BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,482,754	G→A	5.2%	T186T (ACC→ACT)	PP_2175 ←	3‑oxoacyl‑ACP reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,482,754	0	G	A	5.2%	658.5 / 16.6	209	T186T (ACC→ACT)	PP_2175	3‑oxoacyl‑ACP reductase
Reads supporting (aligned to +/- strand): ref base G (97/101); new base A (5/6); total (102/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

Only 100 of 211 total aligned reads displayed.

AATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACG  >  NC_002947/2482605‑2482902

aaTCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCg                                                                                                                                                      >  2:1014039/1‑150 (MQ=255)

  tCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGt                                                                                                                                                    >  1:750502/1‑150 (MQ=255)

   cacaCGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtg                                                                                                                                                   <  2:2549140/147‑1 (MQ=255)

   cAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtg                                                                                                                                                   <  1:2202008/150‑1 (MQ=255)

    aaaCGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtgg                                                                                                                                                  >  2:4639941/1‑150 (MQ=255)

     accGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtggc                                                                                                                                                 <  2:2151772/148‑1 (MQ=255)

     aaCGCTGCTTGTCAAGCCGCCATAGACCTTGATGTTCTGCACGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGAATTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGtggc                                                                                                                                                 >  2:1656876/1‑150 (MQ=255)

            cGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAgg                                                                                                                                          <  1:1013600/150‑1 (MQ=255)

               gtCAAGTCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTg                                                                                                                                       <  1:210932/150‑1 (MQ=255)

                 cAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTc                                                                                                                                     <  2:6261/150‑1 (MQ=255)

                  aaGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCg                                                                                                                                    >  2:3033613/1‑150 (MQ=255)

                    gccgccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGAt                                                                                                                                  <  2:3951450/150‑1 (MQ=255)

                     ccgccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATc                                                                                                                                 <  2:887726/150‑1 (MQ=255)

                        ccATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAg                                                                                                                              >  1:1862020/1‑150 (MQ=255)

                          aTCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGcc                                                                                                                            >  2:5076203/1‑150 (MQ=255)

                          aTCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGcc                                                                                                                            >  2:1589438/1‑150 (MQ=255)

                            cGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCgg                                                                                                                          <  2:4092229/150‑1 (MQ=255)

                             gACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCggg                                                                                                                         >  1:4788625/1‑150 (MQ=255)

                               ccTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGgga                                                                                                                       <  2:5326089/150‑1 (MQ=255)

                               ccTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGgga                                                                                                                       <  1:3435611/150‑1 (MQ=255)

                                 ttGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGggagg                                                                                                                     <  1:3224999/150‑1 (MQ=255)

                                  tGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGggagga                                                                                                                    >  2:2001780/1‑150 (MQ=255)

                                   gATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGAc                                                                                                                   >  1:3103188/1‑150 (MQ=255)

                                      ggtCTGCCCGGGGCTGTCGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgtt                                                                                                                <  1:4071078/148‑1 (MQ=255)

                                        tCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgt                                                                                                              <  2:2240475/150‑1 (MQ=255)

                                        tCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgt                                                                                                              >  2:3719194/1‑150 (MQ=255)

                                         cTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgtt                                                                                                             <  1:3139740/150‑1 (MQ=255)

                                         cTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACgttgtt                                                                                                             <  2:1959085/150‑1 (MQ=255)

                                           gCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGa                                                                                                           <  2:4394386/150‑1 (MQ=255)

                                           gCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGa                                                                                                           >  1:1327087/1‑150 (MQ=255)

                                            cccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGAt                                                                                                          >  1:498223/1‑150 (MQ=255)

                                            cccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGAt                                                                                                          >  1:2005834/1‑150 (MQ=255)

                                            cccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGAt                                                                                                          >  1:37518/1‑150 (MQ=255)

                                            acctgTGATGTAGGCCGCACCGTCTGATGCCAGGAAGGCAACTGTTGCGGCAATTTCTTCGCTTTTGCCATAACGCTGCATAGGCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCAt                                                                                                          >  2:2457568/5‑150 (MQ=255)

