BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,307,577	T→C	6.3%	A148A (GCA→GCG)	PP_3777 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,307,577	0	T	C	6.3%	552.2 / 14.8	175	A148A (GCA→GCG)	PP_3777	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (80/83); new base C (2/9); total (83/92)
Fisher's exact test for biased strand distribution p-value = 6.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

CGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCATTGG  >  NC_002947/4307435‑4307725

cgtccAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGa                                                                                                                                                <  1:4992212/147‑1 (MQ=255)

cGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGa                                                                                                                                                <  1:4572443/150‑1 (MQ=255)

 gACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCACTACAGCGCATCGAg                                                                                                                                               <  2:4613379/150‑1 (MQ=255)

    cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                            >  2:3156758/1‑150 (MQ=255)

    cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                            >  1:4360694/1‑150 (MQ=255)

    cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                            <  1:4973195/150‑1 (MQ=255)

    cAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGaa                                                                                                                                            >  2:2330365/1‑150 (MQ=255)

     aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCTTCCTCGATGCACTACAGCGCATCGAggctg                                                                                                                                           <  2:4994095/150‑4 (MQ=255)

     aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                           >  1:4956484/1‑150 (MQ=255)

     aaCGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAg                                                                                                                                           >  1:4956611/1‑150 (MQ=255)

       cGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGcc                                                                                                                                         <  1:617860/150‑1 (MQ=255)

             cAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGa                                                                                                                                   <  2:3818744/150‑1 (MQ=255)

               gCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAgg                                                                                                                                 <  2:4571598/150‑1 (MQ=255)

                cccGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAggg                                                                                                                                >  1:1926884/1‑150 (MQ=255)

                  cGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCg                                                                                                                              >  1:5328273/1‑150 (MQ=255)

                   gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                             >  1:218748/1‑150 (MQ=255)

                   gatgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                             <  1:2279941/150‑1 (MQ=255)

                     tgatCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCt                                                                                                                           <  1:192082/150‑1 (MQ=255)

                        tCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGcc                                                                                                                        >  1:1087150/1‑150 (MQ=255)

                          aGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAc                                                                                                                      >  2:422223/1‑150 (MQ=255)

                           gCTTGCCTCCTGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                                     >  2:4315065/1‑150 (MQ=255)

                           gCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                                     >  2:3026213/1‑150 (MQ=255)

                                 cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAACACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                               <  2:275872/150‑1 (MQ=255)

                                 cTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCg                                                                                                               >  2:2374183/1‑150 (MQ=255)

                                  tCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGt                                                                                                                             <  2:634529/135‑1 (MQ=255)

                                     gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                           <  2:2486479/150‑1 (MQ=255)

                                     gggCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGAtt                                                                                                           <  1:950326/150‑1 (MQ=255)

                                        cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                        <  1:2755076/150‑1 (MQ=255)

                                        cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                        <  1:2754863/150‑1 (MQ=255)

                                        cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                        <  1:2754826/150‑1 (MQ=255)

                                        cTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGaa                                                                                                        >  1:5376917/1‑150 (MQ=255)

                                         ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAAt                                                                                                       >  1:2713131/1‑150 (MQ=255)

                                         ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAAt                                                                                                       >  1:2043118/1‑150 (MQ=255)

                                         ttCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAAt                                                                                                       <  2:3831720/150‑1 (MQ=255)

                                          tCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATa                                                                                                      <  2:303270/150‑1 (MQ=255)

                                          tCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATa                                                                                                      <  1:1621340/150‑1 (MQ=255)

                                            aGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAgg                                                                                                    <  2:923758/150‑1 (MQ=255)

                                              gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGAt                                                                                                  >  2:1958406/1‑150 (MQ=255)

                                              gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGAt                                                                                                  >  1:4473812/1‑150 (MQ=255)

                                              gTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTAGCGGATTGAATAGGAt                                                                                                  <  2:2201173/150‑1 (MQ=255)

                                                 gCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATcac                                                                                               >  1:2981854/1‑150 (MQ=255)

                                                  cTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATcaca                                                                                              <  1:3181843/150‑1 (MQ=255)

                                                   tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                             >  1:1228234/1‑150 (MQ=255)

                                                   tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                             >  2:5282232/1‑150 (MQ=255)

                                                   tCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACAt                                                                                             >  2:1443086/1‑150 (MQ=255)

                                                   tCGGCACCCGTTCGAAAAACCTGCCCAAGGTCCGCTCGCCATAGAACAACGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGAGGAATGAATAGGATcacaa                                                                                             >  1:4118037/1‑149 (MQ=255)

                                                    cGGCACCCGTTCGAAAAACCTGCGCTAGGTCCGCTCGCCAGAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATc                                                                                            <  2:4753755/150‑1 (MQ=255)

                                                          ccGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATcc                                                                                      >  2:2752111/1‑150 (MQ=255)

                                                           cGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCg                                                                                     <  1:1052784/150‑1 (MQ=255)

                                                                gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                                >  2:640762/1‑150 (MQ=255)

                                                                gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                                >  2:2288642/1‑150 (MQ=255)

                                                                gAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCctc                                                                                >  1:4131034/1‑150 (MQ=255)

