BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,319,178	C→G	5.0%	P608P (CCG→CCC)	treSA ←	trehalose synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,319,178	0	C	G	5.0%	398.1 / 8.6	119	P608P (CCG→CCC)	treSA	trehalose synthase A
Reads supporting (aligned to +/- strand): ref base C (53/60); new base G (4/2); total (57/62)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 125 total aligned reads displayed.

CGACCGTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCACGC  >  NC_002947/3319030‑3319325

cGACCGTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCg                                                                                                                                                    <  2:4419125/150‑1 (MQ=255)

 gACCGTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCgg                                                                                                                                                   <  2:1530205/150‑1 (MQ=255)

    cGTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGgct                                                                                                                                                <  1:5440362/150‑1 (MQ=255)

      tctcGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGgctgc                                                                                                                                              >  2:2299407/1‑150 (MQ=255)

        tcGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTc                                                                                                                                            <  2:2382784/150‑1 (MQ=255)

          gTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCGGGgctgctggt                                                                                                                                          <  1:5099509/150‑4 (MQ=255)

          gTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCAc                                                                                                                                          >  1:1065805/1‑150 (MQ=255)

          gTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCAc                                                                                                                                          <  2:3798001/150‑1 (MQ=255)

              aTCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCa                                                                                                                                      <  2:3566200/150‑1 (MQ=255)

               tCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAg                                                                                                                                     <  1:3830236/150‑1 (MQ=255)

                cATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCAc                                                                                                                                          >  1:4111852/1‑144 (MQ=255)

                 aTGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCCGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGt                                                                                                                                   >  2:548745/1‑150 (MQ=255)

                   gTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTAc                                                                                                                                 <  1:5398112/150‑1 (MQ=255)

                     cGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCa                                                                                                                               <  1:959598/150‑1 (MQ=255)

                        ccaccGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCCATCGCGTCCTGGCCGAAGTTCAGCGCCGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCa                                                                                                                            >  2:567834/1‑150 (MQ=255)

                         caccGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCAc                                                                                                                           <  1:589603/150‑1 (MQ=255)

                          accGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCAcc                                                                                                                          <  1:3289007/150‑1 (MQ=255)

                          accGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCAcc                                                                                                                          <  1:3289053/150‑1 (MQ=255)

                          accGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCAcc                                                                                                                          <  1:3518529/150‑1 (MQ=255)

                           ccGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCt                                                                                                                         >  2:3639859/1‑150 (MQ=255)

                              gCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGAc                                                                                                                      <  2:2969117/150‑1 (MQ=255)

                                ccAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTGCTGGTACCTGaggt                                                                                                                    <  2:522919/150‑4 (MQ=255)

                                 cAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGc                                                                                                                   <  2:1143938/150‑1 (MQ=255)

                                 cAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGc                                                                                                                   >  1:3636639/1‑150 (MQ=255)

                                       tgAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACGAGCACCTGACGGCtggtgg                                                                                                             <  1:368360/150‑1 (MQ=255)

                                        gAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGc                                                                                                            <  1:2608972/150‑1 (MQ=255)

                                        gAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGc                                                                                                            >  1:2505640/1‑150 (MQ=255)

                                           cccGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATCCCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGAt                                                                                                         >  1:3813568/1‑150 (MQ=255)

                                           cccGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGAt                                                                                                         <  2:4152128/150‑1 (MQ=255)

                                           cccGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGAt                                                                                                         >  1:5068617/1‑150 (MQ=255)

                                           cccGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGAt                                                                                                         >  1:5068393/1‑150 (MQ=255)

                                                 cAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCCGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTa                                                                                                   <  1:3448088/150‑1 (MQ=255)

                                                 cAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTa                                                                                                   >  1:1275419/1‑150 (MQ=255)

                                                    cagcagTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGc                                                                                                       <  2:3872707/143‑1 (MQ=255)

                                                    cagcagTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGc                                                                                                <  1:2160424/150‑1 (MQ=255)

                                                         gTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGgc                                                                                           <  1:5284335/150‑1 (MQ=255)

                                                          ttCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGGTCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGgctgc                                                                                                                                              <  1:3364604/98‑1 (MQ=255)

                                                          ttCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGGTCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGgctgc                                                                                                                                              >  2:3364604/1‑98 (MQ=255)

                                                            ccTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGca                                                                                        >  1:2827583/1‑150 (MQ=255)

                                                             cTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcac                                                                                       >  2:930562/1‑150 (MQ=255)

                                                             cTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcac                                                                                       >  1:2560633/1‑150 (MQ=255)

                                                             cTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcac                                                                                       >  2:930715/1‑150 (MQ=255)

                                                              tCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGcaca                                                                                      >  1:5196476/1‑150 (MQ=255)

                                                                ggCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCAcagc                                                                                    >  1:523551/1‑150 (MQ=255)

                                                                ggCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCAcagc                                                                                    >  1:570615/1‑150 (MQ=255)

                                                                          tCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTttc                                                                          >  2:2950071/1‑150 (MQ=255)

                                                                          tCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTttc                                                                          <  1:3144093/150‑1 (MQ=255)

                                                                                ccGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCAcc                                                                    >  1:5277119/1‑150 (MQ=255)

                                                                                ccGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCACGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCAcc                                                                    <  2:3392238/150‑1 (MQ=255)

                                                                                 cGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCt                                                                   >  1:447573/1‑150 (MQ=255)

                                                                                     gTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCa                                                               <  2:739135/150‑1 (MQ=255)

                                                                                     gTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCa                                                               <  2:4836899/150‑1 (MQ=255)

                                                                                     gTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCa                                                               <  2:2951877/150‑1 (MQ=255)

