BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,667,676	T→C	5.9%	L525P (CTG→CCG) ‡	acnA‑II →	aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,667,676	0	T	C	5.9%	474.8 / 10.8	151	L525P (CTG→CCG) ‡	acnA‑II	aconitate hydratase
Reads supporting (aligned to +/- strand): ref base T (62/79); new base C (4/5); total (67/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 158 total aligned reads displayed.

CGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAG  >  NC_002947/2667530‑2667821

cGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggt                                                                                                                                                <  2:2243070/150‑1 (MQ=255)

cGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggt                                                                                                                                                <  2:2337508/150‑1 (MQ=255)

   tATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggt                                                                                                                                             >  1:3392133/1‑150 (MQ=255)

    aTGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggtg                                                                                                                                            <  2:2526302/150‑1 (MQ=255)

      gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGc                                                                                                                                          >  2:5442163/1‑150 (MQ=255)

      gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCAATGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGc                                                                                                                                          <  2:2103161/150‑1 (MQ=255)

         cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTa                                                                                                                                       >  2:1530019/1‑150 (MQ=255)

         cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGCgaa                                                                                                                                       <  2:5443246/150‑3 (MQ=255)

          ggCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTAt                                                                                                                                      >  2:950495/1‑150 (MQ=255)

          ggCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTAt                                                                                                                                      >  1:1577880/1‑150 (MQ=255)

           gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                     >  1:2378575/1‑150 (MQ=255)

           gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                     >  2:3969698/1‑150 (MQ=255)

             gcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGcc                                                                                                                                   <  2:5075620/150‑1 (MQ=255)

                cTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCAtt                                                                                                                                <  2:2281835/150‑1 (MQ=255)

                   gACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGcc                                                                                                                             >  2:1764293/1‑150 (MQ=255)

                    aCCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCg                                                                                                                            <  2:153963/150‑1 (MQ=255)

                     cccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGccag                                                                                                                           <  2:5116463/150‑3 (MQ=255)

                      ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                          >  1:1784144/1‑150 (MQ=255)

                      ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                          >  1:1058460/1‑150 (MQ=255)

                      ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                          >  2:2016105/1‑150 (MQ=255)

                       cGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTa                                                                                                                         <  2:1787797/150‑1 (MQ=255)

                             accAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTc                                                                                                                   <  2:5092805/149‑1 (MQ=255)

                               cAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGt                                                                                                                 <  1:3622978/150‑1 (MQ=255)

                                aGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGtt                                                                                                                >  1:2605888/1‑150 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               <  2:1566085/150‑1 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               >  1:4345541/1‑150 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                               <  2:4274513/150‑1 (MQ=255)

                                  cAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTc                                                                                                              <  2:1462099/150‑1 (MQ=255)

                                   aaGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCCACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTGCGCCACCGCTGGTGGTGGCGTATGCCCTTGCCGGTACCATTCgggtcg                                                                                                             >  2:4710828/1‑145 (MQ=255)

                                    aGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGa                                                                                                            <  1:2863844/150‑1 (MQ=255)

                                        atcatcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATc                                                                                                        >  2:5330881/1‑150 (MQ=255)

                                        atcatcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATc                                                                                                        >  2:4421067/1‑150 (MQ=255)

                                          catcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCga                                                                                                      <  1:1052567/150‑1 (MQ=255)

                                          catcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCga                                                                                                      <  1:1052536/150‑1 (MQ=255)

                                            tcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCgaga                                                                                                    <  1:3609338/150‑1 (MQ=255)

                                              gACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAg                                                                                                  <  2:2497813/150‑1 (MQ=255)

                                               aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 <  1:1209946/150‑1 (MQ=255)

                                               aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 >  2:4689156/1‑150 (MQ=255)

                                               aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGGATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 >  2:3127417/1‑150 (MQ=255)

                                                ccGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGa                                                                                                >  2:3853051/1‑150 (MQ=255)

                                                    gACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGAtgtg                                                                                            >  1:4413939/1‑150 (MQ=255)

                                                        tGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTgg                                                                                        >  2:3992719/1‑150 (MQ=255)

                                                            cGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGt                                                                                    >  1:2113577/1‑150 (MQ=255)

                                                              ccACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTgg                                                                                  <  1:5373628/150‑1 (MQ=255)

                                                               cACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGt                                                                                 >  2:3156678/1‑150 (MQ=255)

                                                                   gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  2:2823262/150‑1 (MQ=255)

                                                                   gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  1:731506/150‑1 (MQ=255)

                                                                   gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                             <  2:3956895/150‑1 (MQ=255)

                                                                    ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                            <  1:2184773/150‑1 (MQ=255)

                                                                    ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                            <  1:3210725/150‑1 (MQ=255)

