BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,778,803	C→A	7.0%	A395A (GCC→GCA)	sotB →	sugar efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,778,803	0	C	A	7.0%	563.7 / 22.6	172	A395A (GCC→GCA)	sotB	sugar efflux transporter
Reads supporting (aligned to +/- strand): ref base C (70/90); new base A (10/2); total (80/92)
Fisher's exact test for biased strand distribution p-value = 1.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 174 total aligned reads displayed.

ACCGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGC  >  NC_002947/2778654‑2778951

aCCGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGcc                                                                                                                                                      >  1:678641/1‑150 (MQ=255)

 ccGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCg                                                                                                                                                     >  2:3851424/1‑150 (MQ=255)

  cGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGt                                                                                                                                                    <  1:4635617/150‑1 (MQ=255)

    aTGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTg                                                                                                                                                  >  1:5079878/1‑150 (MQ=255)

     tgtgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGc                                                                                                                                                 <  1:420923/150‑1 (MQ=255)

     tgtgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGc                                                                                                                                                 <  2:3449981/150‑1 (MQ=255)

     tgtgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGc                                                                                                                                                 <  2:2514753/150‑1 (MQ=255)

       tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                               >  2:1475652/1‑150 (MQ=255)

       tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                               <  1:123804/150‑1 (MQ=255)

           cATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTAc                                                                                                                                           >  1:3895769/1‑150 (MQ=255)

              ggCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCg                                                                                                                                        <  2:5241038/150‑1 (MQ=255)

                 gcTGTTCTCGGGTATCTACAACATCGGTATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgc                                                                                                                                      >  1:2144554/1‑149 (MQ=255)

                  cTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCATAGGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTTGTACTGGCCTTTGCCTGTACCCGgcgc                                                                                                                                    <  2:1844213/150‑1 (MQ=255)

                  cTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgcgc                                                                                                                                    <  2:5323523/150‑1 (MQ=255)

                        tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                              >  1:4725843/1‑150 (MQ=255)

                        tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                              <  1:3225706/150‑1 (MQ=255)

                          gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                            <  2:835833/150‑1 (MQ=255)

                          gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                            <  1:2902624/150‑1 (MQ=255)

                                 tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                     >  1:2699099/1‑150 (MQ=255)

                                 tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                     >  2:545398/1‑150 (MQ=255)

                                 tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                     >  1:1557052/1‑150 (MQ=255)

                                  acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCgg                                                                                                                                       <  2:1698878/131‑1 (MQ=255)

                                  acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                    <  2:5068024/150‑1 (MQ=255)

                                    aacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGaca                                                                                                                  >  1:85885/1‑150 (MQ=255)

                                    aacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGaca                                                                                                                  >  1:4788508/1‑150 (MQ=255)

                                       aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               <  1:3942467/150‑1 (MQ=255)

                                       aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               >  2:471559/1‑150 (MQ=255)

                                       aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               <  2:457330/150‑1 (MQ=255)

                                       aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               >  1:2777665/1‑150 (MQ=255)

                                           gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                           >  1:4822729/1‑150 (MQ=255)

                                           gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                           >  1:756599/1‑150 (MQ=255)

                                           gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                           >  2:2273626/1‑150 (MQ=255)

                                            cATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGc                                                                                                          <  1:1061753/150‑1 (MQ=255)

                                               tGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGa                                                                                                       <  2:5358470/150‑1 (MQ=255)

                                                ggCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCATCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGGAGTGGTCGGGCTGGTACTGGGCGTTGCGATTACCCGGGGCTTTCGCGAGGAGTTGAGACGTTGAGCCGAg                                                                                                      <  2:4364126/150‑1 (MQ=255)

                                                   ggtggtGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGcc                                                                                                   >  2:1515459/1‑150 (MQ=255)

                                                    gtggtgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCg                                                                                                  <  2:747797/150‑1 (MQ=255)

                                                          cgcTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAgg                                                                                            >  1:1024268/1‑150 (MQ=255)

                                                           gctgctGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGc                                                                                           <  2:1142468/150‑1 (MQ=255)

                                                             tgctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGATGCCGCTCAGGccc                                                                                         >  2:115953/1‑150 (MQ=255)

                                                              gctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                        <  2:2433538/150‑1 (MQ=255)

                                                                tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      <  2:1049844/150‑1 (MQ=255)

                                                                tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      >  2:3339426/1‑150 (MQ=255)

                                                                tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGAGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      >  1:4359375/1‑150 (MQ=255)

                                                                  ggCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGt                                                                                    >  1:207640/1‑150 (MQ=255)

                                                                   gCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                   <  2:371910/150‑1 (MQ=255)

                                                                   gCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                   <  1:2772783/150‑1 (MQ=255)

                                                                    cAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCg                                                                                  <  2:4179594/150‑1 (MQ=255)

