BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,778,965	C→G	7.3%	A169P (GCA→CCA)	PP_2429 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,778,965	0	C	G	7.3%	586.3 / 27.3	179	A169P (GCA→CCA)	PP_2429	membrane protein
Reads supporting (aligned to +/- strand): ref base C (94/72); new base G (8/5); total (102/77)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 184 total aligned reads displayed.

CGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACC  >  NC_002947/2778816‑2779108

cGGCGCTTTCGCGAGGCGTGGACCCGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACACAACCCCCGCCAACACTTGGTTGAACACCCGCCACCGCCCCGGTGc                                                                                                                                                  <  2:3701122/150‑1 (MQ=255)

 ggCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGcc                                                                                                                                                 >  2:1655249/1‑150 (MQ=255)

  gcgcTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGTGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCt                                                                                                                                                <  2:4771608/150‑1 (MQ=255)

  gcgcTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCt                                                                                                                                                <  2:4788508/150‑1 (MQ=255)

     cTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCa                                                                                                                                             <  2:756599/150‑1 (MQ=255)

      tttCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAg                                                                                                                                            >  2:2384442/1‑150 (MQ=255)

            gAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGc                                                                                                                                                  <  2:2255623/138‑1 (MQ=255)

            gAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGc                                                                                                                                                  >  1:2255623/1‑138 (MQ=255)

             aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                    <  1:3521062/150‑1 (MQ=255)

             aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                    <  2:4813393/150‑1 (MQ=255)

             aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                    <  1:1729725/150‑1 (MQ=255)

             aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                    >  1:3163257/1‑150 (MQ=255)

             aGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑gg                                                                                                                                    <  2:2777863/150‑1 (MQ=255)

               gCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCC‑GGgc                                                                                                                                  >  1:4293379/1‑150 (MQ=255)

               gCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGgc                                                                                                                                  <  1:4667806/150‑1 (MQ=255)

                 gTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGgcgc                                                                                                                                >  2:2732899/1‑150 (MQ=255)

                     acacGTTGAGCCGATGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTccc                                                                                                                            <  1:115953/150‑1 (MQ=255)

                     acacGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTccc                                                                                                                            >  2:4293455/1‑150 (MQ=255)

                     acacGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTccc                                                                                                                            <  2:2344707/150‑1 (MQ=255)

                       acGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCa                                                                                                                          <  1:2488037/150‑1 (MQ=255)

                       acGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCa                                                                                                                          >  2:416174/1‑150 (MQ=255)

                         gTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCC‑GGGCGCGCcccccc                                                                                                                        >  1:1375411/1‑150 (MQ=255)

                         cctGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCAcc                                                                                                                        >  1:4312913/3‑150 (MQ=255)

                          ttGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCGCCCCAccc                                                                                                                       >  1:1237613/1‑150 (MQ=255)

                          gtGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTcccccccc                                                                                                                       <  2:4424651/149‑1 (MQ=255)

                             aGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCa                                                                                                                    >  2:973240/1‑150 (MQ=255)

                              gCCGAGGCCGCTCAGGGCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCaa                                                                                                                   >  2:4937146/1‑150 (MQ=255)

                              gCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCG‑CCCGGTGGCTGCAGGCACC‑ggggc                                                                                                                                 <  2:207361/135‑3 (MQ=255)

                              gCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCG‑CCCGGTGGCTGCAGGCACC‑ggggc                                                                                                                                 >  1:207361/1‑133 (MQ=255)

                               ccGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAac                                                                                                                  <  2:2255643/150‑1 (MQ=255)

                                 gAGGCCTCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAacac                                                                                                                >  1:640122/1‑150 (MQ=255)

                                 gAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCCCC‑GGGCGCTCCCCACCCCCAacac                                                                                                                >  1:2564078/1‑150 (MQ=255)

                                 gAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAacac                                                                                                                >  1:304505/1‑150 (MQ=255)

                                  aGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACAcc                                                                                                               <  1:5421228/150‑1 (MQ=255)

