BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,729,090	T→A	11.8%	L113Q (CTG→CAG)	PP_3292 →	membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,729,090	0	T	A	11.8%	431.2 / 37.9	155	L113Q (CTG→CAG)	PP_3292	membrane protein
Reads supporting (aligned to +/- strand): ref base T (59/76); new base A (7/11); total (68/87)
Fisher's exact test for biased strand distribution p-value = 8.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 159 total aligned reads displayed.

GGCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGGCGC  >  NC_002947/3728942‑3729239

    gggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCTCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                                  <  1:3820187/150‑1 (MQ=255)

    gggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                                  <  2:4858579/150‑1 (MQ=255)

    gggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTgg                                                                                                                                                  <  2:4858505/150‑1 (MQ=255)

     ggCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTggg                                                                                                                                                 <  2:414650/150‑1 (MQ=255)

       cGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGca                                                                                                                                               <  2:107740/150‑1 (MQ=255)

         gTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGTCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGcaca                                                                                                                                             <  2:1009558/150‑1 (MQ=255)

         gTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGcaca                                                                                                                                             >  2:1676624/1‑150 (MQ=255)

         gTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGcaca                                                                                                                                             <  1:2131948/150‑1 (MQ=255)

          tGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGccag                                                                                                                                             <  1:5318035/150‑4 (MQ=255)

          tGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCAGCAGGGccagg                                                                                                                                            <  2:246254/150‑5 (MQ=255)

           gccgCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAgg                                                                                                                                           >  2:342808/4‑150 (MQ=255)

           gCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGccagg                                                                                                                                            <  1:2377092/150‑5 (MQ=255)

           gCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAgg                                                                                                                                           <  1:5376329/150‑1 (MQ=255)

             aGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCa                                                                                                                                         >  1:2428453/1‑150 (MQ=255)

             aGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCa                                                                                                                                         >  2:3960725/1‑150 (MQ=255)

              gCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAc                                                                                                                                        <  2:69487/150‑1 (MQ=255)

              gCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAc                                                                                                                                        <  2:69500/150‑1 (MQ=255)

                  ggCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaaca                                                                                                                                    <  2:4869124/150‑5 (MQ=255)

                  ggCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTGTGCCCAGCAGGGC‑CAGGCAcaaca                                                                                                                                    <  2:4164075/150‑5 (MQ=255)

                    cGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtg                                                                                                                                  <  1:3598609/150‑1 (MQ=255)

                     gTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtgg                                                                                                                                 <  1:324179/150‑1 (MQ=255)

                     gTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtgg                                                                                                                                 <  2:1137040/150‑1 (MQ=255)

                       gAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggct                                                                                                                               <  1:5021909/150‑1 (MQ=255)

                        aaGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggctg                                                                                                                              <  1:680980/150‑1 (MQ=255)

                         aGCGCACCGTTGGCAAAGCCATTTTCAACCGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtggctgg                                                                                                                             <  2:3462829/150‑1 (MQ=255)

                          gcgcACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAgg                                                                                                                                           <  2:1261860/135‑1 (MQ=255)

                          gcgcACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAgg                                                                                                                                           >  1:1261860/1‑135 (MQ=255)

                           cgcACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCa                                                                                                                           <  1:2230867/150‑1 (MQ=255)

                           cgcACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCa                                                                                                                           <  2:93025/150‑1 (MQ=255)

                              aCCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATTGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAggg                                                                                                                        <  2:1455506/150‑1 (MQ=255)

                                cGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGgcg                                                                                                                      >  1:2881103/1‑150 (MQ=255)

                                       aaaGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTg                                                                                                               >  1:2634973/1‑150 (MQ=255)

                                         aGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGAc                                                                                                             <  2:4572362/150‑1 (MQ=255)

                                              ttttCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTtg                                                                                                                                  >  2:3818170/1‑124 (MQ=255)