                                                                     aGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                    <  1:3732749/115‑1 (MQ=255)

                                                                         tCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCaa                                                                             <  2:160723/150‑1 (MQ=255)

                                                                          cGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                            <  2:2470495/150‑1 (MQ=255)

                                                                          cGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                            <  1:3618574/150‑1 (MQ=255)

                                                                          cGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                            >  2:3521062/1‑150 (MQ=255)

                                                                              agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                        <  1:4858197/150‑1 (MQ=255)

                                                                              agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                        <  1:598426/150‑1 (MQ=255)

                                                                                 agcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGc                                                                     >  2:856740/1‑150 (MQ=255)

                                                                                     aGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAg                                                                 >  2:1304177/1‑150 (MQ=255)

                                                                                     aGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAg                                                                 <  1:459178/150‑1 (MQ=255)

                                                                                       atggatgTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGc                                                               <  1:339580/150‑1 (MQ=255)

                                                                                             gtgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATAc                                                         <  1:172986/150‑1 (MQ=255)

                                                                                              tgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACt                                                        <  2:2111006/150‑1 (MQ=255)

                                                                                                gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                      >  1:1361795/1‑150 (MQ=255)

                                                                                                     tatCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                       <  1:1646525/144‑1 (MQ=255)

                                                                                                         ggCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                             <  1:1683843/150‑1 (MQ=255)

                                                                                                         ggCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                             <  2:2814231/150‑1 (MQ=255)

                                                                                                          gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                        >  1:603976/1‑106 (MQ=255)

                                                                                                          gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                        <  2:603976/106‑1 (MQ=255)

                                                                                                            cTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGa                                          <  1:4484177/150‑1 (MQ=255)

                                                                                                             tggtggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGccc                                                                                         >  2:1648318/1‑102 (MQ=255)

                                                                                                                tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      <  1:1047504/150‑1 (MQ=255)

                                                                                                                tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      <  1:1407847/150‑1 (MQ=255)

                                                                                                                tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      >  1:1846037/1‑150 (MQ=255)

                                                                                                                tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      >  1:4171654/1‑150 (MQ=255)

                                                                                                                 gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATca                                     <  2:3374727/150‑1 (MQ=255)

                                                                                                                   gcggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATcaac                                   <  1:3836463/150‑1 (MQ=255)

                                                                                                                     ggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATcaacaa                                 <  2:4322165/150‑1 (MQ=255)

                                                                                                                          cGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACg                                                                                                                <  1:2778011/66‑1 (MQ=255)

                                                                                                                           gTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAcccc                           >  1:3892091/1‑150 (MQ=255)

                                                                                                                            tGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAccccc                          <  1:1799763/150‑1 (MQ=255)

                                                                                                                              gCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGc                        >  1:3998806/1‑150 (MQ=255)

                                                                                                                                      cGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACtt                <  1:1304177/150‑1 (MQ=255)

                                                                                                                                       gggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTg               <  2:1003872/150‑1 (MQ=255)

                                                                                                                                        ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg              <  1:3081355/150‑1 (MQ=255)

                                                                                                                                        ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg              <  2:800347/150‑1 (MQ=255)

                                                                                                                                        gccgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCg                         <  2:2782964/135‑1 (MQ=255)

                                                                                                                                         ccgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGt             >  1:2845943/4‑150 (MQ=255)

                                                                                                                                          cgggTACTGGCCGTTGCCAGTACCCGGCGATTACGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTAAGGCCAATACTGCAGCCCATGCCGATAACAACAAAACCCCCGCCAACACATGGtt            >  1:4834276/3‑150 (MQ=255)

                                                                                                                                          cgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            >  2:3747246/3‑150 (MQ=255)

                                                                                                                                          cTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            <  1:1475652/150‑1 (MQ=255)

                                                                                                                                             gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa         <  2:4926813/150‑1 (MQ=255)

                                                                                                                                             gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa         <  1:3851424/150‑1 (MQ=255)

                                                                                                                                              tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac        >  1:1968608/1‑150 (MQ=255)

                                                                                                                                              tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac        >  1:3405213/1‑150 (MQ=255)

                                                                                                                                              tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac        <  2:3940162/150‑1 (MQ=255)

                                                                                                                                                cTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac      >  2:2871048/1‑150 (MQ=255)

                                                                                                                                                cTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac      <  2:1557052/150‑1 (MQ=255)

                                                                                                                                                cTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac      <  2:678641/150‑1 (MQ=255)

                                                                                                                                                 tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAcc     >  1:4513663/1‑150 (MQ=255)

                                                                                                                                                 tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGGTGAACAcc     >  1:2371477/1‑150 (MQ=255)

                                                                                                                                                  ggCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCGGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAccc    <  1:3925217/150‑1 (MQ=255)

ACCGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGC  >  NC_002947/2778654‑2778951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`