BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,667,674	G→A	5.6%	P524P (CCG→CCA)	acnA‑II →	aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,667,674	0	G	A	5.6%	498.1 / 12.3	160	P524P (CCG→CCA)	acnA‑II	aconitate hydratase
Reads supporting (aligned to +/- strand): ref base G (67/83); new base A (4/5); total (71/89)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 160 total aligned reads displayed.

GCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAG  >  NC_002947/2667526‑2667821

gCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGc                                                                                                                                                    >  1:3829680/1‑150 (MQ=255)

    cGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggt                                                                                                                                                <  2:2243070/150‑1 (MQ=255)

       tATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggt                                                                                                                                             >  1:4954391/1‑150 (MQ=255)

       tATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggt                                                                                                                                             >  1:3392133/1‑150 (MQ=255)

        aTGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggtg                                                                                                                                            <  2:2526302/150‑1 (MQ=255)

        aTGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggtg                                                                                                                                            <  2:2647914/150‑1 (MQ=255)

          gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGc                                                                                                                                          <  1:292039/150‑1 (MQ=255)

          gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGc                                                                                                                                          >  2:5442163/1‑150 (MQ=255)

             cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTa                                                                                                                                       >  2:1530019/1‑150 (MQ=255)

             cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTa                                                                                                                                       <  2:3268941/150‑1 (MQ=255)

             cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTa                                                                                                                                       >  2:1530042/1‑150 (MQ=255)

             cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGCgaa                                                                                                                                       <  1:642435/150‑3 (MQ=255)

             cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGCgaa                                                                                                                                       <  2:5443246/150‑3 (MQ=255)

              ggCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTAt                                                                                                                                      >  1:1577880/1‑150 (MQ=255)

              ggCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTAt                                                                                                                                      >  2:950495/1‑150 (MQ=255)

               gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                     <  1:687027/150‑1 (MQ=255)

               gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                     >  1:2378575/1‑150 (MQ=255)

                 gcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGcc                                                                                                                                   <  2:5075620/150‑1 (MQ=255)

                    cTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCAtt                                                                                                                                <  2:2281835/150‑1 (MQ=255)

                       gACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGcc                                                                                                                             >  2:1764293/1‑150 (MQ=255)

                        aCCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCg                                                                                                                            <  1:1973539/150‑1 (MQ=255)

                         cccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGccag                                                                                                                           <  2:5116463/150‑3 (MQ=255)

                         cccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCgg                                                                                                                           <  1:247635/150‑1 (MQ=255)

                          ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                          >  1:1784144/1‑150 (MQ=255)

                          ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                          >  1:1058460/1‑150 (MQ=255)

                           cGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTa                                                                                                                         <  2:1787797/150‑1 (MQ=255)

                                 accAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTc                                                                                                                   <  2:5092805/149‑1 (MQ=255)

                                   cAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGt                                                                                                                 <  1:3622978/150‑1 (MQ=255)

                                     gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               <  2:1566085/150‑1 (MQ=255)

                                     gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               <  2:4274513/150‑1 (MQ=255)

                                     gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               <  2:1565668/150‑1 (MQ=255)

                                     gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               >  1:4345541/1‑150 (MQ=255)

                                       aaGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCCACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTGCGCCACCGCTGGTGGTGGCGTATGCCCTTGCCGGTACCATTCgggtcg                                                                                                             >  2:4710828/1‑145 (MQ=255)

                                        aGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGa                                                                                                            <  1:2863844/150‑1 (MQ=255)

                                            atcatcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATc                                                                                                        >  2:4421067/1‑150 (MQ=255)

                                            atcatcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATc                                                                                                        >  2:5330881/1‑150 (MQ=255)

                                              catcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCga                                                                                                      <  1:1052567/150‑1 (MQ=255)

                                              catcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCga                                                                                                      <  1:1052536/150‑1 (MQ=255)

                                                 cGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGaa                                                                                                   <  1:1492967/150‑1 (MQ=255)

                                                   aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 <  1:1209946/150‑1 (MQ=255)

                                                   aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGGATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 >  2:3127417/1‑150 (MQ=255)

                                                    ccGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGa                                                                                                >  2:3853051/1‑150 (MQ=255)

                                                     cgcgACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGAt                                                                                               <  2:3969437/150‑1 (MQ=255)

                                                           cTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTg                                                                                         >  1:3294397/1‑150 (MQ=255)

                                                            tGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTgg                                                                                        >  2:3992719/1‑150 (MQ=255)

                                                                       gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  2:3956895/150‑1 (MQ=255)

                                                                       gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  1:731506/150‑1 (MQ=255)

                                                                       gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  2:2823262/150‑1 (MQ=255)

                                                                       gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  1:3466086/150‑1 (MQ=255)

                                                                        ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                            <  1:2184773/150‑1 (MQ=255)

                                                                        ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                            <  1:2184825/150‑1 (MQ=255)

                                                                        ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                            <  1:3210725/150‑1 (MQ=255)

                                                                          gtgtTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGc                                                                          >  1:125968/1‑150 (MQ=255)

