BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,667,677	G→A	5.9%	L525L (CTG→CTA) ‡	acnA‑II →	aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,667,677	0	G	A	5.9%	472.1 / 11.3	151	L525L (CTG→CTA) ‡	acnA‑II	aconitate hydratase
Reads supporting (aligned to +/- strand): ref base G (63/79); new base A (4/5); total (67/84)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

CGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAGCCGGA  >  NC_002947/2667530‑2667826

cGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggt                                                                                                                                                     <  2:2243070/150‑1 (MQ=255)

cGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggt                                                                                                                                                     <  2:2337508/150‑1 (MQ=255)

 ggTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtg                                                                                                                                                    <  2:3915827/150‑1 (MQ=255)

   tATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggt                                                                                                                                                  >  1:3392133/1‑150 (MQ=255)

    aTGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCtggtggtg                                                                                                                                                 <  2:2647914/150‑1 (MQ=255)

      gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGc                                                                                                                                               >  2:5442163/1‑150 (MQ=255)

      gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGc                                                                                                                                               <  1:292039/150‑1 (MQ=255)

      gTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCAATGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGc                                                                                                                                               <  2:2103161/150‑1 (MQ=255)

         cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTa                                                                                                                                            >  2:1530019/1‑150 (MQ=255)

         cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTa                                                                                                                                            <  2:3268941/150‑1 (MQ=255)

         cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGCgaa                                                                                                                                            <  1:642435/150‑3 (MQ=255)

         cGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCACCAGCGGTGGCgaa                                                                                                                                            <  2:5443246/150‑3 (MQ=255)

          ggCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTAt                                                                                                                                           >  2:950495/1‑150 (MQ=255)

           gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                          >  1:2378575/1‑150 (MQ=255)

           gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                          <  1:687027/150‑1 (MQ=255)

           gcgcCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATg                                                                                                                                          >  2:3969698/1‑150 (MQ=255)

                   gACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGcc                                                                                                                                  >  2:1764293/1‑150 (MQ=255)

                    aCCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCg                                                                                                                                 <  1:1973539/150‑1 (MQ=255)

                    aCCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCg                                                                                                                                 <  2:153963/150‑1 (MQ=255)

                     cccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCgg                                                                                                                                <  1:247635/150‑1 (MQ=255)

                      ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                               >  2:2016105/1‑150 (MQ=255)

                      ccGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGt                                                                                                                               >  1:1058460/1‑150 (MQ=255)

                             accAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTc                                                                                                                        <  2:5092805/149‑1 (MQ=255)

                               cAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGt                                                                                                                      <  1:3622978/150‑1 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                                    <  2:1565668/150‑1 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                                    >  1:4345541/1‑150 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                                    <  2:5308205/150‑1 (MQ=255)

                                 gCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGttt                                                                                                                    <  2:4274513/150‑1 (MQ=255)

                                  cAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTc                                                                                                                   <  2:1462099/150‑1 (MQ=255)

                                    aGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGa                                                                                                                 <  1:2863844/150‑1 (MQ=255)

                                        atcatcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATc                                                                                                             >  2:4421067/1‑150 (MQ=255)

                                          catcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCga                                                                                                           <  1:1052567/150‑1 (MQ=255)

                                          catcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCga                                                                                                           <  1:1052536/150‑1 (MQ=255)

                                            tcGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCgaga                                                                                                         <  1:3609338/150‑1 (MQ=255)

                                             cGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGaa                                                                                                        <  1:1492967/150‑1 (MQ=255)

                                              gACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAg                                                                                                       <  2:2497813/150‑1 (MQ=255)

                                               aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                      <  1:1209946/150‑1 (MQ=255)

                                               aCCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAgg                                                                                                      >  2:4689156/1‑150 (MQ=255)

                                                       cTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTg                                                                                              >  1:3294397/1‑150 (MQ=255)

                                                        tGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTgg                                                                                             >  2:3992719/1‑150 (MQ=255)

                                                          tACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGc                                                                                           >  2:4397904/1‑150 (MQ=255)

                                                            cGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGt                                                                                         >  1:2113577/1‑150 (MQ=255)

                                                               cACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGt                                                                                      >  2:3156678/1‑150 (MQ=255)

                                                                   gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                                  <  2:3956895/150‑1 (MQ=255)

                                                                   gCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGAt                                                                                  <  1:731506/150‑1 (MQ=255)

                                                                    ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                                 <  1:2184825/150‑1 (MQ=255)

                                                                    ccGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATg                                                                                 <  1:3210725/150‑1 (MQ=255)

                                                                      gtgtTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGc                                                                               >  1:125968/1‑150 (MQ=255)

                                                                        gttgtCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCaa                                                                             <  2:1316367/150‑1 (MQ=255)

                                                                            tCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGaa                                                                         >  1:4536696/1‑150 (MQ=255)

                                                                               ggTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATc                                                                      <  2:3829680/150‑1 (MQ=255)

                                                                                  aaCCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                                   <  2:3138122/150‑1 (MQ=255)

                                                                                  aaCCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                                   <  1:2083638/150‑1 (MQ=255)

                                                                                  aaCCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGc                                                                   <  2:1174766/150‑1 (MQ=255)

