BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,262,321	T→A	8.5%	Q155L (CAG→CTG)	PP_2857 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,262,321	0	T	A	8.5%	384.7 / 17.1	122	Q155L (CAG→CTG)	PP_2857	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (62/49); new base A (4/7); total (66/56)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 124 total aligned reads displayed.

GCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGCACCTGCGCTC  >  NC_002947/3262172‑3262470

gCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCt                                                                                                                                                       <  2:29713/150‑1 (MQ=255)

 cTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTg                                                                                                                                                      <  1:1572964/150‑1 (MQ=255)

  tATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCCGCTACCGCTGAAGGCCTGGACACTGGTAGTGACCTgg                                                                                                                                                     >  2:3034387/1‑150 (MQ=255)

   aTTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGACCAGGc                                                                                                                                                    <  2:4786049/150‑1 (MQ=255)

   aTTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGACCAGGc                                                                                                                                                    <  1:4151946/150‑1 (MQ=255)

   aTTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCATCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGACCAGGc                                                                                                                                                    <  2:1309616/150‑1 (MQ=255)

    ttAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCAGGTCACTACCAGTGACCAGGcc                                                                                                                                                   <  1:54830/150‑2 (MQ=255)

         ttAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGc                                                                                                                                              >  2:222196/1‑150 (MQ=255)

         ttAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTAACGGTGAAGGCCAGGTCACTGGTAGTGACCTTGCGATAGc                                                                                                                                              >  2:222116/1‑150 (MQ=255)

         ttAAGTGTATTTCTTAACTGCAGGGTCAATCCGCGAGACGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCACTCGGCTACCGGTGAAGGCCTGGTCACTTGTAGTTACCTGGCGATAGc                                                                                                                                              <  2:5388053/150‑1 (MQ=255)

            aGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCctg                                                                                                                                           <  2:2368438/150‑1 (MQ=255)

                  gTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGaa                                                                                                                                     >  1:5098419/1‑150 (MQ=255)

                   ttCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGTCTACCGGTGAAGGCCTGGTCACTACCAGTGACCAGGcc                                                                                                                                                   <  2:2817821/135‑2 (MQ=255)

                   ttCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTACCAGTGACCAGGcc                                                                                                                                                   >  1:2817821/1‑134 (MQ=255)

                         aCTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGt                                                                                                                              <  2:2593616/150‑1 (MQ=255)

                           tGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTg                                                                                                                            >  1:4939309/1‑150 (MQ=255)

                               ttgagcaTCCGGGTCGCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTcc                                                                                                                        >  1:4140459/7‑150 (MQ=255)

                                    aaTCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGa                                                                                                                     >  2:556717/1‑148 (MQ=255)

                                    aaTCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGa                                                                                                                     <  1:556717/148‑1 (MQ=255)

                                    aaTCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgt                                                                                                                   >  2:3503235/1‑150 (MQ=255)

                                    aaTCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgt                                                                                                                   >  1:1909227/1‑150 (MQ=255)

                                      tacgcgACCATGATGATCAAGCTGATGTCCATTCGGTGTCCAACCACGGCACTATGGTTCAACTGGATCACCACTCGGCTACAGGAGTAGGCAAGGTCACTGGTAGTGACAAGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgatg                                                                                                                 >  2:3611224/3‑150 (MQ=255)

                                      tCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGACTGTCAGTTGCTCCTGAgttg                                                                                                                 >  2:3611225/1‑150 (MQ=255)

                                      tCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgttg                                                                                                                 >  2:3611222/1‑150 (MQ=255)

                                      tCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTAGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgttg                                                                                                                 >  2:4725031/1‑150 (MQ=255)

                                        cgcgACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgttgtt                                                                                                               <  2:722628/150‑1 (MQ=255)

                                        cgcgACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgttgtt                                                                                                               <  2:2511395/150‑1 (MQ=255)

                                         gcgACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGttgttg                                                                                                              >  2:2768352/1‑150 (MQ=255)

                                         gcgACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGttgttg                                                                                                              <  2:28784/150‑1 (MQ=255)

                                             cccGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCa                                                                                                          <  1:5039761/150‑1 (MQ=255)

