BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,562,429	G→C	6.3%	W142C (TGG→TGC) ‡	PP_2243 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,562,429	0	G	C	6.3%	528.9 / 19.1	159	W142C (TGG→TGC) ‡	PP_2243	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (69/80); new base C (4/6); total (73/86)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 166 total aligned reads displayed.

ATTGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTTTAC  >  NC_002947/2562281‑2562578

aTTGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACACGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCctggc                                                                                                                                                      <  2:4532024/150‑1 (MQ=255)

  tGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCctggctg                                                                                                                                                    >  2:830480/1‑150 (MQ=255)

  tGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCctggctg                                                                                                                                                    >  1:4573872/1‑150 (MQ=255)

       ccTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACATGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCTGGATCCGGCCAGTGCCGTGGATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCGAGGCCGCGTGGCTggcggc                                                                                                                                               <  1:1165236/150‑1 (MQ=255)

        cTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTggcgctg                                                                                                                                              <  2:4695389/150‑4 (MQ=255)

        cTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGcc                                                                                                                                              >  2:193240/1‑150 (MQ=255)

         ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCt                                                                                                                                             >  1:3968274/1‑150 (MQ=255)

         ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCGGGCGGCCt                                                                                                                                             >  2:1031210/1‑150 (MQ=255)

          tGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCtt                                                                                                                                            <  2:3983062/150‑1 (MQ=255)

          tGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtg                                                                                                                                            <  1:4964430/150‑2 (MQ=255)

            ggCGTTGGCAAGGTGTGTCGTTGAAGCAACTACCAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCg                                                                                                                                          <  2:2560185/150‑1 (MQ=255)

              cGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGCCtggcgc                                                                                                                                        <  2:1694923/150‑6 (MQ=255)

              cGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGCCtggcgc                                                                                                                                        <  2:1695093/150‑6 (MQ=255)

               ggtGGCAAGGTGTGTCGTTGAAGCAACTACACGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACa                                                                                                                                       <  1:1897967/148‑1 (MQ=255)

               gTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACa                                                                                                                                       <  1:560414/150‑1 (MQ=255)

                ttGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCtgtcgaag                                                                                                                                      <  1:3646932/150‑8 (MQ=255)

                     aaGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCgg                                                                                                                                 >  2:4123546/1‑150 (MQ=255)

                     aaGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCgg                                                                                                                                 <  2:3776606/150‑1 (MQ=255)

                         tgtgtCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGa                                                                                                                             <  1:5064254/150‑1 (MQ=255)

                             tCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActgg                                                                                                                         <  1:105087/150‑1 (MQ=255)

                               gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggct                                                                                                                       >  1:95224/1‑150 (MQ=255)

                               gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggct                                                                                                                       >  1:2809263/1‑150 (MQ=255)

                               gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggct                                                                                                                       >  2:1620938/1‑150 (MQ=255)

                               gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggct                                                                                                                       >  2:1031358/1‑150 (MQ=255)

                                ttGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggctg                                                                                                                      >  2:3262051/1‑150 (MQ=255)

                                   aaGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCg                                                                                                                   <  2:1947731/150‑1 (MQ=255)

                                     gCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGt                                                                                                                 >  1:3105407/1‑150 (MQ=255)

                                       aaCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGGGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGa                                                                                                               >  1:5421681/1‑150 (MQ=255)

                                       aaCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGa                                                                                                               <  1:4323029/150‑1 (MQ=255)

                                       aaCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGa                                                                                                               <  1:4375243/150‑1 (MQ=255)

                                         cTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGacc                                                                                                             <  1:637337/150‑1 (MQ=255)

                                          tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                            <  1:5419021/150‑1 (MQ=255)

                                          tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                            <  1:4509005/150‑1 (MQ=255)

                                          tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                            >  1:5197700/1‑150 (MQ=255)

                                          tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                            <  1:2382302/150‑1 (MQ=255)

                                          tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                            <  1:4508887/150‑1 (MQ=255)

                                           aCAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccca                                                                                                           >  2:491821/1‑150 (MQ=255)

                                             aaGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGacccacc                                                                                                         >  2:3112906/1‑150 (MQ=255)

                                               gCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCg                                                                                                       >  1:903338/1‑150 (MQ=255)

                                               gCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCg                                                                                                       >  2:3418103/1‑150 (MQ=255)

                                                  gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                    <  1:846700/150‑1 (MQ=255)

                                                  gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                    <  1:257128/150‑1 (MQ=255)

                                                   aGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATgg                                                                                                   <  1:2679215/150‑1 (MQ=255)

                                                     cAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTc                                                                                                 >  2:1302210/1‑150 (MQ=255)

                                                       aCCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                               <  2:3331942/150‑1 (MQ=255)

                                                                gTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCAt                                                                                      <  1:597652/150‑1 (MQ=255)

                                                                  tGGCCGAATGGCTGCAGGATCCGGCCAGCGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATg                                                                                              >  2:5388317/1‑140 (MQ=255)

