BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,003,780	C→G	6.7%	A180G (GCC→GGC)	PP_2625 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,003,780	0	C	G	6.7%	639.7 / 26.8	194	A180G (GCC→GGC)	PP_2625	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (88/93); new base G (5/8); total (93/101)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

Only 100 of 197 total aligned reads displayed.

ATCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACATGTC  >  NC_002947/3003632‑3003929

   aGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcggg                                                                                                                                                   <  2:2815628/150‑1 (MQ=255)

      tGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcgg                                                                                                                                                <  2:5229391/150‑1 (MQ=255)

      tGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcgg                                                                                                                                                <  2:2366086/150‑1 (MQ=255)

       gCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                               >  2:518627/1‑150 (MQ=255)

       gCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                               <  2:2976021/150‑1 (MQ=255)

            cAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGTGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTg                                                                                                                                          <  2:842095/150‑1 (MQ=255)

             aGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGc                                                                                                                                         >  2:1772024/1‑150 (MQ=255)

             aGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGc                                                                                                                                         >  1:1294609/1‑150 (MQ=255)

                ccATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgg                                                                                                                                                    <  1:2831774/136‑1 (MQ=255)

                 cATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGc                                                                                                                                     >  2:2709675/1‑150 (MQ=255)

                   tctcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCa                                                                                                                                   <  2:1293885/150‑1 (MQ=255)

                    ctcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACTCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCAt                                                                                                                                  <  1:3251469/150‑1 (MQ=255)

                     tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                                 <  2:4553024/150‑1 (MQ=255)

                     tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                                 >  2:3466174/1‑150 (MQ=255)

                     tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                                 >  1:591018/1‑150 (MQ=255)

                       ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                               <  2:3704194/150‑1 (MQ=255)

                       ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                               <  2:1598205/150‑1 (MQ=255)

                       ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                               <  1:1835191/150‑1 (MQ=255)

                       ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                               <  2:914499/150‑1 (MQ=255)

                       ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                               <  1:3108205/150‑1 (MQ=255)

                       ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                               <  2:1855500/150‑1 (MQ=255)

                            tACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGa                                                                                                                          <  2:780976/150‑1 (MQ=255)

                               aGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCa                                                                                                                       <  1:2048974/150‑1 (MQ=255)

                               aGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCa                                                                                                                       <  1:4838674/150‑1 (MQ=255)

                                  tGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGa                                                                                                                    >  1:1112426/1‑150 (MQ=255)

                                  tGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGAGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGa                                                                                                                    <  2:3865806/150‑1 (MQ=255)

                                   gATTGCCAGGCGTCTGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGaa                                                                                                                   >  1:2711692/1‑150 (MQ=255)

                                   gATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGaa                                                                                                                   >  1:2150826/1‑150 (MQ=255)

                                    aTTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcgg                                                                                                                                                <  2:82136/120‑1 (MQ=255)

                                    aTTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcgg                                                                                                                                                >  1:82136/1‑120 (MQ=255)

                                    aTTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAg                                                                                                                  >  2:4849013/1‑150 (MQ=255)

                                      tGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCt                                                                                                                <  1:3991713/150‑1 (MQ=255)

                                        ccAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGCGGAGGGCTGGTCTACCCCTCAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGa                                                                                                              >  2:3403839/1‑150 (MQ=255)

                                         cAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGAt                                                                                                             >  1:4361132/1‑150 (MQ=255)

                                         cAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGAt                                                                                                             >  1:2126646/1‑150 (MQ=255)

                                          aGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGTCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                            <  1:3278784/150‑1 (MQ=255)

                                          aGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                            <  1:5034801/150‑1 (MQ=255)

                                               tCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGc                                                                                                       >  1:5310272/1‑150 (MQ=255)

                                                  gATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCa                                                                                                    >  2:4633998/1‑150 (MQ=255)

                                                  gATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCa                                                                                                    <  2:3295029/150‑1 (MQ=255)

                                                    tCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                                  <  2:4427622/150‑1 (MQ=255)

                                                    tCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                                  <  1:4374954/150‑1 (MQ=255)

                                                      gAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGt                                                                                                >  2:5058312/1‑150 (MQ=255)

                                                       aGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGc                                                                                                                                             >  1:1334537/1‑104 (MQ=255)

                                                         gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                             <  1:2334039/150‑1 (MQ=255)

                                                         gCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATc                                                                                             <  1:4347699/150‑1 (MQ=255)

                                                           aaGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGa                                                                                           <  1:2750045/150‑1 (MQ=255)

                                                            aGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAg                                                                                          <  1:3644106/150‑1 (MQ=255)

                                                               tGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGtt                                                                                       <  1:338235/150‑1 (MQ=255)

                                                                   tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                                   <  2:2451252/150‑1 (MQ=255)

                                                                     aaGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagt                                                                                 <  2:1437584/150‑1 (MQ=255)

                                                                       ggTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtga                                                                               <  2:2128300/150‑1 (MQ=255)

