Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 258,536 A→C 6.2% I24L (ATA→CTA)  PP_0207 → nitrate ABC transporter substrate‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947258,5360AC 6.2% 523.8 / 2.8 176I24L (ATA→CTA) PP_0207nitrate ABC transporter substrate‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (72/89);  new base C (10/5);  total (82/94)
Fisher's exact test for biased strand distribution p-value = 1.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 182 total aligned reads displayed.

CTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACC  >  NC_002947/258387‑258685
                                                                                                                                                     |                                                                                                                                                     
ctgTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCa                                                                                                                                                       <  1:3308452/150‑1 (MQ=255)
ctgTGCCCCTCGTTTAGCACCACGCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGATACACCCATGCGCCTTGTTGCATCCGTGGCCGGACTCGCGCTGGCGCTCTGTGGCCTGTACGCCAGGGCCGATACCa                                                                                                                                                       <  2:3115594/150‑1 (MQ=255)
  gtgCACCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATa                                                                                                                                                     <  2:4788393/150‑1 (MQ=255)
   tgCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATAc                                                                                                                                                    <  2:1322337/150‑1 (MQ=255)
     ccccTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTTGCCTTTACGCCAGCGCCGAAAACATActc                                                                                                                                                  >  1:4941375/1‑148 (MQ=255)
      cccTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCa                                                                                                                                                 <  2:4165356/150‑1 (MQ=255)
      cccTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCa                                                                                                                                                 <  1:3035321/150‑1 (MQ=255)
      cccTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATAAGCa                                                                                                                                                 >  2:3997243/1‑150 (MQ=255)
       ccTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCGGAAACCCACCGCCGAAAACAAACGCaa                                                                                                                                                >  1:1412608/1‑149 (MQ=255)
        cTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGGGCGGGCGCGCACTGGCCGGTAAGCCAGCCCCCCAACCCAACGCATc                                                                                                                                               >  1:714435/1‑150 (MQ=255)
             tccccACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCGTGTTGCAACCGTGGCCGGCCTCGCGCGGGCGCGCACTGGCCGGACCGCCCGCGCCGAAACCATACGCAGCGccct                                                                                                                                          >  2:4083512/3‑148 (MQ=255)
               cccACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCg                                                                                                                                        <  2:3040012/150‑1 (MQ=255)
                 caccacCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGc                                                                                                                                      >  2:4212731/1‑150 (MQ=255)
                 caccacCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGc                                                                                                                                      <  1:2749302/150‑1 (MQ=255)
                   ccaccCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCGGAACGCCAGCGCCGAAACCAAAAGCATCCCCAGAGGCAc                                                                                                                                    >  1:510163/1‑150 (MQ=255)
                     accCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCGGAACGCCAGCGCCGAAACCAGACGCATCGCCAGCGGCAccc                                                                                                                                  >  1:3625860/1‑150 (MQ=255)
                     accCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCTGAACGCCAGCGCCGAAACCCAACGCAACGCCCACGGCAccc                                                                                                                                  >  1:376414/1‑150 (MQ=255)
                     accCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCGCACTGGCCGGAACGCCACCGCCGAAACCAAACGCATCGCCAACGGCAccc                                                                                                                                  >  2:690789/1‑150 (MQ=255)
                        cAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAgg                                                                                                                               <  1:773215/150‑1 (MQ=255)
                         aaGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGa                                                                                                                              <  2:3172181/150‑1 (MQ=255)
                            ccGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGacac                                                                                                                           <  2:1734566/150‑1 (MQ=255)
                                     ggAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCaa                                                                                                                  <  1:407051/150‑1 (MQ=255)
                                     ggAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCaa                                                                                                                  >  2:1687030/1‑150 (MQ=255)
                                      gagaCGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCGGGCGCGCACTGGGCAGAAAGCCAGCCCCGAAAACAAACCCAACGCCAAACGCACCCACGACACCACCCACAAc                                                                                                                 >  1:4614480/1‑150 (MQ=255)
                                           gAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATAGGCACCCAGGACACCACCAACAACAgcgc                                                                                                            >  2:2024463/1‑150 (MQ=255)
                                              gCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCTGAACGCCACCGCCCAAACCAAAACCAACCCCAACGGCACCCCCGACACCACCAACAACAGcccccc                                                                                                         >  1:5224090/1‑148 (MQ=255)
                                                cAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCGACTGCGCCACCg                                                                                                       <  2:1884498/150‑1 (MQ=255)
                                                cAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCg                                                                                                       <  2:5160964/150‑1 (MQ=255)
                                                cAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGGGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCGACTGCGCCACCg                                                                                                       <  2:2637039/150‑1 (MQ=255)
                                                 aaCTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCgg                                                                                                      <  1:1659055/150‑1 (MQ=255)
                                                  aCTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGt                                                                                                     <  2:105833/150‑1 (MQ=255)
