BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,562,431	T→A	6.3%	L143Q (CTG→CAG)	PP_2243 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,562,431	0	T	A	6.3%	514.8 / 17.2	159	L143Q (CTG→CAG)	PP_2243	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (68/80); new base A (4/6); total (73/86)
Fisher's exact test for biased strand distribution p-value = 7.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 165 total aligned reads displayed.

TGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTTTACC  >  NC_002947/2562283‑2562579

tGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCctggctg                                                                                                                                                     >  2:830480/1‑150 (MQ=255)

     ccTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACATGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCTGGATCCGGCCAGTGCCGTGGATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCGAGGCCGCGTGGCTggcggc                                                                                                                                                <  1:1165236/150‑1 (MQ=255)

     ccTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAggcggc                                                                                                                                                <  2:1362617/150‑1 (MQ=255)

      cTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTggcgctg                                                                                                                                               <  2:4695389/150‑4 (MQ=255)

      cTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGcc                                                                                                                                               >  2:193301/1‑150 (MQ=255)

       ttGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCGGGCGGCCt                                                                                                                                              >  2:1031210/1‑150 (MQ=255)

        tGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCtt                                                                                                                                             <  2:3983062/150‑1 (MQ=255)

        tGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtg                                                                                                                                             <  1:4964430/150‑2 (MQ=255)

        tGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCAGCCAGGCGGCCtg                                                                                                                                             <  1:4964398/150‑2 (MQ=255)

          ggCGTTGGCAAGGTGTGTCGTTGAAGCAACTACCAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCg                                                                                                                                           <  2:2560185/150‑1 (MQ=255)

          ggCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCg                                                                                                                                           >  2:4323029/1‑150 (MQ=255)

            cGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGCCtggcgc                                                                                                                                         <  2:1695041/150‑6 (MQ=255)

            cGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCAAGGCCGCCAGCCAGGCGGCCtggcgc                                                                                                                                         <  2:1695093/150‑6 (MQ=255)

             ggtGGCAAGGTGTGTCGTTGAAGCAACTACACGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACa                                                                                                                                        <  1:1897967/148‑1 (MQ=255)

             gTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACa                                                                                                                                        <  1:560414/150‑1 (MQ=255)

             gTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACa                                                                                                                                        <  2:2218270/150‑1 (MQ=255)

              ttGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCtgtcgaag                                                                                                                                       <  1:3646932/150‑8 (MQ=255)

                 gCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGcc                                                                                                                                    <  1:3453037/150‑1 (MQ=255)

                   aaGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCgg                                                                                                                                  >  2:4123546/1‑150 (MQ=255)

                   aaGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCgg                                                                                                                                  <  2:3776606/150‑1 (MQ=255)

                       tgtgtCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGa                                                                                                                              <  1:5064375/150‑1 (MQ=255)

                           tCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActgg                                                                                                                          <  1:105087/150‑1 (MQ=255)

                             gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggct                                                                                                                        >  1:95297/1‑150 (MQ=255)

                             gTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggct                                                                                                                        >  1:2809263/1‑150 (MQ=255)

                              ttGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGActggctg                                                                                                                       >  2:3262051/1‑150 (MQ=255)

                                   gCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGt                                                                                                                  >  1:3105407/1‑150 (MQ=255)

                                     aaCTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGa                                                                                                                <  1:4375243/150‑1 (MQ=255)

                                        tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                             <  1:5419021/150‑1 (MQ=255)

                                        tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                             >  1:5197700/1‑150 (MQ=255)

                                        tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                             <  1:4508887/150‑1 (MQ=255)

                                        tACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccc                                                                                                             <  1:2382302/150‑1 (MQ=255)

                                         aCAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGaccca                                                                                                            >  2:491821/1‑150 (MQ=255)

                                           aaGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGacccacc                                                                                                          >  2:3112906/1‑150 (MQ=255)

                                             gCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCg                                                                                                        >  2:3418103/1‑150 (MQ=255)

                                             gCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCg                                                                                                        >  2:2007604/1‑150 (MQ=255)

                                             gCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTAGACAGGCCCGGTGACTGGCTGGCGGTGACCCACCCg                                                                                                        >  1:903430/1‑150 (MQ=255)

                                                gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                     <  1:846700/150‑1 (MQ=255)

                                                gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                     <  1:257128/150‑1 (MQ=255)

                                                gAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATg                                                                                                     <  1:4897881/150‑1 (MQ=255)

                                                 aGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATgg                                                                                                    <  1:2679215/150‑1 (MQ=255)

                                                     aCCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                                <  2:3331942/150‑1 (MQ=255)

                                                              gTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCAt                                                                                       <  1:597652/150‑1 (MQ=255)

                                                               ttGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATg                                                                                      >  2:1200130/1‑150 (MQ=255)

                                                                tGGCCGAATGGCTGCAGGATCCGGCCAGCGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATg                                                                                               <  1:5388317/140‑1 (MQ=255)

                                                                tGGCCGAATGGCTGCAGGATCCGGCCAGCGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATg                                                                                               >  2:5388317/1‑140 (MQ=255)

                                                                 ggCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCt                                                                                    >  2:1850552/1‑150 (MQ=255)

                                                                    cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                                 <  2:5169835/150‑1 (MQ=255)

                                                                    cGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                                 >  2:292739/1‑150 (MQ=255)

                                                                        tGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAg                                                                             <  2:476951/150‑1 (MQ=255)

                                                                            tGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGc                                                                         >  1:1861487/1‑150 (MQ=255)

