BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,699,411	T→C	5.1%	S15G (AGC→GGC) ‡	PP_5595 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,699,411	0	T	C	5.1%	627.6 / 13.5	194	S15G (AGC→GGC) ‡	PP_5595	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (97/87); new base C (5/5); total (102/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 196 total aligned reads displayed.

CATGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGCCAAC  >  NC_002947/3699262‑3699560

cATGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCt                                                                                                                                                        <  1:656910/150‑1 (MQ=255)

 aTGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTg                                                                                                                                                       >  1:4922677/1‑150 (MQ=255)

 aTGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCGCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTg                                                                                                                                                       <  1:273988/150‑1 (MQ=255)

     ccTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCa                                                                                                                                                   <  2:4998787/150‑1 (MQ=255)

     ccTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCa                                                                                                                                                   <  2:4998726/150‑1 (MQ=255)

     ccTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCa                                                                                                                                                   <  1:3381315/150‑1 (MQ=255)

      cTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAg                                                                                                                                                  >  1:5236261/1‑150 (MQ=255)

          cTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTg                                                                                                                                             <  2:4922677/150‑1 (MQ=255)

          cTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTg                                                                                                                                             >  1:2539703/1‑150 (MQ=255)

            ttCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGc                                                                                                                                           <  2:955648/150‑1 (MQ=255)

               gTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAAc                                                                                                                                        >  2:4889162/1‑150 (MQ=255)

                   ctgcATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatc                                                                                                                                    <  1:4109159/150‑1 (MQ=255)

                   ctgcATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatc                                                                                                                                    <  1:2466386/150‑1 (MQ=255)

                        tGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatcatcat                                                                                                                               <  2:5378584/150‑1 (MQ=255)

                        tGACCAGTTGATCGCTTCGGCGGGTACGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACcatcatcat                                                                                                                               <  2:5274796/150‑1 (MQ=255)

                          aCCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCAtcatcatca                                                                                                                             >  2:1455172/1‑150 (MQ=255)

                               ttGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCa                                                                                                                        <  2:4582220/150‑1 (MQ=255)

                                  aTCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGa                                                                                                                     >  1:2062475/1‑150 (MQ=255)

                                   tCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGac                                                                                                                    >  2:4350378/1‑149 (MQ=255)

                                   tCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGaa                                                                                                                    >  1:5109286/1‑150 (MQ=255)

                                    cGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCatcatcatc                                                                                                                              <  1:1789074/139‑1 (MQ=255)

                                    cGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCatcatcatc                                                                                                                              >  2:1789074/1‑139 (MQ=255)

                                    cGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAac                                                                                                                   <  1:1427341/150‑1 (MQ=255)

                                       ttCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAg                                                                                                                       <  2:5216127/143‑1 (MQ=255)

                                           gcggGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCAtt                                                                                                            <  2:5122202/150‑1 (MQ=255)

                                            cggGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCAttt                                                                                                           <  2:3172028/150‑1 (MQ=255)

                                              ggTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTc                                                                                                         <  1:3185304/150‑1 (MQ=255)

                                                  tGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCAcc                                                                                                     <  1:4029392/150‑1 (MQ=255)

                                                     tGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTcc                                                                                                  >  1:3717361/1‑150 (MQ=255)

                                                        tgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                               >  1:1960970/1‑150 (MQ=255)

                                                        tgtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAgg                                                                                               <  1:3727121/150‑1 (MQ=255)

                                                         gtagctgtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAggg                                                                                              >  1:114605/1‑150 (MQ=255)

                                                               gtaACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAg                                                                                        <  2:4153724/150‑1 (MQ=255)

                                                                  aCAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGcc                                                                                     >  2:3059170/1‑150 (MQ=255)

                                                                   cAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGccc                                                                                    <  2:3462525/150‑1 (MQ=255)

                                                                    aGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCt                                                                                   >  1:930701/1‑150 (MQ=255)

                                                                      gtgtGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCACCCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCg                                                                                 >  1:5014744/1‑150 (MQ=255)

                                                                      gtgtGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCTTTTTCCACCTCCAGGGCACAAGGCCCTCg                                                                                 >  1:1485113/1‑150 (MQ=255)

                                                                      gtgtGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCg                                                                                 >  2:287402/1‑150 (MQ=255)

                                                                        gtgCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCACCCCTCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTc                                                                               >  1:1606760/1‑150 (MQ=255)

                                                                         tgCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCACCCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCa                                                                              >  1:2268233/1‑150 (MQ=255)

                                                                           tcGCTCCTCCAGCGACGCCGTGACGCCGGTGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCCCAAGGCCCTCGTCAgg                                                                            >  2:2152030/2‑150 (MQ=38)

                                                                           tcGCTCCTCCAGCGACGCCGTGACGCCGGTGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                            >  2:4283673/2‑150 (MQ=38)

                                                                           tcGCTCCTCCAGCGACGCCGTGACGCCGGTGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                            >  2:32170/2‑150 (MQ=38)

                                                                           ccGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                            >  2:2542498/1‑150 (MQ=255)

                                                                           ccGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAgg                                                                            >  1:3519266/1‑150 (MQ=255)

                                                                            cGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGt                                                                                >  2:4050859/1‑145 (MQ=255)

                                                                            cGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGt                                                                           >  1:5255235/1‑150 (MQ=255)

                                                                              cTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCt                                                                         <  1:5265928/150‑1 (MQ=255)

                                                                              cTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCt                                                                         >  1:4375244/1‑150 (MQ=255)

                                                                              cTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCt                                                                         >  2:3008634/1‑150 (MQ=255)

                                                                               tCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTg                                                                        <  1:4594549/150‑1 (MQ=255)