                                               tttCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGAt                                                                                                       <  2:3910855/150‑1 (MQ=255)

                                                 tCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCg                                                                                                     >  2:631367/1‑150 (MQ=255)

                                                   aaCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGTGAATCGAACATCCTGCTGGTGGTTCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTg                                                                                                   <  1:1696340/150‑1 (MQ=255)

                                                   aaCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTg                                                                                                   >  2:2143407/1‑150 (MQ=255)

                                                    aCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTgg                                                                                                  <  1:956997/150‑1 (MQ=255)

                                                    aCGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTgg                                                                                                  <  1:5109632/150‑1 (MQ=255)

                                                     cGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTATTGGCTGGCAGGGCGCTTAGTGACGCAGATCGTGGc                                                                                                 <  2:3716722/150‑1 (MQ=255)

                                                         ggaacgccAGTCTGGCCTGCCAGCCTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAAc                                                                                             >  1:4097973/8‑150 (MQ=255)

                                                         cAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAAc                                                                                             >  1:2218357/1‑150 (MQ=255)

                                                          aGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACt                                                                                            >  2:1450879/1‑150 (MQ=255)

                                                             agaCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCg                                                                                         <  1:3691667/150‑1 (MQ=255)

                                                              gaCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCATGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                        <  1:37970/150‑1 (MQ=255)

                                                              gaCAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                        <  1:3857730/150‑1 (MQ=255)

                                                                 aGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCCGCTGGGCCCAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGc                                                                                     >  1:5201078/1‑150 (MQ=255)

                                                                     tGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAAc                                                                                 >  2:5233685/1‑150 (MQ=255)

                                                                                 cTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCaa                                                                     <  1:2101747/150‑1 (MQ=255)

                                                                                 cTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCaa                                                                     >  2:3105241/1‑150 (MQ=255)

                                                                                  tGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGTGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAg                                                                    <  1:330467/150‑1 (MQ=255)

                                                                                  tGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAg                                                                    <  2:2130249/150‑1 (MQ=255)

                                                                                   ggCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGt                                                                   <  1:2547633/150‑1 (MQ=255)

                                                                                    gCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTg                                                                  >  1:5198611/1‑150 (MQ=255)

                                                                                    gCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTg                                                                  <  1:1333330/150‑1 (MQ=255)

                                                                                     cGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTgg                                                                 <  1:1119440/150‑1 (MQ=255)

                                                                                      gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGa                                                                <  2:3154105/150‑1 (MQ=255)

                                                                                      gTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGa                                                                <  1:2143407/150‑1 (MQ=255)

                                                                                       ttCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACAGCGAGGCCAACCTGCAGGCTCAAGTGGat                                                               >  1:1410356/1‑150 (MQ=255)

                                                                                          cTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGAtata                                                            >  1:4379166/1‑150 (MQ=255)

                                                                                          cTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCGTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACAGCGAGGCCAACCTGCAGGCTCAAGTGGAtata                                                            >  1:1923585/1‑150 (MQ=255)

                                                                                             cGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAccc                                                         <  1:794382/150‑1 (MQ=255)

                                                                                                tCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGaa                                                      <  1:342808/150‑1 (MQ=255)

                                                                                                  aGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGa                                                    >  2:4040624/1‑150 (MQ=255)

                                                                                                       cTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaa                                               <  2:2103019/150‑1 (MQ=255)

                                                                                                       cTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaa                                               <  1:1807775/150‑1 (MQ=255)

                                                                                                       cTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCATTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaa                                               <  1:1046462/150‑1 (MQ=255)

                                                                                                        tCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                        <  2:1937808/108‑1 (MQ=255)

                                                                                                        tCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCCACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaaa                                              >  1:2382540/1‑150 (MQ=255)

                                                                                                        tCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCGTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGa                                                                                        >  1:1937808/1‑108 (MQ=255)

                                                                                                         cGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGaaaaa                                             <  1:5187591/150‑1 (MQ=255)

