BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,627,636	G→A	5.8%	H278Y (CAC→TAC) ‡	PP_3196 ←	nodulation protein d1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,627,636	0	G	A	5.8%	533.8 / 14.0	171	H278Y (CAC→TAC) ‡	PP_3196	nodulation protein d1
Reads supporting (aligned to +/- strand): ref base G (67/94); new base A (8/2); total (75/96)
Fisher's exact test for biased strand distribution p-value = 2.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 172 total aligned reads displayed.

TGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGAAGT  >  NC_002947/3627488‑3627785

tGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGc                                                                                                                                                      <  1:4262496/150‑1 (MQ=255)

  ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                    <  1:1156071/150‑1 (MQ=255)

  ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                    <  2:5132523/150‑1 (MQ=255)

  ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                    <  2:4938834/150‑1 (MQ=255)

  ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                    >  1:267943/1‑150 (MQ=255)

  ccccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCca                                                                                                                                                    >  1:2392960/1‑150 (MQ=255)

   cccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                                   <  1:3280169/150‑1 (MQ=255)

   cccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                                   >  2:2101143/1‑150 (MQ=255)

   cccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcac                                                                                                                                                   >  1:2366348/1‑150 (MQ=255)

    ccAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCcaca                                                                                                                                                  >  1:3158806/1‑150 (MQ=255)

        cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                              <  2:2984284/150‑1 (MQ=255)

        cACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                              <  2:672802/150‑1 (MQ=255)

            gCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAAt                                                                                                                                          <  2:941058/150‑1 (MQ=255)

               gCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCa                                                                                                                                       <  1:2825633/150‑1 (MQ=255)

               gCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCa                                                                                                                                       <  2:1425796/150‑1 (MQ=255)

                cGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCAc                                                                                                                                      >  1:521219/1‑150 (MQ=255)

                          ggCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCgg                                                                                                                            >  2:4568256/1‑150 (MQ=255)

                                  ccgccgCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCa                                                                                                                    <  1:3753133/150‑1 (MQ=255)

                                  ccgccgCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCa                                                                                                                    >  1:4574863/1‑150 (MQ=255)

                                    gccgcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACt                                                                                                                  <  2:321317/150‑1 (MQ=255)

                                       gcTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGc                                                                                                               <  2:4464868/150‑1 (MQ=255)

                                         tCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                              <  2:1349082/117‑1 (MQ=255)

                                         tCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCAt                                                                                                                                              >  1:1349082/1‑117 (MQ=255)

                                          cAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGt                                                                                                            <  2:1573782/150‑1 (MQ=255)

                                          cAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGt                                                                                                            <  2:2205064/150‑1 (MQ=255)

                                           aGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTg                                                                                                           <  1:4578921/150‑1 (MQ=255)

                                           aGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTg                                                                                                           >  1:1056441/1‑150 (MQ=255)

                                            ggCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTgg                                                                                                          <  2:3540609/150‑1 (MQ=255)

                                            ggCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTgg                                                                                                          >  1:462446/1‑150 (MQ=255)

                                                 tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                     <  1:1948124/150‑1 (MQ=255)

                                                 tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                     <  1:1045171/150‑1 (MQ=255)

                                                 tGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACa                                                                                                     <  2:4676156/150‑1 (MQ=255)

                                                         agagCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGc                                                                                             <  1:3397171/150‑1 (MQ=255)

                                                            gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                          <  1:4139659/150‑1 (MQ=255)

                                                            gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                          <  1:4139797/150‑1 (MQ=255)

                                                            gCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGc                                                                                          <  1:4375174/150‑1 (MQ=255)

                                                             cATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCa                                                                                         >  1:2785602/1‑150 (MQ=255)

                                                                 tGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTc                                                                                                                                        <  2:2852007/99‑1 (MQ=255)

                                                                        gTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCa                                                                              <  2:3821531/150‑1 (MQ=255)

                                                                            tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                          <  1:5003362/150‑1 (MQ=255)

                                                                            tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                          <  1:5425457/150‑1 (MQ=255)

                                                                            tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                          <  2:3563602/150‑1 (MQ=255)

                                                                            tGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATaga                                                                          <  1:631644/150‑1 (MQ=255)

                                                                                gcACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTc                                                                      <  2:4494894/150‑1 (MQ=255)

                                                                                gcACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTc                                                                      <  2:666835/150‑1 (MQ=255)

                                                                                 cACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCg                                                                     <  1:1798128/150‑1 (MQ=255)

                                                                                 cACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCg                                                                     <  1:1727002/150‑1 (MQ=255)

                                                                                  aCTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCgg                                                                    <  2:2473743/150‑1 (MQ=255)

                                                                                    tGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGcc                                                                  >  1:990315/1‑150 (MQ=255)

                                                                                      aaTGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAg                                                                >  1:5040896/1‑150 (MQ=255)

                                                                                            aTCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGAGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTcggcg                                                          >  1:2192710/1‑150 (MQ=255)

                                                                                                 tGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCa                                                     >  2:2199355/1‑150 (MQ=255)

                                                                                                    tGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCAtgg                                                  <  1:3886797/150‑1 (MQ=255)

                                                                                                       gcTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCAtggtgg                                               >  1:4825793/1‑150 (MQ=255)

