| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 207,265 | C→T | 31.6% | V4257V (GTC→GTT) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 207,265 | 0 | C | T | 31.6% | 18.0 / 11.4 | 22 | V4257V (GTC→GTT) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (10/3); new base T (2/4); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGG > NC_002947/207126‑207311 | cTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAACTCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTGACCGACGTGC < 1:56597‑M1/150‑1 (MQ=255)cTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAACTCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGC < 2:3191901‑M1/150‑1 (MQ=255)cTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGC > 1:4203702‑M1/1‑150 (MQ=255)cTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGC < 2:322631‑M1/150‑1 (MQ=255)cTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGC > 1:3657625‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 1:3792161‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 1:2290245‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 2:4942556‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 2:4246182‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 2:2945970‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 2:3746075‑M1/1‑150 (MQ=255) tGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 1:2284997‑M1/1‑150 (MQ=255) ccccGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCTCGTCGGTGACCGACGTGCtg < 2:653455‑M1/137‑3 (MQ=255) ccccGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCTCGTCGGTGACCGACGTGCtg > 1:653455‑M1/1‑135 (MQ=255) aCGTCGGTACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGACGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCaggtcgccttcgttgcccggcgtacctgg < 1:2514526‑M1/150‑30 (MQ=255) gttggcacCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCaggtcgccttcgttgcccggcgtacctggct < 2:705998‑M1/147‑32 (MQ=255) aaaTCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC > 2:1744289‑M1/1‑95 (MQ=255) aaaTCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCC < 1:1744289‑M1/95‑1 (MQ=255) tcgatcgCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCaggtcgccttcgttgcccggcgtacct > 2:2146154‑M1/1‑92 (MQ=255) tcgatcgCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACACTGGACAAGACCCCGGTCAGCACGTCGGTTACCGACGAGCCaggtcgccttcgttgcccggcgtacct < 1:2146154‑M1/119‑28 (MQ=255) gAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCaggtcgccttcgttgcccggcgtacctggctcgtc > 2:3055602‑M1/1‑53 (MQ=255) gAGCAACTGACGCTGGACAAGACGCCGGTCAGCACGTCGGTTACCGACGAGCCaggtcgccttcgttgcccggcgtacctggctcgtc < 1:3055602‑M1/88‑36 (MQ=255) | CTGCCACTGTCACTGCCCCGGACAACGTCTACGTCGGCACCAACGACCCTGTGATCAAATCGATCGCAACCGTTGAAGGTGCGGATGTCGGCAAGTTCGAGCAACTGACGCTGGACAAGACCCCGGTCAGCACGTCGGTCACCGACGAGCCGGGCACCCCAGGCAACGAAGGCGACTTGGTCAAGG > NC_002947/207126‑207311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |