BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	226,226	T→A	6.2%	L151Q (CTG→CAG)	PP_0174 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	226,226	0	T	A	6.2%	527.9 / 17.8	161	L151Q (CTG→CAG)	PP_0174	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (77/74); new base A (2/8); total (79/82)
Fisher's exact test for biased strand distribution p-value = 9.90e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

Only 100 of 174 total aligned reads displayed.

GGCCGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTACC  >  NC_002947/226077‑226375

ggCCGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCAtgct                                                                                                                                                       <  2:5154929/150‑1 (MQ=255)

   cGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCa                                                                                                                                                    >  2:1642571/1‑150 (MQ=255)

           tGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTAGGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcgg                                                                                                                                            <  2:4354761/150‑3 (MQ=255)

            gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgccgc                                                                                                                                           >  1:141505/1‑150 (MQ=255)

            gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgccgc                                                                                                                                           <  1:602354/150‑1 (MQ=255)

            gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgccgc                                                                                                                                           <  1:2843738/150‑1 (MQ=255)

            gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATgccgccgc                                                                                                                                           <  1:2775940/150‑1 (MQ=255)

            gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcggt                                                                                                                                           <  2:1563870/150‑4 (MQ=255)

            gTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCAGCATgccgcggt                                                                                                                                           <  1:2693461/150‑4 (MQ=255)

            gTTGCGCAGGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACTGCGGCATGCAGCATgccgcggt                                                                                                                                           <  2:2130531/150‑4 (MQ=255)

                cgcATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACGGCGGCATGCAGCATgccgcggtcgaa                                                                                                                                       <  1:210579/150‑8 (MQ=255)

                cgcATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACGGCGGCATGCAGCATgccgcggtcgaa                                                                                                                                       <  1:2648038/150‑8 (MQ=255)

                      gcggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGt                                                                                                                                 <  2:5241663/150‑1 (MQ=255)

                        ggcCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGa                                                                                                                               <  1:1120656/150‑1 (MQ=255)

                         gcCCTGCAGGGCAATGCTTCTGCCTTTTTCAACCTTGGCCAGCAGCACTAGTTCGGCAAAGGCATCTACCCTTACTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGTCCGCGGCATGCTGCATGGCGCCTCCGCACTGGGttag                                                                                                                              <  2:5016207/150‑4 (MQ=255)

                           ccTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCt                                                                                                                            >  2:4970435/1‑150 (MQ=255)

                           ccTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCt                                                                                                                            >  2:3223596/1‑150 (MQ=255)

                             tGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGt                                                                                                                          >  1:4759590/1‑150 (MQ=255)

                             tGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGt                                                                                                                          >  1:670536/1‑150 (MQ=255)

                              gCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTa                                                                                                                         >  2:2351004/1‑150 (MQ=255)

                               cAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTAt                                                                                                                        <  2:5322386/150‑1 (MQ=255)

                                 gggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATga                                                                                                                      <  1:3675241/150‑1 (MQ=255)

                                  ggCAATGCTGCTTCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCACAGGCATCGACCCTTCCTATGTGCCGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                     <  1:3942240/150‑1 (MQ=255)

                                  ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTCTgag                                                                                                                     >  1:5013662/1‑150 (MQ=255)

                                  ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                     >  1:718797/1‑150 (MQ=255)

                                  ggCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgag                                                                                                                     >  1:4819872/1‑150 (MQ=255)

                                     aaTGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGTGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggag                                                                                                                  <  1:3516853/150‑1 (MQ=255)

                                     aaTGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggag                                                                                                                  <  1:584316/150‑1 (MQ=255)

                                       tgctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggagga                                                                                                                >  2:1264075/1‑150 (MQ=255)

                                       tgctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggagga                                                                                                                <  1:3268784/150‑1 (MQ=255)

                                        gctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggaggag                                                                                                               <  1:1678605/150‑1 (MQ=255)

                                        gctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggaggag                                                                                                               <  1:771015/150‑1 (MQ=255)

