BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,699,409	G→A	5.2%	S15S (AGC→AGT) ‡	PP_5595 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,699,409	0	G	A	5.2%	608.6 / 14.3	192	S15S (AGC→AGT) ‡	PP_5595	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (97/85); new base A (5/5); total (102/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01

Only 100 of 194 total aligned reads displayed.

GCATGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGCC  >  NC_002947/3699261‑3699557

gCATGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGc                                                                                                                                                      >  1:3199252/1‑150 (MQ=255)

  aTGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTg                                                                                                                                                    <  2:4759836/150‑1 (MQ=255)

  aTGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCGCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTg                                                                                                                                                    <  1:273988/150‑1 (MQ=255)

      ccTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCa                                                                                                                                                <  1:3381315/150‑1 (MQ=255)

      ccTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCa                                                                                                                                                <  2:4998769/150‑1 (MQ=255)

       cTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAg                                                                                                                                               >  1:2261537/1‑150 (MQ=255)

        ttGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAgg                                                                                                                                              >  1:966958/1‑150 (MQ=255)

        ttGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGATATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAgg                                                                                                                                              >  2:2112461/1‑150 (MQ=255)

         tGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑c                                                                                                                                            >  2:1031255/1‑150 (MQ=255)

         tGCTGTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGTGTATGTGGCGGTAGCTGTAACCGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCAGCGGCCAggt                                                                                                                                             <  2:3452467/150‑2 (MQ=255)

          gCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑Ct                                                                                                                                           >  1:4153724/1‑150 (MQ=255)

           cTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTg                                                                                                                                          >  1:2539703/1‑150 (MQ=255)

            tttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTgg                                                                                                                                         >  2:310045/1‑150 (MQ=255)

            tttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAG‑CCTGGCCAGCGGCCAggtctgg                                                                                                                                         <  2:797629/150‑6 (MQ=255)

            tttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAG‑CCTGGCCAGCGGCCAggtctgg                                                                                                                                         <  2:4922797/150‑6 (MQ=255)

             ttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGc                                                                                                                                        <  2:955648/150‑1 (MQ=255)

                  tgctgcATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACca                                                                                                                                   <  2:3212560/150‑1 (MQ=255)

                    ctgcATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatc                                                                                                                                 <  1:2466386/150‑1 (MQ=255)

                    ctgcATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatc                                                                                                                                 <  1:4967195/150‑1 (MQ=255)

                            ccAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGTCCGCTGGCCAGG‑CTGGCAACCATCATCATCaa                                                                                                                         <  2:5231826/150‑1 (MQ=255)

                               gTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGc                                                                                                                      <  2:538506/150‑1 (MQ=255)

                                   aTCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGa                                                                                                                  >  1:2062475/1‑150 (MQ=255)

                                    tCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCACCCATCATCATCAAGGCAGGaa                                                                                                                 >  2:543831/1‑150 (MQ=255)

                                    tCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGac                                                                                                                 >  2:4350378/1‑149 (MQ=255)

                                     cGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCatcatcatc                                                                                                                           <  1:1789074/139‑1 (MQ=255)

                                     cGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAac                                                                                                                <  1:1427341/150‑1 (MQ=255)

                                       cTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAacac                                                                                                              >  2:1739394/1‑150 (MQ=255)

                                       cTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAacac                                                                                                              <  1:4460026/150‑1 (MQ=255)

                                       cTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAacac                                                                                                              >  2:1689652/1‑150 (MQ=255)

                                        ttCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAg                                                                                                                    >  1:5216127/1‑143 (MQ=255)

                                                      tGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTcc                                                                                               >  1:3717361/1‑150 (MQ=255)

                                                         tgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                            <  1:3727121/150‑1 (MQ=255)

                                                         tgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                            >  1:1960970/1‑150 (MQ=255)

                                                          gtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAggg                                                                                           >  1:114605/1‑150 (MQ=255)

                                                               tgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACaa                                                                                      >  2:3164083/1‑150 (MQ=255)

                                                                gtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAg                                                                                     <  2:4153724/150‑1 (MQ=255)

                                                                     aGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCt                                                                                >  1:930701/1‑150 (MQ=255)

                                                                       gtgtGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCTTTTTCCACCTCCAGGGCACAAGGCCCTCg                                                                              >  1:1485113/1‑150 (MQ=255)

                                                                         gtgCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCACCCCTCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTc                                                                            >  1:1606760/1‑150 (MQ=255)

                                                                          tgCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCACCCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCa                                                                           >  1:2268233/1‑150 (MQ=255)

                                                                           gCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAg                                                                          <  2:4958512/150‑1 (MQ=255)

                                                                            tcGCTCCTCCAGCGACGCCGTGACGCCGGTGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                         >  2:4283673/2‑150 (MQ=38)

                                                                            ccGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                         >  1:3519266/1‑150 (MQ=255)

                                                                            ccGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                         >  2:2542498/1‑150 (MQ=255)

                                                                             cGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGt                                                                             <  1:4050859/145‑1 (MQ=255)

                                                                             cGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGt                                                                             >  2:4050859/1‑145 (MQ=255)

                                                                                 ccACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGa                                                                    <  1:3094994/150‑1 (MQ=255)

                                                                                 ccACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGa                                                                    >  1:67285/1‑150 (MQ=255)

                                                                                  cACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAg                                                                   >  1:3602785/1‑150 (MQ=255)

                                                                                  cACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAg                                                                   >  2:2849762/1‑150 (MQ=255)