                                               cGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAgc                                                                                                        <  1:2883219/150‑1 (MQ=255)

                                                    gCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCAc                                                                                                   <  1:4707019/150‑1 (MQ=255)

                                                      tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGt                                                                                                 >  1:1253123/1‑150 (MQ=255)

                                                      tCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGt                                                                                                 >  1:1253496/1‑150 (MQ=255)

                                                        aaGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTc                                                                                               >  1:3713758/1‑150 (MQ=255)

                                                          ggTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTg                                                                                             <  2:4553066/150‑1 (MQ=255)

                                                           gtgtTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGt                                                                                            >  1:4223107/1‑150 (MQ=255)

                                                           gtgtTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGt                                                                                            >  2:4934761/1‑150 (MQ=255)

                                                            tgtTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGtt                                                                                           <  1:3878904/150‑1 (MQ=255)

                                                             gtTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTg                                                                                          <  2:181268/150‑1 (MQ=255)

                                                              ttGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGc                                                                                         >  2:1518317/1‑150 (MQ=255)

                                                               tGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCa                                                                                        <  2:1516733/150‑1 (MQ=255)

                                                                  ccATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGa                                                                                     <  2:2286356/150‑1 (MQ=255)

                                                                   cATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAg                                                                                    <  2:1056342/150‑1 (MQ=255)

                                                                    aTTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAgttgt                                                                                                                <  1:633069/121‑1 (MQ=255)

                                                                    aTTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAgg                                                                                   >  2:1608386/1‑150 (MQ=255)

                                                                    aTTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAgg                                                                                   >  2:4802980/1‑150 (MQ=255)

                                                                     ttCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAAt                                                                                                                                    >  1:1902833/1‑100 (MQ=255)

                                                                     ttCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAAt                                                                                                                                    <  2:1902833/100‑1 (MQ=255)

                                                                       cGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACg                                                                                <  2:4950050/150‑1 (MQ=255)

                                                                              ccACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtcgt                                                                         >  2:350400/1‑150 (MQ=255)

                                                                               cACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTgtcgtc                                                                        >  2:127484/1‑150 (MQ=255)

                                                                                aCCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGtcgtcg                                                                       >  2:3017451/1‑150 (MQ=255)

                                                                                aCCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGtcgtcg                                                                       >  2:144453/1‑150 (MQ=255)

                                                                                aCCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTGCTGTTGCATGAGGACGCTGtcgtcg                                                                       >  2:1894030/1‑150 (MQ=255)

                                                                                 ccccGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGa                                                                      <  1:4718848/150‑1 (MQ=255)

                                                                                         ctctGGTTCAACTGGATCACCCCTCGGCTCCCGGTGAAGGCCTGGTCACTGGTCGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTGCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGc                                                              >  2:3751404/1‑150 (MQ=255)

                                                                                          tctGGTTCAACTGTATCACCCCTCGGCTACCGGTGAAGGCCTGGTCTCTGTTAGTGACCTGGCGATATCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCttttct                                                             <  1:3194164/150‑4 (MQ=255)

                                                                                          tctGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCa                                                                                                                                <  1:4000077/83‑1 (MQ=255)

                                                                                          tctGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCa                                                                                                                                >  2:4000077/1‑83 (MQ=255)

                                                                                          tctGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCg                                                             <  2:1936425/150‑1 (MQ=255)

                                                                                          tctGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCg                                                             <  2:4654288/150‑1 (MQ=255)

                                                                                          tctGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCg                                                             >  1:3363343/1‑150 (MQ=255)

                                                                                          tctGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCg                                                             >  1:4732256/1‑150 (MQ=255)

                                                                                              gTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTtg                                                         <  2:2126308/150‑1 (MQ=255)

                                                                                               ttCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTtgt                                                        <  1:581931/150‑1 (MQ=255)

                                                                                                  aaCTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCa                                                     >  1:1055583/1‑150 (MQ=255)

                                                                                                       gttCAACCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGACTGCTGGAATGTCAGTTGCTGCTGAGTTGTTGATCAGCGCCACGTTGTGTTGCATGTGGTCGCTGTCGTCGATGCTTTGGGGTTGTGCACCAAt                                                >  2:4649028/3‑150 (MQ=255)