                                        gctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggaggag                                                                                                               <  2:4231720/150‑1 (MQ=255)

                                        gctgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTCCGGCAACGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATgaggaggag                                                                                                               <  2:2281458/150‑1 (MQ=255)

                                          tgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGgt                                                                                                             >  2:3436819/1‑150 (MQ=255)

                                          tgctgcCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACGGGGTGACCTGTATGAGGAGGAGgt                                                                                                             >  2:701489/1‑150 (MQ=255)

                                                cTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGa                                                                                                       >  1:3740164/1‑150 (MQ=255)

                                                  ggTTCAACCTGGGCCAGCAGCACTACTGCGGCAAAGGCAGCGACCCTTCCTAGGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTGCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACg                                                                                                     <  2:543242/150‑1 (MQ=255)

                                                   gTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACgg                                                                                                    >  1:4279311/1‑150 (MQ=255)

                                                   gTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACgg                                                                                                    >  1:1533552/1‑150 (MQ=255)

                                                    ttCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGt                                                                                                   >  2:694330/1‑150 (MQ=255)

                                                    ttCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGt                                                                                                   >  1:3853795/1‑150 (MQ=255)

                                                       aaCCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGAc                                                                                                >  2:3718367/1‑150 (MQ=255)

                                                       aaCCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGAc                                                                                                <  1:1037195/150‑1 (MQ=255)

                                                         ccTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCt                                                                                              <  1:3449609/150‑1 (MQ=255)

                                                          cTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTg                                                                                             >  2:3843365/1‑150 (MQ=255)

                                                          cTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTAGGAGGAGGAGGTGTGCGACGGTGACCTg                                                                                             >  1:248022/1‑150 (MQ=255)

                                                               ccAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGGATGAGGAGGAGGTGTGCGACGGTGACCTGGAATg                                                                                        >  2:1437898/1‑150 (MQ=255)

                                                                      caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGtt                                                                                 >  1:3354899/1‑150 (MQ=255)

                                                                      caCTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGtt                                                                                 >  1:3858064/1‑150 (MQ=255)

                                                                       actactTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTg                                                                                <  2:262114/150‑1 (MQ=255)

                                                                           ctTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCt                                                                            >  2:2025599/1‑150 (MQ=255)

                                                                            ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                           >  2:1879314/1‑150 (MQ=255)

                                                                            ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                           >  1:2093018/1‑150 (MQ=255)

                                                                            ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTg                                                                           >  2:1879315/1‑150 (MQ=255)

                                                                            ttCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAAAGGCAGGTTGACCTg                                                                           >  2:2248020/1‑150 (MQ=255)

                                                                              cGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGTAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGt                                                                         <  1:544847/150‑1 (MQ=255)

                                                                                  aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCaa                                                                     <  1:867170/150‑1 (MQ=255)

                                                                                  aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCaa                                                                     >  1:4553601/1‑150 (MQ=255)

                                                                                  aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCaa                                                                     >  2:1317231/1‑150 (MQ=255)

                                                                                  aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCaa                                                                     >  1:3018909/1‑150 (MQ=255)

                                                                                  aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTACGACCGCGGCAtgctg                                                                                                                                                      >  2:2566007/1‑69 (MQ=255)

                                                                                  aaaGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTACGACCGCGGCAtgctg                                                                                                                                                      <  1:2566007/69‑1 (MQ=255)

                                                                                    aGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGc                                                                   <  2:478687/150‑1 (MQ=255)

                                                                                    aGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAAGGGCAGGTTGACCTGGTGCAAGc                                                                   <  2:3031103/150‑1 (MQ=255)

                                                                                        aTCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTAt                                                               >  2:4876286/1‑150 (MQ=255)

                                                                                        aTCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTAt                                                               <  2:3853795/150‑1 (MQ=255)

                                                                                             cccTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGCCCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTg                                                          <  2:3592049/150‑1 (MQ=255)

                                                                                             cccTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTg                                                          <  2:1124752/150‑1 (MQ=255)

