BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,627,635	T→C	5.9%	H278R (CAC→CGC) ‡	PP_3196 ←	nodulation protein d1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,627,635	0	T	C	5.9%	536.8 / 14.2	169	H278R (CAC→CGC) ‡	PP_3196	nodulation protein d1
Reads supporting (aligned to +/- strand): ref base T (67/92); new base C (8/2); total (75/94)
Fisher's exact test for biased strand distribution p-value = 2.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 181 total aligned reads displayed.

GATGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGAAG  >  NC_002947/3627486‑3627784

gATGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGGATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt                                                                                                                                                       >  1:4978408/1‑150 (MQ=255)

  tGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGc                                                                                                                                                     <  1:4262496/150‑1 (MQ=255)

   gCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGcc                                                                                                                                                    >  1:1425796/1‑150 (MQ=255)

    ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGt                                                                                                                                                       >  2:2914/1‑146 (MQ=255)

    ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                   <  2:5132523/150‑1 (MQ=255)

    ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                   <  1:945599/150‑1 (MQ=255)

    ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                   <  2:5132466/150‑1 (MQ=255)

    ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                   <  1:1156071/150‑1 (MQ=255)

     cccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                                  <  1:3280169/150‑1 (MQ=255)

      ccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcaca                                                                                                                                                 >  2:4881885/1‑150 (MQ=255)

      ccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcaca                                                                                                                                                 >  1:3158806/1‑150 (MQ=255)

          cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTAGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                             <  1:1916227/150‑1 (MQ=255)

            ccGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGa                                                                                                                                           >  2:1555184/1‑150 (MQ=255)

                  cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc                                                                                                                                     >  2:2440163/1‑150 (MQ=255)

                  cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc                                                                                                                                     >  1:521219/1‑150 (MQ=255)

                            ggCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCgg                                                                                                                           >  2:4568396/1‑150 (MQ=255)

                            ggCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCgg                                                                                                                           <  2:4546442/150‑1 (MQ=255)

                                    ccgccgCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCa                                                                                                                   >  1:4574863/1‑150 (MQ=255)

                                    ccgccgCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCa                                                                                                                   >  1:2603302/1‑150 (MQ=255)

                                     cgccgcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCAc                                                                                                                  >  2:4413626/1‑150 (MQ=255)

                                         gcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGc                                                                                                              <  2:4464868/150‑1 (MQ=255)

                                            cAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGt                                                                                                           <  2:2205017/150‑1 (MQ=255)

                                            cAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGt                                                                                                           >  1:3450129/1‑150 (MQ=255)

                                             aGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCtgtg                                                                                                          >  2:982117/1‑150 (MQ=255)

                                             aGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTg                                                                                                          <  1:4578921/150‑1 (MQ=255)

                                             aGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTg                                                                                                          >  1:1056441/1‑150 (MQ=255)

                                              ggCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTgg                                                                                                         <  2:3540609/150‑1 (MQ=255)

                                              ggCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTgg                                                                                                         >  1:462446/1‑150 (MQ=255)

                                                  gTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAAc                                                                                                     <  1:13427/150‑1 (MQ=255)

                                                   tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                    <  2:4676156/150‑1 (MQ=255)

                                                   tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                    <  1:1948124/150‑1 (MQ=255)

                                                   tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                    <  1:1045171/150‑1 (MQ=255)

                                                              gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                         <  1:4139797/150‑1 (MQ=255)

                                                              gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                         <  1:4375174/150‑1 (MQ=255)

                                                               cATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCa                                                                                        >  1:2785711/1‑150 (MQ=255)

                                                                   tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTc                                                                                                                                       >  1:2852007/1‑99 (MQ=255)

                                                                   tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTc                                                                                                                                       <  2:2852007/99‑1 (MQ=255)

                                                                   tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTa                                                                                    >  2:4434008/1‑150 (MQ=255)

                                                                    ggCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAg                                                                                   >  1:3818997/1‑150 (MQ=255)

                                                                          gTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCa                                                                             <  2:3821531/150‑1 (MQ=255)

                                                                              tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                         <  2:3563602/150‑1 (MQ=255)

                                                                              tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                         <  2:1360921/150‑1 (MQ=255)

                                                                              tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                         <  1:5425195/150‑1 (MQ=255)

                                                                                  gcACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTc                                                                     <  2:666835/150‑1 (MQ=255)

                                                                                   cACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCg                                                                    <  1:1727002/150‑1 (MQ=255)

                                                                                   cACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCg                                                                    <  1:1798128/150‑1 (MQ=255)

                                                                                    aCTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCgg                                                                   <  2:2473743/150‑1 (MQ=255)

                                                                                      tGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGcc                                                                 >  1:990372/1‑150 (MQ=255)

                                                                                        aaTGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAg                                                               >  1:5040896/1‑150 (MQ=255)

