BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,339	T→A	7.3%	Q15L (CAG→CTG)	trmE ←	GTPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,339	0	T	A	7.3%	557.5 / 25.5	179	Q15L (CAG→CTG)	trmE	GTPase
Reads supporting (aligned to +/- strand): ref base T (79/85); new base A (6/7); total (86/93)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

Only 100 of 181 total aligned reads displayed.

AATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTT  >  NC_002947/6191‑6488

aaTCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTg                                                                                                                                                      <  2:4027553/150‑1 (MQ=255)

  tCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTggg                                                                                                                                                    >  1:4121210/1‑150 (MQ=255)

     cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                                 >  2:2404457/1‑150 (MQ=255)

     cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                                 >  1:4613212/1‑150 (MQ=255)

     cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                                 >  2:401114/1‑150 (MQ=255)

     cTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCg                                                                                                                                                 >  1:3086456/1‑150 (MQ=255)

      ttCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATTGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGt                                                                                                                                                >  1:4991800/1‑150 (MQ=255)

        cGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTgg                                                                                                                                              <  2:1065794/150‑1 (MQ=255)

         gTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGc                                                                                                                                             >  2:3478423/1‑150 (MQ=255)

          tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCg                                                                                                                                            >  1:2204132/1‑150 (MQ=255)

          tCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCg                                                                                                                                            >  1:3346978/1‑150 (MQ=255)

             aGCACCAGCCCTTCAGCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACACCCTGACAATTCCCACACCGCCCCGGCCCGGGGCCGTGGCGATg                                                                                                                                         >  2:3346301/1‑150 (MQ=255)

                aCCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCGGTgtgg                                                                                                                                      <  1:4508961/150‑4 (MQ=255)

                  cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcggc                                                                                                                                    <  2:3844639/150‑1 (MQ=255)

                  cAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATggcggc                                                                                                                                    >  2:4477222/1‑150 (MQ=255)

                   aGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGgcggcg                                                                                                                                   >  1:2615665/1‑150 (MQ=255)

                    gCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGa                                                                                                                                  <  2:2615665/150‑1 (MQ=255)

                     cccTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGAt                                                                                                                                 <  1:3832413/150‑1 (MQ=255)

                        gtcctcgtcgCGGACTGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGt                                                                                                                              <  2:3024360/146‑1 (MQ=255)

                        cgatctgtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATggn                                                                                                                              <  1:681518/144‑2 (MQ=255)

                              gtcgCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACg                                                                                                                        >  2:5333737/1‑150 (MQ=255)

                               tcgCGGAATGCACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGAACAACAGGCCGTCCTAGGCAGCCAGACGGCCCGACAACCTGACAAATCCCACACCGCCCCGGCCCTGGGACGTGGCGATGGCGGCGAAGGttagatcg                                                                                                                       >  2:681518/1‑144 (MQ=255)

                                cgcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGca                                                                                                                      <  1:1936370/150‑1 (MQ=255)

                                 gcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcac                                                                                                                     >  2:4266020/1‑150 (MQ=255)

                                 gcgGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcac                                                                                                                     <  2:3562020/150‑1 (MQ=255)

                                   ggAATGGACCGTCATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGcaccg                                                                                                                   >  2:5142704/1‑148 (MQ=255)

                                     aaTGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAgtg                                                                                                                 >  2:178936/1‑150 (MQ=255)

                                       tGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGtt                                                                                                               >  1:942769/1‑150 (MQ=255)

                                          aCCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                            <  1:4807957/150‑1 (MQ=255)

                                            cGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGc                                                                                                          >  1:2086298/1‑150 (MQ=255)

                                              tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                        <  2:4210631/150‑1 (MQ=255)

                                              tAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTc                                                                                                        <  2:2142700/150‑1 (MQ=255)

                                               aaTGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCACGGCCCAGGGCCGGGGCgg                                                                                                                                           <  1:4501447/114‑2 (MQ=255)

                                                aTGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGa                                                                                                      >  1:4704735/1‑150 (MQ=255)

                                                 tGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaa                                                                                                     <  2:1622556/150‑1 (MQ=255)

                                                 tGGGCAGGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaa                                                                                                     <  1:5244722/150‑1 (MQ=255)

                                                  gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                    <  1:1481201/150‑1 (MQ=255)

                                                  gggCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGaaa                                                                                                    <  1:1088224/150‑1 (MQ=255)

                                                      aTGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCGGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCct                                                                                                <  2:3764394/150‑1 (MQ=255)

                                                       tGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGcccc                                                                                               <  2:1363792/150‑3 (MQ=255)

                                                         ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGTACAACCTGACAATTCCCACACCGCCCCGGCACTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCTCAGTGTTCATGCTCGAAAGCCTCTa                                                                                             <  1:2113063/150‑1 (MQ=255)

                                                         ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTa                                                                                             <  1:4292402/150‑1 (MQ=255)

                                                         ccGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTa                                                                                             <  1:1569631/150‑1 (MQ=255)