                                             ccGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATc                                                                                                         <  2:321367/150‑1 (MQ=255)

                                               tgTGATGTAGGCCGCACCGTCTGATGCCAGGAAGGCAACTGTTGCGGCAATTTCTTCGCTTTTGCCATAACGCTGCATAGGCACGCTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCAtacg                                                                                                       >  2:4700163/2‑147 (MQ=255)

                                                gTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTc                                                                                                      >  2:385936/1‑150 (MQ=255)

                                                 tGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCa                                                                                                     <  2:4112744/150‑1 (MQ=255)

                                                     gTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                                 <  1:3551224/150‑1 (MQ=255)

                                                     gTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGtt                                                                                                 >  1:5123981/1‑150 (MQ=255)

                                                         gCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcc                                                                                             >  2:3063703/1‑150 (MQ=255)

                                                         gCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTcc                                                                                             >  2:2362044/1‑150 (MQ=255)

                                                          cAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTccc                                                                                            <  2:5258216/150‑1 (MQ=255)

                                                          cAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTccc                                                                                            <  1:4729512/150‑1 (MQ=255)

                                                          cAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTccc                                                                                            <  2:3978691/150‑1 (MQ=255)

                                                          cAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTccc                                                                                            >  2:4452153/1‑150 (MQ=255)

                                                            gcgcCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCg                                                                                          >  2:1888124/1‑150 (MQ=255)

                                                              gcCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATCGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGc                                                                                        >  2:4047816/1‑150 (MQ=255)

                                                                cTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCGCGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGa                                                                                      >  1:1364736/1‑150 (MQ=255)

                                                                 ttCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGaa                                                                                     >  2:1507173/1‑150 (MQ=255)

                                                                 ttCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGaa                                                                                     <  2:1326100/150‑1 (MQ=255)

                                                                  tCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAAt                                                                                    <  1:2541027/150‑1 (MQ=255)

                                                                  tCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAAt                                                                                    >  2:2541027/1‑150 (MQ=255)

                                                                      tGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGt                                                                                >  1:3641197/1‑150 (MQ=255)

                                                                       ggCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTc                                                                               <  2:3196759/150‑1 (MQ=255)

                                                                          gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCTGGGAGGACTTTGTTGATCCTCACGTTGTCCCCTGCGAATTGGTCGGc                                                                            <  2:5013131/150‑1 (MQ=255)

                                                                          gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGc                                                                            >  1:1079273/1‑150 (MQ=255)

                                                                          gagaAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGc                                                                            <  1:1780393/150‑1 (MQ=255)

                                                                             aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGt                                                                                                                                      >  2:1429176/1‑89 (MQ=255)

                                                                             aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGt                                                                                                                                      <  1:1429176/89‑1 (MQ=255)

                                                                             aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                                         <  1:2326796/150‑1 (MQ=255)

                                                                             aaaCGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGaa                                                                         >  2:2789525/1‑150 (MQ=255)

                                                                                cGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCa                                                                                                     <  1:1292661/119‑1 (MQ=255)

                                                                                cGCAATCGTGGCGGCGATTTCCTCGCAGGAGCCATAGCCATTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGACGAACCAGCCGGGGAGGACGTTGTTCATCCTCACGTTGTCCCCGGCGAATAGGTCGGCGAAGAt                                                                      >  2:4505394/1‑150 (MQ=255)

                                                                                    aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                  <  1:4381133/150‑1 (MQ=255)

                                                                                    aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                  <  1:2135747/150‑1 (MQ=255)

                                                                                    aTGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTg                                                                  <  1:3906160/150‑1 (MQ=255)

                                                                                      ggtggCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGt                                                                <  2:4249756/150‑1 (MQ=255)

                                                                                            ggcgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGc                                                          >  2:3758442/1‑150 (MQ=255)

                                                                                              cgATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCgg                                                        <  2:4597581/150‑1 (MQ=255)

                                                                                               gATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGCCGATTTTGGTGAATGCGGc                                                       >  2:4888572/1‑150 (MQ=255)