                                                                                             cATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTcggc                                                          <  2:827568/150‑1 (MQ=255)

                                                                                              aTCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGAGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTcggcg                                                         >  1:2192710/1‑150 (MQ=255)

                                                                                                   tGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCa                                                    >  2:2174639/1‑150 (MQ=255)

                                                                                                         gcTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCAtggtgg                                              <  2:106379/150‑1 (MQ=255)

                                                                                                          cTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGc                                             <  1:3517806/150‑1 (MQ=255)

                                                                                                          cTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGc                                             <  1:4568396/150‑1 (MQ=255)

                                                                                                           tCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGTGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCg                                            >  2:2250070/1‑150 (MQ=255)

                                                                                                           tCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCg                                            >  1:2366267/1‑150 (MQ=255)

                                                                                                           tCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCg                                            >  1:2366439/1‑150 (MQ=255)

                                                                                                            cgcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGa                                           <  1:4850410/150‑1 (MQ=255)

                                                                                                             gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                          <  2:4574863/150‑1 (MQ=255)

                                                                                                             gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                          <  2:990372/150‑1 (MQ=255)

                                                                                                             gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                          >  1:1450083/1‑150 (MQ=255)

                                                                                                             gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                          <  1:333423/150‑1 (MQ=255)

                                                                                                               gAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATACCCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCCTAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAccc                                        <  2:267943/150‑1 (MQ=255)

                                                                                                                  gCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTa                                     >  2:3283680/1‑150 (MQ=255)

                                                                                                                    cATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACg                                   >  1:620863/1‑150 (MQ=255)

                                                                                                                      tCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGt                                 <  2:1810087/150‑1 (MQ=255)

                                                                                                                          tgCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCAGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGccc                             >  2:1326870/1‑150 (MQ=255)

                                                                                                                           gCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCcca                            >  2:3001764/1‑150 (MQ=255)

                                                                                                                           gCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCcca                            <  2:2005340/150‑1 (MQ=255)

                                                                                                                             aTGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCccacc                          >  1:3708673/1‑150 (MQ=255)

                                                                                                                             aTGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCccacc                          <  2:3102436/150‑1 (MQ=255)

                                                                                                                              tGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCccacca                         >  1:2653070/1‑150 (MQ=255)

                                                                                                                                  gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                     >  2:3615968/1‑150 (MQ=255)

                                                                                                                                  gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                     >  1:400473/1‑150 (MQ=255)

                                                                                                                                  gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                     >  1:400524/1‑150 (MQ=255)

                                                                                                                                   tCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACTCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGCTAGCCGTCATAGAGTACGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCaacaac                    <  1:3333199/150‑1 (MQ=255)

                                                                                                                                     gCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGTCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTg                  >  2:1723287/1‑150 (MQ=255)

                                                                                                                                     gCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTg                  <  2:3158806/150‑1 (MQ=255)

                                                                                                                                     gCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGAAGCCGGCa                                                                             >  1:4663561/1‑91 (MQ=255)

                                                                                                                                      ccGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgg                 <  1:1629855/150‑1 (MQ=255)

                                                                                                                                      ccGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgg                 >  1:2699247/1‑150 (MQ=255)

                                                                                                                                        gTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaac                       <  2:306785/142‑1 (MQ=255)

                                                                                                                                            aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg           >  1:1228621/1‑150 (MQ=255)

                                                                                                                                            aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg           >  1:788157/1‑150 (MQ=255)

                                                                                                                                            aTGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg           <  1:4521109/150‑1 (MQ=255)

                                                                                                                                            aTGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg           >  2:2673670/1‑150 (MQ=255)

                                                                                                                                             tgctgtGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAggg          >  2:4679201/1‑150 (MQ=255)

                                                                                                                                             tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                                   >  2:3503892/1‑61 (MQ=255)

                                                                                                                                             tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                                   <  1:3503892/61‑1 (MQ=255)

                                                                                                                                              gctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGTGCAGGgt         >  2:40410/1‑150 (MQ=255)

                                                                                                                                                 gcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGtt      <  1:3000704/150‑1 (MQ=255)

                                                                                                                                                 gcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGtt      <  2:2603302/150‑1 (MQ=255)

                                                                                                                                                   ggTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGa    <  2:1664996/150‑1 (MQ=255)

                                                                                                                                                    gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa   <  2:462446/150‑1 (MQ=255)

                                                                                                                                                    gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa   <  2:462568/150‑1 (MQ=255)

                                                                                                                                                    gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa   <  2:2366267/150‑1 (MQ=255)

                                                                                                                                                    gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa   <  1:404345/150‑1 (MQ=255)

                                                                                                                                                    gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa   <  1:4676619/150‑1 (MQ=255)

                                                                                                                                                     tGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGAAg  <  1:3106212/150‑1 (MQ=255)

GATGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGAAG  >  NC_002947/3627486‑3627784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`