                                                                                     cGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTc                                                                >  1:4066985/1‑150 (MQ=255)

                                                                                     cGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTc                                                                >  1:853176/1‑150 (MQ=255)

                                                                                      gCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCCTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCa                                                               >  2:2723279/1‑150 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                                >  2:3657995/1‑112 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                                >  2:3658038/1‑112 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                                <  1:3657995/112‑1 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGc                                                                                                <  1:3658038/112‑1 (MQ=255)

                                                                                           ttCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGAc                                                          >  1:1790387/1‑150 (MQ=255)

                                                                                                cGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTg                                                     <  1:5375755/150‑1 (MQ=255)

                                                                                                 gggCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTgg                                                    <  1:2830119/150‑1 (MQ=255)

                                                                                                   gCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGcc                                                  >  2:518770/1‑150 (MQ=255)

                                                                                                     gTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCa                                                <  2:1882936/150‑1 (MQ=255)

                                                                                                      tATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAg                                               <  2:2270737/150‑1 (MQ=255)

                                                                                                      tATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAg                                               <  1:4717897/150‑1 (MQ=255)

                                                                                                          cACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgac                                           <  1:157009/150‑1 (MQ=255)

                                                                                                           aCCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgacg                                          >  2:3073173/1‑150 (MQ=255)

                                                                                                           aCCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGcgacg                                          >  1:18288/1‑150 (MQ=255)

                                                                                                             ccGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACgaa                                        <  1:851344/150‑1 (MQ=255)

                                                                                                                  cGCCAAGCAGGCCTTCCTTGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCACGGACATCTGGCCCAGCGACGAAGAAAt                                   <  2:1728474/150‑1 (MQ=255)

                                                                                                                   gCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATc                                  >  1:2638605/1‑150 (MQ=255)

                                                                                                                   gCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATc                                  <  2:5122933/150‑1 (MQ=255)

                                                                                                                    ccAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCg                                 >  2:3003647/1‑150 (MQ=255)

                                                                                                                     cAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGa                                <  2:1773735/150‑1 (MQ=255)

                                                                                                                     cAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGa                                <  1:950495/150‑1 (MQ=255)

                                                                                                                      aaGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGAt                               >  1:3961357/1‑150 (MQ=255)

                                                                                                                      aaGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGAt                               <  2:4999606/150‑1 (MQ=255)

                                                                                                                          aGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGcag                           <  2:687658/150‑3 (MQ=255)

                                                                                                                           ggCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggt                          <  1:4737406/150‑1 (MQ=255)

                                                                                                                                tCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgc                     <  1:5442163/150‑1 (MQ=255)

                                                                                                                                  ctgtgttCGCGTCCGGTGGTGGTGGCGTTTGCCTTTGCCGGTACGATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGTTTTCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcag                   <  2:3041491/145‑3 (MQ=255)

                                                                                                                                   tGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcgtg                  <  1:1764293/150‑1 (MQ=255)

                                                                                                                                     gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggtggt                       <  1:5096615/143‑1 (MQ=255)

                                                                                                                                     gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCggtggt                       >  2:5096615/1‑143 (MQ=255)

                                                                                                                                     gCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGCCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCa                <  1:1530019/150‑1 (MQ=255)

                                                                                                                                     catacGCCACCACCAGCGGTGGCGAATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGgtgcgtgcc                >  2:23001/5‑149 (MQ=255)

                                                                                                                                       tacGCCACCACCAGCGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAAt                                                                       <  1:4535774/91‑1 (MQ=255)

                                                                                                                                        tCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg             <  1:3003647/150‑1 (MQ=255)

                                                                                                                                        tCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg             <  2:1577880/150‑1 (MQ=255)

                                                                                                                                        acGCCACCACCAGCGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCg             >  1:2677036/2‑150 (MQ=255)

                                                                                                                                          gCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGt           <  1:1671776/150‑1 (MQ=255)

                                                                                                                                           ccACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTc          >  1:844239/1‑150 (MQ=255)

                                                                                                                                           ccACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTc          >  1:4326222/1‑150 (MQ=255)

                                                                                                                                              ccGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAg       <  2:1102242/150‑1 (MQ=255)

                                                                                                                                                   ggtggtggCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAGCCGGa  <  1:518770/150‑1 (MQ=255)

                                                                                                                                                   ggtggtggCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAGCCGGa  <  1:4959653/150‑1 (MQ=255)

                                                                                                                                                   ggtggtggCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAGCCGGa  <  2:4954391/150‑1 (MQ=255)

CGGTATGTCCGGCGCCCTGGACCCGGTGATCCAGCAAGAAATCATCGACCGCGACCTGTACGCCACTGCCGTGTTGTCGGGTAACCGCAACTTCGACGGGCGTATCCACCCGTACGCCAAGCAGGCCTTCCTGGCTTCGCCACCGCTGGTGGTGGCGTATGCCATTGCCGGTACCATTCGTTTCGACATCGAGAAGGATGTGCTGGGCGTGGTCGATGGCAAGGAAATCCGCCTCAAGGACATCTGGCCCAGCGACGAAGAAATCGATGCGGTGGTGCGTGCAGCGGTCAAGCCGGA  >  NC_002947/2667530‑2667826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`