BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,778,805	T→C	7.0%	V396A (GTT→GCT) ‡	sotB →	sugar efflux transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,778,805	0	T	C	7.0%	525.6 / 18.4	171	V396A (GTT→GCT) ‡	sotB	sugar efflux transporter
Reads supporting (aligned to +/- strand): ref base T (70/88); new base C (10/2); total (80/91)
Fisher's exact test for biased strand distribution p-value = 1.37e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 174 total aligned reads displayed.

CGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAAC  >  NC_002947/2778656‑2778954

cGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGt                                                                                                                                                       <  1:4635617/150‑1 (MQ=255)

  aTGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTg                                                                                                                                                     >  1:5079878/1‑150 (MQ=255)

   tgtgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGc                                                                                                                                                    <  1:420923/150‑1 (MQ=255)

     tgGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCa                                                                                                                                                  <  1:123804/150‑1 (MQ=255)

         cATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTAc                                                                                                                                              >  1:3895769/1‑150 (MQ=255)

            ggCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCg                                                                                                                                           <  2:5241038/150‑1 (MQ=255)

               gcTGTTCTCGGGTATCTACAACATCGGTATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgc                                                                                                                                         <  2:2144554/149‑1 (MQ=255)

               gcTGTTCTCGGGTATCTACAACATCGGTATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgc                                                                                                                                         >  1:2144554/1‑149 (MQ=255)

                cTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGgcgc                                                                                                                                       <  2:5323523/150‑1 (MQ=255)

                      tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                                 >  2:148366/1‑150 (MQ=255)

                      tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                                 >  1:4725843/1‑150 (MQ=255)

                      tcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                                 <  1:3225706/150‑1 (MQ=255)

                      gcGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTcgc                                                                                                                                 <  2:2797723/149‑1 (MQ=255)

                        gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                               <  2:835833/150‑1 (MQ=255)

                        gggTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGa                                                                                                                               <  1:2902624/150‑1 (MQ=255)

                               tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                        >  2:545398/1‑150 (MQ=255)

                               tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                        >  1:1557052/1‑150 (MQ=255)

                               tACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTg                                                                                                                        >  1:2699099/1‑150 (MQ=255)

                                acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCgg                                                                                                                                          <  2:1698878/131‑1 (MQ=255)

                                acaacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                       <  2:5068024/150‑1 (MQ=255)

                                  aacaTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGaca                                                                                                                     >  1:4788508/1‑150 (MQ=255)

                                     aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                                  <  1:3942467/150‑1 (MQ=255)

                                     aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                                  >  1:2777665/1‑150 (MQ=255)

                                     aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                                  <  2:457330/150‑1 (MQ=255)

                                     aTCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGt                                                                                                                  >  2:471559/1‑150 (MQ=255)

                                      tCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGtt                                                                                                                 <  1:707189/150‑1 (MQ=255)

                                         gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                              >  1:4822729/1‑150 (MQ=255)

                                         gCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAg                                                                                                              >  1:756599/1‑150 (MQ=255)

                                          cATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGc                                                                                                             <  1:1061753/150‑1 (MQ=255)

                                           aTTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGcc                                                                                                            >  2:1943744/1‑150 (MQ=255)

                                            ttGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCg                                                                                                           >  2:2315227/1‑150 (MQ=255)

                                             tGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGa                                                                                                          <  2:5358470/150‑1 (MQ=255)

                                              ggCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCATCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGGAGTGGTCGGGCTGGTACTGGGCGTTGCGATTACCCGGGGCTTTCGCGAGGAGTTGAGACGTTGAGCCGAg                                                                                                         <  2:4364126/150‑1 (MQ=255)

                                                 ggtggtGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGcc                                                                                                      >  2:1515459/1‑150 (MQ=255)

                                                  gtggtgCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCg                                                                                                     <  2:747797/150‑1 (MQ=255)

                                                        cgcTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAgg                                                                                               >  1:1024268/1‑150 (MQ=255)

                                                         gctgctGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGc                                                                                              <  2:1142468/150‑1 (MQ=255)

                                                           tgctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGATGCCGCTCAGGccc                                                                                            >  2:115985/1‑150 (MQ=255)

                                                           tgctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGccc                                                                                            >  2:3702392/1‑150 (MQ=255)

                                                           tgctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCAACGGCCAGTAcca                                                                                                                                            >  2:1050563/1‑101 (MQ=255)

                                                            gctgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                           <  2:2433538/150‑1 (MQ=255)

                                                              tgGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGAGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCtt                                                                                         >  1:4359375/1‑150 (MQ=255)

                                                                ggCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGt                                                                                       <  2:1024268/150‑1 (MQ=255)

                                                                ggCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGt                                                                                       >  1:207640/1‑150 (MQ=255)

                                                                 gCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTc                                                                                      <  1:2772783/150‑1 (MQ=255)

                                                                  cAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCg                                                                                     <  2:4179594/150‑1 (MQ=255)

                                                                   aGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                       <  1:3732749/115‑1 (MQ=255)

                                                                   aGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGa                                                                                                                       >  2:3732749/1‑115 (MQ=255)

                                                                        cGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAg                                                                               <  1:3618574/150‑1 (MQ=255)

                                                                            agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                           <  1:4858197/150‑1 (MQ=255)

