Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,072,464 A→T 5.7% intergenic (+67/‑287) aldB‑II → / → pqqD‑II aldehyde dehydrogenase/coenzyme PQQ synthesis protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,072,4640AT 5.7% 467.9 / 12.9 140intergenic (+67/‑287)aldB‑II/pqqD‑IIaldehyde dehydrogenase/coenzyme PQQ synthesis protein D
Reads supporting (aligned to +/- strand):  ref base A (82/50);  new base T (3/5);  total (85/55)
Fisher's exact test for biased strand distribution p-value = 2.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

Only 100 of 149 total aligned reads displayed.

CCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCACAA  >  NC_002947/3072316‑3072611
                                                                                                                                                    |                                                                                                                                                   
ccACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaag                                                                                                                                                    <  1:1948601/150‑1 (MQ=255)
 cacaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaaga                                                                                                                                                   >  1:3529717/1‑150 (MQ=255)
 cacaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaaga                                                                                                                                                   >  2:1460873/1‑150 (MQ=255)
  acaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGACCCGGTgaagac                                                                                                                                                  >  2:4422034/1‑149 (MQ=255)
  acaAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTgaagaa                                                                                                                                                  >  2:783214/1‑150 (MQ=255)
   caAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAAc                                                                                                                                                 <  2:4027939/150‑1 (MQ=255)
      gatgatGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCtg                                                                                                                                              >  1:75061/1‑150 (MQ=255)
       atgatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCtgt                                                                                                                                             <  1:2234832/150‑1 (MQ=18)
        tgatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCtgtg                                                                                                                                            <  1:407889/150‑1 (MQ=18)
        tgatgCTTGACAATTATCAGCAGACCAAGCACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCtgtg                                                                                                                                            <  2:3848285/150‑1 (MQ=18)
         gatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTgg                                                                                                                                           >  1:4453507/1‑150 (MQ=255)
         gatgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTgg                                                                                                                                           >  1:543506/1‑150 (MQ=255)
           tgCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTgggg                                                                                                                                         >  1:2098407/1‑150 (MQ=255)
              ttGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcg                                                                                                                                      >  2:3806607/1‑150 (MQ=255)
              ttGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCAGTGGgggcg                                                                                                                                      >  1:2490645/1‑150 (MQ=255)
               tGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcgg                                                                                                                                     <  2:2740911/150‑1 (MQ=255)
                 aCCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGgggcgggc                                                                                                                                   <  1:1236059/150‑1 (MQ=255)
                   cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                                 >  1:812229/1‑150 (MQ=255)
                   cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                                 <  2:4074700/150‑1 (MQ=255)
                   cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGa                                                                                                                                 <  2:220841/150‑1 (MQ=255)
                   cATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCAGTGGGGGCGGGCGa                                                                                                                                 <  2:2490645/150‑1 (MQ=255)
                     ttATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGc                                                                                                                               <  1:4748434/150‑1 (MQ=255)
                      tATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGTACCTGTGGGGGCGGGCGAGcc                                                                                                                              >  2:4323483/1‑150 (MQ=255)
                      tATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTT‑GCGGGCTCGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGGAGCGGGCAAGccc                                                                                                                             <  1:5341596/150‑1 (MQ=17)
                       aTCAGCAGACCACGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGccc                                                                                                                             <  1:1051661/150‑1 (MQ=255)
                       aTCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCACTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGccc                                                                                                                             <  2:4271156/150‑1 (MQ=255)
                        tCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAACCCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCg                                                                                                                            <  2:5359744/150‑1 (MQ=255)
                           gcagACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCaa                                                                                                                         >  2:5012285/1‑150 (MQ=255)
                           gcagACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCaa                                                                                                                         >  1:2741039/1‑150 (MQ=255)
                            cagACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCaaa                                                                                                                        >  1:2289103/1‑150 (MQ=255)
                            cagACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCaaa                                                                                                                        >  1:55805/1‑150 (MQ=255)
                              gACCACGAACCTGCTGGTGAGCTACGACATCACTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAg                                                                                                                      <  2:2848164/150‑1 (MQ=255)