                                                                                tCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGaa                                                                    >  1:4536696/1‑150 (MQ=255)

                                                                                  gggTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAAt                                                                  >  2:1041232/1‑150 (MQ=255)

                                                                                   ggTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATc                                                                 <  2:3829680/150‑1 (MQ=255)

                                                                                    gTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATcc                                                                <  2:3067879/150‑1 (MQ=255)

                                                                                      aaCCGCAACTTCGACGGGCGTATCCACCCGTACGTCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                              <  2:2268862/150‑1 (MQ=255)

                                                                                      aaCCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                              <  2:1174766/150‑1 (MQ=255)

                                                                                      aaCCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                              <  2:3138122/150‑1 (MQ=255)

                                                                                      aaCCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                              <  1:2083638/150‑1 (MQ=255)

                                                                                         cGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTc                                                           >  1:4066985/1‑150 (MQ=255)

                                                                                         cGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTc                                                           >  1:853176/1‑150 (MQ=255)

                                                                                         cGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTc                                                           >  2:2494585/1‑150 (MQ=255)

                                                                                               ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           <  1:3658038/112‑1 (MQ=255)

                                                                                               ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           >  2:3658038/1‑112 (MQ=255)

                                                                                               ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           <  1:3657995/112‑1 (MQ=255)

                                                                                               ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGAc                                                     >  1:1790387/1‑150 (MQ=255)

                                                                                                 gtaCGGGTGGATACGCCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACAt                                                   >  1:1869385/3‑150 (MQ=255)

                                                                                                    cGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTg                                                <  1:5375755/150‑1 (MQ=255)

                                                                                                    cGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTg                                                <  1:5375767/150‑1 (MQ=255)

                                                                                                     gggCGTCTCCACCCGTACGCCAAGCAGGCCTTCCTGGCGTCGCCACCGCGGGGGGTGGCGTATGCCCTTGCCGGTACCAGTCGGTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCCAGGAAATCCGCCTCAAGGACATCTgg                                               >  2:177288/1‑150 (MQ=255)

                                                                                                     gggCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTgg                                               <  1:2830119/150‑1 (MQ=255)

                                                                                                       gCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGcc                                             >  2:518770/1‑150 (MQ=255)

                                                                                                         gTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCa                                           <  2:1882936/150‑1 (MQ=255)

                                                                                                          tATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAg                                          <  1:4717897/150‑1 (MQ=255)

                                                                                                              cACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgac                                      <  1:157009/150‑1 (MQ=255)

                                                                                                                cccGTACGACAAGCAGGCCTTCCTGGCTTCGCCAACGCTGGTGGTGGCGTATTCCATTGCCGGTACCATTAGTTTCGACATCGAGACGGAAGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgacga                                    >  2:1429164/1‑150 (MQ=255)

                                                                                                                 ccGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACgaa                                   <  1:851300/150‑1 (MQ=255)

                                                                                                                 ccGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACgaa                                   <  1:4338665/150‑1 (MQ=255)

                                                                                                                      cGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGACGaaag                              >  2:3180031/1‑149 (MQ=255)

                                                                                                                       gCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATc                             >  1:2638605/1‑150 (MQ=255)

                                                                                                                       gCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATc                             <  2:5122933/150‑1 (MQ=255)

                                                                                                                        ccAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTc                                                                                >  2:2073691/1‑98 (MQ=255)

                                                                                                                        ccAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCg                            >  2:3003647/1‑150 (MQ=255)

                                                                                                                         cAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGa                           <  1:950495/150‑1 (MQ=255)

                                                                                                                          aaGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGAt                          >  2:1702139/1‑150 (MQ=255)

                                                                                                                          aaGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGAt                          >  1:3961357/1‑150 (MQ=255)

                                                                                                                              aGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGcag                      <  2:687658/150‑3 (MQ=255)

                                                                                                                                        ggCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 <  2:1247692/65‑1 (MQ=255)

                                                                                                                                         gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggtggt                  <  1:5096615/143‑1 (MQ=255)

                                                                                                                                         gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGCCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCa           <  1:1530019/150‑1 (MQ=255)

                                                                                                                                         catacGCCACCACCAGCGGTGGCGAATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcgtgcc           >  2:23001/5‑149 (MQ=255)

                                                                                                                                           ttCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGc         >  1:2758394/1‑150 (MQ=255)

                                                                                                                                           tacGCCACCACCAGCGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAAt                                                                  >  2:4535774/3‑93 (MQ=255)

                                                                                                                                           tacGCCACCACCAGCGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAAt                                                                  <  1:4535774/91‑1 (MQ=255)

                                                                                                                                            tCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg        <  1:3003647/150‑1 (MQ=255)

                                                                                                                                              gCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGt      <  1:1671776/150‑1 (MQ=255)

                                                                                                                                               ccACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTc     >  1:844239/1‑150 (MQ=255)

                                                                                                                                                cACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCa    <  2:4345541/150‑1 (MQ=255)

GCAACGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAG  >  NC_002947/2667526‑2667821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`