                                                                              cAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCtt                                                                       <  1:1363047/150‑1 (MQ=255)

                                                                              cAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCtt                                                                       >  1:547480/1‑150 (MQ=255)

                                                                               aGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTg                                                                      >  2:5027773/1‑150 (MQ=255)

                                                                                ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                     <  1:1845282/150‑1 (MQ=255)

                                                                                ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                     <  1:4224131/150‑1 (MQ=255)

                                                                                ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                     <  1:830480/150‑1 (MQ=255)

                                                                                ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                     <  1:3365208/150‑1 (MQ=255)

                                                                                ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                     <  2:1685896/150‑1 (MQ=255)

                                                                                ggATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTgg                                                                     <  2:742809/150‑1 (MQ=255)

                                                                                      ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                               >  1:1433911/1‑150 (MQ=255)

                                                                                      ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                               >  1:1433723/1‑150 (MQ=255)

                                                                                      ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                               >  1:301302/1‑150 (MQ=255)

                                                                                      ggCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTcc                                                               <  1:3622661/150‑1 (MQ=255)

                                                                                         cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTGGACAGGCCGGGTGACTGGGTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGGTTGGGTGTCCgtt                                                            >  1:1428195/1‑148 (MQ=255)

                                                                                         cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGAt                                                            <  2:7722/150‑1 (MQ=255)

                                                                                         cAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGAt                                                            <  2:3055974/150‑1 (MQ=255)

                                                                                           gTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGa                                                          <  2:5406129/150‑1 (MQ=255)

                                                                                            tGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAg                                                         >  1:1987443/1‑150 (MQ=255)

                                                                                             gCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGt                                                        <  1:3930454/150‑1 (MQ=255)

                                                                                             gCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGt                                                        <  1:3930379/150‑1 (MQ=255)

                                                                                                gTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGt                                                                                 <  1:4749685/122‑1 (MQ=255)

                                                                                                gTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGAGCGCCATGCTGGt                                                                                 >  2:4749685/1‑122 (MQ=255)

                                                                                                     tggtggTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                                <  1:3830725/102‑1 (MQ=255)

                                                                                                     tggtggTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCAt                                                                                                >  2:3830725/1‑102 (MQ=255)

                                                                                                       gtggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGc                                              <  2:2949912/150‑1 (MQ=255)

                                                                                                       gtggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGc                                              <  1:2528431/150‑1 (MQ=255)

                                                                                                         ggtgAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGt                                            <  1:3984822/150‑1 (MQ=255)

                                                                                                                 gTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGt                                    <  1:4430237/150‑1 (MQ=255)

                                                                                                                      cTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCCTGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGtgctgc                               >  2:3073490/1‑150 (MQ=255)

                                                                                                                       tGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGcc                              >  1:5228762/1‑150 (MQ=255)

                                                                                                                        gcgcTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCg                             >  1:2338170/1‑150 (MQ=255)

                                                                                                                          gcTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCt                           <  2:1408670/150‑1 (MQ=255)

                                                                                                                            tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAgg                                                                            >  1:453617/1‑99 (MQ=255)

                                                                                                                            tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAgg                                                                            <  2:453617/99‑1 (MQ=255)

                                                                                                                            tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGgt                                                                   >  2:1980008/1‑108 (MQ=255)

                                                                                                                            tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGt                         <  1:1973960/150‑1 (MQ=255)

                                                                                                                            tATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGt                         >  2:5119255/1‑150 (MQ=255)

                                                                                                                               gCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCAc                      <  1:1302210/150‑1 (MQ=255)

                                                                                                                                ccAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACg                     >  1:3425033/1‑150 (MQ=255)

                                                                                                                                  aGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTc                   >  1:4253288/1‑150 (MQ=255)

                                                                                                                                  aGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTc                   >  1:2936894/1‑150 (MQ=255)

                                                                                                                                    cgcCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTg                 >  1:5120129/1‑150 (MQ=255)

                                                                                                                                       aaGGCCGCCAGCCAGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGTAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCaa              >  2:1867831/2‑150 (MQ=255)

                                                                                                                                       aaGGCCGCCAGCCAGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCaa              >  1:4826205/2‑150 (MQ=255)

                                                                                                                                        aGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGAGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAAt             >  2:4593277/1‑150 (MQ=255)

                                                                                                                                        aGGCCGCCAGCCAGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAAt             >  2:2631026/1‑150 (MQ=255)

                                                                                                                                           ccgccTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGa          >  2:4442804/1‑150 (MQ=255)

                                                                                                                                             gccTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGAc                                      >  1:3660442/1‑120 (MQ=255)

                                                                                                                                             gccTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGc        <  2:95224/150‑1 (MQ=255)

                                                                                                                                                   ctggcGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTTTAcc  <  2:2809263/150‑1 (MQ=255)

TGCGACCTTGGGCGTTGGCAAGGTGTGTCGTTGAAGCAACTACAAGCCGAGCAACCGCAGGCGTTGGCCGAATGGCTGCAGGATCCGGCCAGTGCCGTGCATGGTGGTGAGTCGTTCGCTGCGCTATGCCAGCGCCAGGCCGCCTGGCTGGCGGCCTTCGACAGGCCGGGTGACTGGCTGGCGGTGACCCACCCGATGGTCATGCGCGCCATGCTGGTACAGGTGCTTGGGTGTCCGATGAGTGCCGGGCAGCGTATCGACGTGCTGCCGCTGTCACGTCTGCAATTGAGCTTTACC  >  NC_002947/2562283‑2562579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`