BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,307,573	G→A	6.3%	Q150* (CAG→TAG)	PP_3777 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,307,573	0	G	A	6.3%	539.8 / 16.5	175	Q150* (CAG→TAG)	PP_3777	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (80/84); new base A (2/9); total (82/93)
Fisher's exact test for biased strand distribution p-value = 6.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 179 total aligned reads displayed.

ACCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCT  >  NC_002947/4307424‑4307719

acCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTg                                                                                                                                                     >  2:2889060/1‑150 (MQ=255)

acCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTg                                                                                                                                                     >  2:2889232/1‑150 (MQ=255)

acCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTg                                                                                                                                                     >  2:4895266/1‑150 (MQ=255)

  cGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTa                                                                                                                                                   <  1:3505196/150‑1 (MQ=255)

  cGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTa                                                                                                                                                   <  2:4878906/150‑1 (MQ=255)

           cgtccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGa                                                                                                                                          <  1:4992212/147‑1 (MQ=255)

            gACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAg                                                                                                                                         >  2:3006766/1‑150 (MQ=255)

             aCCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAgg                                                                                                                                        >  2:5425689/1‑150 (MQ=255)

             aCCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAgg                                                                                                                                        <  2:3230645/150‑1 (MQ=255)

               cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      <  1:4451795/150‑1 (MQ=255)

               cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      >  1:4360694/1‑150 (MQ=255)

               cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      >  2:3156758/1‑150 (MQ=255)

               cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      <  1:4973195/150‑1 (MQ=255)

                aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCTTCCTCGATGCACTACAGCGCATCGAggctg                                                                                                                                     <  2:4994095/150‑4 (MQ=255)

                aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                     >  1:4956488/1‑150 (MQ=255)

                aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                     >  1:4956484/1‑150 (MQ=255)

                  cGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGcc                                                                                                                                   <  2:4438410/150‑1 (MQ=255)

                  cGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGcc                                                                                                                                   <  1:617860/150‑1 (MQ=255)

                   gAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCa                                                                                                                                  >  2:4855608/1‑150 (MQ=255)

                        cAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGa                                                                                                                             <  2:3818744/150‑1 (MQ=255)

                          gCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAgg                                                                                                                           <  2:4571598/150‑1 (MQ=255)

                           cccGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAggg                                                                                                                          >  1:1926884/1‑150 (MQ=255)

                              gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      <  1:2724763/135‑1 (MQ=255)

                              gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      >  2:2724763/1‑135 (MQ=255)

                              gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                       >  1:218748/1‑150 (MQ=255)

                                tgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCt                                                                                                                     <  1:192082/150‑1 (MQ=255)

                                   tCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGcc                                                                                                                  >  1:1087150/1‑150 (MQ=255)

                                     aGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAc                                                                                                                >  2:422223/1‑150 (MQ=255)

                                      gCTTGCCTCCTGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               >  2:4315065/1‑150 (MQ=255)

                                      gCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               >  2:1621340/1‑150 (MQ=255)

                                            cTCCGGGCTTCAGGTAGCTCGGCACCCGATCGAAAAAACTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGAAGCCCTGAAGAGCATCGAGGAAGCCATCCGAGGGCGACTGCCACCCTGGCg                                                                                                         >  2:2389182/1‑150 (MQ=255)

                                                gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGGCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     <  1:261104/150‑1 (MQ=255)

                                                gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     <  1:950326/150‑1 (MQ=255)

                                                gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     <  2:2486479/150‑1 (MQ=255)

                                                gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     >  2:3844754/1‑150 (MQ=255)

                                                gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     <  1:4061967/150‑1 (MQ=255)

                                                   cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGCGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  <  2:4075981/150‑1 (MQ=255)

                                                   cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  <  1:2755076/150‑1 (MQ=255)

                                                   cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  <  1:2754863/150‑1 (MQ=255)

                                                   cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  >  1:5376917/1‑150 (MQ=255)

                                                    ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAAt                                                                                                 >  1:2713131/1‑150 (MQ=255)

                                                    ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAAt                                                                                                 >  1:2043118/1‑150 (MQ=255)

                                                     tCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATa                                                                                                <  2:303270/150‑1 (MQ=255)

                                                     tCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATa                                                                                                <  1:1621340/150‑1 (MQ=255)

                                                       aGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAgg                                                                                              <  2:923758/150‑1 (MQ=255)

                                                         gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTAGCGGATTGAATAGGAt                                                                                            <  2:2201173/150‑1 (MQ=255)

                                                            gCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATcac                                                                                         >  1:2981854/1‑150 (MQ=255)

                                                             cTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATcaca                                                                                        <  1:3181843/150‑1 (MQ=255)