                                                                                                       gcTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCAtggtgg                                               <  2:106379/150‑1 (MQ=255)

                                                                                                        cTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGc                                              <  1:4568396/150‑1 (MQ=255)

                                                                                                        cTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGc                                              <  1:4568256/150‑1 (MQ=255)

                                                                                                         tCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCg                                             >  1:2366267/1‑150 (MQ=255)

                                                                                                           gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                           >  1:1450083/1‑150 (MQ=255)

                                                                                                           gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                           <  2:990315/150‑1 (MQ=255)

                                                                                                           gcgAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAc                                           <  1:333397/150‑1 (MQ=255)

                                                                                                             gAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATACCCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCCTAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGAccc                                         <  2:267943/150‑1 (MQ=255)

                                                                                                                gCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTa                                      >  2:3283680/1‑150 (MQ=255)

                                                                                                                   aTCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACgg                                   >  2:1125334/1‑150 (MQ=255)

                                                                                                                    tCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGt                                  <  2:1810087/150‑1 (MQ=255)

                                                                                                                       gtgCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGcc                               <  1:1853975/150‑1 (MQ=255)

                                                                                                                         gCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGcccc                             >  1:363335/1‑149 (MQ=255)

                                                                                                                         gCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCcca                             <  2:2005340/150‑1 (MQ=255)

                                                                                                                         gCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCcca                             >  2:3001764/1‑150 (MQ=255)

                                                                                                                            tGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCccacca                          >  1:2653070/1‑150 (MQ=255)

                                                                                                                             ggggTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaa                         <  2:2627222/150‑1 (MQ=255)

                                                                                                                                gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                      >  2:3615968/1‑150 (MQ=255)

                                                                                                                                gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                      >  1:400473/1‑150 (MQ=255)

                                                                                                                                gTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaacaa                      >  1:400585/1‑150 (MQ=255)

                                                                                                                                 tCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACTCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGCTAGCCGTCATAGAGTACGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCaacaac                     <  1:3333199/150‑1 (MQ=255)

                                                                                                                                   gCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGAAGCCGGCa                                                                              >  1:4663561/1‑91 (MQ=255)

                                                                                                                                    ccGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgg                  <  1:1629855/150‑1 (MQ=255)

                                                                                                                                     cGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTggg                 >  2:3106212/1‑150 (MQ=255)

                                                                                                                                      gTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaac                        <  2:306785/142‑1 (MQ=255)

                                                                                                                                      gTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACcaac                        >  1:306785/1‑142 (MQ=255)

                                                                                                                                      attcATGCTGTGGCACCGCAGCATGGATTCACGCATAACCGGGATCTTCACTGGCGGTGGCACCAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgggg                >  2:4318059/4‑150 (MQ=255)

                                                                                                                                      attcATGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTgggg                >  2:48339/4‑150 (MQ=255)

                                                                                                                                       ttcATGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGt                                  >  2:4570061/3‑131 (MQ=255)

                                                                                                                                          aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg            >  1:788157/1‑150 (MQ=255)

                                                                                                                                          aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg            >  1:1228621/1‑150 (MQ=255)

                                                                                                                                          aTGCTGTGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg            >  1:1262628/1‑150 (MQ=255)

                                                                                                                                          aTGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAgg            >  2:4430218/1‑150 (MQ=255)

                                                                                                                                           tgctgtGGCACCGCAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAggg           >  2:4679201/1‑150 (MQ=255)

                                                                                                                                           tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                                    <  1:3503892/61‑1 (MQ=255)

                                                                                                                                           tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                                    <  1:3503929/61‑1 (MQ=255)

                                                                                                                                           tgctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAg                                                                                                    >  2:3503892/1‑61 (MQ=255)

                                                                                                                                            gctgcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGTGCAGGgt          >  2:40410/1‑150 (MQ=255)

                                                                                                                                               gcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGtt       <  2:2603302/150‑1 (MQ=255)

                                                                                                                                               gcGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGtt       <  1:3000704/150‑1 (MQ=255)

                                                                                                                                                 ggTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGa     <  2:1664996/150‑1 (MQ=255)

                                                                                                                                                  gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa    <  2:2366267/150‑1 (MQ=255)

                                                                                                                                                  gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa    <  2:2366439/150‑1 (MQ=255)

                                                                                                                                                  gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa    <  2:462446/150‑1 (MQ=255)

                                                                                                                                                  gTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGaa    <  2:462568/150‑1 (MQ=255)

                                                                                                                                                   tGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGAAg   <  1:1623464/150‑1 (MQ=255)

TGCCCCAGCACCGCTGCGGTGTCTGGGGCGATGGCCGCCGCTCAGGCGGTGGCGCGCAGAGCATCTGGCTGCGTGCTGGCGCACTGAATGACATCGCTGATGCGCTCGCGAAGCCATCTGTGCATGGGGTCGCCGTCCATGCTGCGGTGCCACAGCATGAATTCACGCATAACCGGGATCTTCACTGGCGGTGGCAACAGGCGCAGCGGCAGGTAGCCGGCATAGAGTTCGGCCAGTCGGCGGTGCATGGTGGCGACCCGTACGGTGCCCACCAACAACTGGGGCAGGGTGTTGAAGT  >  NC_002947/3627488‑3627785

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`