BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,778,804	G→A	7.0%	V396I (GTT→ATT) ‡	sotB →	sugar efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,778,804	0	G	A	7.0%	520.4 / 19.7	172	V396I (GTT→ATT) ‡	sotB	sugar efflux transporter
Reads supporting (aligned to +/- strand): ref base G (70/89); new base A (10/2); total (80/92)
Fisher's exact test for biased strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 173 total aligned reads displayed.

CCGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGC  >  NC_002947/2778655‑2778951

 cGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGt                                                                                                                                                    <  1:4635617/150‑1 (MQ=255)

    tgtgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGc                                                                                                                                                 <  2:2514753/150‑1 (MQ=255)

      tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                               >  2:1475652/1‑150 (MQ=255)

      tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                               <  1:123804/150‑1 (MQ=255)

      tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGCGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                               <  1:4562603/150‑1 (MQ=255)

          cATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTAc                                                                                                                                           >  1:3895769/1‑150 (MQ=255)

             ggCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCg                                                                                                                                        <  2:5241038/150‑1 (MQ=255)

                gcTGTTCTCGGGTATCTACAACATCGGTATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgc                                                                                                                                      >  1:2144554/1‑149 (MQ=255)

                gcTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgcg                                                                                                                                     >  1:4926813/1‑150 (MQ=255)

                 cTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgcgc                                                                                                                                    <  2:5323523/150‑1 (MQ=255)

                       tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                              >  1:4725843/1‑150 (MQ=255)

                       tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                              <  1:3225706/150‑1 (MQ=255)

                         gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                            <  1:420698/150‑1 (MQ=255)

                         gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                            <  2:835833/150‑1 (MQ=255)

                          ggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCGGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAg                                                                                                                           <  1:649554/150‑1 (MQ=255)

                               cTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGtt                                                                                                                      <  1:3339834/150‑1 (MQ=255)

                                tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                     >  1:1557052/1‑150 (MQ=255)

                                tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                     >  2:545398/1‑150 (MQ=255)

                                tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                     >  1:2699099/1‑150 (MQ=255)

                                 acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCgg                                                                                                                                       <  2:1698878/131‑1 (MQ=255)

                                 acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                    <  2:5068024/150‑1 (MQ=255)

                                      aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               >  1:2777665/1‑150 (MQ=255)

                                      aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               <  1:3942467/150‑1 (MQ=255)

                                      aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               <  2:457330/150‑1 (MQ=255)

                                      aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                               >  2:471559/1‑150 (MQ=255)

                                        cGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTg                                                                                                             <  2:4515782/150‑1 (MQ=255)

                                          gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                           >  1:756599/1‑150 (MQ=255)

                                          gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                           >  2:2273626/1‑150 (MQ=255)

                                           cATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGc                                                                                                          <  1:1061753/150‑1 (MQ=255)

                                            aTTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGcc                                                                                                         >  2:1943744/1‑150 (MQ=255)

                                              tGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGa                                                                                                       <  2:5358470/150‑1 (MQ=255)

                                                  ggtggtGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGcc                                                                                                   >  2:1515459/1‑150 (MQ=255)

                                                   gtggtgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCg                                                                                                  <  2:747797/150‑1 (MQ=255)

                                                         cgcTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAgg                                                                                            >  1:1024268/1‑150 (MQ=255)

                                                          gctgctGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGc                                                                                           <  2:1142468/150‑1 (MQ=255)

                                                            tgctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGATGCCGCTCAGGccc                                                                                         >  2:115985/1‑150 (MQ=255)

                                                             gctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                        <  2:2433538/150‑1 (MQ=255)

                                                               tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      <  1:3745120/150‑1 (MQ=255)

                                                               tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      <  2:1049844/150‑1 (MQ=255)

                                                               tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      >  2:3339426/1‑150 (MQ=255)

                                                               tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGAGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                      >  1:4359375/1‑150 (MQ=255)

                                                                 ggCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGt                                                                                    <  2:1024268/150‑1 (MQ=255)

                                                                  gCAGTATCGTCTGCAGCCAGATGGTTGTGGCGGATATCGGCGTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                   <  1:2772755/150‑1 (MQ=255)

                                                                  gCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                   <  1:389882/150‑1 (MQ=255)

                                                                  gCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                   <  1:4819390/150‑1 (MQ=255)

                                                                  gCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                   <  2:371910/150‑1 (MQ=255)

                                                                   cAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCg                                                                                  <  2:4179594/150‑1 (MQ=255)

                                                                    aGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                    <  1:3732749/115‑1 (MQ=255)

                                                                        tCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCaa                                                                             <  2:160723/150‑1 (MQ=255)

                                                                         cGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                            <  1:3618574/150‑1 (MQ=255)