                                                              tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                       >  2:5282232/1‑150 (MQ=255)

                                                              tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                       >  1:1228234/1‑150 (MQ=255)

                                                                     ccGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATcc                                                                                >  2:2752276/1‑150 (MQ=255)

                                                                      cGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCg                                                                               <  1:1052784/150‑1 (MQ=255)

                                                                           gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  2:640762/1‑150 (MQ=255)

                                                                           gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  1:4130986/1‑150 (MQ=255)

                                                                           gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  2:2288642/1‑150 (MQ=255)

                                                                           gAAAAACCTGCGCAAGGTCCGCACGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  2:4659278/1‑150 (MQ=255)

                                                                                 ccTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGc                                                                    >  2:3418399/1‑150 (MQ=255)

                                                                                     cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   >  1:5033209/1‑147 (MQ=255)

                                                                                     cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   <  2:5033209/147‑1 (MQ=255)

                                                                                     cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGc                                                                >  1:824499/1‑150 (MQ=255)

                                                                                       cAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                              >  2:1667411/1‑150 (MQ=255)

                                                                                        aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                             <  2:2427276/150‑1 (MQ=255)

                                                                                          ggTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                           <  2:1389395/150‑1 (MQ=255)

                                                                                          ggTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                           <  2:4461902/150‑1 (MQ=255)

                                                                                              cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCg                                                       >  2:2347168/1‑150 (MQ=255)

                                                                                               gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGa                                                      >  1:5412480/1‑150 (MQ=255)

                                                                                                cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTTCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  1:2389182/150‑1 (MQ=255)

                                                                                                cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGTGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  1:3347352/150‑1 (MQ=255)

                                                                                                cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  2:824499/150‑1 (MQ=255)

                                                                                                cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  1:3912496/150‑1 (MQ=255)

                                                                                                 tCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAAc                                                    <  1:5101729/150‑1 (MQ=255)

                                                                                                     cATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAt                                                >  2:3872725/1‑150 (MQ=255)

                                                                                                      aTAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                               >  2:3405317/1‑150 (MQ=255)

                                                                                                       tAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               <  2:4114696/85‑1 (MQ=255)

                                                                                                       tAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               >  1:4114696/1‑85 (MQ=255)

                                                                                                          aaCATCGCTTCCAGCGACAGCCCTTGGGCTGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTccc                                           <  1:4690069/150‑1 (MQ=255)

                                                                                                           aCATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                          >  2:1897280/1‑150 (MQ=255)

                                                                                                            cATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTg                                         >  2:1995663/1‑150 (MQ=255)

                                                                                                              tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                       >  1:932332/1‑150 (MQ=255)

                                                                                                              tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                       >  2:3964862/1‑150 (MQ=255)

                                                                                                               cGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑c                                     <  1:3139231/150‑1 (MQ=255)

                                                                                                                  ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   >  1:2486730/1‑118 (MQ=255)

                                                                                                                  ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGaa                                  >  2:4548939/1‑150 (MQ=255)

                                                                                                                      aGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCg                              >  1:1688348/1‑150 (MQ=255)

                                                                                                                         gACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATa                           <  1:3006766/150‑1 (MQ=255)

                                                                                                                           cAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACa                         <  1:2897780/150‑1 (MQ=255)

                                                                                                                            aGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAg                        <  2:2160430/150‑1 (MQ=255)

                                                                                                                             gCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTATCGAATTCG‑TACAgg                       <  2:1649919/150‑1 (MQ=255)

                                                                                                                              cccTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGATGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTATGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGa                      >  1:2372011/1‑150 (MQ=255)

                                                                                                                               ccTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGAt                     >  1:4477719/1‑150 (MQ=255)

                                                                                                                                cTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGAtt                    >  1:3756876/1‑150 (MQ=255)

                                                                                                                                     gCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAc                                                                                                                <  1:3098973/54‑1 (MQ=255)

                                                                                                                                           tCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTg         >  1:4718472/1‑150 (MQ=255)

                                                                                                                                           tCGATGCACTACAGCGCATCGAGGCTGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGAGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTg         >  2:1322243/1‑150 (MQ=255)

                                                                                                                                             gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc       <  2:4737178/150‑1 (MQ=255)

                                                                                                                                             gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc       <  1:4252146/150‑1 (MQ=255)

                                                                                                                                             gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc       >  2:3951882/1‑150 (MQ=255)

                                                                                                                                                  gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                              >  2:3188714/1‑91 (MQ=255)

                                                                                                                                                  gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                              <  1:3188714/91‑1 (MQ=255)

                                                                                                                                                  gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCt  <  1:2330365/150‑1 (MQ=255)

ACCGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCT  >  NC_002947/4307424‑4307719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`