                                  aGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACAcc                                                                                                               <  1:148366/150‑1 (MQ=255)

                                    gCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACCCCGc                                                                                                             <  2:3895769/150‑1 (MQ=255)

                                              ccccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGcc                                                                                                   >  2:3108439/1‑150 (MQ=255)

                                               cccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCa                                                                                                  >  1:1556800/1‑150 (MQ=255)

                                                ccTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCAt                                                                                                 >  1:688331/1‑150 (MQ=255)

                                                 cTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATg                                                                                                >  2:808385/1‑150 (MQ=255)

                                                  ttGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGc                                                                                               >  2:1488794/1‑150 (MQ=255)

                                                    gTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTc                                                                                             <  1:2384442/150‑1 (MQ=255)

                                                    gTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTc                                                                                             <  2:4359375/150‑1 (MQ=255)

                                                       ggTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGc                                                                                          <  1:856740/150‑1 (MQ=255)

                                                         tCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCag                                                                                        >  1:5158846/1‑150 (MQ=255)

                                                          cAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCACCACCGCCCAGGCGGCCATGCTCGGCaga                                                                                       <  2:2845943/150‑1 (MQ=255)

                                                              tAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGa                                                                                   >  2:4983524/1‑150 (MQ=255)

                                                                gagGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCTGCCGAGcatgg                                                                                 <  2:1810838/150‑5 (MQ=255)

                                                                gagGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCCCCCCGCCGGCCATGCGCGGCAGAGCGATc                                                                                 >  2:575263/1‑150 (MQ=255)

                                                                 agGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCa                                                                                >  2:917124/1‑150 (MQ=255)

                                                                   gCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCaga                                                                                       <  2:5425980/141‑1 (MQ=255)

                                                                     tCAGGCCAATACTGCAGCCCATGGCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCGCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCaa                                                                            <  2:4834276/150‑1 (MQ=255)

                                                                     tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑ggg                                                                                                                                   >  2:4255732/1‑95 (MQ=255)

                                                                     tCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCaa                                                                            <  1:3339426/150‑1 (MQ=255)

                                                                         gCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAac                                                                        <  2:1361795/150‑1 (MQ=255)

                                                                           cAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAacac                                                                      <  1:4321670/150‑1 (MQ=255)

                                                                             aTACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCa                                                                    <  1:545398/150‑1 (MQ=255)

                                                                                   cAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCa                                                              >  2:1686644/1‑150 (MQ=255)

                                                                                   cAGCCCATGCCGATAGCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCa                                                              >  2:255856/1‑150 (MQ=255)

                                                                                      cccATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCt                                                           >  2:2011800/1‑150 (MQ=255)

                                                                                         aTGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGcc                                                        <  2:2564078/150‑1 (MQ=255)

                                                                                                tatCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCCCCCCCAACACCGCCCCGGCGGCCATGCTCGGCAGAGCGCTCAGCAAGAACACCAGCGCCCGCTGCCACACTgg                                                 >  1:3452438/1‑150 (MQ=255)

                                                                                                  tCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCa                                               >  1:2866977/1‑150 (MQ=255)

                                                                                                  tCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCCCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCACGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCa                                               >  1:619736/1‑150 (MQ=255)

                                                                                                    aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGGCTGCAGGCACC‑ggggc                                                                                                                                 >  2:2151678/1‑64 (MQ=38)

                                                                                                    aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAac                                             >  1:4574661/1‑150 (MQ=255)

                                                                                                    aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCACGCGGCCAAGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCAGCCACACAGGCAac                                             >  1:1833506/1‑150 (MQ=255)

                                                                                                    aacaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCG‑CCCGGTGGCTGCAGGCACC‑ggggcg                                                                                                                                <  2:3319397/66‑4 (MQ=25)

                                                                                                     acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCa                                                                                                  >  1:1968683/1‑96 (MQ=255)

                                                                                                     acaaAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCa                                                                                                  <  2:1968683/96‑1 (MQ=255)