                                                                                ccACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGa                                                                       <  1:3094994/150‑1 (MQ=255)

                                                                                ccACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGa                                                                       >  1:67285/1‑150 (MQ=255)

                                                                                 cACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAg                                                                      >  1:3602785/1‑150 (MQ=255)

                                                                                    cGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAg                                                                   <  1:574923/150‑1 (MQ=255)

                                                                                      gCGGCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑c                                                                                                                                               >  1:698345/1‑72 (MQ=255)

                                                                                      gCGGCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑c                                                                                                                                               <  2:698345/72‑1 (MQ=255)

                                                                                         tCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTg                                                              >  2:1142488/1‑150 (MQ=255)

                                                                                          cACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTTGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGt                                                             <  1:4499884/150‑1 (MQ=255)

                                                                                            cGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCAGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGAAGGCTGGTcg                                                           >  2:5394487/1‑150 (MQ=255)

                                                                                                gTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCCGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGcc                                                       >  2:1682739/1‑150 (MQ=255)

                                                                                                         ggagCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATgg                                              >  1:951748/1‑150 (MQ=255)

                                                                                                             cGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                          <  1:310045/150‑1 (MQ=255)

                                                                                                             cGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                          >  2:469704/1‑150 (MQ=255)

                                                                                                             cGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCttt                                          <  2:4956464/150‑1 (MQ=255)

                                                                                                              gAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTg                                         <  1:1339776/150‑1 (MQ=255)

                                                                                                              gAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTg                                         <  1:3994408/150‑1 (MQ=255)

                                                                                                                gTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCCGGCTTGTCGCGCCGGCGCATGGCTTTGgc                                       >  2:4442687/1‑150 (MQ=255)

                                                                                                                gTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGgc                                       >  2:1002371/1‑150 (MQ=255)

                                                                                                                gTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGgc                                       <  2:3283413/150‑1 (MQ=255)

                                                                                                                 tCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGgcg                                      >  1:529184/1‑150 (MQ=255)

                                                                                                                   aGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGcgcg                                    <  1:3348409/150‑1 (MQ=255)

                                                                                                                     cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGtt                                  >  1:3041548/1‑150 (MQ=255)

                                                                                                                     cacaTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTGTGGCGCGtt                                  >  2:3507905/1‑150 (MQ=255)

                                                                                                                        aTTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTc                                                                                                   >  2:5249726/1‑82 (MQ=255)

                                                                                                                          tGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTcc                             >  1:5419579/1‑150 (MQ=255)

                                                                                                                          tGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTcc                             <  1:2899375/150‑1 (MQ=255)

                                                                                                                          tGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTcc                             >  1:2696121/1‑150 (MQ=255)

                                                                                                                             aaGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAgg                          >  2:1084775/1‑150 (MQ=255)

                                                                                                                               gCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGAt                        <  1:147595/150‑1 (MQ=255)

                                                                                                                               gCTTCGGCCCAGACCGGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGAt                        >  2:4552516/1‑150 (MQ=255)

                                                                                                                                cTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATc                       <  2:3140484/150‑1 (MQ=255)

                                                                                                                                cTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATc                       >  1:5130079/1‑150 (MQ=255)

                                                                                                                                    ggCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggt                   <  1:1129129/150‑1 (MQ=255)

                                                                                                                                      cccAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtag                 <  1:5136328/150‑1 (MQ=255)

                                                                                                                                      cccAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtag                 <  2:2805511/150‑1 (MQ=255)

                                                                                                                                       ccAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtagg                >  1:1024291/1‑150 (MQ=255)

                                                                                                                                        cAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtaggt               >  2:200357/1‑150 (MQ=255)

                                                                                                                                         cagccTGGCCAGCGGCCAGGTCTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCaggtaggt               >  1:1949905/4‑150 (MQ=255)

                                                                                                                                          gACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTca             <  2:1728799/150‑1 (MQ=255)

                                                                                                                                          agccTGGCCAGCGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGCAACACTAATATTCCACCTCCAGGGCACAAGGACCTCGTCAGTTATGAGCAGGCTAGACGCGCCGGCGCATGTCGTAGGCTAGTTTTTCCAGCATCAGGTAGGTca             >  1:1636729/3‑150 (MQ=255)

                                                                                                                                              tGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                            <  2:210972/131‑1 (MQ=255)

                                                                                                                                              tGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCa                            >  1:210972/1‑131 (MQ=255)

                                                                                                                                               ggCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCATGATCAGGTAGGTCACCACt        <  2:3828976/150‑1 (MQ=255)

                                                                                                                                                 ccGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGc      <  1:1455172/150‑1 (MQ=255)

                                                                                                                                                 ccGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGc      <  1:1689652/150‑1 (MQ=255)

                                                                                                                                                   gCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGttttt                               >  1:823933/1‑123 (MQ=255)

                                                                                                                                                   gCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGttttt                               <  2:823933/123‑1 (MQ=255)

                                                                                                                                                     tGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGCCAAc  >  1:2140873/1‑150 (MQ=255)

CATGTCCTTGCTTTCGTTGCTGCATGACCAGTTGATCGCTTCGGCGGGTATGTTGCTGTAGCTGTAACAGGTGTGCCGCTCCACCGGCGTCACGGCGTCGCTGGAGGAGCGAGTCAGCACATTGCAAGCTTCGGCCCAGACCTGGCCGCTGGCCAGG‑CTGGCAACCATCATCATCAAGGCAGGAACACGCATTTTCCACCTCCAGGGCACAAGGCCCTCGTCAGGTCTGAGCAGGCTTGTCGCGCCGGCGCATGGCTTTGGCGCGTTTTTCCAGGATCAGGTAGGTCACCACTGCCAAC  >  NC_002947/3699262‑3699560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`