                                                                         tACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATc                                                                             <  2:387710/150‑1 (MQ=255)

                                                                            gCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACg                                                                          <  2:315037/150‑1 (MQ=255)

                                                                            gCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACg                                                                          >  2:3128379/1‑150 (MQ=255)

                                                                            gCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACg                                                                          >  2:4726572/1‑150 (MQ=255)

                                                                             ccTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACgg                                                                         <  1:644771/150‑1 (MQ=255)

                                                                              cTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGc                                                                        >  1:1082928/1‑150 (MQ=255)

                                                                                     gggAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                                  >  2:4144398/1‑117 (MQ=255)

                                                                                     gggAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                                  <  1:4144398/117‑1 (MQ=255)

                                                                                        tacgCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTa                                                                                               >  2:4359083/4‑117 (MQ=255)

                                                                                          ggCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCCTCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTCTCGAGGGTGTGAGTGATCACGGCGAGGTCGACggg                                                            >  2:5200393/1‑150 (MQ=255)

                                                                                          ggCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACggg                                                            <  2:2711692/150‑1 (MQ=255)

                                                                                           gCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagt                                                                                 >  1:2904356/1‑128 (MQ=255)

                                                                                           gCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagt                                                                                 <  2:2904356/128‑1 (MQ=255)

                                                                                               gTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGAc                                                       >  2:4700658/1‑150 (MQ=255)

                                                                                                  tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCa                                                                                                    >  1:5421688/1‑102 (MQ=255)

                                                                                                  tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCtt                                                    <  1:4408375/150‑1 (MQ=255)

                                                                                                  tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCtt                                                    >  1:429571/1‑150 (MQ=255)

                                                                                                    cccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGGGAGTGATCACGGCGAGGTCGACGGGCTGACCGTCg                                                  >  1:467835/1‑150 (MQ=255)

                                                                                                     ccGTGAGCCGCCCGGGCAAGTGAGGCAGAACGGCCAAGGGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGa                                                 <  1:3788782/150‑1 (MQ=255)

                                                                                                     ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGa                                                 <  1:2788760/150‑1 (MQ=255)

                                                                                                     ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGCCGGGGCCTGCCCGCCATCCAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGCCGAGGTCGACGGGCTGACCTTCGa                                                 >  2:2991849/1‑150 (MQ=255)

                                                                                                      cGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                                <  1:4516742/150‑1 (MQ=255)

                                                                                                      cGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGAt                                                <  2:1082928/150‑1 (MQ=255)

                                                                                                       gTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATg                                               <  1:678402/150‑1 (MQ=255)

                                                                                                        tGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATgc                                              <  2:247545/150‑1 (MQ=255)

                                                                                                              gCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAgg                                        <  2:2318123/150‑1 (MQ=255)

                                                                                                                ccTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGcc                                      >  1:2164498/1‑150 (MQ=255)

                                                                                                                 cTGGCAAGTGAGGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCt                                     <  2:3249288/150‑1 (MQ=255)

                                                                                                                  tGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTg                                    >  2:118910/1‑150 (MQ=255)

                                                                                                                      aaGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGccc                                >  1:4724602/1‑150 (MQ=255)

                                                                                                                          gATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCtt                            >  1:4948678/1‑150 (MQ=255)

                                                                                                                             ggAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGAt                         <  1:5194789/150‑1 (MQ=255)

                                                                                                                               agaACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCa                       >  1:3833240/1‑150 (MQ=255)

                                                                                                                                  aCGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc                    <  2:2666533/150‑1 (MQ=255)

                                                                                                                                      tgatctGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGgc                >  1:2358504/6‑150 (MQ=38)

                                                                                                                                      ccAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGtt                                                                                       <  2:3782267/79‑1 (MQ=255)

                                                                                                                                      ccAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGgc                >  1:1765227/1‑150 (MQ=255)

                                                                                                                                       cAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGgcg               <  1:3770532/150‑1 (MQ=255)

                                                                                                                                          gTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGa            <  1:1782577/150‑1 (MQ=255)

                                                                                                                                             cTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTTCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGc                                  <  1:1648926/125‑1 (MQ=255)

                                                                                                                                             cTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGc                                  >  2:1648926/1‑125 (MQ=255)

                                                                                                                                              tCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca        >  1:5140611/1‑150 (MQ=255)

                                                                                                                                              tCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca        >  2:5404294/1‑150 (MQ=255)

                                                                                                                                              tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca        >  2:4306730/1‑150 (MQ=255)

                                                                                                                                                gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcaca      >  1:5450889/1‑150 (MQ=255)

                                                                                                                                                 aTGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGTTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACAt     <  1:3913083/150‑1 (MQ=255)

                                                                                                                                                 aTGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACAt     >  2:1974623/1‑150 (MQ=255)

                                                                                                                                                    ccGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACATGTc  >  1:4456875/1‑150 (MQ=255)

ATCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACATGTC  >  NC_002947/3003632‑3003929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`