Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,764,907 A→T 9.8% N62I (AAC→ATC)  PP_3326 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,764,9070AT 9.8% 437.8 / 30.0 150N62I (AAC→ATC) PP_3326hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (60/75);  new base T (2/13);  total (62/88)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01

Only 100 of 154 total aligned reads displayed.

CCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTC  >  NC_002947/3764758‑3765055
                                                                                                                                                      |                                                                                                                                                    
ccTGATGTTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGaa                                                                                                                                                      >  2:3522140/1‑150 (MQ=255)
ccTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGaa                                                                                                                                                      <  2:5312049/150‑1 (MQ=255)
  tGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGATCa                                                                                                                                                    <  1:1558655/150‑1 (MQ=255)
  tGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACa                                                                                                                                                    <  1:5422572/150‑1 (MQ=255)
   gATGGTCAGCGTCAACGCAGGCGCCCATGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACAt                                                                                                                                                   >  1:814636/1‑150 (MQ=255)
   gATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGATAAGATCAAGCGCGCC‑AGGCGCTCGACCGTGACCGGGATCAt                                                                                                                                                   <  1:2025992/150‑1 (MQ=255)
    aTGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGATCATg                                                                                                                                                  <  2:3748503/150‑1 (MQ=255)
    aTGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGATCATg                                                                                                                                                  <  1:3656810/150‑1 (MQ=255)
     tGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGa                                                                                                                                                 <  1:1417203/150‑1 (MQ=255)
       gTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATc                                                                                                                                               <  2:2869723/150‑1 (MQ=255)
         cAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATccc                                                                                                                                             <  2:851671/150‑1 (MQ=255)
           gCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCgg                                                                                                                                           >  2:780756/1‑150 (MQ=255)
            cGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGt                                                                                                                                          <  1:3731332/150‑1 (MQ=255)
            cGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCC‑AGGCGCTCGACCGTGACCGGGATCATGTTCCCGGt                                                                                                                                          <  1:3768162/150‑1 (MQ=38)
             gTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGATCATGTTCCCGGTc                                                                                                                                         <  1:734680/150‑1 (MQ=255)
               cAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCAc                                                                                                                                       <  2:2261221/150‑1 (MQ=255)
                aaCGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACg                                                                                                                                      <  1:4189510/150‑1 (MQ=255)
                   gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑TGTGACCGGGATCATGTTCCCGGTCACgtcg                                                                                                                                   <  2:4529005/150‑4 (MQ=255)
                   gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTc                                                                                                                                   <  1:4456328/150‑1 (MQ=255)
                   gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTc                                                                                                                                   <  2:3220724/150‑1 (MQ=255)
                   gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTc                                                                                                                                   <  2:3366175/150‑1 (MQ=255)
                   gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTc                                                                                                                                   <  1:3295036/150‑1 (MQ=255)
                   gCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTc                                                                                                                                   <  1:2826701/150‑1 (MQ=255)
                       gcgcCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAgc                                                                                                                               >  1:1371837/1‑150 (MQ=255)
                        cgcCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAgcg                                                                                                                              >  2:5179041/1‑150 (MQ=255)
                           ccACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCt                                                                                                                           >  2:1712269/1‑150 (MQ=255)
                            cACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTg                                                                                                                          >  2:1170299/1‑150 (MQ=255)
                              cGGCCTGTGGACCGAGGAGCGACGTGGAAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCACCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGc                                                                                                                        <  1:6321/150‑1 (MQ=255)
                                 ccTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGa                                                                                                                     <  1:245755/150‑1 (MQ=255)
                                 ccTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGa                                                                                                                     <  1:1175334/150‑1 (MQ=255)
                                         ccGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCg                                                                                                             >  1:774923/1‑150 (MQ=255)
                                           gaggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                                           <  1:2428130/150‑1 (MQ=255)
                                           gaggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtg                                                                                                           <  2:4483888/150‑1 (MQ=255)
                                             ggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                                                                                                         >  1:2245918/1‑150 (MQ=255)
                                             ggagCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgct                                                                                                         >  1:4545065/1‑150 (MQ=255)
                                               agCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGtgctgc                                                                                                       <  2:1639355/150‑1 (MQ=255)
                                                gCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTACGA‑CGTGACCGGGAACATGATCCCGGACACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCa                                                                                                      >  2:1226302/1‑150 (MQ=255)
                                                     gTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGATCAt                                                                                                                                                   >  2:851010/1‑100 (MQ=255)
                                                       ggCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACtt                                                                                               >  2:193382/1‑150 (MQ=255)
                                                         cAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAc                                                                                             <  1:625493/150‑1 (MQ=255)
                                                          aaTATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                            >  1:2629408/1‑150 (MQ=255)
                                                          aaTATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcc                                                                                            >  1:2629293/1‑150 (MQ=255)
                                                            tctCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTAcccc                                                                                          <  2:4573139/148‑1 (MQ=255)
                                                             gccgtggCCGTACACGGCCTCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGACCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCg                                                                                         >  1:4064247/6‑150 (MQ=255)
                                                              tCGAGGCCTTGTACGGCCACGGCTCCGAGGATAACGCCTACAAGGCGGAAAAGATCATGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACTTGATCCCGGTCTCGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGc                                                                                        <  1:1226302/150‑1 (MQ=255)
                                                              tCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGc                                                                                        >  1:3503396/1‑150 (MQ=255)
                                                                  ggCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGc                                                                                    <  1:1901025/150‑1 (MQ=255)
                                                                   gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                                   <  2:4317404/150‑1 (MQ=255)
                                                                   gCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCg                                                                                   <  1:1619395/150‑1 (MQ=255)
