BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,770,225	T→G	5.6%	L92R (CTG→CGG)	PP_5600 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,770,225	0	T	G	5.6%	575.6 / 16.9	178	L92R (CTG→CGG)	PP_5600	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (100/68); new base G (4/6); total (104/74)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 187 total aligned reads displayed.

TGATGCCGCCTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGC  >  NC_002947/3770076‑3770374

tgatgCCGCCTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCt                                                                                                                                                           >  1:1527357/1‑150 (MQ=255)

   tgCCGCCTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCt                                                                                                                                                        >  2:1747327/1‑150 (MQ=255)

    gccgccTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTc                                                                                                                                                       >  1:4879346/1‑150 (MQ=255)

       gccTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGcc                                                                                                                                                    >  1:2498994/1‑150 (MQ=255)

        ccTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCg                                                                                                                                                   <  1:1057149/150‑1 (MQ=255)

        ccTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGGCTCGCCg                                                                                                                                                   <  1:3596146/150‑1 (MQ=255)

            gTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGa                                                                                                                                               >  1:1446637/1‑150 (MQ=255)

              gCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGc                                                                                                                                             >  2:5052127/1‑150 (MQ=255)

               cACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGcc                                                                                                                                            >  2:54223/1‑150 (MQ=255)

               cACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTGTCGGCCTGCTGACCCTGCTCGCCGGCGAGcc                                                                                                                                            >  2:54416/1‑150 (MQ=255)

                 cGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCgg                                                                                                                                          <  1:2828516/150‑1 (MQ=255)

                 cGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCAgg                                                                                                                                          <  2:3351433/150‑1 (MQ=255)

                 cGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGGCTCGCCGGCGAGCAgg                                                                                                                                          <  2:4364706/150‑1 (MQ=255)

                  ggCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCggg                                                                                                                                         <  2:2649005/150‑1 (MQ=255)

                  ggCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGGCTCGCCGGCGAGCAggg                                                                                                                                         <  2:5072204/150‑1 (MQ=255)

                      gACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGc                                                                                                                                    <  2:4779348/150‑1 (MQ=255)

                      gACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGc                                                                                                                                    <  1:2182617/150‑1 (MQ=255)

                            agcGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTa                                                                                                                           >  2:3037075/1‑150 (MQ=255)

                             gcGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTAc                                                                                                                          <  2:3475738/150‑1 (MQ=255)

                                      tCCATGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCcaaccaac                                                                                                                 <  1:1616940/150‑1 (MQ=255)

                                      tCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCcaaccaac                                                                                                                 <  1:1004256/150‑1 (MQ=255)

                                            gTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGc                                                                                                           <  1:4917988/150‑1 (MQ=255)

                                                gCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACg                                                                                                       >  2:3749583/1‑150 (MQ=255)

                                                 cAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTTCCCAACCAACCTCCGCCACGc                                                                                                      >  1:3941619/1‑150 (MQ=255)

                                                  aaGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGcc                                                                                                     >  2:2315236/1‑150 (MQ=255)

                                                   aGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTATTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCt                                                                                                    <  1:3281898/150‑1 (MQ=255)

                                                    gACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTg                                                                                                   >  2:2184036/1‑150 (MQ=255)

                                                    gACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCGGCTCGCCGGCGAGCAgggtc                                                                                                                                       <  1:3032395/118‑3 (MQ=255)

                                                      cTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGcc                                                                                                 >  1:4282260/1‑150 (MQ=255)

                                                       ttCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGccc                                                                                                >  2:1641078/1‑150 (MQ=255)

                                                       ttCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGccc                                                                                                >  2:3437385/1‑150 (MQ=255)

                                                       ttCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCGCCGCCACCCCGGccc                                                                                                >  1:621027/1‑150 (MQ=255)

                                                        tCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGc                                                                                                                                                     <  2:106017/100‑1 (MQ=255)

                                                         cAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTATTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCaa                                                                                              <  1:1850817/150‑1 (MQ=255)

                                                          cgCGAGCCGCCCCTGTCTGCGGGGGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGGTGGCCGTCTTTGGCGGCCTGCTGACCCTGCTCGCGGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAag                                                                                             <  1:1066198/149‑1 (MQ=255)