                                                                  tGGCCGAATGGCTGCAGGATCCGGCCAGCGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATg                                                                                              <  1:5388317/140‑1 (MQ=255)

                                                                   ggCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCt                                                                                   >  2:1850552/1‑150 (MQ=255)

                                                                      cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGac                                                                                                              <  1:4828597/120‑1 (MQ=255)

                                                                      cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                                <  2:5169835/150‑1 (MQ=255)

                                                                      cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                                >  2:292739/1‑150 (MQ=255)

                                                                       gAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGtc                                                                               >  2:4363967/1‑149 (MQ=255)

                                                                         aTGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACa                                                                             <  1:3792979/150‑1 (MQ=255)

                                                                          tGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAg                                                                            <  2:476951/150‑1 (MQ=255)

                                                                          tGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAg                                                                            <  2:2345197/150‑1 (MQ=255)

                                                                              tGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGc                                                                        >  1:1861487/1‑150 (MQ=255)

                                                                                cAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCtt                                                                      <  1:491821/150‑1 (MQ=255)

                                                                                cAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCtt                                                                      <  1:1363047/150‑1 (MQ=255)

                                                                                 aGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTg                                                                     >  2:5027773/1‑150 (MQ=255)

                                                                                  ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                    <  1:4224131/150‑1 (MQ=255)

                                                                                  ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                    <  1:1845282/150‑1 (MQ=255)

                                                                                  ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                    <  2:742809/150‑1 (MQ=255)

                                                                                  ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                    <  1:3365208/150‑1 (MQ=255)

                                                                                        ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                              >  1:1433911/1‑150 (MQ=255)

                                                                                        ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                              >  1:1433723/1‑150 (MQ=255)

                                                                                        ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                              <  1:3622661/150‑1 (MQ=255)

                                                                                        ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                              >  1:301302/1‑150 (MQ=255)

                                                                                           cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTGGACAGGCCGGGTGACTGGGTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGGTTGGGTGTCCgtt                                                           >  1:1428195/1‑148 (MQ=255)

                                                                                           cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGAt                                                           <  2:7722/150‑1 (MQ=255)

                                                                                           cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGAt                                                           <  2:3055974/150‑1 (MQ=255)

                                                                                             gTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGa                                                         <  2:5406129/150‑1 (MQ=255)

                                                                                              tGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAg                                                        >  1:1987443/1‑150 (MQ=255)

                                                                                               gCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGt                                                       <  1:3930379/150‑1 (MQ=255)

                                                                                                  gTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGAGCGCCATGCTGGt                                                                                >  2:4749685/1‑122 (MQ=255)

                                                                                                       tggtggTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                               <  1:3830725/102‑1 (MQ=255)

                                                                                                       tggtggTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                               >  2:3830725/1‑102 (MQ=255)

                                                                                                         gtggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGc                                             <  1:2528431/150‑1 (MQ=255)

                                                                                                         gtggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGc                                             <  2:2949912/150‑1 (MQ=255)

                                                                                                           ggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGt                                           <  1:3984822/150‑1 (MQ=255)

                                                                                                                   gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                                   <  1:4430110/150‑1 (MQ=255)

                                                                                                                   gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                                   <  1:5207189/150‑1 (MQ=255)

                                                                                                                       gCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGtgctg                               <  2:2683257/150‑1 (MQ=255)

                                                                                                                        cTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCCTGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGtgctgc                              >  2:3073490/1‑150 (MQ=255)

                                                                                                                          gcgcTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCg                            >  1:2338170/1‑150 (MQ=255)

                                                                                                                            gcTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCt                          <  2:1408670/150‑1 (MQ=255)

                                                                                                                              tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAgg                                                                           <  2:453617/99‑1 (MQ=255)

                                                                                                                              tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                                  >  2:1980008/1‑108 (MQ=255)

                                                                                                                              tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGt                        <  1:1973960/150‑1 (MQ=255)

                                                                                                                              tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGt                        >  2:5119255/1‑150 (MQ=255)

                                                                                                                                 gCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCAc                     <  1:1302210/150‑1 (MQ=255)

                                                                                                                                  ccAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACg                    >  1:3425033/1‑150 (MQ=255)

                                                                                                                                    aGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTc                  >  1:2936894/1‑150 (MQ=255)

                                                                                                                                    aGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTc                  >  2:2195318/1‑150 (MQ=255)

                                                                                                                                      cgcCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTg                >  1:5120129/1‑150 (MQ=255)

                                                                                                                                        gaaGGCCGCCAGCCAGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCa              >  1:3611519/3‑150 (MQ=255)

                                                                                                                                         aaGGCCGCCAGCCAGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCaa             >  1:4826205/2‑150 (MQ=255)

                                                                                                                                          aGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGAGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAAt            >  2:4593277/1‑150 (MQ=255)

                                                                                                                                             ccgccTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGa         >  2:4442804/1‑150 (MQ=255)

                                                                                                                                               gccTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGc       <  2:95224/150‑1 (MQ=255)

ATTGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTTTAC  >  NC_002947/2562281‑2562578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`