BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,893,771	A→T	5.4%	L12Q (CTG→CAG)	PP_2546 ←	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,893,771	0	A	T	5.4%	536.6 / 12.0	158	L12Q (CTG→CAG)	PP_2546	membrane protein
Reads supporting (aligned to +/- strand): ref base A (69/80); new base T (6/3); total (75/83)
Fisher's exact test for biased strand distribution p-value = 3.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TCCCAGGTGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACGCT  >  NC_002947/2893624‑2893918

tCCCAGGTGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTgcagcagc                                                                                                                                                   <  1:786962/150‑1 (MQ=255)

      gtggtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                             >  1:708671/1‑150 (MQ=255)

      gtggtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAgg                                                                                                                                             >  1:708779/1‑150 (MQ=255)

        ggtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgt                                                                                                                                           >  2:2560717/1‑150 (MQ=255)

         gtgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgtg                                                                                                                                          >  1:4138894/1‑150 (MQ=255)

          tgAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGgtgt                                                                                                                                         >  2:3108809/1‑150 (MQ=255)

                ggCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcggcgg                                                                                                                                   >  2:2318311/1‑150 (MQ=255)

                ggCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTcggcgg                                                                                                                                   >  2:2318426/1‑150 (MQ=255)

                 gCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCggcggc                                                                                                                                  >  1:128522/1‑150 (MQ=255)

                   aGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCaa                                                                                                                                >  2:1817886/1‑150 (MQ=255)

                   aGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCaa                                                                                                                                <  2:436643/150‑1 (MQ=255)

                      ccAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGc                                                                                                                             >  1:2991265/1‑150 (MQ=255)

                          gTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTa                                                                                                                         >  1:1668241/1‑150 (MQ=255)

                                gCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAt                                                                                                                   <  1:1493339/150‑1 (MQ=255)

                                  aCTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgg                                                                                                                 >  2:3526616/1‑150 (MQ=255)

                                    tcctccGCCAGGCCCCAGGCCAGGACCGTCACGTTCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCGCCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggt                                                                                                               <  1:4800222/150‑1 (MQ=255)

                                    tCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggt                                                                                                               >  1:3986547/1‑150 (MQ=255)

                                    tCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggt                                                                                                               >  2:152206/1‑150 (MQ=255)

                                    tCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggt                                                                                                               >  2:3977620/1‑150 (MQ=255)

                                    tCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggt                                                                                                               >  1:5336595/1‑150 (MQ=255)

                                     ccACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggtt                                                                                                              >  2:4644598/1‑150 (MQ=255)

                                     ccACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggtt                                                                                                              <  2:1449543/150‑1 (MQ=255)

                                     ccACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggtt                                                                                                              <  1:5310217/150‑1 (MQ=255)

                                       aCCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggttgg                                                                                                            >  1:3089540/1‑150 (MQ=255)

                                       aCCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggttgg                                                                                                            >  1:3140533/1‑150 (MQ=255)

                                       aCCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgg                                                                                                                 >  2:5336245/1‑145 (MQ=255)

                                         cGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCAtgggttgggt                                                                                                          <  2:4318425/150‑1 (MQ=255)

                                          gCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTg                                                                                                         >  1:1331054/1‑150 (MQ=255)

                                          cccaatccccaggccAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTg                                                                                                         >  2:245449/7‑150 (MQ=255)

                                            caggccccaggccAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGcc                                                                                                       >  2:1470537/1‑150 (MQ=255)

                                               gccccaggccAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCgg                                                                                                    >  1:2706772/1‑150 (MQ=255)

                                                ccccaggccAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCggg                                                                                                   >  1:4377938/1‑150 (MQ=255)

                                                 cccaggccAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCgggg                                                                                                  >  1:3766355/1‑150 (MQ=255)

                                                 cccaggccAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCgggg                                                                                                  >  2:3702955/1‑150 (MQ=255)

                                                  ccaggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGa                                                                                                 <  2:4662854/150‑1 (MQ=255)

                                                    aggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGAtt                                                                                               <  2:538072/150‑1 (MQ=255)

                                                     ggccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTg                                                                                              <  2:3986547/150‑1 (MQ=255)

                                                       ccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  1:3963528/150‑1 (MQ=255)

                                                       ccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  1:245449/150‑1 (MQ=255)

                                                       ccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  1:245725/150‑1 (MQ=255)

                                                       ccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  1:3880673/150‑1 (MQ=255)

                                                       ccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  2:3766355/150‑1 (MQ=255)

                                                       ccaggACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTggg                                                                                            <  2:3975999/150‑1 (MQ=255)

                                                                gCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCa                                                                                   <  1:839120/150‑1 (MQ=255)

                                                                gCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCa                                                                                   >  1:2759061/1‑150 (MQ=255)

                                                                 cAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAg                                                                                  >  2:305620/1‑150 (MQ=255)

                                                                   aGTGCAGCACCAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGCTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCa                                                                                <  1:4326653/150‑1 (MQ=255)

                                                                   aGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCa                                                                                <  1:3789157/150‑1 (MQ=255)

                                                                     tGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATa                                                                              <  1:4020976/150‑1 (MQ=255)

                                                                     tGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATa                                                                              >  1:4920694/1‑150 (MQ=255)

                                                                       cagcaACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGt                                                                            <  2:3348436/150‑1 (MQ=255)

                                                                        agcaACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGtt                                                                           >  1:534340/1‑150 (MQ=255)

