BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,238,514	A→T	5.3%	A75A (GCT→GCA) ‡	catA‑I ←	catechol 1,2‑dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,238,514	0	A	T	5.3%	578.9 / 14.8	170	A75A (GCT→GCA) ‡	catA‑I	catechol 1,2‑dioxygenase
Reads supporting (aligned to +/- strand): ref base A (71/90); new base T (4/5); total (75/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 189 total aligned reads displayed.

TCGTCCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGTTTCC  >  NC_002947/4238365‑4238663

tcgtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagca                                                                                                                                                       <  2:1834587/150‑1 (MQ=255)

tcgtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagca                                                                                                                                                       >  2:1887142/1‑150 (MQ=255)

  gtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagcagc                                                                                                                                                     <  1:3826793/150‑1 (MQ=255)

  gtcCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagcagc                                                                                                                                                     >  2:4670524/1‑150 (MQ=255)

      aTGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGgccagcagccagc                                                                                                                                                 >  2:3506716/1‑150 (MQ=255)

        gcgcgcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGccagcagccagcag                                                                                                                                               <  1:2262124/150‑1 (MQ=255)

           cgcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgcc                                                                                                                                            >  1:324418/1‑150 (MQ=255)

            gcTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgcct                                                                                                                                           >  2:4330590/1‑150 (MQ=255)

              ttCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCTGCTGGCTGCTGgcctgg                                                                                                                                         <  1:2393561/150‑2 (MQ=37)

              ttCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGgcctgc                                                                                                                                         <  2:1502884/150‑1 (MQ=255)

                  cccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCTGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCg                                                                                                                                     <  2:1467408/150‑1 (MQ=255)

                   ccTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGt                                                                                                                                    >  2:3531213/1‑150 (MQ=255)

                        gCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACgg                                                                                                                               >  1:660474/1‑150 (MQ=255)

                         ccAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGgc                                                                                                                              >  1:2790714/1‑150 (MQ=255)

                          cAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGgcc                                                                                                                             <  1:101161/150‑1 (MQ=255)

                               gcgcCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGATTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCa                                                                                                                        <  2:3540000/150‑1 (MQ=255)

                                 gcCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAgg                                                                                                                      >  1:1486565/1‑150 (MQ=255)

                                  ccccGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGc                                                                                                                     <  1:5094455/150‑1 (MQ=255)

                                   cccGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCg                                                                                                                    <  1:615385/150‑1 (MQ=255)

                                        cAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGa                                                                                                               <  2:4603390/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:1681681/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:91806/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:3845824/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:617319/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  1:4716402/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:3035731/150‑1 (MQ=255)

                                         aaCGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCATCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  1:416036/150‑1 (MQ=255)

                                                      ccTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCg                                                                                                 <  1:2845598/150‑1 (MQ=255)

                                                       cTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGTCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGt                                                                                                <  1:3171332/150‑1 (MQ=255)

                                                        ttCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAgg                                                                                                             >  1:5320622/1‑136 (MQ=255)

                                                        ttCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAgg                                                                                                             <  2:5320622/136‑1 (MQ=255)

                                                          cGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGcc                                                                                             <  1:347705/150‑1 (MQ=255)

                                                           gATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTTGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCa                                                                                            <  1:624782/150‑1 (MQ=255)

                                                             tGGTGCGCGGGGTGCCGCCGCCAAGGCCTGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGa                                                                                          >  2:525109/1‑150 (MQ=255)

                                                              ggTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGaa                                                                                         <  2:1376995/150‑1 (MQ=255)

                                                              ggTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGaa                                                                                         <  2:673160/150‑1 (MQ=255)

                                                                tGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACt                                                                                       >  1:350588/1‑150 (MQ=255)

                                                                 gcgcGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTc                                                                                      <  1:91181/150‑1 (MQ=255)

                                                                    cgGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTc                                                                                   <  1:321783/150‑1 (MQ=255)

                                                                    aaggatGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              >  1:2162876/6‑123 (MQ=255)

                                                                    aaggatGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:2162876/118‑1 (MQ=255)

                                                                        gTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCg                                                                               <  1:373975/150‑1 (MQ=255)

                                                                         tGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACg                                                                                                    >  2:1953438/1‑128 (MQ=255)

                                                                           ccgccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                              >  2:5121919/1‑132 (MQ=255)

                                                                           ccgccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGc                                                                                              <  1:5121919/132‑1 (MQ=255)

                                                                           ccgccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTa                                                                            <  2:5196668/150‑1 (MQ=255)

                                                                             gccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAAt                                                                          <  1:4659792/150‑1 (MQ=255)

                                                                              ccgccAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAAtc                                                                         >  2:1970344/1‑150 (MQ=255)

                                                                                  cAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCa                                                                     <  1:1908723/150‑1 (MQ=255)

                                                                                  cAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCa                                                                     <  1:2193122/150‑1 (MQ=255)

                                                                                   aaGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAg                                                                    <  1:4711527/150‑1 (MQ=255)

                                                                                   aaGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAg                                                                    >  1:1591565/1‑150 (MQ=255)

                                                                                    aGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAgg                                                                   >  2:3017928/1‑150 (MQ=255)

                                                                                     ggCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGt                                                                  >  2:4950654/1‑150 (MQ=255)

