BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,168,021	G→C	5.3%	G163A (GGC→GCC)	PP_2780 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,168,021	0	G	C	5.3%	696.7 / 20.6	208	G163A (GGC→GCC)	PP_2780	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand): ref base G (108/88); new base C (8/3); total (116/92)
Fisher's exact test for biased strand distribution p-value = 3.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 218 total aligned reads displayed.

TCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACACCA  >  NC_002947/3167872‑3168170

tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCACCGACCGCCTTGCGCGGGTGATTGCCGATgg                                                                                                                                                       >  2:59393/1‑150 (MQ=255)

tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATgg                                                                                                                                                       >  2:3398629/1‑150 (MQ=255)

tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATgg                                                                                                                                                       >  2:2553844/1‑150 (MQ=255)

tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATgg                                                                                                                                                       >  2:5242830/1‑150 (MQ=255)

tCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGACgg                                                                                                                                                       >  1:751020/1‑150 (MQ=255)

         gCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAg                                                                                                                                              >  2:4274121/1‑150 (MQ=255)

          cAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGt                                                                                                                                             >  1:3352828/1‑150 (MQ=255)

          cAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGCCATCGGCaat                                                                                                                                             <  2:3924017/150‑3 (MQ=255)

           aGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTg                                                                                                                                            >  1:2418216/1‑150 (MQ=255)

                   ctgctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCg                                                                                                                                    <  1:5110969/150‑1 (MQ=255)

                    tgctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCgg                                                                                                                                   <  2:2611124/150‑1 (MQ=255)

                    tgctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCgg                                                                                                                                   <  1:3732814/150‑1 (MQ=255)

                     gctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGt                                                                                                                                  <  2:4601849/150‑1 (MQ=255)

                     gctCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGt                                                                                                                                  <  1:1268285/150‑1 (MQ=255)

                       tCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                                >  2:1243557/1‑150 (MQ=255)

                       tCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGa                                                                                                                                >  2:2383906/1‑150 (MQ=255)

                          gCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCa                                                                                                                             <  2:3352828/150‑1 (MQ=255)

                           ccTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCaa                                                                                                                            >  1:5323164/1‑150 (MQ=255)

                            cTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAAt                                                                                                                           <  2:1909312/150‑1 (MQ=255)

                            cTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAAt                                                                                                                           <  1:1846756/150‑1 (MQ=255)

                             tGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATg                                                                                                                          >  2:3628241/1‑150 (MQ=255)

                                       cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                                >  2:2746765/1‑150 (MQ=255)

                                       cccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAgcgc                                                                                                                >  1:1225704/1‑150 (MQ=255)

                                        ccGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGcc                                                                                                               >  2:436415/1‑150 (MQ=255)

                                          ggCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTg                                                                                                             >  1:3159151/1‑150 (MQ=255)

                                             gcAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACTGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTgcgc                                                                                                          <  2:1367847/150‑1 (MQ=255)

                                             gcAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTgcgc                                                                                                          >  2:2864291/1‑150 (MQ=255)

                                             gcAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTgcgc                                                                                                          >  1:1933477/1‑150 (MQ=255)

                                               ctgcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAgg                                                                                                                                        <  1:1014705/116‑1 (MQ=255)

                                               aGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCgg                                                                                                        >  2:2687989/1‑150 (MQ=255)

                                               aGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCgg                                                                                                        <  1:1936603/150‑1 (MQ=255)

                                                 gcgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGa                                                                                                      <  2:612877/150‑1 (MQ=255)

                                                 gcgcAGTCCTTGTCTCTATTGTTTGCGAGACAGTTGCAGGTCGATCCTGAATGTTAACTGATTGAGTGCAACGACCGCCTTGCGCGGGTGATTGCATATGTCATCGTCAGTGTAGGCCCGTTTATAAATGTCTGCAGCGCCTgcggtgga                                                                                                      <  2:3138446/150‑6 (MQ=255)

                                                  cgcCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAg                                                                                                     <  2:5141854/150‑1 (MQ=255)

                                                     cGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGt                                                                                                  >  1:3023314/1‑150 (MQ=255)

                                                         cTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCTTCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCa                                                                                              <  1:1266005/150‑1 (MQ=255)

                                                          tGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAg                                                                                             >  2:2303471/1‑150 (MQ=255)

                                                          tGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAg                                                                                             <  1:3996514/150‑1 (MQ=255)

                                                          tGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAg                                                                                             >  2:4877442/1‑150 (MQ=255)

                                                           gggCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACGGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAgg                                                                                            <  1:1816112/150‑1 (MQ=255)

                                                             gCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGcc                                                                                          >  2:4984300/1‑150 (MQ=255)

                                                              cTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCa                                                                                         <  2:5400872/150‑1 (MQ=255)

                                                                 aaTTGATGGTGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                                      >  2:1806195/1‑150 (MQ=255)

                                                                 aaTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCg                                                                                      >  2:1020594/1‑150 (MQ=255)

                                                                  aTTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCgg                                                                                     >  1:603275/1‑150 (MQ=255)

                                                                     gATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCaa                                                                                  >  2:2789616/1‑150 (MQ=255)

                                                                        ggCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGc                                                                               <  1:3086581/150‑1 (MQ=255)

                                                                           gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCACTGCAtt                                                                            >  1:5161206/1‑150 (MQ=255)

                                                                           gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                            >  1:3645330/1‑150 (MQ=255)

                                                                           gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                            >  1:889465/1‑150 (MQ=255)