                                                                          caACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATAGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTgg                                                                         <  2:3589195/150‑1 (MQ=255)

                                                                             cagggcagggCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGtt                                                                      >  1:1563632/1‑150 (MQ=255)

                                                                                   agggcGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGa                                                                >  2:4001077/1‑150 (MQ=255)

                                                                                     ggcGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATc                                                              >  2:4950728/1‑150 (MQ=255)

                                                                                      gcgcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCt                                                             <  2:128522/150‑1 (MQ=255)

                                                                                       cgcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTg                                                            <  2:333575/150‑1 (MQ=255)

                                                                                       cgcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTg                                                            >  1:1653393/1‑150 (MQ=255)

                                                                                        gcCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAgg                                                                                                                              >  2:675598/1‑83 (MQ=255)

                                                                                           aGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCt                                                        <  2:3612067/150‑1 (MQ=255)

                                                                                                 gcaCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACAccc                                                  <  2:4703333/150‑1 (MQ=255)

                                                                                                  caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACAcccc                                                 <  1:1455224/150‑1 (MQ=255)

                                                                                                  caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACAcccc                                                 <  1:765635/150‑1 (MQ=255)

                                                                                                  caCCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACAcccc                                                 >  2:2494641/1‑150 (MQ=255)

                                                                                                       gcagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCt                                            >  2:3537324/1‑150 (MQ=255)

                                                                                                       gcagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCt                                            >  1:3954752/1‑150 (MQ=255)

                                                                                                        cagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTc                                           <  2:1563632/150‑1 (MQ=255)

                                                                                                        cagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTc                                           <  1:2857890/150‑1 (MQ=255)

                                                                                                        cagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTc                                           <  2:4277391/150‑1 (MQ=255)

                                                                                                        cagcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTc                                           <  1:1273674/150‑1 (MQ=255)

                                                                                                          gcCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGa                                         <  1:4303572/150‑1 (MQ=255)

                                                                                                           cccGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGAc                                        <  2:1308766/150‑1 (MQ=255)

                                                                                                                 gAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTg                                  <  2:2991265/150‑1 (MQ=255)

                                                                                                                 gAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTg                                  <  2:1748249/150‑1 (MQ=255)

                                                                                                                  aaCAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGc                                 <  1:5283938/150‑1 (MQ=255)

                                                                                                                   aCAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCa                                <  1:18425/150‑1 (MQ=255)

                                                                                                                   aCAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCa                                <  1:3873052/150‑1 (MQ=255)

                                                                                                                       cACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaa                            >  1:3255636/1‑150 (MQ=255)

                                                                                                                        aCCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGaaa                           <  2:1726278/150‑1 (MQ=255)

                                                                                                                          cagcagGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGt                         >  2:1890872/1‑150 (MQ=255)

                                                                                                                           agcagGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTc                        <  1:2319147/150‑1 (MQ=255)

                                                                                                                             cagGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGc                      <  1:1045948/150‑1 (MQ=255)

                                                                                                                             cagGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGc                      <  1:3882770/150‑1 (MQ=255)

                                                                                                                             cagGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGAATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGc                      <  2:1318427/150‑1 (MQ=255)

                                                                                                                                gATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGccac                   <  1:2560717/150‑1 (MQ=255)

                                                                                                                                   gAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTc                        >  2:2156868/1‑142 (MQ=255)

                                                                                                                                   gAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTc                        <  1:2156868/142‑1 (MQ=255)

                                                                                                                                     aGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCAccc              >  2:2735089/1‑150 (MQ=255)

                                                                                                                                      ggCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCg             <  1:5153352/150‑1 (MQ=255)

                                                                                                                                      accctggTGCTGCTGCACCTGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGcca                    <  1:2870392/138‑1 (MQ=255)

                                                                                                                                      accctggTGCTGCTGCACCTGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGcca                    >  2:2870392/6‑143 (MQ=255)

                                                                                                                                      accctggTGCTGCTGCACCTGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCg             >  1:972877/6‑150 (MQ=255)

                                                                                                                                      accctggTGCTGCTGCACCTGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCg             >  1:972940/6‑150 (MQ=255)

                                                                                                                                       gCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCGACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACAACCCgg            >  1:5329165/1‑150 (MQ=255)

                                                                                                                                        cctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTgggg                                                                                           <  1:654407/67‑1 (MQ=255)

                                                                                                                                        cctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGc                                                          >  1:2654449/4‑103 (MQ=255)

                                                                                                                                        cctggTGCTGCTGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGc                                                          <  2:2654449/100‑1 (MQ=255)

                                                                                                                                            gTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGt       <  1:117947/150‑1 (MQ=255)

                                                                                                                                                 gcagcaCCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACgct  <  2:3431243/150‑1 (MQ=255)

TCCCAGGTGGTGAGCGGGCAGCCCAGGTGCAGGCACTCCACCGCCAGGCCCCAGGCCAGGACCGGCAAGTGCAGCAACAGGGCAGGGCGCCAGCGCAGCACCAGCAGCCCGCCGAACAGCACCAGCAGGATGAAGGCCAGGTGCAGCAGCACCAGGGTGTCGGCGGCAAGGCGGTAGAGCATGGGTTGGGTGCCCGGGGATTGGGGTTATCCCAGCATAGTTGGGTTGCCTGATCTGGCCTACACCCCTTCCTCGACCATCTGCAGGAAAGTCGCCACCACCCGGCGCGTACGCT  >  NC_002947/2893624‑2893918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`