                                                  aCTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCTGAAACCCCCCGCCCAAAACAAAAGCATCGCCATCGGCACCCAGGACACCACCAACCACGGCGCCCCCCGt                                                                                                     >  2:1520077/1‑150 (MQ=255)
                                                    tctTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGGGCGGAAAGGCCGGGAAGCCAGCCCCGAAACCATAACCAACGCCCACCGCACCCCGGCCACCCCCAACAAAAGCGCcccccgggg                                                                                                   >  1:2429104/1‑145 (MQ=255)
                                                    tctTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTgg                                                                                                   <  2:1746541/150‑1 (MQ=255)
                                                    tctTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACAGCGCCACCCGTgg                                                                                                   >  2:4134212/1‑150 (MQ=255)
                                                    tctTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCAGAAAGCCAGCGCCGAAACCATACGCAACGCCATCGGCAACCAGGACACCACCATCAACAGCGCCACCCGTgg                                                                                                   >  1:440058/1‑150 (MQ=255)
                                                    tctTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCGCACAGGCCTGAAAGCCCGCGCCCAAACCCAAAGCAACGCCATCGGCACCCACGACACCACCAACAACAGCGCCAcccgagg                                                                                                   >  1:4349600/1‑145 (MQ=255)
                                                     ctTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGc                                                                                                  <  1:1941321/150‑1 (MQ=255)
                                                      ttATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGcc                                                                                                 <  2:4935669/150‑1 (MQ=255)
                                                      ttATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGcc                                                                                                 <  1:744118/150‑1 (MQ=255)
                                                      ttATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCAGAACCCCAGCGCCGAAACCATACCCATCGCCCACCGCACCCAGGACACCACCAACAACAGCGCCACCCGTGGcc                                                                                                 >  2:3856753/1‑150 (MQ=255)
                                                        ataACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCctg                                                                                               <  1:1247358/150‑1 (MQ=255)
                                                         taACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCctgc                                                                                              >  2:4639282/1‑150 (MQ=255)
                                                           aCGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCctgctg                                                                                            >  2:5112738/1‑150 (MQ=255)
                                                              aTTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATc                                                                                         <  1:4639282/150‑1 (MQ=255)
                                                                   aGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGa                                                                                    <  1:5331471/150‑1 (MQ=255)
                                                                   aGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGa                                                                                    <  2:4592970/150‑1 (MQ=255)
                                                                     gggAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCGGAACGCCCGCGCCGCAACCCTACGCCACGCCCACGGCACCCACGCCCCCCCCCACAAAAGCGCCACCCCTGGCCTGCTGCTCCgggggc                                                                                  >  1:5192784/1‑145 (MQ=255)
                                                                     gggAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACAGGCCGGAAAGCCAGCCCCGAAAACATACGCATCGCCAACGGCACCCACGACCCCACCAACAACAGCGCCACCCGTGGCCTGCTGATACGGGAGc                                                                                  >  1:2696571/1‑150 (MQ=255)
                                                                        aaaCACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTgg                                                                               <  1:2583168/150‑1 (MQ=255)
                                                                              ccATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCAACGCCATCGGCACCCAGGACCCCACCCTCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCctgc                                                                         >  2:1242570/1‑150 (MQ=255)
                                                                               cATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCctgct                                                                        <  1:4023793/150‑1 (MQ=255)
                                                                                aTGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCctgctg                                                                       <  1:5083805/150‑1 (MQ=255)
                                                                                  gcgcCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCCTACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGa                                                                     <  2:4350852/150‑1 (MQ=255)
                                                                                    gcCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCCTACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACa                                                                   <  1:1854916/150‑1 (MQ=255)
                                                                                     ccTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACaa                                                                  <  2:922313/150‑1 (MQ=255)
                                                                                      cTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCAACGCCATCGGCACCCAGGACACCACCAACAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAg                                                                 >  2:2708210/1‑150 (MQ=255)
                                                                                           tgCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCt                                                            <  2:2379869/150‑1 (MQ=255)
                                                                                           tgCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCt                                                            <  2:4712178/150‑1 (MQ=255)
                                                                                              aaCCGTGGCCGGCCTCGCGCAGGCGCTCACAGGCCTGAAAGCCAGCGCCCAAACCAAACGCAACGCCATCCGCACCCCCGACACCACCATACACATCGCCACCGGTGGCCTGCAGATCCGTGAGCTGGGCCTGCTGGACAAGTACCAGcc                                                         >  2:5232947/1‑150 (MQ=255)
                                                                                                          ccTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCaa                                             <  1:1520077/150‑1 (MQ=255)
                                                                                                          ccTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCaa                                             >  2:354302/1‑150 (MQ=255)
                                                                                                            tCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACCCCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGt                                           >  2:3384410/1‑150 (MQ=255)
                                                                                                            tCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGt                                           >  1:780486/1‑150 (MQ=255)
