Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,056,697 C→G 5.9% intergenic (‑207/+101) PP_2666 ← / ← PP_2667 hypothetical protein/ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,056,6970CG 5.9% 274.0 / 6.5 85intergenic (‑207/+101)PP_2666/PP_2667hypothetical protein/ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (34/46);  new base G (3/2);  total (37/48)
Fisher's exact test for biased strand distribution p-value = 6.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

CGACGATAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATC  >  NC_002947/3056549‑3056846
                                                                                                                                                    |                                                                                                                                                      
cgacgaTAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGCGTGCGGCGCGGCAGGGCCgcgcc                                                                                                                                                       >  2:3427032/1‑149 (MQ=38)
 gacgaTAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGGGTGCGGGGCGAAAGGGgcgcgccg                                                                                                                                                      >  1:3612016/1‑148 (MQ=38)
  acgaTAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGc                                                                                                                                                     <  1:2883759/150‑1 (MQ=255)
       aGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGccc                                                                                                                                                <  2:5407525/150‑1 (MQ=255)
        gACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGGGGGCGTCGGGAAGGGGGCGCGCCGCGGcccc                                                                                                                                               >  2:2311048/1‑150 (MQ=37)
           aGTAAATGCAGTACTAAAGTAGTAGATGGTCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCTGCGCGGccctttc                                                                                                                                            <  2:486020/150‑5 (MQ=37)
           aGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGCGGGGGGGCCCCAAGGGCCGCGCCGCCGcccccgc                                                                                                                                            >  2:591531/1‑147 (MQ=25)
               aaTGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCGTGCGGCGCGAAAGGGGCGCCCCCCGGCCCCacgcgcg                                                                                                                                        >  2:5382248/1‑144 (MQ=37)
                aTGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGAtctc                                                                                                                                       <  1:864243/150‑1 (MQ=255)
                 tGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCg                                                                                                                                      <  1:1675166/150‑1 (MQ=255)
                            agtagtAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCAt                                                                                                                           <  1:5283079/150‑1 (MQ=255)
                             gtagtaGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATa                                                                                                                          <  1:3816784/150‑1 (MQ=255)
                                       gCCTGGCGACTGTAGGCTTGTAGACGCCGTAGGGAGAAACTGTATTGCTGAATTTCTGATAGCTGGCGCGATCCCTGTGGGAGCGGACTTGCGTCGCGATAGGGCCGCGCAGCGGCCCCTGGATCTCGGCATCATTCATAGATTGCTggg                                                                                                                <  2:2355845/150‑1 (MQ=25)
                                            gcgcCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGCGAGCGGCGCGCAATGGCCCCCCACCGGCCCCCCCATCGCGGCCGCAAGCAAAGAGTTCAGGGGgcgc                                                                                                           >  2:4077599/1‑150 (MQ=16)
                                             cgcCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCt                                                                                                          <  2:3810334/150‑1 (MQ=255)
                                                 tGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGc                                                                                                      <  2:7002/150‑1 (MQ=255)
                                                 tGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCGGGCGGCGCGCCACGGCCCCGCAGCCGCCCCACGCTCGCGGCCACAAGCAGAGCTTGCTGGGGCCGCGTTGc                                                                                                      >  2:1261693/1‑150 (MQ=21)
                                                  gAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCg                                                                                                     <  1:2301022/150‑1 (MQ=255)
                                                    aGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCACGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGc                                                                                                   <  1:1007165/150‑1 (MQ=255)
                                                     ggCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGcc                                                                                                  <  1:973898/150‑1 (MQ=255)
                                                        aTGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCAAAGCGGcccca                                                                                               <  1:176358/150‑3 (MQ=255)
                                                          ggAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATc                                                                                             <  2:840630/150‑1 (MQ=255)
                                                            agaCGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATcgc                                                                                           <  2:1356993/150‑1 (MQ=255)
                                                               cGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGac                                                                                        <  1:2406086/150‑1 (MQ=255)
                                                                 cccTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGacac                                                                                      <  2:1327869/150‑1 (MQ=255)
                                                                 cccTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGacac                                                                                      <  2:2145862/150‑1 (MQ=255)
                                                                 cccTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGacac                                                                                      <  1:5264718/150‑1 (MQ=255)
                                                                          gCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCt                                                                             <  1:1261693/150‑1 (MQ=255)