                                                                      ccTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGc                                                                          >  2:3418399/1‑150 (MQ=255)

                                                                          cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                         <  2:5033209/147‑1 (MQ=255)

                                                                          cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                         >  1:5033209/1‑147 (MQ=255)

                                                                          cgcAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGc                                                                      >  1:824499/1‑150 (MQ=255)

                                                                            cAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                                    >  2:1667411/1‑150 (MQ=255)

                                                                             aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGTGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                                   >  2:3347352/1‑150 (MQ=255)

                                                                             aaGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAtt                                                                   <  2:2427276/150‑1 (MQ=255)

                                                                               ggTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGc                                                                 <  2:1389395/150‑1 (MQ=255)

                                                                                   cGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCg                                                             >  1:2576598/1‑150 (MQ=255)

                                                                                    gCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGa                                                            >  1:5412480/1‑150 (MQ=255)

                                                                                     cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTTCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                           <  1:2389182/150‑1 (MQ=255)

                                                                                     cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                           <  2:824499/150‑1 (MQ=255)

                                                                                     cTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGaa                                                           <  1:3912496/150‑1 (MQ=255)

                                                                                          cATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAt                                                      >  2:4327627/1‑150 (MQ=255)

                                                                                          cATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAt                                                      >  2:3872725/1‑150 (MQ=255)

                                                                                           aTAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                                     >  2:4896294/1‑150 (MQ=255)

                                                                                           aTAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtc                                                     >  2:3405317/1‑150 (MQ=255)

                                                                                            tAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAcc                                                                                                                     <  2:4114696/85‑1 (MQ=255)

                                                                                             aGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGAtctc                                                   <  1:3872725/150‑1 (MQ=255)

                                                                                               aaCATCGCTTCCAGCGACAGCCCTTGGGCTGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTccc                                                 <  1:4690069/150‑1 (MQ=255)

                                                                                                aCATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACGTCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                                <  1:342703/150‑1 (MQ=255)

                                                                                                aCATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCt                                                >  2:1897280/1‑150 (MQ=255)

                                                                                                   tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                             >  1:932332/1‑150 (MQ=255)

                                                                                                   tCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTa                                             >  2:3964862/1‑150 (MQ=255)

                                                                                                    cGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑c                                           <  1:3139231/150‑1 (MQ=255)

                                                                                                       ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                         >  1:2486730/1‑118 (MQ=255)

                                                                                                       ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCa                                                                         <  2:2486730/118‑1 (MQ=255)

                                                                                                       ttCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGaa                                        >  2:4548939/1‑150 (MQ=255)

                                                                                                          cAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTc                                     <  1:4087856/150‑1 (MQ=255)

                                                                                                          cAGCGACAGCCCGTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTc                                     <  2:4447473/150‑1 (MQ=255)

                                                                                                           aGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCg                                    >  1:1688348/1‑150 (MQ=255)

                                                                                                              gACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATa                                 <  1:3006766/150‑1 (MQ=255)

                                                                                                                cAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACa                               <  1:2897780/150‑1 (MQ=255)

                                                                                                                 aGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAg                              <  2:4726949/150‑1 (MQ=255)

                                                                                                                  gCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTATCGAATTCG‑TACAgg                             <  2:1649919/150‑1 (MQ=255)

                                                                                                                   cccTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGATGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTATGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGa                            >  1:2372011/1‑150 (MQ=255)

                                                                                                                     cTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGAtt                          >  1:3756876/1‑150 (MQ=255)

                                                                                                                          gCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCAc                                                                                                                      <  1:3098973/54‑1 (MQ=255)

                                                                                                                            aGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAg                   <  2:4060262/150‑1 (MQ=255)

                                                                                                                             gCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAgg                  <  2:2065121/150‑1 (MQ=255)

                                                                                                                                tCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTg               >  1:4718472/1‑150 (MQ=255)

                                                                                                                                tCGATGCACTACAGCGCATCGAGGCTGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGAGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTg               >  2:1322243/1‑150 (MQ=255)

                                                                                                                                  gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc             <  1:4252146/150‑1 (MQ=255)

                                                                                                                                  gATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGAc             >  2:3951882/1‑150 (MQ=255)

                                                                                                                                       gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                                    <  1:3188714/91‑1 (MQ=255)

                                                                                                                                       gcTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCAt                                                                    >  2:3188714/1‑91 (MQ=255)

                                                                                                                                            aGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCATTg   <  1:2618540/150‑1 (MQ=255)

                                                                                                                                             gTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCATTgg  >  2:3463200/1‑150 (MQ=255)

CGACCAACGAATTCAGCCCGATGATCAGCTTGCCTCCGGGCTTCAGGTAGCTCGGCACCCGTTCGAAAAACCTGCGCAAGGTCCGCTCGCCATAGAACATCGCTTCCAGCGACAGCCCTTGGGCAGCCTCGATGCGCTGTAGTGCATCGAGGAAGCCATCCGAGGGCGTCTGCCACCCTGGCGGATTGAATAGGATCACATCGTATCCGTCCTCTGCCGCAGGCATTGCGTCGAACAGATCTCCCTGTA‑CGAATTCGATACAGGATTCGCCCAGGTTGACCAGCTCATTGG  >  NC_002947/4307435‑4307725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`