BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,627,632	C→T	5.4%	R279H (CGC→CAC)	PP_3196 ←	nodulation protein d1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,627,632	0	C	T	5.4%	524.7 / 12.5	167	R279H (CGC→CAC)	PP_3196	nodulation protein d1
Reads supporting (aligned to +/- strand): ref base C (71/86); new base T (7/2); total (79/88)
Fisher's exact test for biased strand distribution p-value = 8.48e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 180 total aligned reads displayed.

CGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTT  >  NC_002947/3627483‑3627780

gccatcgCCCCAGACACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtgctgc                                                                                                                                                      >  2:4088045/7‑150 (MQ=255)

gccatcgCCCCAGACACCGCAGCGGTG‑CTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtgctgc                                                                                                                                                      >  1:3164915/7‑150 (MQ=255)

cgcgATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCAtgctgc                                                                                                                                                      <  1:1031621/150‑1 (MQ=255)

 gcgATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCg                                                                                                                                                     <  1:239484/150‑1 (MQ=255)

  cgATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCgg                                                                                                                                                    <  2:3062641/150‑1 (MQ=255)

   gATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGGATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt                                                                                                                                                   >  1:4978408/1‑150 (MQ=255)

   gATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGGATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt                                                                                                                                                   >  1:4978388/1‑150 (MQ=255)

      gCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGcc                                                                                                                                                >  1:1425796/1‑150 (MQ=255)

       ccccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt                                                                                                                                                   <  1:2914/146‑1 (MQ=255)

       ccccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                               <  2:5132466/150‑1 (MQ=255)

       ccccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                               <  1:945599/150‑1 (MQ=255)

       ccccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                               >  1:2392960/1‑150 (MQ=255)

        cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                              <  1:3280169/150‑1 (MQ=255)

        cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                              >  2:2101143/1‑150 (MQ=255)

        cccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                              >  1:2366348/1‑150 (MQ=255)

         ccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcaca                                                                                                                                             >  1:1056905/1‑150 (MQ=255)

         ccAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcaca                                                                                                                                             >  2:4881885/1‑150 (MQ=255)

            g‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGTGGCACCgcagca                                                                                                                                          <  2:3770518/150‑1 (MQ=255)

              cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                         <  2:621103/150‑1 (MQ=255)

              cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                         <  2:2984284/150‑1 (MQ=255)

              cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTAGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                         <  1:1916227/150‑1 (MQ=255)

                ccGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGa                                                                                                                                       >  2:1555184/1‑150 (MQ=255)

                    tGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTc                                                                                                                                   <  1:2294510/150‑1 (MQ=255)

                      cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc                                                                                                                                 >  1:521219/1‑150 (MQ=255)

                      cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc                                                                                                                                 >  2:2440163/1‑150 (MQ=255)

                                ggCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCgg                                                                                                                       <  2:4546442/150‑1 (MQ=255)

                                ggCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCgg                                                                                                                       >  2:4568396/1‑150 (MQ=255)

                                   gATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTACGGTGCCACAGCATGAATTCACGCATAACCGGGAt                                                                                                                    <  2:2562071/150‑1 (MQ=255)

                                        ccgccgCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCa                                                                                                               <  1:3753133/150‑1 (MQ=255)

                                        ccgccgCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCa                                                                                                               >  1:4574863/1‑150 (MQ=255)

                                         cgccgcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt                                                                                                                                                   <  1:4582027/113‑1 (MQ=255)

                                         cgccgcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCAc                                                                                                              >  2:4413626/1‑150 (MQ=255)

                                          gccgcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACt                                                                                                             <  2:321317/150‑1 (MQ=255)

                                             gcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGc                                                                                                          <  2:4464868/150‑1 (MQ=255)

                                               tCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                         >  1:1349082/1‑117 (MQ=255)

                                               tCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                         <  2:1349082/117‑1 (MQ=255)

                                                cAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGt                                                                                                       <  2:2205064/150‑1 (MQ=255)

                                                 aGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCtgtg                                                                                                      >  2:982117/1‑150 (MQ=255)

                                                  ggCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTgg                                                                                                     <  2:3540609/150‑1 (MQ=255)

                                                  ggCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTgg                                                                                                     >  1:462568/1‑150 (MQ=255)

                                                       tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                <  1:1045171/150‑1 (MQ=255)

                                                       tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                <  1:1948124/150‑1 (MQ=255)

                                                       tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                <  2:4676156/150‑1 (MQ=255)

                                                               agagCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGc                                                                                        <  1:4576161/150‑1 (MQ=255)

                                                               agagCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGc                                                                                        <  1:3397171/150‑1 (MQ=255)

                                                                  gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTTATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                     <  1:4375145/150‑1 (MQ=255)

                                                                  gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                     <  1:4139659/150‑1 (MQ=255)

                                                                   cATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCa                                                                                    >  1:2785711/1‑150 (MQ=255)