                                                                                   ttGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACgg                                                                   <  1:2664957/150‑1 (MQ=255)
                                                                                           tttCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGGCGTTCGGGGCGGAAGGGCCCCGCCGCCGCCCCCCGGTCGCCGCAACATGCAAAAATTGGGGGGGGCGCGTTGGGGGCCCAACCCGACCCAAGGCCCCACCCACCCCGGGTAacac                                                            >  2:4448267/1‑150 (MQ=255)
                                                                                             tCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAgg                                                                                                                                            >  2:3641095/1‑68 (MQ=255)
                                                                                             tCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAgg                                                                                                                                            <  1:3641095/68‑1 (MQ=255)
                                                                                              cTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCa                                                        <  1:4884176/150‑1 (MQ=255)
                                                                                                gAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTggg                                                                                                                <  2:569022/93‑1 (MQ=255)
                                                                                                gAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTggg                                                                                                                >  1:569022/1‑93 (MQ=255)
                                                                                                 aaaGCTGGCGCGATCCCTGTGGTAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGCCCCCAGGATCACGGCATCATCAATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGCCCGCTCCCA‑CACGGGTTACACGCCAGGt                                                     <  1:3723227/150‑1 (MQ=38)
                                                                                                    gCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGtttt                                                  <  2:3768635/150‑1 (MQ=255)
                                                                                                    gCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGtttt                                                  <  2:3767433/150‑1 (MQ=255)
                                                                                                        gcgcGATCCCTGTGGGAGCGGCCGTGCGGCGCGAAAGGGCCGCGCAGCGGCCCCCGGGTCTCGGCGTCAAGCAAAGTTTGGTGGGGGCCCGGTGGGGGCCCAAAGCGACACCAAGCCCCGCCCA‑CACCGGGTAGACGCCCGGGTTTGAgc                                              >  2:5000089/1‑150 (MQ=255)
                                                                                                                    tgGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCa                                  <  1:1739402/150‑1 (MQ=255)
                                                                                                                          cGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTg                            >  2:5224739/1‑150 (MQ=255)
                                                                                                                           ggCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGc                           >  2:4734160/1‑150 (MQ=255)
                                                                                                                           ggCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAAACGCGACACAAGGCCGCTCCCA‑CACGGGTTACACCCCAGGTTTTAAGCGCGGCTTGCgcagcgcagc                           >  1:5073725/1‑150 (MQ=255)
                                                                                                                            gCCGTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGGGTTCGGCGCGGGTTGCGCCGCGCTGCa                          >  1:2367150/1‑150 (MQ=37)
                                                                                                                             ccTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCAt                         >  2:3586936/1‑150 (MQ=255)
                                                                                                                             ccTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCAt                         >  1:1631366/1‑150 (MQ=255)
                                                                                                                             ccTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCAt                         >  2:4402497/1‑150 (MQ=255)
                                                                                                                              cTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAAACGCGACACAAGGCCGCTCCCA‑CACGGGTTACACCCCGGGTTTTCAGCGCGGCTGGCGCAGCGCTGCATg                        >  1:627769/1‑150 (MQ=255)
                                                                                                                               ttGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGc                       >  2:885462/1‑150 (MQ=255)
                                                                                                                               ttGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGc                       >  1:5445214/1‑150 (MQ=255)
                                                                                                                                 gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTg                     >  1:39280/1‑150 (MQ=255)
                                                                                                                                 gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTg                     <  1:804855/150‑1 (MQ=255)
                                                                                                                                 gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTg                     >  2:2612339/1‑150 (MQ=255)
                                                                                                                                 gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTg                     >  2:2611919/1‑150 (MQ=255)
                                                                                                                                 gCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCAAAGATTGCTGGGGCCGCTTAGCGGCCCAATCGCGACACAAGGCCGCTTCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGAGCTGCatgatg                     >  2:711665/1‑150 (MQ=38)
                                                                                                                                   gTCGCGAACGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggg                   <  1:4676270/150‑1 (MQ=255)
                                                                                                                                   gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGTTTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggg                   <  1:1601883/150‑1 (MQ=255)
                                                                                                                                   gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggg                   >  1:3750092/1‑150 (MQ=255)
