BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,168,314	C→A	6.1%	L261I (CTC→ATC) ‡	PP_2780 →	3‑oxoacyl‑ACP synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,168,314	0	C	A	6.1%	484.9 / 14.7	146	L261I (CTC→ATC) ‡	PP_2780	3‑oxoacyl‑ACP synthase
Reads supporting (aligned to +/- strand): ref base C (66/71); new base A (3/6); total (69/77)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 151 total aligned reads displayed.

ACACCATGACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAACA  >  NC_002947/3168165‑3168462

 cacCATGACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCt                                                                                                                                                     <  2:1317869/150‑1 (MQ=255)

       gACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCg                                                                                                                                               <  2:1650509/150‑1 (MQ=255)

         ccGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAg                                                                                                                                             <  1:781443/150‑1 (MQ=255)

         ccGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAg                                                                                                                                             <  1:1136437/150‑1 (MQ=255)

          cgcgCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAgg                                                                                                                                            >  1:3363709/1‑150 (MQ=255)

          cgcgCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAgg                                                                                                                                            <  2:3363709/150‑1 (MQ=255)

           gcgcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAggg                                                                                                                                           >  1:3805779/1‑150 (MQ=255)

           gcgcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAggg                                                                                                                                           <  1:1806363/150‑1 (MQ=255)

           gcgcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAggg                                                                                                                                           <  1:2848230/150‑1 (MQ=255)

            cgcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcga                                                                                                                                          <  2:2760348/150‑6 (MQ=255)

            cgcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcga                                                                                                                                          <  2:761844/150‑6 (MQ=255)

             gcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGc                                                                                                                                         <  1:1860869/150‑1 (MQ=255)

             gcTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCACCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGc                                                                                                                                         <  2:2158792/150‑1 (MQ=255)

              cTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGAGCGCCCgttcgagg                                                                                                                                        <  2:4445723/150‑8 (MQ=255)

               tGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGgcgc                                                                                                                                                      <  1:1478083/135‑1 (MQ=255)

               tGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGgcgc                                                                                                                                                      >  2:1478083/1‑135 (MQ=255)

               tGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCaa                                                                                                                                       >  2:2954773/1‑150 (MQ=255)

                   tCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGcc                                                                                                                                   >  1:4466877/1‑150 (MQ=255)

                   tCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGcc                                                                                                                                   >  1:4882937/1‑150 (MQ=255)

                   tCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGcc                                                                                                                                   <  2:865627/150‑1 (MQ=255)

                   tCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGcc                                                                                                                                   >  2:2656417/1‑150 (MQ=255)

                     tGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGt                                                                                                                                 >  2:728581/1‑150 (MQ=255)

                      gCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTa                                                                                                                                >  2:507560/1‑150 (MQ=255)

                       cTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTAc                                                                                                                               >  1:1362628/1‑150 (MQ=255)

                          ggCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAATGGGCGAGCGcc                                                                                                                                                 <  2:1948443/129‑1 (MQ=255)

                             gccgccAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGaa                                                                                                                         <  2:3042515/150‑1 (MQ=255)

                             gccgccAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGaa                                                                                                                         <  1:524609/150‑1 (MQ=255)

                             gccgccAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGaa                                                                                                                         <  1:1907093/150‑1 (MQ=255)

                                gccAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTg                                                                                                                      >  1:2526288/1‑150 (MQ=255)

                                   agcagcGACCAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTg                                                                                                                   <  1:3974487/150‑1 (MQ=255)

                                   agcagcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTg                                                                                                                   >  1:2451644/1‑150 (MQ=255)

                                    gcagcGAACCGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTgg                                                                                                                  <  1:163937/150‑1 (MQ=255)

                                    gcagcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTgg                                                                                                                  <  2:2471039/150‑1 (MQ=255)

                                    gcagcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTgg                                                                                                                  <  1:3754994/150‑1 (MQ=255)