                                                          aGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAag                                                                                             <  1:3885549/150‑1 (MQ=255)

                                                          aGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAag                                                                                             >  2:5142197/1‑150 (MQ=255)

                                                           gCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagc                                                                                            >  2:451509/1‑150 (MQ=255)

                                                            cGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagca                                                                                           <  1:5393255/150‑1 (MQ=255)

                                                              aTCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAagcagc                                                                                         <  1:3539205/150‑1 (MQ=255)

                                                               tCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAgcg                                                                                        >  1:2773576/1‑150 (MQ=255)

                                                                 cGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCt                                                                                      >  2:461128/1‑150 (MQ=255)

                                                                 cGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCGGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCt                                                                                      <  1:1342985/150‑1 (MQ=255)

                                                                  gACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CCGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTg                                                                                     >  1:3710818/1‑150 (MQ=255)

                                                                    cccTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGc                                                                                   <  2:4949053/150‑1 (MQ=255)

                                                                     ccTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCGTACccaa                                                                                                                      >  1:178871/1‑114 (MQ=255)

                                                                      cTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCa                                                                                 >  2:1502796/1‑150 (MQ=255)

                                                                       tGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCAc                                                                                <  2:3721475/150‑1 (MQ=255)

                                                                         tCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGcc                                                                                                 <  1:4225381/131‑1 (MQ=255)

                                                                         tCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGcc                                                                                                 >  2:4225381/1‑131 (MQ=255)

                                                                             ccGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCa                                                                          >  2:3822077/1‑150 (MQ=255)

                                                                             ccGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGACCCGGG‑GAGC‑‑‑CCCGTACCCAACAAACCTCCGCCACGCGTGCCCACGCAGCGCTGGCCACGGGTCa                                                                          >  1:2065750/1‑150 (MQ=255)

                                                                                     gACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTC‑TTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcgcg                                                                 >  1:1565863/1‑150 (MQ=255)

                                                                                          cGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTTCCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCctc                                                             <  2:3941619/150‑1 (MQ=255)

                                                                                          cGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCctc                                                             >  2:2045574/1‑150 (MQ=255)

                                                                                           gTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCctct                                                            >  1:3546332/1‑150 (MQ=255)

                                                                                             gATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTcc                                                          >  2:1581089/1‑150 (MQ=255)

                                                                                             gATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTcc                                                          >  2:4122461/1‑150 (MQ=255)

                                                                                              aTCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTccc                                                         >  2:752616/1‑150 (MQ=255)

                                                                                               tCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACAGGTCAATCCCCGCGCCTCTCCcg                                                        >  1:5178057/1‑150 (MQ=255)

                                                                                               tCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCACCGCCACGCCAGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcg                                                        >  1:807440/1‑150 (MQ=255)

                                                                                                 gCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgct                                                      >  2:2016184/1‑150 (MQ=255)

                                                                                                  ccAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgctt                                                     >  1:4287618/1‑150 (MQ=255)

                                                                                                  ccAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgctt                                                     >  2:1599284/1‑150 (MQ=255)

                                                                                                   cAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttc                                                    <  2:4586874/150‑1 (MQ=255)

                                                                                                    aGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcg                                                   >  1:2672583/1‑150 (MQ=255)

                                                                                                     gCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcgc                                                  <  1:2264972/150‑1 (MQ=255)

                                                                                                      ccTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcgcg                                                 >  1:3562791/1‑149 (MQ=255)

                                                                                                       cTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCcgcttcgctt                                                >  1:1630782/1‑150 (MQ=255)

                                                                                                          ttCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTtctc                                             <  2:5106246/150‑1 (MQ=255)

                                                                                                          ttCAGCGCCACACTGCTGGCCGTATTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTtctc                                             <  1:3401716/150‑1 (MQ=255)

                                                                                                           tCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCACCCAACCTCCGCCACGCCTGCCCCAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTcg                                            >  2:1707761/1‑150 (MQ=255)

