BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,307,574	T→C	6.3%	L149L (CTA→CTG)	PP_3777 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,307,574	0	T	C	6.3%	547.0 / 14.2	175	L149L (CTA→CTG)	PP_3777	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (79/84); new base C (2/9); total (82/93)
Fisher's exact test for biased strand distribution p-value = 6.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCT  >  NC_002947/4307426‑4307719

cGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTa                                                                                                                                                   <  2:4878906/150‑1 (MQ=255)

cGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTa                                                                                                                                                   >  1:3253874/1‑150 (MQ=255)

         cgtccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGa                                                                                                                                          <  1:4992212/147‑1 (MQ=255)

         cGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGa                                                                                                                                          <  1:4572443/150‑1 (MQ=255)

             cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      >  2:3156758/1‑150 (MQ=255)

             cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      >  1:4360694/1‑150 (MQ=255)

             cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      <  1:4973195/150‑1 (MQ=255)

             cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      >  2:2330365/1‑150 (MQ=255)

              aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCTTCCTCGATGCACTACAGCGCATCGAggctg                                                                                                                                     <  2:4994095/150‑4 (MQ=255)

              aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                     >  1:4956484/1‑150 (MQ=255)

              aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                     >  1:4956611/1‑150 (MQ=255)

                cGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGcc                                                                                                                                   <  2:4438410/150‑1 (MQ=255)

                cGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGcc                                                                                                                                   <  1:617860/150‑1 (MQ=255)

                      cAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGa                                                                                                                             <  2:3818744/150‑1 (MQ=255)

                        gCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAgg                                                                                                                           <  2:4571598/150‑1 (MQ=255)

                         cccGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAggg                                                                                                                          >  1:1926884/1‑150 (MQ=255)

                           cGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCg                                                                                                                        >  1:5328273/1‑150 (MQ=255)

                            gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                      <  1:2724763/135‑1 (MQ=255)

                            gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                       <  1:2279941/150‑1 (MQ=255)

                            gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                       >  1:218748/1‑150 (MQ=255)

                              tgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCt                                                                                                                     <  1:192082/150‑1 (MQ=255)

                                 tCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGcc                                                                                                                  >  1:1087150/1‑150 (MQ=255)

                                   aGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAc                                                                                                                >  2:422223/1‑150 (MQ=255)

                                    gCTTGCCTCCTGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               >  2:4315065/1‑150 (MQ=255)

                                    gCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               >  2:3026213/1‑150 (MQ=255)

                                        gCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCCTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                     >  1:3174726/1‑124 (MQ=255)

                                          cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAACACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                         <  2:275872/150‑1 (MQ=255)

                                          cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                         >  2:2374183/1‑150 (MQ=255)

                                           tCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                       <  2:634529/135‑1 (MQ=255)

                                              gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     <  2:2486479/150‑1 (MQ=255)

                                              gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     <  1:950326/150‑1 (MQ=255)

                                              gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                     >  2:3844754/1‑150 (MQ=255)

                                                 cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGCGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  <  2:4075981/150‑1 (MQ=255)

                                                 cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  <  1:2754944/150‑1 (MQ=255)

                                                 cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  >  1:5376917/1‑150 (MQ=255)

                                                 cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                  <  1:2754863/150‑1 (MQ=255)

                                                  ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAAt                                                                                                 >  1:2043118/1‑150 (MQ=255)

                                                   tCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATa                                                                                                <  2:303270/150‑1 (MQ=255)

                                                   tCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATa                                                                                                <  1:1621340/150‑1 (MQ=255)

                                                     aGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAgg                                                                                              <  2:923758/150‑1 (MQ=255)

                                                       gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGAt                                                                                            >  1:4473812/1‑150 (MQ=255)

                                                       gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGAt                                                                                            >  2:1958406/1‑150 (MQ=255)

                                                       gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTAGCGGATTGAATAGGAt                                                                                            <  2:2201173/150‑1 (MQ=255)

                                                            tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                       >  2:5282232/1‑150 (MQ=255)

                                                            tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                       >  1:2677035/1‑150 (MQ=255)

                                                            tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                       >  1:1228234/1‑150 (MQ=255)

                                                            tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                       >  2:1443086/1‑150 (MQ=255)

                                                            tCGGCACCCGTTCGAAAAACCTGCCCAAGGTCCGCTCGCCATAGAACAACGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGAGGAATGAATAGGATcacaa                                                                                       >  1:4118037/1‑149 (MQ=255)

                                                             cGGCACCCGTTCGAAAAACCTGCGCTAGGTCCGCTCGCCAGAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATc                                                                                      <  2:4753755/150‑1 (MQ=255)