                                 cAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGc                                                                                                                   >  2:1441611/1‑150 (MQ=255)
                                 cAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGc                                                                                                                   >  1:3832944/1‑150 (MQ=255)
                                    gAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCgg                                                                                                                >  1:2047186/1‑150 (MQ=255)
                                     aaCCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGc                                                                                                               >  2:757094/1‑150 (MQ=255)
                                        ctgctgGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGcaca                                                                                                            >  1:4546806/1‑150 (MQ=255)
                                            tgGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCAcatgca                                                                                                        >  2:765605/1‑150 (MQ=255)
                                               tGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                                     >  2:5233585/1‑150 (MQ=255)
                                               tGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                                     >  2:3958683/1‑150 (MQ=255)
                                                  gCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCt                                                                                                  >  1:509411/1‑150 (MQ=255)
                                                    tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAACGGGCCGGCGCATGCATGGCCTAc                                                                                                >  2:2923786/1‑150 (MQ=255)
                                                    tACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAc                                                                                                <  1:1106229/150‑1 (MQ=255)
                                                    tACGACATCAATCCGCTGGGCTTCTGCGAAACCGCGTCGCGGCCTACGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACATGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTAc                                                                                                >  2:524036/1‑150 (MQ=255)
                                                       gACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCg                                                                                             >  1:1979512/1‑150 (MQ=255)
                                                         cATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTa                                                                                           <  1:765605/150‑1 (MQ=255)
                                                          aTCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTAc                                                                                          >  2:3580941/1‑150 (MQ=255)
                                                          aTCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTAc                                                                                          >  2:3580939/1‑150 (MQ=255)
                                                             aaTCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCaa                                                                                       <  2:2741039/150‑1 (MQ=255)
                                                                 cGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCa                                                                                   >  1:2197129/1‑150 (MQ=255)
                                                                  gCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCa                                                                                        <  1:5254926/144‑1 (MQ=255)
                                                                          tcttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCacg                                                                          >  2:4425458/1‑148 (MQ=255)
                                                                          tcttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAg                                                                          >  2:452985/1‑150 (MQ=255)
                                                                            ttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCCTGGCCTACCCGTACCAACGCACCCCGCAAGCt                                                                        >  1:938974/1‑150 (MQ=255)
                                                                            ttctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCt                                                                        >  1:1482029/1‑150 (MQ=255)
                                                                             tctAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTc                                                                       >  1:637985/1‑150 (MQ=255)
                                                                               tAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGt                                                                     >  1:2367029/1‑150 (MQ=255)
                                                                               tAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGt                                                                     >  1:1279180/1‑150 (MQ=255)
                                                                               tAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGt                                                                     >  1:3417166/1‑150 (MQ=255)
                                                                                    cgcgTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCaa                                                                >  2:5131145/1‑150 (MQ=255)
                                                                                        tCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                            >  2:3313141/1‑150 (MQ=255)
                                                                                        tCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTa                                                            <  2:1302080/150‑1 (MQ=255)
                                                                                          gcgGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCCGTGAAGAACCGGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGc                                                          >  1:1062567/1‑150 (MQ=255)
                                                                                           cgGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCa                                                         >  1:4893098/1‑150 (MQ=255)
                                                                                             gCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCGGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCAtt                                                       >  1:1526214/1‑150 (MQ=255)
                                                                                               ttttgcgGGCTCGCCCGCCCCCACAGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGGAGCGGGCAAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCCAGCTCGTTCCAAAGTAGCATTCg                                                     >  1:3807684/5‑150 (MQ=14)
                                                                                               cTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAg                                                                                                                      <  1:3305283/85‑1 (MQ=255)
