Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,072,452 C→T 7.7% intergenic (+55/‑299) aldB‑II → / → pqqD‑II aldehyde dehydrogenase/coenzyme PQQ synthesis protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,072,4520CT 7.7% 439.7 / 18.1 144intergenic (+55/‑299)aldB‑II/pqqD‑IIaldehyde dehydrogenase/coenzyme PQQ synthesis protein D
Reads supporting (aligned to +/- strand):  ref base C (85/47);  new base T (4/7);  total (90/54)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 149 total aligned reads displayed.

TTGGCCGTGAGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCC  >  NC_002947/3072303‑3072601
                                                                                                                                                     |                                                                                                                                                     
 tGGCCGTGAGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCa                                                                                                                                                      <  1:145837/150‑1 (MQ=255)
 tGGCCGTGAGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCa                                                                                                                                                      <  1:146116/150‑1 (MQ=255)
 tGGCCGTGAGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCa                                                                                                                                                      >  1:4064409/1‑150 (MQ=255)
     cGTGAGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAAc                                                                                                                                                  <  2:5187869/150‑1 (MQ=255)
         agaCCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGt                                                                                                                                              <  2:3712021/150‑1 (MQ=255)
          gaCCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTg                                                                                                                                             >  1:3441105/1‑150 (MQ=255)
           aCCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAAccagcgg                                                                                                                                            <  2:4513186/150‑6 (MQ=18)
           aCCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTga                                                                                                                                            >  1:4265055/1‑150 (MQ=255)
              cacaAGATGATGCTTGACCATTATCCGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGGTCCAGAACCGGTgaaga                                                                                                                                         >  2:4207174/1‑150 (MQ=255)
              cacaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaaga                                                                                                                                         >  1:3529717/1‑150 (MQ=255)
              cacaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaaga                                                                                                                                         >  2:1460873/1‑150 (MQ=255)
               acaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGACCCGGTgaagac                                                                                                                                        >  2:4422034/1‑149 (MQ=255)
               acaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaagaa                                                                                                                                        >  2:783214/1‑150 (MQ=255)
                caAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAAc                                                                                                                                       <  1:4470849/150‑1 (MQ=255)
                   gatgatGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCtg                                                                                                                                    >  1:74937/1‑150 (MQ=255)
                    atgatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCtgt                                                                                                                                   <  1:2234832/150‑1 (MQ=18)
                      gatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTgg                                                                                                                                 >  1:543506/1‑150 (MQ=255)
                        tgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTggtg                                                                                                                               <  1:340494/150‑3 (MQ=255)
                        tgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTgggg                                                                                                                               >  1:2098407/1‑150 (MQ=255)
                           ttGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcg                                                                                                                            >  1:5043949/1‑150 (MQ=255)
                           ttGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcg                                                                                                                            >  2:3806607/1‑150 (MQ=255)
                           ttGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcg                                                                                                                            >  1:1654010/1‑150 (MQ=255)
                            tGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcgg                                                                                                                           <  2:2740911/150‑1 (MQ=255)
                              aCCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcgggc                                                                                                                         <  1:1236059/150‑1 (MQ=255)
                                cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                       <  2:4074700/150‑1 (MQ=255)
                                cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                       >  1:812229/1‑150 (MQ=255)
                                cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                       <  2:220841/150‑1 (MQ=255)
                                cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCAGTGGGGGCGGGCGa                                                                                                                       <  2:2490645/150‑1 (MQ=255)
                                   tATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGTACCTGTGGGGGCGGGCGAGcc                                                                                                                    >  2:4323483/1‑150 (MQ=255)
                                    aTCAGCAGACCACGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGccc                                                                                                                   <  1:1051661/150‑1 (MQ=255)
                                    aTCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGccc                                                                                                                   >  1:2697839/1‑150 (MQ=255)
                                    aTCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCACTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGccc                                                                                                                   <  2:4271156/150‑1 (MQ=255)
                                     tCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAACCCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCg                                                                                                                  <  2:5359744/150‑1 (MQ=255)
                                        gcagACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCaa                                                                                                               >  2:5012285/1‑150 (MQ=255)
                                           gACCACGAACCTGCTGGTGAGCTACGACATCACTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAg                                                                                                            <  2:2848164/150‑1 (MQ=255)
                                            aCCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAgg                                                                                                           >  1:49293/1‑150 (MQ=255)
                                              cAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGc                                                                                                         >  2:1441611/1‑150 (MQ=255)
                                                  aaCCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGc                                                                                                     >  2:757094/1‑150 (MQ=255)
                                                     ctgctgGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGcaca                                                                                                  >  1:4231364/1‑150 (MQ=255)
                                                            tGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                           >  1:4551305/1‑150 (MQ=255)
                                                            tGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                           >  2:5233585/1‑150 (MQ=255)
                                                            tGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                           >  2:3958683/1‑150 (MQ=255)
                                                               gCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCt                                                                                        >  1:509411/1‑150 (MQ=255)
                                                                 tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAAccag                                                                                                                                               >  2:1371951/1‑91 (MQ=18)
                                                                 tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAACGGGCCGGCGCATGCATGGCCTAc                                                                                      >  2:2923786/1‑150 (MQ=255)
                                                                 tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAc                                                                                      <  1:1106229/150‑1 (MQ=255)
                                                                    gACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCg                                                                                   >  1:1979512/1‑150 (MQ=255)
                                                                      cATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                           <  1:4855396/140‑1 (MQ=255)
                                                                      cATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTa                                                                                 <  1:765605/150‑1 (MQ=255)
                                                                       aTCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTAc                                                                                >  2:3580939/1‑150 (MQ=255)