                                    gcagcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTgg                                                                                                                  <  2:555341/150‑1 (MQ=255)

                                       gcGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGtt                                                                                                               <  2:1837367/150‑1 (MQ=255)

                                        cGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGttt                                                                                                              >  1:2366048/1‑150 (MQ=255)

                                         gAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTc                                                                                                             >  2:425745/1‑150 (MQ=255)

                                          aaCAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCg                                                                                                            <  2:4867161/150‑1 (MQ=255)

                                                gTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGgcagca                                                                                                      >  2:4875768/1‑150 (MQ=255)

                                                gTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGgcagca                                                                                                      >  2:2140724/1‑150 (MQ=255)

                                                  tGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCagcagc                                                                                                    <  1:1366000/150‑1 (MQ=255)

                                                        aCCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCCACGCCGTACGCCGAAGTGCTGGGTGTCGGCAGCAGCCTGGAt                                                                                              >  1:2799863/1‑150 (MQ=255)

                                                        aCCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGAt                                                                                              <  1:3729575/150‑1 (MQ=255)

                                                          cGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGc                                                                                            >  2:318064/1‑150 (MQ=255)

                                                          cGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGc                                                                                            >  2:4706940/1‑150 (MQ=255)

                                                          cGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGc                                                                                            <  1:425745/150‑1 (MQ=255)

                                                                gtgtCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACaa                                                                                      <  2:4594299/150‑1 (MQ=255)

                                                                 tgtCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGTGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAGCGGGCGCTCGCCCGGGGGGCAACGCCGTACGCCGGAGTGCTGGGTGTCGGCCGCGGCGTGGAGGccggccgg                                                                                     >  2:2996841/1‑144 (MQ=255)

                                                                   tCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAAt                                                                                   <  1:2277090/150‑1 (MQ=255)

                                                                     ggCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCa                                                                                 >  2:2696188/1‑150 (MQ=255)

                                                                      gCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCAc                                                                                >  2:3103504/1‑150 (MQ=255)

                                                                      gCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCAc                                                                                <  2:3896964/150‑1 (MQ=255)

                                                                       ccGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCAcc                                                                               <  1:2526242/150‑1 (MQ=255)

                                                                        cGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCg                                                                              <  2:153154/150‑1 (MQ=255)

                                                                         gTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGc                                                                             <  1:1708438/150‑1 (MQ=255)

                                                                               tCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCacccga                                                                       >  1:2305495/1‑150 (MQ=255)

                                                                                 gCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCacccgacc                                                                     >  1:1365306/1‑150 (MQ=255)

                                                                                    aTCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGc                                                                  <  2:432069/150‑1 (MQ=255)

                                                                                       gCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAgg                                                               <  2:1569281/150‑1 (MQ=255)

                                                                                        cAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAggg                                                              >  1:2602146/1‑150 (MQ=255)

                                                                                        cAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGAACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAggg                                                              >  2:1268444/1‑150 (MQ=255)

                                                                                             gCTTGTTGGCGGGTGAGGGCGGTGGTTTTGTCGGGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAACTCACCGCACCCGACCCGCAGGGCCggg                                                         <  1:4680987/150‑1 (MQ=255)

                                                                                             gCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCggg                                                         <  1:1605585/150‑1 (MQ=255)

                                                                                             gCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCggg                                                         <  1:3496618/150‑1 (MQ=255)

                                                                                             gCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCggg                                                         <  2:2586987/150‑1 (MQ=255)

                                                                                               ttGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGgc                                                       >  1:1045438/1‑150 (MQ=255)

                                                                                                 gATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGgcgc                                                     <  1:653768/150‑1 (MQ=255)

                                                                                                   gggCGGGGGAGGGCGGTGGCTTGGGCGGGCGCGACCCCCGCGACCGGGCGCGCGCCCGAGGGGCCCCGCCGTACGCCGCAGTGCTGGGGTGCGGCAGCAGCCTGGATGCCTACCCACTCACCGCACCCGACCCGCAGGGCCGGGGCGCgg                                                   <  2:4675878/148‑1 (MQ=255)