                                                                                               cTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAg                                                                                                                      >  2:3305283/1‑85 (MQ=255)
                                                                                                  cgcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTc                                                  <  1:5115669/150‑1 (MQ=255)
                                                                                                   gcgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGATCCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATgg                                                                                                     <  1:768515/98‑1 (MQ=255)
                                                                                                    cgGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCa                                                <  1:5012285/150‑1 (MQ=255)
                                                                                                         ttGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCCTGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTCGCATTCGGTCCAGTCCg                                           >  2:4243687/1‑150 (MQ=255)
                                                                                                          tGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGc                                          <  1:4929146/150‑1 (MQ=255)
                                                                                                              cGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGccccc                                      >  2:5379792/1‑150 (MQ=255)
                                                                                                               gCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCg                                     >  2:2248423/1‑150 (MQ=255)
                                                                                                                   ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCAcatgc                                                                                                         <  2:2037614/78‑1 (MQ=255)
                                                                                                                   ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCGTTCGGTCCAGTCCGCCCCCGTGCa                                 >  1:3347065/1‑150 (MQ=255)
                                                                                                                   ccACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCa                                 >  1:1804037/1‑150 (MQ=255)
                                                                                                                     acaGGTTCTTCACCGGTTCTGGAACCAGCGGTGAACCTGTGGGAGCGGGCAAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                               >  1:16925/1‑150 (MQ=21)
                                                                                                                     acaGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                               >  2:377314/1‑150 (MQ=255)
                                                                                                                     acaGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                               >  1:4622014/1‑150 (MQ=255)
                                                                                                                     acaGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                               >  1:4517522/1‑150 (MQ=255)
                                                                                                                     acaGGTTCACCGCTGGTTCCAGAACCGGCGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCAtt                               >  1:313470/1‑150 (MQ=255)
                                                                                                                       aGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTaa                             >  2:1593022/1‑150 (MQ=255)
                                                                                                                         gTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATg                           >  1:2561609/1‑150 (MQ=255)
                                                                                                                         gTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATg                           >  1:2896537/1‑150 (MQ=255)
                                                                                                                         gTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATg                           >  1:3026447/1‑150 (MQ=255)
                                                                                                                           tCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCt                         >  1:679946/1‑150 (MQ=255)
                                                                                                                               cGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTa                     <  1:5027739/150‑1 (MQ=255)
                                                                                                                                gCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTAc                    <  2:543506/150‑1 (MQ=255)
                                                                                                                                        cAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcc            >  2:588828/1‑150 (MQ=255)
                                                                                                                                        cAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcc            >  2:2313840/1‑150 (MQ=255)
                                                                                                                                          gAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcctc          <  2:4551305/150‑1 (MQ=255)
                                                                                                                                          gAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAAtcctc          <  2:4231364/150‑1 (MQ=255)
                                                                                                                                            aCCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCg        <  2:3529717/150‑1 (MQ=255)
                                                                                                                                             ccGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCgg       >  2:3432578/1‑150 (MQ=255)
                                                                                                                                             ccGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCAGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCgg       <  2:812229/150‑1 (MQ=255)
                                                                                                                                                gTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGcac    <  2:1246652/150‑1 (MQ=255)
                                                                                                                                                 tGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGcaca   >  1:4376119/1‑150 (MQ=255)
                                                                                                                                                  gaagaaCCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCACaa  <  1:4207174/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CCACAAGATGATGCTTGACCATTATCAGCAGACCAAGAACCTGCTGGTGAGCTACGACATCAATCCGCTGGGCTTCTTCTAAACCGCGTCGCGGCCTTCGCGGGCTTGCCCGCTCCCACAGGTTCACCGCTGGTTCCAGAACCGGTGAAGAACCTGTGGGGGCGGGCGAGCCCGCAAAAGGGCCGGCACATGCATGGCCTACCCGTACCAACGCACCCCGCAAGCTCGTTCCAAAGTAGCATTCGGTCCAGTCCGCCCCCGTGCATTAATGCTTCTACCGGAATCCTCCGGCACAA  >  NC_002947/3072316‑3072611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