Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,902,082 C→A 5.7% P175Q (CCG→CAG)  PP_3443 → glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,902,0820CA 5.7% 578.8 / 17.1 174P175Q (CCG→CAG) PP_3443glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (89/74);  new base A (3/7);  total (92/82)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 177 total aligned reads displayed.

ATCGGCAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGAAGC  >  NC_002947/3901934‑3902229
                                                                                                                                                    |                                                                                                                                                   
 tCGGCAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGc                                                                                                                                                   >  2:4440081/1‑150 (MQ=255)
    gCAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTgg                                                                                                                                                <  2:3214578/150‑1 (MQ=255)
    gCAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATAGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGTCACCCTCGGCCAGATCCGCCGCGCCCCGCTgg                                                                                                                                                <  2:3748837/150‑1 (MQ=255)
     cAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTggg                                                                                                                                               <  1:2363171/150‑1 (MQ=255)
         aaCCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTg                                                                                                                                           <  1:1938857/150‑1 (MQ=255)
          aCCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCACGCCCAGCGGGGCGCgg                                                                                                                                          <  1:1845307/150‑1 (MQ=255)
            cTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGcc                                                                                                                                        >  2:4416382/1‑150 (MQ=255)
            cTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGcc                                                                                                                                        <  1:2189691/150‑1 (MQ=255)
            cTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCACGCCCAGCGGGGCGCGGcg                                                                                                                                        <  1:3717400/150‑2 (MQ=255)
             tGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGccc                                                                                                                                       >  1:2730539/1‑150 (MQ=255)
             tGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGccc                                                                                                                                       >  1:3270216/1‑150 (MQ=255)
               aaGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCtg                                                                                                                                     >  2:5077159/1‑150 (MQ=255)
                 ggATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCtgta                                                                                                                                   >  2:3353513/1‑149 (MQ=255)
                  gATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGc                                                                                                                                  <  2:3102325/150‑1 (MQ=255)
                   aTTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCa                                                                                                                                 >  1:102400/1‑150 (MQ=255)
                    ttCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCAt                                                                                                                                >  1:3978088/1‑150 (MQ=255)
                    ttCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCAt                                                                                                                                >  1:3458857/1‑150 (MQ=255)
                     tCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATg                                                                                                                               >  2:3878414/1‑150 (MQ=255)
                        gAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATgggg                                                                                                                            <  2:102400/150‑1 (MQ=255)
                          aaaGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGcc                                                                                                                          >  2:3719630/1‑150 (MQ=255)
                            aGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCt                                                                                                                        <  1:1365412/150‑1 (MQ=255)
                             ggAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTa                                                                                                                       >  1:3543674/1‑150 (MQ=255)
                             ggAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTa                                                                                                                       >  2:4117600/1‑150 (MQ=255)
                             ggAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCCATGCACAGGGccacg                                                                                                                       <  2:2107548/150‑4 (MQ=255)
                                aTTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                    <  1:2615661/150‑1 (MQ=255)
                                aTTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                    <  1:4245492/150‑1 (MQ=255)
                                 ttCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAAc                                                                                                                   >  2:2816921/1‑150 (MQ=255)
                                  tCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACt                                                                                                                  >  2:2209484/1‑150 (MQ=255)
                                  tCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACt                                                                                                                  <  2:2021139/150‑1 (MQ=255)
                                  tCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACt                                                                                                                  <  1:5284188/150‑1 (MQ=255)
                                    gACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTAc                                                                                                                >  1:4763775/1‑150 (MQ=255)
                                     aCCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTAcc                                                                                                               >  1:4091380/1‑150 (MQ=255)
                                       cGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCg                                                                                                             <  1:3263711/150‑1 (MQ=255)
                                            cTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGaa                                                                                                        <  1:2923646/150‑1 (MQ=255)
                                             tGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAAc                                                                                                       <  2:414904/150‑1 (MQ=255)
                                                 aCTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACgaga                                                                                                   <  2:2948241/150‑1 (MQ=255)
                                                  cTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGAc                                                                                                  <  1:140331/150‑1 (MQ=255)
                                                   tACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCt                                                                                                                                      <  2:3162526/113‑1 (MQ=255)
                                                   tACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCt                                                                                                                                      >  1:3162526/1‑113 (MQ=255)
                                                   tACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGAcc                                                                                                 >  1:2241984/1‑150 (MQ=255)
                                                   tACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGAcc                                                                                                 >  2:2417446/1‑150 (MQ=255)
                                                         gtcgACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                    <  1:4200885/125‑1 (MQ=255)
                                                         gtcgACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACaa                                                                                                                    >  2:4200885/1‑125 (MQ=255)
                                                            gACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACg                                                                                        >  2:1883583/1‑150 (MQ=255)
                                                             acacCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGc                                                                                       >  2:1466340/1‑150 (MQ=255)
                                                              cacCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCt                                                                                      >  1:2638820/1‑150 (MQ=255)
                                                                  aTCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATc                                                                                  >  2:3829438/1‑150 (MQ=255)
                                                                  aTCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATc                                                                                  >  2:1707081/1‑150 (MQ=255)
                                                                  aTCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATc                                                                                  >  1:2913793/1‑150 (MQ=255)
                                                                   tCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATcc                                                                                 >  1:3375653/1‑150 (MQ=255)