                                                                                             cccTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTg                                                          <  2:3903854/150‑1 (MQ=255)

                                                                                             cccTTCCTAGGTGCAGGCGGCGGAGTGTGCCCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTg                                                          <  2:3162439/150‑1 (MQ=255)

                                                                                                    tATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTg                                                   >  1:2279900/1‑150 (MQ=255)

                                                                                                       gtgCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                                <  2:172702/150‑1 (MQ=255)

                                                                                                        tgCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTg                                               >  1:2011276/1‑150 (MQ=255)

                                                                                                             gcggcgGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGcc                                          >  2:3266426/1‑150 (MQ=255)

                                                                                                                     gtgtTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAggg                                  >  1:1859550/1‑150 (MQ=255)

                                                                                                                         tACCGGCTGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAg                              <  1:2872401/150‑1 (MQ=255)

                                                                                                                         tACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGAc                                                                                                <  1:2131112/84‑1 (MQ=255)

                                                                                                                              gCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGgcgc                          >  1:5163649/1‑149 (MQ=255)

                                                                                                                               cAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTgg                                                         >  2:3361087/1‑117 (MQ=255)

                                                                                                                                aGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGtt                       >  1:3242400/1‑150 (MQ=255)

                                                                                                                                aGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGtt                       <  2:2484794/150‑1 (MQ=255)

                                                                                                                                aGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGtt                       >  2:4403210/1‑150 (MQ=255)

                                                                                                                                aGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGtt                       <  2:78842/150‑1 (MQ=255)

                                                                                                                                  gCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGcgtcgt                     >  1:5062256/1‑150 (MQ=255)

                                                                                                                                   ccTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                    >  1:5119617/1‑150 (MQ=255)

                                                                                                                                   ccTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                    >  1:1871980/1‑150 (MQ=255)

                                                                                                                                   ccTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTc                    <  2:141505/150‑1 (MQ=255)

                                                                                                                                       cGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGttt                <  2:1574562/150‑1 (MQ=255)

                                                                                                                                       cGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCAAGGCGCGTTGTCGttt                <  1:1143598/150‑1 (MQ=255)

                                                                                                                                         gcggcgGCATGCAGCATGCCGCGGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTg              >  2:5987/4‑150 (MQ=255)

                                                                                                                                         acagcgGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTg              >  2:2223301/4‑150 (MQ=255)

                                                                                                                                             cgGCATGCAGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAAt          >  1:3816556/1‑150 (MQ=255)

                                                                                                                                              ggCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATg         <  2:114904/150‑1 (MQ=255)

                                                                                                                                                   gctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                                <  2:463211/106‑1 (MQ=255)

                                                                                                                                                   gctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGt                                                >  1:463211/1‑106 (MQ=255)

                                                                                                                                                    ctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCa                                                                                     >  1:858186/1‑68 (MQ=255)

                                                                                                                                                    ctgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCa                                                                                     <  2:858186/68‑1 (MQ=255)

                                                                                                                                                     tgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTAcc  >  1:722304/1‑150 (MQ=255)

                                                                                                                                                     tgcATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTGGTCGTTTTGAAATGGGCTAcc  >  1:1576424/1‑150 (MQ=255)

GGCCGGGCACCTGTTGCGCATGGCGGCCCTGCAGGGCAATGCTGCTGCCTGGTTCAACCTTGGCCAGCAGCACTACTTCGGCAAAGGCATCGACCCTTCCTATGTGCAGGCGGCGGAGTGTTACCGGCAGGCCTTCGACCGCGGCATGCTGCATGCCGCCGCCGCACTGGGTGACCTGTATGAGGAGGAGGTGTGCGACGGTGACCTGGAATGGCAGGTTGACCTGGTGCAAGCCTATCAGTGGTTCTTGCGTGGCGCCGAACAGGGCGAGGCGCGTTGTCGTTTTGAAATGGGCTACC  >  NC_002947/226077‑226375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`