Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,818,474 A→T 5.3% S104S (TCT→TCA)  cpxR ← two‑component system regulator CpxR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,818,4740AT 5.3% 643.1 / 17.0 188S104S (TCT→TCA) cpxRtwo‑component system regulator CpxR
Reads supporting (aligned to +/- strand):  ref base A (77/101);  new base T (6/4);  total (83/105)
Fisher's exact test for biased strand distribution p-value = 3.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 198 total aligned reads displayed.

GCGCCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGAGATGCCTGGC  >  NC_002947/3818325‑3818621
                                                                                                                                                     |                                                                                                                                                   
gcgcCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGga                                                                                                                                                     <  1:5122149/150‑1 (MQ=255)
gcgcCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGga                                                                                                                                                     <  1:1607515/150‑1 (MQ=255)
  gcCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGgaga                                                                                                                                                   <  1:107334/150‑1 (MQ=255)
   ccccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGagag                                                                                                                                                  >  1:404191/1‑150 (MQ=255)
   ccccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGagag                                                                                                                                                  >  2:4787859/1‑150 (MQ=255)
    cccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGc                                                                                                                                                 >  2:5187848/1‑150 (MQ=255)
    cccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGc                                                                                                                                                 >  2:2938052/1‑150 (MQ=255)
    cccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGc                                                                                                                                                 >  1:2008170/1‑150 (MQ=255)
    cccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCAGCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGc                                                                                                                                                 >  2:2938009/1‑150 (MQ=255)
    cccAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCGGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGc                                                                                                                                                 >  2:5324525/1‑150 (MQ=255)
      cAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTg                                                                                                                                               <  2:3483738/150‑1 (MQ=255)
      cAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTg                                                                                                                                               <  2:3483868/150‑1 (MQ=255)
           cgcCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGC‑‑CCAGCTCTCCCGGTGAGCTggtggcac                                                                                                                                        <  2:4571269/150‑7 (MQ=255)
             ccTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTgg                                                                                                                                        <  1:2022256/150‑1 (MQ=255)
              cTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTggg                                                                                                                                       <  1:4663075/150‑1 (MQ=255)
               tCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGc                                                                                                                                      <  1:2144677/150‑1 (MQ=255)
                    ccgccCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATa                                                                                                                                 <  2:3574735/150‑1 (MQ=255)
                       ccccGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTc                                                                                                                              <  1:1354749/150‑1 (MQ=255)
                       ccccGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTc                                                                                                                              <  1:3456561/150‑1 (MQ=255)
                        cccGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCa                                                                                                                             >  1:2704211/1‑150 (MQ=255)
                         ccGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCAt                                                                                                                            >  2:5394620/1‑150 (MQ=255)
                         ccGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCAt                                                                                                                            <  2:624440/150‑1 (MQ=255)
                          cGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATc                                                                                                                           <  2:2006670/150‑1 (MQ=255)
                           ggCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCg                                                                                                                          <  2:5115762/150‑1 (MQ=255)
                               acaTTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGccc                                                                                                                      <  2:1532414/150‑1 (MQ=255)
                                caTTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGcccc                                                                                                                     <  1:5334839/150‑1 (MQ=255)
                                 aTTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTAACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGccccc                                                                                                                    >  2:1287194/1‑150 (MQ=255)
                                  ttttCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCa                                                                                                                   >  2:3681061/1‑150 (MQ=255)
                                   tttCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAg                                                                                                                  >  1:1138487/1‑150 (MQ=255)
                                   tttCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAg                                                                                                                  >  2:5172547/1‑150 (MQ=255)
                                    ttCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGc                                                                                                                 >  2:4917728/1‑150 (MQ=255)
                                              cACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCgg                                                                                                       >  2:1697208/1‑150 (MQ=255)
                                              cACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCgg                                                                                                       >  1:2306960/1‑150 (MQ=255)
                                                cTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCa                                                                                                     >  2:3290502/1‑150 (MQ=255)
                                                   cGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATg                                                                                                  <  1:500957/150‑1 (MQ=255)
                                                       ccTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGt                                                                                              <  2:2274992/150‑1 (MQ=255)
                                                           ttCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGAt                                                                                          <  2:256120/150‑1 (MQ=255)
                                                              aGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGAtgtt                                                                                       <  2:2868420/150‑1 (MQ=255)
                                                              aGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGAtgtt                                                                                       <  2:2185220/150‑1 (MQ=255)
                                                              aGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTAACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGAtgtt                                                                                       <  1:1287194/150‑1 (MQ=255)
                                                                gTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGAtgttgt                                                                                     >  1:1001134/1‑150 (MQ=255)
                                                                    cTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGGTGTCAcc                                                                                 >  2:4051945/1‑150 (MQ=255)
                                                                     tCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACcg                                                                                >  2:3828554/1‑150 (MQ=255)
                                                                      cGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACcgc                                                                               <  2:1443225/150‑1 (MQ=255)
                                                                        ggCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGt                                                                             <  1:895510/150‑1 (MQ=255)
                                                                           gcgcTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCt                                                                          >  1:2425965/1‑150 (MQ=255)
                                                                           gcgcTGGTTTCACCCATCAGGTATGGCTGAACGCGACGCAGTATTGCCAGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTAGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGATGTCACCGCGTGCt                                                                          >  1:3922891/1‑150 (MQ=255)
                                                                             gcTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGt                                                                        >  2:2897870/1‑150 (MQ=255)
                                                                             gcTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGt                                                                        >  1:2171158/1‑150 (MQ=255)
