BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,504,956	A→G	5.6%	T96A (ACG→GCG) ‡	speB →	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,504,956	0	A	G	5.6%	621.8 / 15.6	195	T96A (ACG→GCG) ‡	speB	agmatinase
Reads supporting (aligned to +/- strand): ref base A (83/101); new base G (7/4); total (90/105)
Fisher's exact test for biased strand distribution p-value = 3.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 198 total aligned reads displayed.

CCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGAT  >  NC_002947/2504807‑2505104

ccaGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCa                                                                                                                                                      <  2:2878835/150‑1 (MQ=255)

   gTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgc                                                                                                                                                   <  1:5178654/150‑1 (MQ=255)

   gTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgc                                                                                                                                                   >  2:1977200/1‑150 (MQ=255)

    tAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgcc                                                                                                                                                  <  1:5143354/150‑1 (MQ=255)

    tAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgcc                                                                                                                                                  >  2:4070323/1‑150 (MQ=255)

     aaCCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgccg                                                                                                                                                 <  2:5143640/150‑1 (MQ=255)

       ccGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCa                                                                                                                                               <  1:1693792/150‑1 (MQ=255)

       ccGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCCGTCCAGCAGGCCTGCGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCCACTAAGGCGACTGACCCTTCGACAGCGGCACGCCGCa                                                                                                                                               >  2:3063627/1‑150 (MQ=255)

        cGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAg                                                                                                                                              >  1:3008816/1‑150 (MQ=255)

         gCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGt                                                                                                                                             <  2:4796950/150‑1 (MQ=255)

         gCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGt                                                                                                                                             >  1:5268961/1‑150 (MQ=255)

          ccccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCCGGCCTGGGCCCGCCACTGGCCGTGGGCGGTCGACCCGATCGACCACCTGGCGGTCATCGACTCTGGCGACTGTCCCGTCGACAGCGGCACGCCGCAGTc                                                                                                                                            >  2:3331423/1‑150 (MQ=255)

          ccccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTc                                                                                                                                            <  2:3477704/150‑1 (MQ=255)

           cccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCg                                                                                                                                           <  2:779817/150‑1 (MQ=255)

           cccGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCg                                                                                                                                           >  2:4312744/1‑150 (MQ=255)

             cGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACTGCGGCGTGCCGCTGTCgaa                                                                                                                                         <  2:2365619/150‑3 (MQ=255)

                 gcgcgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTTGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCg                                                                                                                                     <  2:2579158/150‑1 (MQ=255)

                 gcgcgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCg                                                                                                                                     <  2:4143812/150‑1 (MQ=255)

                   gcgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGa                                                                                                                                   <  1:4320738/150‑1 (MQ=255)

                    cgcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGAc                                                                                                                                  <  1:2796928/150‑1 (MQ=255)

                     gcTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACa                                                                                                                                 <  1:1524042/150‑1 (MQ=255)

                           gCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTTGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCg                                                                                                                           >  2:4024247/1‑150 (MQ=255)

                               gcgGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGc                                                                                                                       <  2:446903/150‑1 (MQ=255)

                                 gggCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATc                                                                                                                            >  1:1108933/1‑143 (MQ=255)

                                 gggCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATc                                                                                                                            <  2:1108933/143‑1 (MQ=255)

                                 gggCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATc                                                                                                                            >  1:3844160/1‑143 (MQ=255)

                                   gCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgccg                                                                                                                                                 <  2:1806934/120‑1 (MQ=255)

                                   gCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCAcgccg                                                                                                                                                 >  1:1806934/1‑120 (MQ=255)

                                     gATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGc                                                                                                                 <  1:3416877/150‑1 (MQ=255)

                                      aTCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGcc                                                                                                                <  2:4756886/150‑1 (MQ=255)

                                        ccGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGa                                                                                                              <  1:4070323/150‑1 (MQ=255)

                                        ccGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGa                                                                                                              <  1:2384465/150‑1 (MQ=255)

                                          gTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGc                                                                                                            >  1:2485260/1‑150 (MQ=255)

                                            gctgcCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGcac                                                                                                          >  2:3952852/1‑150 (MQ=255)

                                               gcCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACAtt                                                                                                       >  1:697065/1‑150 (MQ=255)

                                                ccTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTc                                                                                                      <  2:894962/150‑1 (MQ=255)

                                                ccTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTc                                                                                                      >  1:5208632/1‑150 (MQ=255)

                                                 cTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCt                                                                                                     <  2:697065/150‑1 (MQ=255)

                                                  tCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTg                                                                                                    <  2:731740/150‑1 (MQ=255)

                                                  tCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGGTCGACAACCTGTCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTg                                                                                                    >  1:2210255/1‑150 (MQ=255)

                                                    ggTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCa                                                                                                  <  2:2532380/150‑1 (MQ=255)

                                                        cagcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCg                                                                                              <  2:3072235/150‑1 (MQ=255)

                                                        cagcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCg                                                                                              <  2:5005762/150‑1 (MQ=255)

                                                         agcagGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCgg                                                                                             <  1:1256357/150‑1 (MQ=255)

                                                              gCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTgc                                                                                        <  1:1318594/150‑1 (MQ=255)

                                                                   ggCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCAt                                                                                   <  2:2001779/150‑1 (MQ=255)

                                                                        gCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGa                                                                              <  1:4659407/150‑1 (MQ=255)

                                                                          cACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGAcc                                                                            >  2:3665450/1‑150 (MQ=255)

                                                                             tGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCtt                                                                         >  1:595242/1‑150 (MQ=255)

                                                                             tGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCtt                                                                         >  1:595406/1‑150 (MQ=255)

                                                                               gCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgg                                                                       >  2:2615025/1‑150 (MQ=255)

                                                                                ccGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtggt                                                                      <  2:3148644/150‑1 (MQ=255)