                                                                           cGGCCACGGCGCCGAGGATAACGCCTACCAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTGACCCCGCCTGCGGCACTTGc                                                                           <  2:5251090/150‑1 (MQ=255)
                                                                            ggCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGcc                                                                          <  1:643687/150‑1 (MQ=255)
                                                                                aCGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgc                                                                      <  1:535505/150‑1 (MQ=255)
                                                                                  ggCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGtgctg                                                                    >  1:4467279/1‑150 (MQ=255)
                                                                                    cgcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTgctgct                                                                  <  1:3179973/150‑1 (MQ=255)
                                                                                     gcCGAGGATAACGCCTACTAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGTGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                                 <  1:3554701/150‑1 (MQ=255)
                                                                                     gcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                                 <  2:4672754/150‑1 (MQ=255)
                                                                                     gcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                                 <  2:1367400/150‑1 (MQ=255)
                                                                                     gcCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGctgctg                                                                 <  1:1827959/150‑1 (MQ=255)
                                                                                      ccGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTgg                                                                >  1:2231102/1‑150 (MQ=255)
                                                                                            aTAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCAGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACg                                                          <  2:3066131/150‑1 (MQ=255)
                                                                                                cGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTGACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGcc                                                      <  2:5107409/150‑1 (MQ=255)
                                                                                                  ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                    >  2:3671344/1‑150 (MQ=255)
                                                                                                  ccTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                    >  2:611350/1‑150 (MQ=255)
                                                                                                   cTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTg                                                   <  1:3586503/150‑1 (MQ=255)
                                                                                                    tACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                    >  2:2045269/1‑148 (MQ=255)
                                                                                                    tACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCtt                                                    <  1:2045269/148‑1 (MQ=255)
                                                                                                    tACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATACCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCCCTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTgg                                                  >  1:3683172/1‑150 (MQ=255)
                                                                                                      cAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTc                                                <  1:4881903/150‑1 (MQ=255)
                                                                                                       aaGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTca                                               <  1:4401463/150‑1 (MQ=255)
                                                                                                         ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                             <  1:4905837/150‑1 (MQ=255)
                                                                                                         ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                             <  1:43380/150‑1 (MQ=255)
                                                                                                         ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                             <  2:3717960/150‑1 (MQ=255)
                                                                                                         ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                             <  1:2969829/150‑1 (MQ=255)
                                                                                                         ggCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTcaca                                             <  2:1078481/150‑1 (MQ=255)
                                                                                                          gCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAg                                            <  2:3834562/150‑1 (MQ=255)
                                                                                                            ggAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAgcg                                          >  2:96260/1‑150 (MQ=255)
                                                                                                               aaaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGccc                                       <  2:3503396/150‑1 (MQ=255)
                                                                                                               aaaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGccc                                       <  2:4467279/150‑1 (MQ=255)
                                                                                                                aaGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACGTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCg                                      <  2:5278086/150‑1 (MQ=255)
                                                                                                                        gcgcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACCAGCa                              >  1:4971739/1‑150 (MQ=255)
                                                                                                                          gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTGGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAAt                            <  2:1801079/150‑1 (MQ=255)
                                                                                                                          gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAAt                            <  2:1226307/150‑1 (MQ=255)
                                                                                                                          gcgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAAt                            <  1:841162/150‑1 (MQ=255)
                                                                                                                           cgcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATg                           <  2:4088995/150‑1 (MQ=255)
                                                                                                                            gcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATgg                          <  2:1371837/150‑1 (MQ=255)
                                                                                                                            gcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATg                           <  1:1243105/149‑1 (MQ=255)
                                                                                                                            gcCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATg                           >  2:1243105/1‑149 (MQ=255)
                                                                                                                               tGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTc                       >  2:4380946/1‑150 (MQ=255)
                                                                                                                               tGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTc                       >  2:4380949/1‑150 (MQ=255)
                                                                                                                                gggCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCa                      >  2:5151118/1‑150 (MQ=255)
                                                                                                                                      tcgaccGTGACCGGGATCATGTTCCCGGTCAC‑GTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAAt                    >  2:1260190/6‑146 (MQ=39)
                                                                                                                                      tcgaccGTGACCGGGATCATGTTCCCGGTCAC‑GTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAAt                    <  1:1260190/141‑1 (MQ=39)
                                                                                                                                        gA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGAt              >  1:4836920/1‑150 (MQ=255)
                                                                                                                                         a‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATc             >  2:2422044/1‑150 (MQ=255)
                                                                                                                                         a‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATc             <  1:193382/150‑1 (MQ=255)
                                                                                                                                             tGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCa          >  2:3779308/1‑150 (MQ=255)
                                                                                                                                              gACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAc         >  1:1055360/1‑150 (MQ=255)
                                                                                                                                              gACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCAc         >  1:1055038/1‑150 (MQ=255)
                                                                                                                                                     aaCATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTc  >  1:3893989/1‑150 (MQ=255)
                                                                                                                                                     aaCATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTc  >  1:3894000/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CCTGATGGTCAGCGTCAACGCAGGCGCCCACGGCCTGTGGACCGAGGAGCGACGTGGCAATATCGAGGCCGTGTACGGCCACGGCGCCGAGGATAACGCCTACAAGGCGGAAAAGATCAAGCGCGCCTGGGCGTTCGA‑CGTGACCGGGAACATGATCCCGGTCACGGTCGAGCGCCTGGCGGATCATGCCGTGCTGCAACCACTTACCCCGCCTGCGGCACTTGCCGTGCTGCTGGACAACGGGCCTTGGTCACAGCGCCCGGACAAGCAATGGGTCAATCAGGATCGCACCCATGTC  >  NC_002947/3764758‑3765055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