                                                                                     cGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAg                                                                <  1:574923/150‑1 (MQ=255)

                                                                                       gCGGCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑c                                                                                                                                            >  1:698345/1‑72 (MQ=255)

                                                                                            aCGGCGTCGCTGGAGGAGCGAGTCAGCACATAGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTc                                                         >  2:1126382/1‑150 (MQ=255)

                                                                                             cGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTcg                                                        >  2:5312406/1‑150 (MQ=255)

                                                                                                    gCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGgc                                                 <  2:3043965/150‑1 (MQ=255)

                                                                                                      tGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGgcgc                                               >  2:3231754/1‑150 (MQ=255)

                                                                                                      tGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGgcgc                                               >  2:2899375/1‑150 (MQ=255)

                                                                                                      tGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CCGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACaa                                                                                      <  1:5273010/111‑1 (MQ=255)

                                                                                                      tGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CCGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACaa                                                                                      >  2:5273010/1‑111 (MQ=255)

                                                                                                           gagCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGc                                          >  2:1436898/1‑150 (MQ=255)

                                                                                                              cGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                       >  2:469663/1‑150 (MQ=255)

                                                                                                              cGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                       <  1:310045/150‑1 (MQ=255)

                                                                                                              cGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCGCCAGGGCACACGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                       <  2:4581529/150‑1 (MQ=255)

                                                                                                               gAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTg                                      <  1:1339776/150‑1 (MQ=255)

                                                                                                               gAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTg                                      <  1:3994408/150‑1 (MQ=255)

                                                                                                                 gTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCCGGCTTGTCGCGCCGGCGCATGGCTTTGgc                                    >  2:4442687/1‑150 (MQ=255)

                                                                                                                  tCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGgcg                                   >  1:529184/1‑150 (MQ=255)

                                                                                                                    aGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGcgcg                                 <  1:3348409/150‑1 (MQ=255)

                                                                                                                     gCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGt                                >  2:5136328/1‑150 (MQ=255)

                                                                                                                      cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGtt                               >  1:3041548/1‑150 (MQ=255)

                                                                                                                      cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGtt                               >  2:4894661/1‑150 (MQ=255)

                                                                                                                      cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTGTGGCGCGtt                               >  2:3507905/1‑150 (MQ=255)

                                                                                                                       acaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGttt                              <  1:583982/150‑1 (MQ=255)

                                                                                                                         aTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTc                                                                                                <  1:5249726/82‑1 (MQ=255)

                                                                                                                          ttGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTc                           >  2:2683672/1‑150 (MQ=255)

                                                                                                                           tGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTcc                          <  1:2899375/150‑1 (MQ=255)

                                                                                                                           tGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTcc                          >  1:5419579/1‑150 (MQ=255)

                                                                                                                           tGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTcc                          >  1:2696121/1‑150 (MQ=255)

                                                                                                                            gCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                         <  1:4026793/150‑1 (MQ=255)

                                                                                                                              aaGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAgg                       <  1:4875693/150‑1 (MQ=255)

                                                                                                                                gCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGAt                     >  1:4517789/1‑150 (MQ=255)

                                                                                                                                gCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGAt                     <  1:147595/150‑1 (MQ=255)

                                                                                                                                gCTTCGGCCCAGACCGGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGAt                     >  2:4552516/1‑150 (MQ=255)

                                                                                                                                 cTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATc                    >  2:5089358/1‑150 (MQ=255)

                                                                                                                                 cTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATc                    >  1:5130018/1‑150 (MQ=255)

                                                                                                                                 cTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATc                    >  1:5130261/1‑150 (MQ=255)

                                                                                                                                     ggCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggt                <  1:1129129/150‑1 (MQ=255)

                                                                                                                                        ccAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtagg             >  1:1024291/1‑150 (MQ=255)

                                                                                                                                        ccAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCGTGTCGCGCCGGCGCATGGCTGTGGCGCGTTTTTCCAGGATCaggtagg             >  2:1649507/1‑150 (MQ=255)

                                                                                                                                          cagccTGGCCAGCGGCCAGGTCTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACaa                                                                                      >  2:190433/4‑76 (MQ=25)

                                                                                                                                          cagccTGGCCAGCGGCCAGGTCTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtaggt            >  1:1949905/4‑150 (MQ=255)

                                                                                                                                          aGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTc           <  1:5027602/150‑1 (MQ=255)

                                                                                                                                           agccTGGCCAGCGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGCAACACTAATATTCCACCTCCAGGGCACAAGGACCTCGTCAGTTATGAGCAGGCTAGACGCGCCGGCGCATGTCGTAGGCTAGTTTTTCCAGCATCAGGTAGGTca          >  1:1636729/3‑150 (MQ=255)

                                                                                                                                            aCCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTcac         <  2:2062475/150‑1 (MQ=255)

                                                                                                                                              cTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTcacca       >  1:5257023/1‑150 (MQ=255)

                                                                                                                                               tGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                         >  1:210972/1‑131 (MQ=255)

                                                                                                                                                ggCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCATGATCAGGTAGGTCACCACt     <  2:3828976/150‑1 (MQ=255)

                                                                                                                                                  ccGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGc   <  1:1689652/150‑1 (MQ=255)

                                                                                                                                                  ccGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGc   <  1:1455172/150‑1 (MQ=255)

                                                                                                                                                    gCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGttttt                            >  1:823933/1‑123 (MQ=255)

GCATGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGCC  >  NC_002947/3699261‑3699557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`