                                                                         cGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                            >  2:3521062/1‑150 (MQ=255)

                                                                             agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                        <  1:4858197/150‑1 (MQ=255)

                                                                                agcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGc                                                                     >  2:856740/1‑150 (MQ=255)

                                                                                 gcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCt                                                                    >  2:2488037/1‑150 (MQ=255)

                                                                                 gcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCt                                                                    >  1:5357409/1‑150 (MQ=255)

                                                                                            gtgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATAc                                                         <  1:172986/150‑1 (MQ=255)

                                                                                             tgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACt                                                        <  2:2111006/150‑1 (MQ=255)

                                                                                               gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                      >  1:1361795/1‑150 (MQ=255)

                                                                                                 ggATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAg                                                    >  2:3250514/1‑150 (MQ=255)

                                                                                                    tatCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                       <  1:1646525/144‑1 (MQ=255)

                                                                                                        ggCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                             <  1:1683843/150‑1 (MQ=255)

                                                                                                        ggCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                             <  2:2814231/150‑1 (MQ=255)

                                                                                                         gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                        >  1:603976/1‑106 (MQ=255)

                                                                                                         gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                        <  2:603976/106‑1 (MQ=255)

                                                                                                           cTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGa                                          <  1:4484185/150‑1 (MQ=255)

                                                                                                           cTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGa                                          <  1:4484177/150‑1 (MQ=255)

                                                                                                            tggtggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGccc                                                                                         >  2:1648318/1‑102 (MQ=255)

                                                                                                               tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      <  1:1047504/150‑1 (MQ=255)

                                                                                                               tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      <  1:1407847/150‑1 (MQ=255)

                                                                                                               tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      >  1:1846037/1‑150 (MQ=255)

                                                                                                               tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                      >  1:4171654/1‑150 (MQ=255)

                                                                                                                gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATca                                     <  2:3374727/150‑1 (MQ=255)

                                                                                                                gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATca                                     <  2:1565748/150‑1 (MQ=255)

                                                                                                                    ggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATcaacaa                                 <  2:4322165/150‑1 (MQ=255)

                                                                                                                         cGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACg                                                                                                                <  1:2778011/66‑1 (MQ=255)

                                                                                                                          gTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAcccc                           >  1:3892091/1‑150 (MQ=255)

                                                                                                                           tGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAccccc                          <  1:1799763/150‑1 (MQ=255)

                                                                                                                             gCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGc                        >  1:3998806/1‑150 (MQ=255)

                                                                                                                                     cGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACtt                <  1:1304177/150‑1 (MQ=255)

                                                                                                                                       ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg              <  1:3081355/150‑1 (MQ=255)

                                                                                                                                       ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg              <  1:876921/150‑1 (MQ=255)

                                                                                                                                       ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg              <  2:800347/150‑1 (MQ=255)

                                                                                                                                       gccgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCg                         <  2:2782964/135‑1 (MQ=255)

                                                                                                                                        ccgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGt             >  1:2846041/4‑150 (MQ=255)

                                                                                                                                        ccgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGt             >  1:2845943/4‑150 (MQ=255)

                                                                                                                                         cgggTACTGGCCGTTGCCAGTACCCGGCGATTACGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTAAGGCCAATACTGCAGCCCATGCCGATAACAACAAAACCCCCGCCAACACATGGtt            >  1:4834276/3‑150 (MQ=255)

                                                                                                                                         cgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            >  1:2070212/3‑150 (MQ=255)

                                                                                                                                         cgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            >  1:4771608/3‑150 (MQ=255)

                                                                                                                                         cgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            >  2:3747246/3‑150 (MQ=255)

                                                                                                                                         cgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            >  2:3489217/3‑150 (MQ=255)

                                                                                                                                         cTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt            <  1:1475652/150‑1 (MQ=255)

                                                                                                                                          gggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTg           >  2:3921931/2‑150 (MQ=255)

                                                                                                                                            gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa         <  2:4926813/150‑1 (MQ=255)

                                                                                                                                            gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa         <  1:3851424/150‑1 (MQ=255)

                                                                                                                                             tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac        >  1:1968608/1‑150 (MQ=255)

                                                                                                                                             tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac        <  2:3940162/150‑1 (MQ=255)

                                                                                                                                             tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac        >  1:3405213/1‑150 (MQ=255)

                                                                                                                                               cTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac      <  2:678641/150‑1 (MQ=255)

                                                                                                                                                tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGGTGAACAcc     >  1:2371477/1‑150 (MQ=255)

                                                                                                                                                 ggCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAccc    <  2:1361012/150‑1 (MQ=255)

CCGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGC  >  NC_002947/2778655‑2778951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`