                                                                            agcagcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTaga                                                                           <  1:598426/150‑1 (MQ=255)

                                                                               agcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGc                                                                        >  2:856740/1‑150 (MQ=255)

                                                                                gcCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCt                                                                       >  2:2488037/1‑150 (MQ=255)

                                                                                   aGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAg                                                                    <  1:459178/150‑1 (MQ=255)

                                                                                     atggatgTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGc                                                                  <  1:339580/150‑1 (MQ=255)

                                                                                           gtgGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATAc                                                            <  1:172986/150‑1 (MQ=255)

                                                                                              gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                         >  1:1361795/1‑150 (MQ=255)

                                                                                              gCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGc                                                         >  2:1683843/1‑150 (MQ=255)

                                                                                                ggATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAg                                                       >  2:3250514/1‑150 (MQ=255)

                                                                                                   tatCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                          <  1:1646525/144‑1 (MQ=255)

                                                                                                   tatCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTg                                                          >  2:1646525/1‑144 (MQ=255)

                                                                                                       ggCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                                <  1:1683843/150‑1 (MQ=255)

                                                                                                       ggCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGc                                                <  2:2814231/150‑1 (MQ=255)

                                                                                                        gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                           <  2:603976/106‑1 (MQ=255)

                                                                                                        gCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGcccc                                                                                           >  1:603976/1‑106 (MQ=255)

                                                                                                          cTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGa                                             <  1:4484177/150‑1 (MQ=255)

                                                                                                              tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                         >  1:1846037/1‑150 (MQ=255)

                                                                                                              tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                         <  1:1047504/150‑1 (MQ=255)

                                                                                                              tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                         <  1:1407847/150‑1 (MQ=255)

                                                                                                              tggGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATc                                         >  1:4171654/1‑150 (MQ=255)

                                                                                                               gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATca                                        <  2:3374727/150‑1 (MQ=255)

                                                                                                               gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATca                                        <  2:3374715/150‑1 (MQ=255)

                                                                                                               gggCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATca                                        <  2:1565748/150‑1 (MQ=255)

                                                                                                                 gcggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATcaac                                      <  1:3836463/150‑1 (MQ=255)

                                                                                                                   ggcAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATcaacaa                                    <  2:4322165/150‑1 (MQ=255)

                                                                                                                        cGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACg                                                                                                                   >  2:2778011/1‑66 (MQ=255)

                                                                                                                        cGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACg                                                                                                                   <  1:2778011/66‑1 (MQ=255)

                                                                                                                         gTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAcccc                              >  1:3892091/1‑150 (MQ=255)

                                                                                                                          tGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAAccccc                             <  1:1799763/150‑1 (MQ=255)

                                                                                                                            gCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGc                           >  1:3998806/1‑150 (MQ=255)

                                                                                                                                    cGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACtt                   <  1:1304177/150‑1 (MQ=255)

                                                                                                                                      ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg                 <  1:3081355/150‑1 (MQ=255)

                                                                                                                                      ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg                 <  2:800347/150‑1 (MQ=255)

                                                                                                                                      ggCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTgg                 <  1:876921/150‑1 (MQ=255)

                                                                                                                                       ccgggTACTGGCAACGGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGt                >  1:2845943/4‑150 (MQ=255)

                                                                                                                                        cgggTACTGGCCGTTGCCAGTACCCGGCGATTACGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTAAGGCCAATACTGCAGCCCATGCCGATAACAACAAAACCCCCGCCAACACATGGtt               >  1:4834276/3‑150 (MQ=255)

                                                                                                                                        cgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt               >  2:3747246/3‑150 (MQ=255)

                                                                                                                                        cgggTACTGGCAACGGCCAGTACCAGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt               >  2:3489217/3‑150 (MQ=255)

                                                                                                                                        cTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGtt               <  1:1475652/150‑1 (MQ=255)

                                                                                                                                           gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa            <  2:4926813/150‑1 (MQ=255)

                                                                                                                                           gTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGaa            <  1:3851424/150‑1 (MQ=255)

                                                                                                                                            tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac           >  1:3405213/1‑150 (MQ=255)

                                                                                                                                            tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac           >  1:1968608/1‑150 (MQ=255)

                                                                                                                                            tACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAac           <  2:3940162/150‑1 (MQ=255)

                                                                                                                                              cTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAacac         <  2:678641/150‑1 (MQ=255)

                                                                                                                                               tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAcc        >  1:4513663/1‑150 (MQ=255)

                                                                                                                                               tGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGGTGAACAcc        >  1:2371477/1‑150 (MQ=255)

                                                                                                                                                ggCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCGGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAccc       <  1:3925217/150‑1 (MQ=255)

                                                                                                                                                ggCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACAccc       <  2:1361012/150‑1 (MQ=255)

                                                                                                                                                     ttGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAAc  <  1:672979/150‑1 (MQ=255)

CGATGTGGCCATGGCGCTGTTCTCGGGTATCTACAACATCGGCATTGGCGGTGGTGCGCTGCTGGGCAGTATCGTCAGCAGCCAGATGGATGTGGCGGATATCGGCCTGGTGGGCGGCAGCGTGGCAGTGGTCGGGCTGGTACTGGCCGTTGCCAGTACCCGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAAC  >  NC_002947/2778656‑2778954

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`