                                                                                                       gggTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCt                                               >  2:631081/1‑150 (MQ=255)

                                                                                                       gggTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCt                                               >  2:2466540/1‑150 (MQ=255)

                                                                                                         gTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTgg                                             <  2:3978640/150‑1 (MQ=255)

                                                                                                         gTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTgg                                             >  1:832875/1‑150 (MQ=255)

                                                                                                         gTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTgg                                             <  2:3535552/150‑1 (MQ=255)

                                                                                                               gCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGc                                       <  2:209239/150‑1 (MQ=255)

                                                                                                                  gtggtTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAgg                                    <  1:318064/150‑1 (MQ=255)

                                                                                                                   tggtTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAggc                                   >  2:1469795/1‑150 (MQ=255)

                                                                                                                   tgattttGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCa                                                                                 <  1:1284537/101‑1 (MQ=255)

                                                                                                                     gtTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAggcgg                                 >  2:1325124/1‑150 (MQ=255)

                                                                                                                      ttttGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAggcggc                                <  1:3480236/150‑1 (MQ=255)

                                                                                                                       tttGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGcc                               <  2:1860107/150‑1 (MQ=255)

                                                                                                                       tttGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGcc                               >  2:4938811/1‑150 (MQ=255)

                                                                                                                       tttGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGcc                               >  1:2610946/1‑150 (MQ=255)

                                                                                                                          gTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTg                            >  1:3657048/1‑150 (MQ=255)

                                                                                                                           tCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGa                           <  1:1037456/150‑1 (MQ=255)

                                                                                                                             gTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACg                         <  1:2954773/150‑1 (MQ=255)

                                                                                                                             gTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACg                         <  1:1561267/150‑1 (MQ=255)

                                                                                                                               gCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGa                       <  1:3022545/150‑1 (MQ=255)

                                                                                                                                     aaCCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCgg                 <  1:2404101/150‑1 (MQ=255)

                                                                                                                                       cccTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGc               <  2:1742678/150‑1 (MQ=255)

                                                                                                                                        ccTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCt              <  2:3805779/150‑1 (MQ=255)

                                                                                                                                         cTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTg             >  2:5210676/1‑150 (MQ=255)

                                                                                                                                          tCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTgg            >  1:4481025/1‑150 (MQ=255)

                                                                                                                                          tCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTgg            >  1:4669159/1‑150 (MQ=255)

                                                                                                                                          cccctcGGGCGAGCGCCCGTGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTgg            >  1:2481602/6‑150 (MQ=255)

                                                                                                                                              aCGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAACATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGcgcc        <  2:940247/150‑1 (MQ=255)

                                                                                                                                              aCGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGcgcc        <  2:893824/150‑1 (MQ=255)

                                                                                                                                                 ggCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCa     >  1:1114282/1‑150 (MQ=255)

                                                                                                                                                   cgcTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAAc   <  2:629350/150‑1 (MQ=255)

                                                                                                                                                    gcTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAACa  <  1:4903579/150‑1 (MQ=255)

ACACCATGACCGCGCTGTATCTGCTCGGCGCCGCCAGCAGCGAACAGCGTTGGGGCACCGAACTGTGTCGGCCGTTCGATCGCCATCGCAGTGGCTTGATTGCGGGTGAGGGCGGTGGTTTTGTCGTGCTCGAAACCCTCGAACGGGCGCTCGCCCGAGGGGCAACGCCGTACGCCGAAGTGCTGGGTTTCGGCAGCAGCCTGGATGCCTACAAAATCACCGCACCCGACCCGCAGGGCCGGGGCGCGGTGCTGGCGATGCAGGCGGCCCTGACGGATGCCGGGCTGGCGCCGCAACA  >  NC_002947/3168165‑3168462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`