                                                                                ccGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtggt                                                                      <  1:4155182/150‑1 (MQ=255)

                                                                                 cGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtggtg                                                                     >  1:1385358/1‑150 (MQ=255)

                                                                                 cGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCAACGACTATGGCGACTGTCCCAACGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCAAGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTtgaga                                                                     >  1:2534944/1‑147 (MQ=255)

                                                                                   tGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGc                                                                   <  1:1719424/150‑1 (MQ=255)

                                                                                   tGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGc                                                                   <  2:1669196/150‑1 (MQ=255)

                                                                                   tGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGc                                                                   >  2:1097733/1‑150 (MQ=255)

                                                                                   tGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGGACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGc                                                                   <  2:2039043/150‑1 (MQ=255)

                                                                                    gggCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCg                                                                  <  2:3404342/150‑1 (MQ=255)

                                                                                     ggCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGa                                                                 <  2:1389875/150‑1 (MQ=255)

                                                                                     ggCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGCCGACTCTCCCTTCTACAGAGGCACGCCGCAGTCGGTACCGGACAGCATCGAGCCCCAACCCGAGCACATTCTGCAGGCGGGATGCGCCAAGCTGACCCTTGGTGGCGa                                                                 >  2:3262058/1‑150 (MQ=255)

                                                                                        gttcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCa                                                              >  2:854872/1‑150 (MQ=255)

                                                                                          tcgacccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACt                                                            <  2:1486641/150‑1 (MQ=255)

                                                                                              cccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcat                                                        <  2:4949950/150‑1 (MQ=255)

                                                                                              cccgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcat                                                        <  1:1259569/150‑1 (MQ=255)

                                                                                                cgttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTtcatca                                                      <  1:5385233/150‑1 (MQ=255)

                                                                                                 gttcgaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAg                                                     >  2:4398648/1‑150 (MQ=255)

                                                                                                     gaccACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTAc                                                 <  1:3033364/150‑1 (MQ=255)

                                                                                                           cTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCgctg                                           <  1:4818958/150‑1 (MQ=255)

                                                                                                             ggCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCgctgct                                         <  1:3631558/150‑1 (MQ=255)

                                                                                                                 gTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAg                                     <  1:1977200/150‑1 (MQ=255)

                                                                                                                 gTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAg                                     <  1:3665450/150‑1 (MQ=255)

                                                                                                                   cATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGc                                   >  2:1929884/1‑150 (MQ=255)

                                                                                                                    aTCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCt                                  >  1:4548289/1‑150 (MQ=255)

                                                                                                                    aTCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCt                                  >  1:26981/1‑150 (MQ=255)

                                                                                                                     tCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTc                                 >  1:2678855/1‑150 (MQ=255)

                                                                                                                      cGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCa                                >  1:5264468/1‑150 (MQ=255)

                                                                                                                         cTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcgc                             >  1:5147932/1‑150 (MQ=255)

                                                                                                                           aTGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCAcgccc                           >  2:3547109/1‑150 (MQ=255)

                                                                                                                             ggCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCt                                                                                                     >  2:3831724/1‑74 (MQ=255)

                                                                                                                               cGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCccgccg                       >  1:325301/1‑150 (MQ=255)

                                                                                                                                gACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCcgccgc                      >  2:4206641/1‑150 (MQ=255)

                                                                                                                                gACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCcgccgc                      >  2:531217/1‑150 (MQ=255)

                                                                                                                                    gTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATg                  <  2:115099/150‑1 (MQ=255)

                                                                                                                                      ggtaccGACTGCGGCGTGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGc                >  1:4467177/6‑150 (MQ=255)

                                                                                                                                       ccTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGcc               >  1:2887172/1‑150 (MQ=255)

                                                                                                                                        cTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCATGCCCGCCGCCATGGccc              >  1:3076659/1‑150 (MQ=255)

                                                                                                                                        cTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGccc              >  1:3784623/1‑150 (MQ=255)

                                                                                                                                          ccGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGc            >  2:1043225/2‑150 (MQ=255)

                                                                                                                                           cGACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCt           >  2:2778301/1‑150 (MQ=255)

                                                                                                                                            gACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGa                                                                              >  1:2164976/1‑82 (MQ=255)

                                                                                                                                            gACTGCGGCGTGCCGCTGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGa                                                                              <  2:2164976/82‑1 (MQ=255)

                                                                                                                                             aCAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTgg         >  2:3938864/1‑150 (MQ=255)

                                                                                                                                             aCAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCGTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTgg         >  2:559274/1‑150 (MQ=255)

                                                                                                                                              cAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgc        <  2:2693890/150‑1 (MQ=255)

                                                                                                                                              cAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgc        <  1:563679/150‑1 (MQ=255)

                                                                                                                                               aGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgcg       >  1:815814/1‑150 (MQ=255)

                                                                                                                                                gCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGgcgc      <  1:854872/150‑1 (MQ=255)

                                                                                                                                                    cACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGAt  >  2:3492512/1‑150 (MQ=255)

CCAGTAACCGCCCCGGCGCGCGCTTCGGCCCGCGGGCGATCCGTGCTGCCTCGGTCCAGCAGGCCTGGGCCCGCCACTGGCCGTGGGCGTTCGACCCGTTCGACCACCTGGCGGTCATCGACTATGGCGACTGTCCCTTCGACAGCGGCACGCCGCAGTCGGTACCGGACAGCATCGAGGCCCATGCCGAGCACATTCTGCAGGCGGGTTGCGCCATGCTGACCCTTGGTGGCGACCACTTCATCAGCTACCCGCTGCTCAAGGCTCACGCCCGCCGCCATGGCCCGCTGGCGCTGAT  >  NC_002947/2504807‑2505104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`