                                                                                                       aaaaCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTAGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacgac                                          >  1:3261672/1‑150 (MQ=255)

                                                                                                        acaCCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAacgacg                                         <  2:5357409/148‑1 (MQ=255)

                                                                                                            ccccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGgcgc                                     >  2:67312/1‑150 (MQ=255)

                                                                                                             cccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGcgcg                                    >  2:2612371/1‑150 (MQ=255)

                                                                                                              ccGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCgg                                   >  2:3646952/1‑150 (MQ=255)

                                                                                                                gCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCa                                 >  2:688119/1‑150 (MQ=255)

                                                                                                                 ccAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCaa                                <  2:3998806/150‑1 (MQ=255)

                                                                                                                   aaCACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAAt                              >  2:246336/1‑150 (MQ=255)

                                                                                                                   aaCACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACAGGCAACGACGGCGCGGCAAAt                              >  1:4856372/1‑150 (MQ=255)

                                                                                                                      acTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcc                           <  1:416174/150‑1 (MQ=255)

                                                                                                                       cTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAccc                          <  1:3250514/150‑1 (MQ=255)

                                                                                                                        ttGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATAcccc                         <  2:2371477/150‑1 (MQ=255)

                                                                                                                              gAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGaccac                   >  2:3584031/1‑150 (MQ=255)

                                                                                                                               aaCACCCGCAACCGCCCCGGTGCCCGCAGCCACC‑GGGCGCCCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGaccacc                  <  2:3405213/150‑1 (MQ=255)

                                                                                                                                  acCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCCGGCGGCCATGCTCGGCCGAGCGATCAGCCAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCCCCGcc               >  2:4613866/1‑150 (MQ=255)

                                                                                                                                  acCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCACGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGcc               >  2:3192526/1‑150 (MQ=255)

                                                                                                                                  acCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGcc               >  2:862512/1‑150 (MQ=255)

                                                                                                                                   cccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCa              <  1:4378778/150‑1 (MQ=255)

                                                                                                                                    ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc             >  1:2285949/1‑150 (MQ=255)

                                                                                                                                    ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc             >  1:305571/1‑150 (MQ=255)

                                                                                                                                    ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc             >  2:539655/1‑150 (MQ=255)

                                                                                                                                    ccGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCAc             >  1:2322892/1‑150 (MQ=255)

                                                                                                                                     cGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACg            <  1:575232/150‑1 (MQ=255)

                                                                                                                                        aaCCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGcc         >  1:3742017/1‑150 (MQ=255)

                                                                                                                                          ccGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGAACAGCCAGTACACCAGCGCCAGCAGCCACACTGGCAACGGCGGCGCGGCACATACCCCGACACCCGCCCCGGCCAt       >  1:3805963/1‑150 (MQ=255)

                                                                                                                                           cGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCCCCGCCACGGCCATc      >  1:3675302/1‑150 (MQ=255)

                                                                                                                                           cGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATc      >  1:3675670/1‑150 (MQ=255)

                                                                                                                                             gcccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCa     >  2:151774/2‑150 (MQ=255)

                                                                                                                                               ccGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAc   >  1:1027151/1‑150 (MQ=255)

                                                                                                                                                cGGTGGCTGCAGGCACCGGGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAAcc  >  1:4749413/1‑150 (MQ=255)

CGGCGCTTTCGCGAGGCGTTGACACGTTGAGCCGAGGCCGCTCAGGCCCCTTGTCGGTCAAGTAGAGGCTCAGGCCAATACTGCAGCCCATGCCGATATCAACAAAACCCCCGCCAACACTTGGTTGAACACCCGCAACCGCCCCGGTGCCTGCAGCCACC‑GGGCGCTCCCCACCCCCAACACCGCCCAGGCGGCCATGCTCGGCAGAGCGATCAGCAAGAACACCAGCGCCAGCTGCCACACTGGCAACGACGGCGCGGCAAATACCCCGACCACCGCCACGGCCATCAACC  >  NC_002947/2778816‑2779108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`