                                                                                                                                   gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggg                   >  1:4127532/1‑150 (MQ=255)
                                                                                                                                   gTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggg                   >  1:4127714/1‑150 (MQ=255)
                                                                                                                                    tCGCGCACGGGCCGCGCAGCGGCCCCAGGACCTCGGCATCTTGCACAGATTGCGGGGGCCGCTGTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACCCGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTgggg                  <  1:1081389/150‑1 (MQ=37)
                                                                                                                                    tCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGTTTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTgggg                  <  1:4992818/150‑1 (MQ=255)
                                                                                                                                    tCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTgggg                  <  2:3132134/150‑1 (MQ=255)
                                                                                                                                     cgcgACAGGGCCGCGCAGCGCCCCCAGCACCTCGGCATCATGCAAAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACACGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTggggg                 <  1:2791562/150‑1 (MQ=38)
                                                                                                                                      gcgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGt                >  2:1632239/1‑150 (MQ=255)
                                                                                                                                       cgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGtt               >  1:4449943/1‑150 (MQ=255)
                                                                                                                                       cgAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGAGTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGtt               <  2:1290170/150‑1 (MQ=255)
                                                                                                                                        gAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTg              >  1:5201115/1‑150 (MQ=255)
                                                                                                                                         ctggggCCGCTGCGCGGCCCTAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGa             >  1:2395019/4‑150 (MQ=38)
                                                                                                                                          tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGccc                                                                                                 <  1:5425941/64‑1 (MQ=37)
                                                                                                                                          tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGccc                                                                                                 >  2:5425941/3‑66 (MQ=37)
                                                                                                                                          tggggCCGCTGCGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGaa            >  1:3017255/3‑150 (MQ=255)
                                                                                                                                          aaGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGaa            <  2:3994997/150‑1 (MQ=255)
                                                                                                                                             ggCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGt         <  2:5438155/150‑1 (MQ=255)
                                                                                                                                             ggCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGt         <  1:5382248/150‑1 (MQ=255)
                                                                                                                                              gccgcgccgcGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTg        <  1:1608046/150‑1 (MQ=255)
                                                                                                                                              gCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTg        <  1:1608155/150‑1 (MQ=255)
                                                                                                                                              gCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTg        >  2:427133/1‑150 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       <  2:3606818/150‑1 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       <  1:5391739/150‑1 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       >  1:1821159/1‑150 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       <  1:2612403/150‑1 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       <  2:4842777/150‑1 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       >  1:3898490/1‑150 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       >  2:3383648/1‑150 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       >  1:4071905/1‑150 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       <  2:3439359/150‑1 (MQ=255)
                                                                                                                                               ccGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGa       <  2:3612016/150‑1 (MQ=255)
                                                                                                                                                 gcgcAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAgg     >  2:5025419/1‑150 (MQ=255)
                                                                                                                                                 gcgcAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAgg     <  1:2311048/150‑1 (MQ=255)
                                                                                                                                                 gcgcAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAgg     >  2:2569096/1‑150 (MQ=255)
                                                                                                                                                 gcgcAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAgg     >  2:845434/1‑150 (MQ=255)
                                                                                                                                                   gcAGCGGCCCCAGGATCTCGGCATCATGCATAGATGGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGAt   <  2:1187995/150‑1 (MQ=255)
                                                                                                                                                    cAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATc  <  2:4077244/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CGACGATAGACAGTAAATGCAGTACTAAAGTAGTAGATGGCCGGGCGCCTGAAGGCATGGAGACGCCCTAGGGAGCAACTGTCTTGCTCAATTTCTGAAAGCTGGCGCGATCCCTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGCGCAGCGGCCCCAGGATCTCGGCATCATGCATAGATTGCTGGGGCCGCTTTGCGGCCCAATCGCGACACAAGGCCGCTCCCA‑CACGGGTTACACGCCAGGTTTTCAGCGCGGCTTGCGCAGCGCTGCATGCTGGGGGTTGAAGGTGAGGATC  >  NC_002947/3056549‑3056846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