           cTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGa                                                                                                   >  1:1848819/1‑124 (MQ=255)
                                                                           cTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGCCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAgg                                                                         >  1:1969925/1‑150 (MQ=255)
                                                                             aCTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTg                                                                       <  1:1629815/150‑1 (MQ=255)
                                                                              cTGCGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGc                                                                      <  1:2650856/150‑1 (MQ=255)
                                                                              cTGCGCGCACAGCCCTACTGTTCATTCTGCTGCGACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGc                                                                      <  1:2650874/150‑1 (MQ=255)
                                                                                 tgCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGATCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGa                                                                   >  2:5393472/1‑150 (MQ=255)
                                                                                    tcacaGCCATAATCTTCATTCTCCTGCTACTCACCGCCTGCAACCAAGGCACCAACAAGGAGCGCCTGGGTGGTCCGACCTTGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATgg                                                                >  2:2744478/2‑150 (MQ=255)
                                                                                    gcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCTTGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATgg                                                                >  2:2744729/1‑150 (MQ=255)
                                                                                    gcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATgg                                                                >  1:14985/1‑150 (MQ=255)
                                                                                    gcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATgg                                                                >  1:4762427/1‑150 (MQ=255)
                                                                                    gcACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATgg                                                                >  1:14994/1‑150 (MQ=255)
                                                                                      acaGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCg                                                              >  1:5365329/1‑150 (MQ=255)
                                                                                            cTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaa                                                        >  2:5194791/1‑150 (MQ=255)
                                                                                             tACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGaaa                                                       >  2:394231/1‑150 (MQ=255)
                                                                                                 gTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCAt                                                   >  2:4412530/1‑150 (MQ=255)
                                                                                                  ttCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                  >  1:183638/1‑150 (MQ=255)
                                                                                                  ttCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                  >  2:2009768/1‑150 (MQ=255)
                                                                                                  ttCATTCTGCTGATACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATc                                                  >  1:3073465/1‑150 (MQ=255)
                                                                                                   tCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTgg                                                          >  1:598975/1‑141 (MQ=255)
                                                                                                   tCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATcc                                                 <  2:5337034/150‑1 (MQ=255)
                                                                                                       tCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCa                                             <  1:2275848/150‑1 (MQ=255)
                                                                                                        ctgctgCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCAc                                            <  1:4027871/150‑1 (MQ=255)
                                                                                                         tgctgcTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACg                                           >  2:1505301/1‑150 (MQ=255)
                                                                                                           ctgctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGAc                                         >  1:1182391/1‑150 (MQ=255)
                                                                                                             gctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAt                                       <  1:669823/150‑1 (MQ=255)
                                                                                                             gctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAt                                       >  2:2627232/1‑150 (MQ=255)
                                                                                                             gctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACAt                                       >  2:5148159/1‑150 (MQ=255)
                                                                                                              ctACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATc                                      <  2:1305176/150‑1 (MQ=255)
                                                                                                                 cTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGac                                   <  2:4802346/150‑1 (MQ=255)
                                                                                                                  tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCg                                     <  1:1942968/147‑1 (MQ=255)
                                                                                                                  tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCg                                     >  2:1942968/1‑147 (MQ=255)
                                                                                                                  tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacc                                  >  2:2898251/1‑150 (MQ=255)
                                                                                                                  tCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacc                                  >  1:4975061/1‑150 (MQ=255)
                                                                                                                   cACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGacca                                 >  1:249021/1‑150 (MQ=255)
                                                                                                                    aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGaccac                                >  1:3105399/1‑150 (MQ=255)
                                                                                                                    aCCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGaccac                                >  2:2046465/1‑150 (MQ=255)
                                                                                                                      cGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGAccacca                              >  1:2009741/1‑150 (MQ=255)
                                                                                                                      cGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGAccacca                              >  1:2009819/1‑150 (MQ=255)
                                                                                                                       gCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACcaccac                             >  2:1308039/1‑150 (MQ=255)
                                                                                                                          tGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCactac                          >  2:543953/1‑150 (MQ=255)
                                                                                                                          tGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCactac                          >  2:4161681/1‑150 (MQ=255)
                                                                                                                          tGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCactac                          >  1:3756610/1‑150 (MQ=255)
                                                                                                                          tGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCactac                          >  1:3415887/1‑150 (MQ=255)
                                                                                                                          tGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCactac                          >  2:3314892/1‑150 (MQ=255)
                                                                                                                            cAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTc                        <  2:502427/150‑1 (MQ=255)
                                                                                                                             aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCg                       >  1:116281/1‑150 (MQ=255)
                                                                                                                             aaCCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCg                       >  2:2439608/1‑150 (MQ=255)
                                                                                                                               ccAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGAc                     >  1:4244602/1‑150 (MQ=255)
                                                                                                                                 aaGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCt                   <  2:604192/150‑1 (MQ=255)
                                                                                                                                 aaGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCt                   >  1:896490/1‑150 (MQ=255)
                                                                                                                                    ggaccACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTAcc                >  1:3225645/4‑150 (MQ=255)
                                                                                                                                       ccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcgcg             >  1:4489668/1‑150 (MQ=255)
                                                                                                                                       ccACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACcgcg             <  1:1015622/150‑1 (MQ=255)
                                                                                                                                        cACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCgcgg            <  1:4136846/150‑1 (MQ=255)
                                                                                                                                        cACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCgcgg            >  1:4568609/1‑150 (MQ=255)
                                                                                                                                        cACCCAGGCGCTCCAGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCgcgg            >  1:2698071/1‑150 (MQ=255)
                                                                                                                                           ccTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGa         >  2:1863057/1‑150 (MQ=255)
                                                                                                                                            cTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGAt        <  2:4070224/150‑1 (MQ=255)
                                                                                                                                              ggAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTc      >  1:4031693/1‑150 (MQ=255)
                                                                                                                                                aGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCa    <  2:4826309/150‑1 (MQ=255)
                                                                                                                                                aGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCa    <  2:2176867/150‑1 (MQ=255)
                                                                                                                                                  cgcCTGGGTGGCCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATc  <  2:1969925/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTCCTACAGGATTATCGCTGCCCCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCAGCACCGGCAACCGAGAAACCCTACTGTGCGCACAGCCCTACTGTTCATTCTGCTGCTACTCACCGCCTGCAACCAAGGCCCCACCCTGGAGCGCCTGGGTGGTCCGACCATGGGCAGCAGCTACAGCATCCAGTACGTCCGCGAGCCTGGTGGCCCGCTGCCGGCCCAGGTGCAGATGGCGGTGGAAACCATCCTGCACGACATCGACCACCACTACTCGACCTACCGCGGCGATTCCATC  >  NC_002947/2458815‑2459110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