                                                                                       ccGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCt                                                                >  2:2527074/1‑150 (MQ=255)

                                                                                       ccGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCt                                                                <  2:3208008/150‑1 (MQ=255)

                                                                                        cGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCtt                                                               <  1:1131044/150‑1 (MQ=255)

                                                                                          gCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCg                                                             <  1:5055683/150‑1 (MQ=255)

                                                                                              cGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCa                                                         <  2:2370706/150‑1 (MQ=255)

                                                                                                 cATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggc                                                      <  1:2976986/150‑1 (MQ=255)

                                                                                                   tCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcgg                                                    <  1:2897559/150‑1 (MQ=255)

                                                                                                    ccTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcggg                                                   >  1:5328365/1‑150 (MQ=255)

                                                                                                      ttGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcgggcg                                                 >  1:3689789/1‑150 (MQ=255)

                                                                                                        ggCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAggcgtggtga                                               <  2:1150072/150‑7 (MQ=255)

                                                                                                        ggCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGgt                                               <  2:3658764/150‑1 (MQ=255)

                                                                                                           aTCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTc                                            >  1:3585322/1‑150 (MQ=255)

                                                                                                             ccTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGTTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCtt                                          <  1:5155380/150‑1 (MQ=255)

                                                                                                             ccTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCtt                                          >  1:1451296/1‑150 (MQ=255)

                                                                                                                gcagcaGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTgca                                       >  1:4522786/1‑150 (MQ=255)

                                                                                                                  agcagGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTgcagc                                     >  1:3575882/1‑150 (MQ=255)

                                                                                                                       gTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCa                                                         <  2:2693563/125‑1 (MQ=255)

                                                                                                                       gTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCAcgcg                                >  1:1697584/1‑150 (MQ=255)

                                                                                                                       gTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCAcgcg                                >  2:1619628/1‑150 (MQ=255)

                                                                                                                        tCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACgcgc                               >  2:3916646/1‑150 (MQ=255)

                                                                                                                          gAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAg                             >  2:4217424/1‑150 (MQ=255)

                                                                                                                             gAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAAt                          >  1:3323641/1‑150 (MQ=255)

                                                                                                                                gTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGAt                       <  1:4670524/150‑1 (MQ=255)

                                                                                                                                 tGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATc                      <  1:4420992/150‑1 (MQ=255)

                                                                                                                                  gCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTCGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCt                     >  1:4185340/1‑150 (MQ=255)

                                                                                                                                   cTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTg                    <  2:2946165/150‑1 (MQ=255)

                                                                                                                                      gATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCtt                 >  2:4567404/1‑150 (MQ=255)

                                                                                                                                         aCCCATGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCTGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCATAATGATCTGATTGaa              <  2:1165214/150‑1 (MQ=255)

                                                                                                                                           ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAg                                                                                                              <  2:3228576/52‑1 (MQ=255)

                                                                                                                                           ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTgca                                       >  2:2818527/1‑123 (MQ=255)

                                                                                                                                           ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgc            >  1:1993707/1‑150 (MQ=255)

                                                                                                                                           ccAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgc            >  2:2249270/1‑150 (MQ=255)

                                                                                                                                            cAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgcg           >  2:3732891/1‑150 (MQ=255)

                                                                                                                                            cAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgcg           >  2:4252826/1‑150 (MQ=255)

                                                                                                                                             aGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGaa                           >  2:3235622/1‑133 (MQ=25)

                                                                                                                                             aGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGaa                           <  1:3235546/133‑1 (MQ=25)

                                                                                                                                             aGGCCTGCTGGCTGCTGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgcgc          >  1:5436249/1‑150 (MQ=37)

                                                                                                                                             aGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAgcgc          >  1:2421598/1‑150 (MQ=255)

                                                                                                                                               gccagcagccagcaGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCgg        <  1:5257375/150‑1 (MQ=255)

                                                                                                                                                 cagcagccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGt      >  1:1073658/1‑150 (MQ=255)

                                                                                                                                                  agcagccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGtt     <  1:2688629/150‑1 (MQ=255)

                                                                                                                                                   gcagccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGttt    >  1:461959/1‑150 (MQ=255)

                                                                                                                                                   gcagccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGttt    <  2:4704634/150‑1 (MQ=255)

                                                                                                                                                     agccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGTTTcc  <  1:48785/150‑1 (MQ=255)

                                                                                                                                                     agccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGTTTcc  >  2:3092085/1‑150 (MQ=255)

                                                                                                                                                     agccagcagGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGTTTcc  <  1:2041015/150‑1 (MQ=255)

TCGTCCATGCGCGCTTCGCCCTGGGCCAGCGGCGCCCCGGCAACGTACAACGGGCCTTCGATGGTGCGCGGGGTGCCGCCGCCAAGGCCGGCTTCGGCATCCTTGGCATCCTGCAGCAGGTCGAGGAAGTGCTCGATACCCAGGCCAGCAGCCAGCAGGCCTGCCTCGTTACGGCCGCCCAGGCGGTTGAGGTAGTCGACGGCGTGCCAGAACTCGTCCTCGGTAATCTCCAGGTCTTCGATCAGGCGGGCGGTGTCTTGCAGCACGCGCAGAATGATCTGCTTGAAGCGCGGGTTTCC  >  NC_002947/4238365‑4238663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`