                                                                       tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTc                                                                                                                                   <  2:2852007/99‑1 (MQ=255)

                                                                       tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTc                                                                                                                                   >  1:2852007/1‑99 (MQ=255)

                                                                       tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTa                                                                                >  2:4434008/1‑150 (MQ=255)

                                                                        ggCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAg                                                                               >  1:3818997/1‑150 (MQ=255)

                                                                              gTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCa                                                                         <  2:3821531/150‑1 (MQ=255)

                                                                                  tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                     <  2:3563959/150‑1 (MQ=255)

                                                                                  tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                     <  2:1360921/150‑1 (MQ=255)

                                                                                  tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                     <  1:631644/150‑1 (MQ=255)

                                                                                  tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                     <  1:5425457/150‑1 (MQ=255)

                                                                                      gcACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTc                                                                 <  2:666835/150‑1 (MQ=255)

                                                                                       cACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCg                                                                <  1:1727002/150‑1 (MQ=255)

                                                                                       cACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCg                                                                <  1:1798128/150‑1 (MQ=255)

                                                                                        aCTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCgg                                                               <  2:2473743/150‑1 (MQ=255)

                                                                                          tGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGcc                                                             >  1:990372/1‑150 (MQ=255)

                                                                                            aaTGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAg                                                           >  1:5040896/1‑150 (MQ=255)

                                                                                                 cATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTcggc                                                      <  2:827568/150‑1 (MQ=255)

                                                                                                       tGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCa                                                >  2:2199355/1‑150 (MQ=255)

                                                                                                             gcTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCAtggtgg                                          >  1:4825793/1‑150 (MQ=255)

                                                                                                              cTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGc                                         <  1:4568396/150‑1 (MQ=255)

                                                                                                              cTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGc                                         <  1:3517806/150‑1 (MQ=255)

                                                                                                                 gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                      <  2:990372/150‑1 (MQ=255)

                                                                                                                 gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                      <  2:4574863/150‑1 (MQ=255)

                                                                                                                 gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                      <  1:333397/150‑1 (MQ=255)

                                                                                                                   gAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATACCCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCCTAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAccc                                    <  2:267943/150‑1 (MQ=255)

                                                                                                                      gCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTa                                 >  2:2890471/1‑150 (MQ=255)

                                                                                                                      gCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTa                                 >  2:3283680/1‑150 (MQ=255)

                                                                                                                         aTCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACgg                              >  2:1125334/1‑150 (MQ=255)

                                                                                                                          tCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGt                             <  2:1810087/150‑1 (MQ=255)

                                                                                                                             gtgCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGcc                          <  1:420245/150‑1 (MQ=255)

                                                                                                                               gCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCcca                        <  2:2005340/150‑1 (MQ=255)

                                                                                                                                 aTGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCccacc                      >  1:3708673/1‑150 (MQ=255)

                                                                                                                                  tGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCccacca                     >  1:2653070/1‑150 (MQ=255)

                                                                                                                                   ggggTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaa                    <  2:2627222/150‑1 (MQ=255)

                                                                                                                                      gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                 >  1:400473/1‑150 (MQ=255)

                                                                                                                                      gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                 >  1:400585/1‑150 (MQ=255)

                                                                                                                                       tCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACTCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGCTAGCCGTCATAGAGTACGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCaacaac                <  1:3333199/150‑1 (MQ=255)

                                                                                                                                         gCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGTCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTg              >  2:1723287/1‑150 (MQ=255)

                                                                                                                                         gCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCa                                                                         <  2:4663561/91‑1 (MQ=255)

                                                                                                                                          ccGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgg             <  1:1629855/150‑1 (MQ=255)

                                                                                                                                          ccGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgg             >  1:2699247/1‑150 (MQ=255)

                                                                                                                                           cGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTggg            >  2:3106212/1‑150 (MQ=255)

                                                                                                                                            gTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaac                   <  2:306785/142‑1 (MQ=255)

                                                                                                                                            gTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaac                   >  1:306785/1‑142 (MQ=255)

                                                                                                                                                aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg       >  1:788157/1‑150 (MQ=255)

                                                                                                                                                aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg       >  1:1262628/1‑150 (MQ=255)

                                                                                                                                                aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg       >  1:1228455/1‑150 (MQ=255)

                                                                                                                                                aTGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg       <  1:4521109/150‑1 (MQ=255)

                                                                                                                                                 tgctgtGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAggg      >  2:4679201/1‑150 (MQ=255)

                                                                                                                                                 tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                               <  1:3503892/61‑1 (MQ=255)

                                                                                                                                                 tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                               >  2:3503892/1‑61 (MQ=255)

                                                                                                                                                  gctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGTGCAGGgt     >  2:40410/1‑150 (MQ=255)

                                                                                                                                                     gcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGtt  <  1:2136371/150‑1 (MQ=255)

CGCGATGCCCCAG‑CACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTT  >  NC_002947/3627483‑3627780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`