                                                                   ccGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATcc                                                                                >  2:2752111/1‑150 (MQ=255)

                                                                    cGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCg                                                                               <  1:1052784/150‑1 (MQ=255)

                                                                         gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  2:640762/1‑150 (MQ=255)

                                                                         gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  1:4131034/1‑150 (MQ=255)

                                                                         gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  2:2288642/1‑150 (MQ=255)

                                                                         gAAAAACCTGCGCAAGGTCCGCACGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                          >  2:4659278/1‑150 (MQ=255)

                                                                           aaaaCCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTg                                                                        <  1:2976584/150‑1 (MQ=255)

                                                                               ccTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGc                                                                    >  2:3418399/1‑150 (MQ=255)

                                                                                   cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   <  2:5033209/147‑1 (MQ=255)

                                                                                   cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   >  1:5033209/1‑147 (MQ=255)

                                                                                   cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGc                                                                >  1:824499/1‑150 (MQ=255)

                                                                                     cAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                              >  2:1667411/1‑150 (MQ=255)

                                                                                      aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGTGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                             >  2:3347352/1‑150 (MQ=255)

                                                                                      aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                             <  1:3578578/150‑1 (MQ=255)

                                                                                      aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                             <  2:2427276/150‑1 (MQ=255)

                                                                                        ggTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                           <  2:1389395/150‑1 (MQ=255)

                                                                                            cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCg                                                       >  1:2576598/1‑150 (MQ=255)

                                                                                             gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGa                                                      >  1:5412480/1‑150 (MQ=255)

                                                                                              cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTTCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  1:2389182/150‑1 (MQ=255)

                                                                                              cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  1:3912496/150‑1 (MQ=255)

                                                                                              cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  1:3026213/150‑1 (MQ=255)

                                                                                              cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                     <  2:824499/150‑1 (MQ=255)

                                                                                                   cATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAt                                                >  2:3872725/1‑150 (MQ=255)

                                                                                                    aTAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                               >  2:4896294/1‑150 (MQ=255)

                                                                                                    aTAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                               >  2:3405317/1‑150 (MQ=255)

                                                                                                     tAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                               <  2:4114696/85‑1 (MQ=255)

                                                                                                      aGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtctc                                             <  1:3872725/150‑1 (MQ=255)

                                                                                                        aaCATCGCTTCCAGCGACAGCCCTTGGGCTGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTccc                                           <  1:4690069/150‑1 (MQ=255)

                                                                                                         aCATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACGTCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                          <  1:342703/150‑1 (MQ=255)

                                                                                                         aCATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                          >  2:1897280/1‑150 (MQ=255)

                                                                                                            tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                       >  2:3964862/1‑150 (MQ=255)

                                                                                                            tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                       >  1:932332/1‑150 (MQ=255)

                                                                                                            tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                       <  1:3114236/150‑1 (MQ=255)

                                                                                                                ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   <  2:2486730/118‑1 (MQ=255)

                                                                                                                ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                   >  1:2486730/1‑118 (MQ=255)

                                                                                                                ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGaa                                  >  1:278526/1‑150 (MQ=255)

                                                                                                                ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGaa                                  >  2:4548939/1‑150 (MQ=255)

                                                                                                                   cAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTc                               <  1:4087856/150‑1 (MQ=255)

                                                                                                                   cAGCGACAGCCCGTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTc                               <  2:4447473/150‑1 (MQ=255)

                                                                                                                    aGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCg                              >  1:1688348/1‑150 (MQ=255)

                                                                                                                       gACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATa                           <  1:3006766/150‑1 (MQ=255)

                                                                                                                          aGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAg                        <  2:4726949/150‑1 (MQ=255)

                                                                                                                           gCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTATCGAATTCG‑TACAgg                       <  2:1649919/150‑1 (MQ=255)

                                                                                                                            cccTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGATGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTATGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGa                      >  1:2372011/1‑150 (MQ=255)

                                                                                                                                      gCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAgg            <  2:2065121/150‑1 (MQ=255)

                                                                                                                                         tCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTg         >  1:4718472/1‑150 (MQ=255)

                                                                                                                                         tCGATGCACTACAGCGCATCGAGGCTGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGAGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTg         >  2:1322243/1‑150 (MQ=255)

                                                                                                                                           gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc       >  2:3951882/1‑150 (MQ=255)

                                                                                                                                           gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc       <  1:4252146/150‑1 (MQ=255)

                                                                                                                                                gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                              >  2:3188714/1‑91 (MQ=255)

                                                                                                                                                gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                              <  1:3188714/91‑1 (MQ=255)

CGGCAATGCCGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCT  >  NC_002947/4307426‑4307719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`