                                                                                               gATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGc                                                       <  1:1382242/150‑1 (MQ=255)

                                                                                               gATTTCCTCGCAGGTGCCATAGCGTATGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCAGTCGATCCAGCCGGGCAGGACGATGAAGATCATCACGTTCTCCCCGGCGAATAGGTCGGCGAAGATTTTGGTGAATCCGGc                                                       >  2:694779/1‑150 (MQ=255)

                                                                                                     cTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGcc                                                 >  1:4159149/1‑150 (MQ=255)

                                                                                                       cGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCg                                               >  1:4623080/1‑150 (MQ=255)

                                                                                                               ccATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAac                                       >  1:5159882/1‑150 (MQ=255)

                                                                                                               ccATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAac                                       >  1:2782827/1‑150 (MQ=255)

                                                                                                               ccATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAac                                       >  1:222329/1‑150 (MQ=255)

                                                                                                                 aTAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATg                                                           >  1:4013776/1‑128 (MQ=255)

                                                                                                                  tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                    <  2:4057237/150‑1 (MQ=255)

                                                                                                                  tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                    >  2:4366220/1‑150 (MQ=255)

                                                                                                                  tAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACt                                    >  2:3458642/1‑150 (MQ=255)

                                                                                                                  ccgCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTAGATCCTCACGCGGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCACGAGCGGAAAACt                                    <  2:3919876/148‑1 (MQ=255)

                                                                                                                     cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACCGCa                                 <  2:2735233/150‑1 (MQ=255)

                                                                                                                     cGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGAGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCa                                 <  1:5319724/150‑1 (MQ=255)

                                                                                                                           aGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTc                           >  1:444034/1‑150 (MQ=255)

                                                                                                                            gCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCg                          <  1:4639941/150‑1 (MQ=255)

                                                                                                                             cGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCgg                         >  2:2609394/1‑150 (MQ=255)

                                                                                                                                cACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGTCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAATATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGaa                      <  1:1656876/150‑1 (MQ=255)

                                                                                                                                  cGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACa                    <  2:870081/150‑1 (MQ=255)

                                                                                                                                    cTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACatt                  <  1:4834138/150‑3 (MQ=255)

                                                                                                                                    cTGTCACGGCGCTCTTCAGTGGCTGGCAGGCTGTCGATCCAGCCCGGCAAAACATTGTTCATGCGAATGTTGTCGGGTGCATAAGTGTCGGCAAATACCTTCGTGAAAGAGGCGAGGCCTGCGCGGAACACCGCCGAAGTCGGGAACatt                  <  1:4676905/150‑3 (MQ=255)

                                                                                                                                     tGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGtt                 >  2:3883402/1‑150 (MQ=255)

                                                                                                                                       tCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCa               >  2:398822/1‑150 (MQ=255)

                                                                                                                                          cggcgCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTTATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTg            <  1:435182/150‑1 (MQ=255)

                                                                                                                                               cTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTc       <  2:1235376/150‑1 (MQ=255)

                                                                                                                                                 gTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAg                                             >  2:2676409/1‑110 (MQ=255)

                                                                                                                                                 gTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAg                                             <  1:2676409/110‑1 (MQ=255)

                                                                                                                                                   tCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTAGAAc   <  1:1128737/150‑1 (MQ=255)

                                                                                                                                                    cGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACg  >  1:611129/1‑150 (MQ=255)

AATCAAACGCTGCGTGTCAAGCCGCCATCGACCTTGATGTTCTGCCCGGTGATGTAGGCAGCGCCTTCGCTGGCGAGAAACGCAATGGTGGCGGCGATTTCCTCGCAGGTGCCATAGCGTTTGAGCGGCACGCTGTCGCGGCGCTGTTCGGTGGCTGGCAGGCTGTCGATCCAGCCGGGGAGGACGTTGTTGATCCTCACGTTGTCCCCGGCGAATTGGTCGGCGAAGATTTTGGTGAATGCGGCCAGGCCCGAGCGGAACACTGCAGACGTCGGGAACAGTTCACTGGGCTCGAACG  >  NC_002947/2482605‑2482902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`