                                                                                                             cgcgCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCa                                                      <  2:1230471/138‑1 (MQ=255)
                                                                                                             cgcgCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCa                                                      >  1:1230471/1‑138 (MQ=255)
                                                                                                                gcTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACaa                                       <  2:5134094/150‑1 (MQ=255)
                                                                                                                 cTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAg                                      <  1:2817448/150‑1 (MQ=255)
                                                                                                                  tGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAgg                                     >  1:4895525/1‑150 (MQ=255)
                                                                                                                    gcgcTCACTGGCCTGAACGCCGGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGAc                                   <  2:565014/150‑1 (MQ=255)
                                                                                                                    gcgcTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGAc                                   >  2:1156877/1‑150 (MQ=255)
                                                                                                                    gcgcTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGAc                                   <  1:3555267/150‑1 (MQ=255)
                                                                                                                      gcTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGt                                           <  2:4031540/140‑1 (MQ=255)
                                                                                                                      gcTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGc                                 >  1:914028/1‑150 (MQ=255)
                                                                                                                      gcTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGc                                 <  1:2041217/150‑1 (MQ=255)
                                                                                                                          aCTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCAcc                             >  1:2332667/1‑150 (MQ=255)
                                                                                                                          aCTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCAcc                             >  1:2248186/1‑150 (MQ=255)
                                                                                                                             ggCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTAc                          <  2:2065465/150‑1 (MQ=255)
                                                                                                                                cTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAg                       >  2:3079130/1‑150 (MQ=255)
                                                                                                                                cTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAg                       >  1:188070/1‑150 (MQ=255)
                                                                                                                                 tGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAgg                      >  1:3200152/1‑150 (MQ=255)
                                                                                                                                  gAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGt                     >  1:427968/1‑150 (MQ=255)
                                                                                                                                   aaCGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTc                    <  2:3510827/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGt                <  1:454006/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGt                <  2:2520102/150‑1 (MQ=255)
                                                                                                                                       ccAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGt                <  1:4212731/150‑1 (MQ=255)
                                                                                                                                        cAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACAATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTg               >  2:2935062/1‑150 (MQ=255)
                                                                                                                                          gcgcCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGga             >  1:3920347/1‑150 (MQ=255)
                                                                                                                                             ccGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGgaaga          >  1:4695843/1‑150 (MQ=255)
                                                                                                                                             ccGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGgaaga          <  1:2453695/150‑1 (MQ=255)
                                                                                                                                             ccGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGgaaga          <  2:376414/150‑1 (MQ=255)
                                                                                                                                              cGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGgaagaa         <  2:4759572/150‑1 (MQ=255)
                                                                                                                                               gAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAAc        >  1:2162154/1‑150 (MQ=255)
                                                                                                                                               gAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAAc        <  2:4502478/150‑1 (MQ=255)
                                                                                                                                                  aCCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTc     <  2:714435/150‑1 (MQ=255)
                                                                                                                                                  aCCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTc     <  1:4088314/150‑1 (MQ=255)
                                                                                                                                                    cATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCAc   <  1:61616/150‑1 (MQ=255)
                                                                                                                                                    cATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCAc   <  2:4533415/150‑1 (MQ=255)
                                                                                                                                                     aTACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCAcc  >  2:21729/1‑150 (MQ=255)
                                                                                                                                                     aTACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCAcc  >  2:807297/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGTGCCCCTCGTTTCCCACCACCCAAGCCGGGCGGCGGAGACGAAGCCAACTCTTATAACGATTCGAGGGGAAACACCCATGCGCCTTGTTGCAACCGTGGCCGGCCTCGCGCTGGCGCTCACTGGCCTGAACGCCAGCGCCGAAACCATACGCATCGCCATCGGCACCCAGGACACCACCATCAACTGCGCCACCGGTGGCCTGCTGATCCGTGAGCTGGGCCTGCTGGACAAGTACCTGCCGCACGACGGCAAGTACAAGGACGCCACCTACCAGGTCGAGTGGAAGAACTTCACC  >  NC_002947/258387‑258685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