                                                                      gtgtTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGc                                                                          >  1:125968/1‑150 (MQ=255)

                                                                        gttgtCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCaa                                                                        >  1:776717/1‑150 (MQ=255)

                                                                        gttgtCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCaa                                                                        <  2:1316367/150‑1 (MQ=255)

                                                                         ttgtCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAg                                                                       <  1:283094/150‑1 (MQ=255)

                                                                            tCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGaa                                                                    >  1:4536696/1‑150 (MQ=255)

                                                                            tCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCAATGGCAAGGaa                                                                    >  1:2932402/1‑150 (MQ=255)

                                                                              gggTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAAt                                                                  >  2:1041232/1‑150 (MQ=255)

                                                                               ggTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATc                                                                 <  2:3829680/150‑1 (MQ=255)

                                                                                gTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATcc                                                                <  2:3067879/150‑1 (MQ=255)

                                                                                     cGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTc                                                           >  1:4066985/1‑150 (MQ=255)

                                                                                      gCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCCTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCa                                                          >  2:2723279/1‑150 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           <  1:3657995/112‑1 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           <  1:3658038/112‑1 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           >  2:3658038/1‑112 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                           >  2:3657995/1‑112 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGAc                                                     >  1:1430603/1‑150 (MQ=255)

                                                                                             gtaCGGGTGGATACGCCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACAt                                                   >  1:1869385/3‑150 (MQ=255)

                                                                                                cGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTg                                                <  1:5375767/150‑1 (MQ=255)

                                                                                                   gCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGcc                                             >  2:518770/1‑150 (MQ=255)

                                                                                                      tATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAg                                          <  2:2270737/150‑1 (MQ=255)

                                                                                                      tATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAg                                          <  1:4717897/150‑1 (MQ=255)

                                                                                                          cACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgac                                      <  1:157009/150‑1 (MQ=255)

                                                                                                           aCCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgacg                                     >  1:18288/1‑150 (MQ=255)

                                                                                                             ccGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACgaa                                   <  1:851344/150‑1 (MQ=255)

                                                                                                             ccGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACgaa                                   <  1:851300/150‑1 (MQ=255)

                                                                                                                  cGCCAAGCAGGCCTTCCTTGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCACGGACATCTGGCCCAGCGACGAAGAAAt                              <  2:1728474/150‑1 (MQ=255)

                                                                                                                  cGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGACGaaag                              >  2:3180031/1‑149 (MQ=255)

                                                                                                                   gCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATc                             <  2:5122933/150‑1 (MQ=255)

                                                                                                                    ccAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTc                                                                                <  1:2073691/98‑1 (MQ=255)

                                                                                                                    ccAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCg                            >  1:2278697/1‑150 (MQ=255)

                                                                                                                     cAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGa                           <  2:1773735/150‑1 (MQ=255)

                                                                                                                     cAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGa                           <  1:950488/150‑1 (MQ=255)

                                                                                                                      aaGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGAt                          >  1:3961357/1‑150 (MQ=255)

                                                                                                                          aGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGcag                      <  2:687658/150‑3 (MQ=255)

                                                                                                                           ggCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggt                     <  1:4737406/150‑1 (MQ=255)

                                                                                                                              cTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggtggt                  <  1:662020/150‑1 (MQ=255)

                                                                                                                                tCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgc                <  1:5442163/150‑1 (MQ=255)

                                                                                                                                  tggcatacGCCACCACCAGCGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcgt              >  1:3333874/8‑150 (MQ=255)

                                                                                                                                  ctgtgttCGCGTCCGGTGGTGGTGGCGTTTGCCTTTGCCGGTACGATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGTTTTCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcag              <  2:3041491/145‑3 (MQ=255)

                                                                                                                                   tGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcgtg             <  1:1764293/150‑1 (MQ=255)

                                                                                                                                    ggCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                 <  2:1247692/65‑1 (MQ=255)

                                                                                                                                     gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggtggt                  <  1:5096615/143‑1 (MQ=255)

                                                                                                                                     gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGCCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCa           <  1:1530019/150‑1 (MQ=255)

                                                                                                                                       ttCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGc         >  1:2758394/1‑150 (MQ=255)

                                                                                                                                        tCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg        <  1:3003647/150‑1 (MQ=255)

                                                                                                                                        tCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg        <  2:1577880/150‑1 (MQ=255)

                                                                                                                                        acGCCACCACCAGCGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg        >  1:2677036/2‑150 (MQ=255)

                                                                                                                                           ccACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTc     >  1:4326222/1‑150 (MQ=255)

                                                                                                                                            cACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCa    <  2:4345541/150‑1 (MQ=255)

                                                                                                                                              ccGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAg  <  2:1102242/150‑1 (MQ=255)

CGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAG  >  NC_002947/2667530‑2667821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`