                                                                       aTCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTAc                                                                                <  2:3563088/150‑1 (MQ=255)
                                                                          aaTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCaa                                                                             >  1:5411320/1‑150 (MQ=255)
                                                                          aaTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCaa                                                                             <  2:2741039/150‑1 (MQ=255)
                                                                           aTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAAc                                                                            <  2:5424819/150‑1 (MQ=255)
                                                                               gCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCa                                                                              >  2:5254926/1‑144 (MQ=255)
                                                                               gCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCa                                                                              <  1:5254926/144‑1 (MQ=255)
                                                                                  gggCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCAcccc                                                                     <  2:646781/150‑1 (MQ=255)
                                                                                       tcttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCacg                                                                >  2:4425458/1‑148 (MQ=255)
                                                                                       tcttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAg                                                                >  2:452985/1‑150 (MQ=255)
                                                                                         ttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCCTGGCCTACCCGTACCAACGCACCCCGCAAGCt                                                              >  1:938974/1‑150 (MQ=255)
                                                                                         ttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCt                                                              <  2:3832944/150‑1 (MQ=255)
                                                                                          tctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTc                                                             >  1:637985/1‑150 (MQ=255)
                                                                                            tAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGt                                                           >  1:1279180/1‑150 (MQ=255)
                                                                                            tAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGt                                                           >  1:2367029/1‑150 (MQ=255)
                                                                                            tAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGt                                                           >  1:3417166/1‑150 (MQ=255)
                                                                                                 cgcgTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCaa                                                      >  2:5131145/1‑150 (MQ=255)
                                                                                                     tCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                  >  2:3313141/1‑150 (MQ=255)
                                                                                                     tCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                  <  2:1302080/150‑1 (MQ=255)
                                                                                                       gcgGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCCGTGAAGAACCGGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGc                                                >  1:1062567/1‑150 (MQ=255)
                                                                                                        cgGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCa                                               >  1:4893098/1‑150 (MQ=255)
                                                                                                          gCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCGGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCAtt                                             >  1:1526214/1‑150 (MQ=255)
                                                                                                            ttttgcgGGCTCGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGGAGCGGGCAAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCCAGCTCGTTCCAAAGTAGCATTCg                                           >  1:3807684/5‑150 (MQ=14)
                                                                                                            cTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGCGCCCGCACAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCg                                           >  1:2392426/1‑150 (MQ=255)
                                                                                                            cTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAg                                                                                                            <  1:3305283/85‑1 (MQ=255)
                                                                                                            cTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAg                                                                                                            >  2:3305283/1‑85 (MQ=255)
                                                                                                               cgcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTc                                        <  1:517952/150‑1 (MQ=255)
                                                                                                               cgcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTc                                        <  1:3023815/150‑1 (MQ=255)
                                                                                                                gcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGATCCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                           <  1:768515/98‑1 (MQ=255)
                                                                                                                gcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGATCCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                           >  2:768515/1‑98 (MQ=255)
                                                                                                                 cgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCa                                      <  1:5012285/150‑1 (MQ=255)
                                                                                                                      ttGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCg                                 >  1:210734/1‑150 (MQ=255)
                                                                                                                          ccGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGcccc                             >  2:3668268/1‑150 (MQ=255)
                                                                                                                            gCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCg                           >  2:2248423/1‑150 (MQ=255)
                                                                                                                                ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCAcatgc                                                                                               >  1:2037614/1‑78 (MQ=255)
                                                                                                                                ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCAcatgc                                                                                               <  2:2037614/78‑1 (MQ=255)
                                                                                                                                ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCa                       >  1:4022390/1‑150 (MQ=255)
                                                                                                                                ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCa                       >  1:1804037/1‑150 (MQ=255)
                                                                                                                                  acaGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                     >  1:4622014/1‑150 (MQ=255)
                                                                                                                                  acaGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                     >  1:4517522/1‑150 (MQ=255)
                                                                                                                                  acaGGTTCACCGCTGGTTCCAGAACCGGCGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                     >  1:313470/1‑150 (MQ=255)
                                                                                                                                    aGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTaa                   >  2:1593022/1‑150 (MQ=255)
                                                                                                                                    aGGTTCACCGCTGGTTCAAGAACCGGTGAAGAACCGGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGAACCAACGCACCCCGAAAGCTCGATCCAAAGTAGCATTCTGTCAAGTACGCCCCCGTGCATTaa                   >  1:1899787/1‑150 (MQ=255)
                                                                                                                                      gTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATg                 >  1:2561609/1‑150 (MQ=255)
                                                                                                                                      gTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATg                 >  1:2896537/1‑150 (MQ=255)
                                                                                                                                       ttCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGc                >  2:4018379/1‑150 (MQ=255)
                                                                                                                                        tCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCt               >  1:679946/1‑150 (MQ=255)
                                                                                                                                             gCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTAc          <  2:543506/150‑1 (MQ=255)
                                                                                                                                               tGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCg        <  1:3647964/150‑1 (MQ=255)
                                                                                                                                                     cAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                  >  1:2283395/1‑102 (MQ=255)
                                                                                                                                                     cAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcc  >  2:2313840/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGGCCGTGAGACCCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCC  >  NC_002947/3072303‑3072601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