                                                                                      ttCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAg                                                              >  1:2269973/1‑150 (MQ=255)

                                                                                       tCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTc                                                                                                                                            <  2:513176/71‑1 (MQ=255)

                                                                                       tCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTc                                                                                                                                            >  1:513176/1‑71 (MQ=255)

                                                                                            gcAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAAt                                                        <  1:1162072/150‑1 (MQ=255)

                                                                                                tGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAAt                                                    <  1:1580137/150‑1 (MQ=255)

                                                                                                tGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAAt                                                    >  1:988753/1‑150 (MQ=255)

                                                                                                  aTCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCa                                                  >  1:4045711/1‑150 (MQ=255)

                                                                                                  aTCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCa                                                  >  2:924833/1‑150 (MQ=255)

                                                                                                  aTCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCa                                                  <  2:5348011/150‑1 (MQ=255)

                                                                                                     tGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCActc                                               >  1:4063368/1‑150 (MQ=255)

                                                                                                     tGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCActc                                               >  2:4554339/1‑150 (MQ=255)

                                                                                                       gATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACtctc                                             <  1:433131/150‑1 (MQ=255)

                                                                                                       gATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACtctc                                             <  1:1051655/150‑1 (MQ=255)

                                                                                                         tACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGt                                           >  2:1949882/1‑150 (MQ=255)

                                                                                                          aCCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTc                                          >  2:389504/1‑150 (MQ=255)

                                                                                                            cgcgCCCGGCTGGCAGCTCATGCGCCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAATTGAATCACTCTCGTcga                                        >  1:2940280/1‑148 (MQ=255)

                                                                                                            cgcgCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCGCCTTGCAGGCAAATGAATCACTCTCGTCCa                                        >  2:3238611/1‑150 (MQ=255)

                                                                                                               gcCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCt                                     >  1:3388056/1‑150 (MQ=255)

                                                                                                               gcCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCt                                     <  1:1310714/150‑1 (MQ=255)

                                                                                                                 ccGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCGCGTCCAGCTGc                                   >  2:3815002/1‑150 (MQ=255)

                                                                                                                      tGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCACATGAATCACTCTCGTCCAGCTGCCTGTc                              >  1:1383895/1‑150 (MQ=255)

                                                                                                                      tGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTc                              <  1:455330/150‑1 (MQ=255)

                                                                                                                        gCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCa                            >  2:1185420/1‑150 (MQ=255)

                                                                                                                        gCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCa                            <  1:567834/150‑1 (MQ=255)

                                                                                                                           gCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGc                         >  2:511441/1‑150 (MQ=255)

                                                                                                                            cTCATGCACCATCACCAGCAGCCCCGGACTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCt                        >  2:1292899/1‑150 (MQ=255)

                                                                                                                               aTGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGcc                     >  2:1067113/1‑150 (MQ=255)

                                                                                                                               aTGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGcc                     >  1:4485608/1‑150 (MQ=255)

                                                                                                                                 gCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCaaa                                                         <  1:2268867/112‑1 (MQ=255)

                                                                                                                                 gCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCaaa                                                         >  2:2268867/1‑112 (MQ=255)

                                                                                                                                   aCCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCGGCAGCCTGGACAGGCAGctggac                 <  1:522605/150‑6 (MQ=255)

                                                                                                                                    ccATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGtata                <  2:1172208/150‑1 (MQ=255)

                                                                                                                                     cATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAg               >  1:4712592/1‑150 (MQ=255)

                                                                                                                                      aTCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAgc              <  2:237455/150‑1 (MQ=255)

                                                                                                                                       tCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAgcg             >  1:708835/1‑150 (MQ=255)

                                                                                                                                          tgagcagcCCGGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCa                                                               <  2:1171374/95‑1 (MQ=255)

                                                                                                                                           cagcagCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCg         <  2:1409135/150‑1 (MQ=255)

                                                                                                                                           cagcagCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCg         <  1:278128/150‑1 (MQ=255)

                                                                                                                                            agcagcCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCgg        <  1:3880259/150‑1 (MQ=255)

                                                                                                                                             gcagcCCGGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCggg       >  1:2769349/1‑150 (MQ=255)

                                                                                                                                              cagcCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGc      >  1:690627/1‑150 (MQ=255)

                                                                                                                                                 ccccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCAcg   >  1:1237780/1‑150 (MQ=255)

                                                                                                                                                 ccccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGAGCCCGGGCAcg   >  1:2638242/1‑150 (MQ=255)

                                                                                                                                                  cccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCGCCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCAcgc  <  1:3238611/150‑1 (MQ=255)

                                                                                                                                                  cccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCAcgc  <  1:1067113/150‑1 (MQ=255)

                                                                                                                                                  cccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCAcgc  >  2:5375461/1‑150 (MQ=255)

                                                                                                                                                  cccGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCAcgc  <  2:5023723/150‑1 (MQ=255)

CGACCGTCTCGTTGATCATGTCGACCACCGGCCCAGGTGTGAACCCGGTCAACAGCAGTTCCTCGGCAATCGCGTCCTGGCCGAAGTTCAGCGCAGTGATCTGGATACCGCGCCCGGCTGGCAGCTCATGCACCATCACCAGCAGCCCCGGGCTGCTCACCTCAGGTACCAGCACCTGACGGCTGGTGGCGATGCCGTAGGCCTGGCGCACAGCCAGCAGTTTCTTCACCTTGCAGGCAAATGAATCACTCTCGTCCAGCTGCCTGTCCAGGCTGCCGTATAGCGCCCGGGCACGC  >  NC_002947/3319030‑3319325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`