                                                                                                         tCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATc                                                                                                           <  1:514199/89‑1 (MQ=255)

                                                                                                         tCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATc                                                                                                           >  2:514199/1‑89 (MQ=255)

                                                                                                         tCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGc                                              <  1:2121371/150‑1 (MQ=255)

                                                                                                             cccTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGAt                                          >  1:2959325/1‑150 (MQ=255)

                                                                                                                  ggCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCa                                     <  2:3594039/150‑1 (MQ=255)

                                                                                                                  ggCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCa                                     <  1:1456846/150‑1 (MQ=255)

                                                                                                                   gCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAg                                    >  2:2569473/1‑150 (MQ=255)

                                                                                                                      aCCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGc                                 <  2:705823/150‑1 (MQ=255)

                                                                                                                        cGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGt                               >  1:2917450/1‑150 (MQ=255)

                                                                                                                        cGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGt                               >  1:1765456/1‑150 (MQ=255)

                                                                                                                        cGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGt                               >  2:600748/1‑150 (MQ=255)

                                                                                                                          cataaGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTAGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTg                             >  2:3663189/4‑150 (MQ=255)

                                                                                                                           tGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTgg                            <  2:2917450/150‑1 (MQ=255)

                                                                                                                            gAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGc                           <  1:4421674/150‑1 (MQ=255)

                                                                                                                                 ccaggTCACTACCAGTGACCAGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGa                                                                                     <  1:3265555/84‑1 (MQ=38)

                                                                                                                                 ccaggTCACTACCAGTGACCAGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGa                                                                                     >  2:3265555/4‑87 (MQ=38)

                                                                                                                                  caggTCACTACCAGTGACCAGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAg                                                                                    >  1:3268983/3‑87 (MQ=38)

                                                                                                                                  caggTCACTACCAGTGACCAGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAg                                                                                    <  2:3268983/85‑1 (MQ=38)

                                                                                                                                  cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCCGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTg                     >  2:1700209/1‑150 (MQ=255)

                                                                                                                                  cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTg                     >  1:4025432/1‑150 (MQ=255)

                                                                                                                                  cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTg                     >  2:4317458/1‑150 (MQ=255)

                                                                                                                                  cTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTg                     >  2:2883391/1‑150 (MQ=255)

                                                                                                                                   tGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTgg                    <  1:4649028/150‑1 (MQ=255)

                                                                                                                                        aCTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGtt               >  1:1890991/1‑150 (MQ=255)

                                                                                                                                         cTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTg              <  1:2245631/150‑1 (MQ=255)

                                                                                                                                          tGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTgg             >  2:4040883/1‑150 (MQ=255)

                                                                                                                                          tGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTgg             >  2:722427/1‑150 (MQ=255)

                                                                                                                                           ggTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGc            <  1:222116/150‑1 (MQ=255)

                                                                                                                                           ggTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGc            <  1:222196/150‑1 (MQ=255)

                                                                                                                                            gTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGCa           >  2:659122/1‑150 (MQ=255)

                                                                                                                                                    cTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGCACCTGCGct   >  2:1883351/1‑150 (MQ=255)

                                                                                                                                                     tGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGCACCTGCGctc  >  1:3919055/1‑150 (MQ=255)

GCTATTAGGTTAAGTGTCGTTCTTAACTGCAGGGTCAATCCGCGACCCGGATGCTCAAGGTGTTGGCCATTCGGTTTCCCACCCCGGCACTCTGGTTCAACTGGATCACCCCTCGGCTACCGGTGAAGGCCTGGTCACTGGTAGTGACCTGGCGATAGCCTGCTGGAATGTCAGTTGCTCCTGAGTTGTTGATCAGCGCCACGTTCTGTTGCATGAGGACGCTGTCGTCGATGCTTTGCGGTTGTGCACCAATGCTGATGCTCAGTGCGTTGGCTTGCTGGGTGTTGGCACCTGCGCTC  >  NC_002947/3262172‑3262470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`