                                                                           gcgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                            <  2:2630687/150‑1 (MQ=255)

                                                                            cgACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTc                                                                           >  2:4448930/1‑150 (MQ=255)

                                                                              aCAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                         <  1:4739715/150‑1 (MQ=255)

                                                                              aCAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                         <  1:4448930/150‑1 (MQ=255)

                                                                              aCAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                         <  1:564051/150‑1 (MQ=255)

                                                                                aGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAga                                                                       <  1:2303647/150‑1 (MQ=255)

                                                                                  gcgcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCggg                                                                                                       <  1:2623372/116‑1 (MQ=255)

                                                                                  gcgcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgatg                                                                     >  2:3249675/1‑150 (MQ=255)

                                                                                    gcAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAgatgat                                                                   <  1:2270325/150‑1 (MQ=255)

                                                                                      aGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCa                                                                         <  2:1684615/142‑1 (MQ=255)

                                                                                       ggTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCg                                                                <  2:3785496/150‑1 (MQ=255)

                                                                                              ccTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCgcggc                                                         <  1:2774465/150‑1 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGa                                                                                                                                           >  2:1940490/1‑66 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGa                                                       <  2:4186891/150‑1 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGa                                                       >  1:69685/1‑150 (MQ=255)

                                                                                                tGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGa                                                       >  1:1974844/1‑150 (MQ=255)

                                                                                                 gACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGt                                                                                                                         <  1:4003407/83‑1 (MQ=255)

                                                                                                 gACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGt                                                                                                                         >  2:4003407/1‑83 (MQ=255)

                                                                                                 gACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAg                                                      >  1:1697527/1‑150 (MQ=255)

                                                                                                    aGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTc                                                   >  1:1317869/1‑150 (MQ=255)

                                                                                                      atCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGGGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCAGCGGCCCTGCAATCCAGATGATCCGTCGCGGCGAGGTCgc                                                 >  2:2117460/2‑149 (MQ=255)

                                                                                                         aCTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAtt                                                                            >  1:415790/1‑120 (MQ=255)

                                                                                                         aCTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCt                                              >  1:2256767/1‑150 (MQ=255)

                                                                                                          cTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTg                                             <  2:871263/150‑1 (MQ=255)

                                                                                                          cTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTg                                             >  2:2336015/1‑150 (MQ=255)

                                                                                                           tGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTgg                                            >  2:448050/1‑150 (MQ=255)

                                                                                                               tCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCAt                                        >  2:5091467/1‑150 (MQ=255)

                                                                                                                 agagCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTg                                      >  2:4762655/1‑150 (MQ=255)

                                                                                                                  gagCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGc                                     >  2:1325717/1‑150 (MQ=255)

                                                                                                                    gCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCg                                   <  1:4762655/150‑1 (MQ=255)

                                                                                                                     cAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCgg                                  >  1:761844/1‑150 (MQ=255)

                                                                                                                      aaCGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCggg                                 >  2:145876/1‑150 (MQ=255)

                                                                                                                           ccGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGgcgc                            >  2:4801522/1‑150 (MQ=255)

                                                                                                                              ccTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGa                         <  1:4801522/150‑1 (MQ=255)

                                                                                                                                 tGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTc                      <  2:1992016/150‑1 (MQ=255)

                                                                                                                                  gcgcGGGTGAGTGCCGACGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCg                     <  2:751020/150‑1 (MQ=255)

                                                                                                                                   cgcgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCgg                    <  1:5091467/150‑1 (MQ=255)

                                                                                                                                    gcgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGt                   <  1:3502621/150‑1 (MQ=255)

                                                                                                                                    gcgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGt                   <  1:437802/150‑1 (MQ=255)

                                                                                                                                     cgGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTg                  <  1:5242830/150‑1 (MQ=255)

                                                                                                                                      gggTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGc                 <  1:2746765/150‑1 (MQ=255)

                                                                                                                                       cttcactGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCAGTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGCGTCGGTGCt                >  2:3974487/7‑150 (MQ=255)

                                                                                                                                        gTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCgg                                                                                     <  1:1702729/80‑1 (MQ=255)

                                                                                                                                           actGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGc            >  1:3777679/3‑150 (MQ=255)

                                                                                                                                           actGCCGATGCCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGc            >  1:3549067/3‑150 (MQ=255)

                                                                                                                                           aTTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGc            <  1:3398629/150‑1 (MQ=255)

                                                                                                                                            ttGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGcc           >  1:3833441/1‑150 (MQ=255)

                                                                                                                                              gCCGATGCCATCGGCAATGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTg         >  2:392317/1‑150 (MQ=255)

                                                                                                                                                cGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGa       >  2:3184178/1‑150 (MQ=255)

                                                                                                                                                    ggCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACAcc   <  1:1336096/150‑1 (MQ=255)

TCATCGTTTGCAGGACATGCTGCTCAGCCTGGCGGATAACCCGGCGCAGGCGCCGTCCTGGGCTCAATTGATGGCGCGACAGGCGCAGGTCGATCCTGACAGTTCACTGCGTCAGAGCAACGACCGCCTTGCGCGGGTGATTGCCGATGGCATCGGCAGTGAAGGCCCGGTGATCAATGTCAGCAGCGCCTGCGCGGGAGCGTCCCAGGCCATCGGCAATGCATTCCAGATGATCCGTCGCGGCGAGGTCGAGCTGGCCATTGCCGGGGGCGCCGATTCGGTGCTTAGCCTGGACACCA  >  NC_002947/3167872‑3168170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`