                                                                                                             aGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTCCCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTcgcg                                          >  2:653510/1‑150 (MQ=255)

                                                                                                             aGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCtctc                                                           >  1:3944381/1‑133 (MQ=255)

                                                                                                              gcgcCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCCATCCCCGCGCCTCTCCCGCTTCGCGTCTCGCGt                                         >  2:3628350/1‑150 (MQ=255)

                                                                                                                gcCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcgc                                                                  <  2:3863569/123‑1 (MQ=255)

                                                                                                                 ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCACGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCGTCTCGCGTTCt                                      >  2:2472920/1‑150 (MQ=255)

                                                                                                                 ccACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCt                                      >  2:2472894/1‑150 (MQ=255)

                                                                                                                  cacaCTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTg                                     <  2:1446637/150‑1 (MQ=255)

                                                                                                                  cacaCTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTg                                     >  1:3846917/1‑150 (MQ=255)

                                                                                                                          tgGCCGTCTTTTTCGGCCTGCTTACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGcc                             >  1:4077741/1‑150 (MQ=255)

                                                                                                                          tgGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGcc                             >  1:4272784/1‑150 (MQ=255)

                                                                                                                            gCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCAc                           >  2:3247560/1‑150 (MQ=255)

                                                                                                                            gCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCAc                           >  2:1721413/1‑150 (MQ=255)

                                                                                                                            gCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCAc                           <  2:4879346/150‑1 (MQ=255)

                                                                                                                                 cTTTATCGGCCTGCTGACACTGCACGCCGGCGAGCCGGT‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTCGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTACTCGCGTTCTGACATCGCCACGAccc                      >  2:5332740/1‑150 (MQ=255)

                                                                                                                                     ttCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTg                  >  2:2964863/1‑150 (MQ=255)

                                                                                                                                      tCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTgc                 >  2:1847874/1‑150 (MQ=255)

                                                                                                                                          ccTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCGTCTCGCGTTCTGACATCGCCACGTCCCCGTgccgcc             >  2:2922133/1‑150 (MQ=255)

                                                                                                                                              gctgcccTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGag         >  1:4582315/5‑149 (MQ=255)

                                                                                                                                              gctgcccTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGAt         >  1:4582223/5‑150 (MQ=255)

                                                                                                                                              ctgACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCACCCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGAt         <  2:2498994/150‑1 (MQ=255)

                                                                                                                                               tgACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATc        <  2:1183899/150‑1 (MQ=255)

                                                                                                                                               ngACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGGTCTGACATCGCCACGTCCCCGTGCCGCCTGATc        >  1:2554881/2‑150 (MQ=255)

                                                                                                                                                 gcccGGCTCGCCGGCGAGCAGGGTCAGCAGGCCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGa          >  2:329818/2‑150 (MQ=255)

                                                                                                                                                 cccgtGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCGTACCCAACCAACCTCCGCCACGCCTGCCCACGCAGCGCTGGCCACTGGTCACTCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCgg      <  1:4555514/146‑1 (MQ=255)

                                                                                                                                                    cTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCa    >  1:172409/1‑149 (MQ=255)

                                                                                                                                                     tGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcgcg                                                                 <  2:4284559/87‑1 (MQ=255)

                                                                                                                                                     tGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCcgcg                                                                 <  2:498308/87‑1 (MQ=255)

                                                                                                                                                     tGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGc  >  2:5202164/1‑150 (MQ=255)

TGATGCCGCCTGGTGCACGGCCGACAGCAGCGGCATGGTCCAAGGTGAGCAAGACTTCAGCGATCCGACCCTGTCTGCCGGCGGTGACTGCGTGATCGCCAGCCTGTTCAGCGCCACACTGCTGGCCGTCTTTTTCGGCCTGCTGACCCTGCTCGCCGGCGAGCCGGG‑CAGC‑‑‑CCCTTACCCAACCAACCTCCGCCACGCCTGCCCAAGCAGCGCTGGCCACTGGTCAATCCCCGCGCCTCTCCCGCTTCGCTTCTCGCGTTCTGACATCGCCACGTCCCCGTGCCGCCTGATCGGCAGC  >  NC_002947/3770076‑3770374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`