                                                                      aCGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCgg                                                                              >  1:3401395/1‑150 (MQ=255)
                                                                       cGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGgc                                                                             <  2:3954526/150‑1 (MQ=255)
                                                                       cGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGgc                                                                             >  1:2538163/1‑150 (MQ=255)
                                                                       cGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGgc                                                                             >  1:1205687/1‑150 (MQ=255)
                                                                          ccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCGGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCCTCACCACGCTCATCCCGGCGCt                                                                          <  1:3586817/150‑1 (MQ=255)
                                                                          ccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTc                                                                                              >  2:1571113/1‑130 (MQ=255)
                                                                          ccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTc                                                                                              <  1:1571113/130‑1 (MQ=255)
                                                                          ccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCt                                                                          <  2:4188154/150‑1 (MQ=255)
                                                                          ccTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCt                                                                          <  1:3586979/150‑1 (MQ=255)
                                                                           cTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAAc                                                                                                       <  2:4016445/120‑1 (MQ=255)
                                                                            tCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGa                                                                        >  1:4531651/1‑150 (MQ=255)
                                                                             cAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCtt                                                                                               >  1:2492070/1‑126 (MQ=255)
                                                                             cAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCtt                                                                                               <  2:2492070/126‑1 (MQ=255)
                                                                             cAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCtt                                                                                               <  2:2492112/126‑1 (MQ=255)
                                                                             cAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGAt                                                                       <  2:4984750/150‑1 (MQ=255)
                                                                                ggACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCAt                                                                    >  1:5438370/1‑150 (MQ=255)
                                                                                ggACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCAt                                                                    >  1:4513080/1‑150 (MQ=255)
                                                                                 gACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATg                                                                   <  1:1116831/150‑1 (MQ=255)
                                                                                  aCCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATgg                                                                  >  2:1550074/1‑150 (MQ=255)
                                                                                  aCCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATgg                                                                  <  1:1287507/150‑1 (MQ=255)
                                                                                   ccTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATggg                                                                 <  2:1837930/150‑1 (MQ=255)
                                                                                   ccTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATggg                                                                 <  2:3269120/150‑1 (MQ=255)
                                                                                    cTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGc                                                                >  2:534778/1‑150 (MQ=255)
                                                                                     tGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCa                                                               >  1:854869/1‑150 (MQ=255)
                                                                                     tGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCa                                                               >  1:2095365/1‑150 (MQ=255)
                                                                                       gtgcctcgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAac                                                             >  2:458812/7‑150 (MQ=255)
                                                                                          cgccgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACg                                                          >  2:1143492/1‑150 (MQ=255)
                                                                                            ccgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACggt                                                        >  2:3117097/1‑150 (MQ=255)
                                                                                            ccgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACggt                                                        <  1:2417446/150‑1 (MQ=255)
                                                                                             cgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACggtg                                                       <  2:3417025/150‑1 (MQ=255)
                                                                                             cgcTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACggtg                                                       <  1:1487435/150‑1 (MQ=255)
                                                                                                tCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGgtggtg                                                    <  1:2877639/150‑1 (MQ=255)
                                                                                                tCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGgtggtg                                                    <  2:4358658/150‑1 (MQ=255)
                                                                                                        cTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGcc                                            >  2:610075/1‑150 (MQ=255)
                                                                                                         ttCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCg                                           <  2:5072193/150‑1 (MQ=255)
                                                                                                              gCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCaa                                      <  1:1696607/150‑1 (MQ=255)
                                                                                                                  gAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTc                                  >  1:5125027/1‑150 (MQ=255)
                                                                                                                         gCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTAc                           <  2:3530128/150‑1 (MQ=255)
                                                                                                                            cccTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTActgc                        <  2:3695531/150‑1 (MQ=255)
                                                                                                                               tCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGa                     >  1:334761/1‑150 (MQ=255)
                                                                                                                                 ggccttttCCGCGGCGCCCGGCTGGGGGTGGCCCTGTGCTTGGGGCCCTACAACTACCGGCTGAACGAGACCTTCACCAGGCTCTTCCGGGGGTTTTTTTTGGGCAACAGGTTGGTGTTAATGCGGGCAATTTTCGGCTTACTGCTTATc                   <  2:4862152/143‑1 (MQ=255)
                                                                                                                                 ggCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATc                   >  2:4538693/1‑150 (MQ=255)
                                                                                                                                     aGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGAc               <  1:611949/150‑1 (MQ=255)
                                                                                                                                       aTCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGAccc             <  1:2032075/150‑1 (MQ=255)
                                                                                                                                        agggccacGCCCAGCGGGGCGCGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCaa                                      >  1:4377715/4‑124 (MQ=255)
                                                                                                                                          ggccacGCCCAGCGGGGCGCGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTAGGCGTACTGCTGATCCGACCCTTg          >  1:1744143/2‑150 (MQ=255)
                                                                                                                                           gccgcGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCATGTTCGGCGTACTGCTGATCCGACCCTTGc         <  1:4895424/150‑1 (MQ=255)
                                                                                                                                                gcCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGaa    <  2:1407374/150‑1 (MQ=255)
                                                                                                                                                gcCCAGCGGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACgaga                                                                                                   <  1:2182835/55‑1 (MQ=255)
                                                                                                                                                 ccccGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGAAg   <  2:723877/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCGGCAAGAACCTGAAGGATTCGGAAAAGGAATTCGACCGCACCTGCGACTACATCGTCGACACCATCAACGCCCTCAAGGACCTGGACCGCCGCTCCAGCCGCTTCGAGCTGGAGCAAGGCACCCTCGGCCAGATCCGCCGCGCCCCGCTGGGCGTGGCCCTGTGCATGGGGCCCTACAACTACCCGCTGAACGAGACCTTCACCACGCTCATCCCGGCGCTGATCATGGGCAACACGGTGGTGTTCAAGCCGGCCAAGTTCGGCGTACTGCTGATCCGACCCTTGCTCGAAGC  >  NC_002947/3901934‑3902229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