                                                                                                               tCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAACAACTCAcc                                       >  1:4811235/1‑150 (MQ=255)

                                                                                                                 gAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACcgc                                     >  2:3355628/1‑150 (MQ=255)

                                                                                                                  aaCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCCACCTGCCGGCTCAAGTGGATATACCCGAAGATGAAAAAATCACcgcg                                    >  1:4250771/1‑150 (MQ=255)

                                                                                                                  aaCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATAGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATAACCGAAGATGAAAAACTCACcgcg                                    >  1:4250565/1‑150 (MQ=255)

                                                                                                                   aCATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCgcgc                                   <  1:86639/150‑1 (MQ=255)

                                                                                                                          gctgGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCCACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCgg                            >  1:4159457/1‑150 (MQ=255)

                                                                                                                          gctgGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCgg                            <  2:130702/150‑1 (MQ=255)

                                                                                                                           ctgGTGGTGCTGTTGTGCCTG‑GCCCAGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGata                                                              >  1:591797/1‑115 (MQ=255)

                                                                                                                               tggtgCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAAc                       <  1:464903/150‑1 (MQ=255)

                                                                                                                                  tgCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCTGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCTAGTGGATATACCCGAAGTTGAAAAACTCACCGCGCTTCTCGGCAAACCgg                    >  2:3627078/1‑150 (MQ=255)

                                                                                                                                  tgCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCgg                    <  2:3751543/150‑1 (MQ=255)

                                                                                                                                   gCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCTAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgt                   >  2:4087750/1‑150 (MQ=255)

                                                                                                                                   gCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgt                   >  2:571726/1‑150 (MQ=255)

                                                                                                                                     tgttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGgtgt                 >  1:1534787/1‑150 (MQ=255)

                                                                                                                                       ttgtGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGc               >  2:2815843/1‑150 (MQ=255)

                                                                                                                                         gtgCCTGTGCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCAc             <  2:501630/150‑1 (MQ=255)

                                                                                                                                          tgCCTGTGCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACg            >  1:2410738/1‑150 (MQ=255)

                                                                                                                                          tgCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACg            >  1:1621604/1‑150 (MQ=255)

                                                                                                                                          tgCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACg            >  1:3218583/1‑150 (MQ=255)

                                                                                                                                            cctgtgCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCAc             >  2:684936/6‑147 (MQ=255)

                                                                                                                                            cctgtgCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTg          >  2:580974/6‑150 (MQ=255)

                                                                                                                                            cctgtgCCCAGCAGGGC‑CAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTg          >  2:2827190/6‑150 (MQ=255)

                                                                                                                                            ccTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTg          >  1:4217684/1‑150 (MQ=255)

                                                                                                                                             cTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGc         >  2:2435085/1‑150 (MQ=255)

                                                                                                                                                 gCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTg      >  2:651929/1‑150 (MQ=255)

                                                                                                                                                   ccTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGgc    >  2:2762046/1‑150 (MQ=255)

                                                                                                                                                   ccTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGgc    >  2:5333247/1‑150 (MQ=255)

                                                                                                                                                     tgctgGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGgcgc  >  1:2354254/1‑150 (MQ=255)

GGCTGGGCGGTGCAGCGGGGCGTGAAGCGCACCGTTGGCAAAGCCATTTTCAACGGCCAGGAGACAGGCTGGCAGGCCAGACTGGCGTTCCTTCGCTCAGTGGCTCGGGAATCGAACATCCTGCTGGTGGTGCTGTTGTGCCTG‑GCCCTGCTGGGCACAGGCACCTTTTGGCTGGCAGGGCGCTCAGTGACGCAGATCGTGGCGAACTGCGAGGCCAACCTGCAGGCTCAAGTGGATATACCCGAAGATGAAAAACTCACCGCGCTTCTCGGCAAACCGGTGTGCACGTGCCTGGCGC  >  NC_002947/3728942‑3729239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`