                                                                             gcTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGt                                                                        >  2:4985008/1‑150 (MQ=255)
                                                                             gcTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGt                                                                        >  1:5277411/1‑150 (MQ=255)
                                                                               tGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTca                                                                      <  2:2054274/150‑1 (MQ=255)
                                                                                 gTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTc                                                                                                                              >  2:2491901/1‑92 (MQ=255)
                                                                                   ttCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTcagcag                                                                  >  1:1863270/1‑150 (MQ=255)
                                                                                   ttCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTcagcag                                                                  >  1:4270914/1‑150 (MQ=255)
                                                                                   ttCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTcagcag                                                                  <  1:4984539/150‑1 (MQ=255)
                                                                                     cACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTcagcagca                                                                <  2:3791875/150‑1 (MQ=255)
                                                                                       cccATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCt                                                                          <  2:4946625/138‑1 (MQ=255)
                                                                                       cccATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCt                                                                          >  1:4946625/1‑138 (MQ=255)
                                                                                        caaTCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAgcagcagca                                                             >  1:3762245/3‑150 (MQ=255)
                                                                                          aTCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCAcc                                                           <  2:4859794/150‑1 (MQ=255)
                                                                                          aTCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCAcc                                                           <  1:5172547/150‑1 (MQ=255)
                                                                                          aTCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCAcc                                                           <  2:1743586/150‑1 (MQ=255)
                                                                                           tCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCg                                                          >  1:3496511/1‑150 (MQ=255)
                                                                                             aGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGc                                                        >  2:3135400/1‑150 (MQ=255)
                                                                                                 aTGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCt                                                    <  1:5068486/150‑1 (MQ=255)
                                                                                                      tGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTa                                               >  1:4360013/1‑150 (MQ=255)
                                                                                                       gCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTAc                                              >  2:4725367/1‑150 (MQ=255)
                                                                                                         aCGCGACGCAGTATTGCCCGCAGGCGTGCCACCAACTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGt                                            <  1:3965992/150‑1 (MQ=255)
                                                                                                          cgcgACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACGTAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTg                                           <  2:4969452/150‑1 (MQ=255)
                                                                                                           gcgACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTgc                                          >  1:411333/1‑150 (MQ=255)
                                                                                                             gACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTgcac                                        <  1:4610313/150‑1 (MQ=255)
                                                                                                             gACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTgcac                                        <  2:4270914/150‑1 (MQ=255)
                                                                                                             gACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTgcac                                        <  2:1863270/150‑1 (MQ=255)
                                                                                                                  aGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGcacgcacg                                   >  2:2398648/1‑150 (MQ=255)
                                                                                                                    tATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCAcgcg                                 <  1:4035268/150‑1 (MQ=255)
                                                                                                                      ttGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACgcggc                               >  1:2890626/1‑150 (MQ=255)
                                                                                                                      ttGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACgcggc                               >  1:5437410/1‑150 (MQ=255)
                                                                                                                      ttGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACgcggc                               >  2:4633713/1‑150 (MQ=255)
                                                                                                                      ttGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACgcggc                               <  1:1225999/150‑1 (MQ=255)
                                                                                                                        gCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGcgtgcacgtagtca                             <  2:1571132/150‑7 (MQ=255)
                                                                                                                        gCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGgcgc                             <  1:5394620/150‑1 (MQ=255)
                                                                                                                         cccGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCgca                            <  1:3239360/150‑1 (MQ=255)
                                                                                                                         cccGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCgca                            <  2:8611/150‑1 (MQ=255)
                                                                                                                         cccGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCgca                            <  1:513185/150‑1 (MQ=255)
                                                                                                                          ccGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCTCGCGGCgcag                           <  2:1316470/150‑1 (MQ=255)
                                                                                                                          ccGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCgcag                           <  1:4452254/150‑1 (MQ=255)
                                                                                                                          ccGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCgcag                           <  2:3464009/150‑1 (MQ=255)
                                                                                                                                gCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTc                     <  1:4787859/150‑1 (MQ=255)
                                                                                                                                  gTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGa                   <  1:1721715/150‑1 (MQ=255)
                                                                                                                                  gTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGa                   <  1:1722172/150‑1 (MQ=255)
                                                                                                                                      gcccaGCTCTCCCGGTGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATAcc               >  1:99773/3‑150 (MQ=255)
                                                                                                                                       cccaGCTCTCCCGGTGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCg              >  1:4726163/2‑150 (MQ=255)
                                                                                                                                         caGCTCTCCCGGTGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGga            >  2:3400885/1‑150 (MQ=255)
                                                                                                                                         caGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGga            <  1:3985593/150‑1 (MQ=255)
                                                                                                                                         caGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGga            <  1:984056/150‑1 (MQ=255)
                                                                                                                                         caGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGga            <  2:404191/150‑1 (MQ=255)
                                                                                                                                               aCCGGGAGAGCTGGGCTTGGGTACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGAGATGcc      >  2:2755113/1‑150 (MQ=255)
                                                                                                                                                  gggAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGAGATGCCTgg   <  2:5062310/150‑1 (MQ=255)
                                                                                                                                                  gggAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGAGATGCCTgg   >  1:2555750/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCGCCCCAGCGCGCCTCGCGCCGCCCCGGCCACATTTTCAATGCGCCACTGCGTACCTGTTCAGGTACCTCGGGCGCGCTGGTTTCACCCATCAGGTATGGCTGCACGCGACGCAGTATTGCCCGCAGGCGTGCCACCAGCTCACCGGGAGAGCTGGGCTTGGGCACATAGTCATCGGCCCCCAGCTCCAGGCCGGCAATGCGGTCGATGTTGTCACCGCGTGCTGTCAGCAGCAGCACCGGCACCTGACTACGTGCACGCACGCGGCGCAGCACCTCGATACCGGAGATGCCTGGC  >  NC_002947/3818325‑3818621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