                                                                gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                            >  1:16892/1‑128 (MQ=255)

                                                                gTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAt                                                                                                            <  2:16892/128‑1 (MQ=255)

                                                                 tCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACaaaa                                                                                     <  2:4121210/150‑1 (MQ=255)

                                                                   aGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAAc                                                                                   >  1:789941/1‑150 (MQ=255)

                                                                     ggTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGa                                                                                 >  1:993971/1‑150 (MQ=255)

                                                                       tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                               <  2:5046070/150‑1 (MQ=255)

                                                                       tGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                               <  1:1951122/150‑1 (MQ=255)

                                                                           gCCGGTGATCAACTGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCACTGGGCCGTGGAGATGGCGGCTATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATa                                                                           <  2:300333/150‑1 (MQ=255)

                                                                             cGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGc                                                                         >  2:1940012/1‑150 (MQ=255)

                                                                                tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaa                                                                      >  1:5207882/1‑150 (MQ=255)

                                                                                tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaa                                                                      >  1:888016/1‑150 (MQ=255)

                                                                                tGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCaaa                                                                      >  1:1585606/1‑150 (MQ=255)

                                                                                   tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                               >  2:3104596/1‑138 (MQ=255)

                                                                                   tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACa                                                                               <  1:3104596/138‑1 (MQ=255)

                                                                                   tCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAAc                                                                   >  1:3686521/1‑150 (MQ=255)

                                                                                    cAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACg                                                                  >  2:3605488/1‑150 (MQ=255)

                                                                                     aaCAGGCCGGCCTTGGCAGCTAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGc                                                                 <  2:4516019/150‑1 (MQ=255)

                                                                                            cggccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTa                                                          >  1:1304005/1‑150 (MQ=255)

                                                                                               ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAggg                                                       <  2:1618262/150‑1 (MQ=255)

                                                                                               ccTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAggg                                                       <  2:5173875/150‑1 (MQ=255)

                                                                                                      aGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGAt                                                                            <  2:839641/122‑1 (MQ=255)

                                                                                                       gCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttt                                               <  2:4671707/150‑1 (MQ=255)

                                                                                                        ccAGCGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCGGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttttt                                              <  1:620321/150‑1 (MQ=255)

                                                                                                        ccAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGttttt                                              <  2:2770303/150‑1 (MQ=255)

                                                                                                         cAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTGTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGtttttt                                             <  1:3606097/150‑1 (MQ=255)

                                                                                                          agagGGCCTGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTa                                            <  2:4410116/150‑1 (MQ=255)

                                                                                                           gagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTAt                                           <  2:4669793/150‑1 (MQ=255)

                                                                                                           gagGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTGCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACCAACACGACCGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTAt                                           <  2:2547956/150‑1 (MQ=255)

                                                                                                            cgggCCGGACACCCTGACACTGCCCACCCCGCCCCGGCCGTGGGCCGGGGCGCTGGCGGCGATGGTTTCCCGCACCGTGTTCATGCTCGAACGCCTCTACGACAAAAACGACCGATCGCACAAACGCCCCACTAGGGGGCGTTTTTTAtt                                          <  1:5142704/149‑1 (MQ=255)

                                                                                                              ggCCGGACAACCGGACAATTCCCCCCCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCAGGCTCGAACGCCTCTACGCCAAAAACGACCGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTca                                        <  2:2340099/150‑1 (MQ=255)

                                                                                                                ccGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCAACGACAAAAACGACAGATAGCAAAAACTCCCCACTAGGGGGCGTTTTTTATTcaca                                      >  1:3775353/1‑150 (MQ=255)

                                                                                                                ccGGACAACCTGACAATACCCACACCGCCCCGGCCCTGGGCCGGGGCGAGGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTCCGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGATTTTTATTcaca                                      <  2:4628199/150‑1 (MQ=255)

                                                                                                                  ggACAACCTTACACTCCCCACCCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGAACAGTTTTCCTGCTCGAAAGCCTCTACTACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAgg                                    <  1:2302065/150‑1 (MQ=255)

                                                                                                                    aCAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCt                                  >  2:2535062/1‑150 (MQ=255)

                                                                                                                     cAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCCCGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTa                                 <  1:2404897/150‑1 (MQ=255)

                                                                                                                     cAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTa                                 <  2:1555559/150‑1 (MQ=255)

                                                                                                                     cAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTa                                 <  1:2404457/150‑1 (MQ=255)

                                                                                                                        ccTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACGAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCg                              >  2:1470664/1‑150 (MQ=255)

                                                                                                                         cTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGt                             >  2:2471506/1‑150 (MQ=255)

                                                                                                                           gACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTAt                           >  1:139783/1‑150 (MQ=255)

                                                                                                                             cAATTCCCACACCGCCCCGGCCCGGGGCCGTGGCGATGGCGGCTATGGTTTCACGCACAGTTTTAATGCTCGAAAGCCCCAACCACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTa                         <  1:2346604/150‑1 (MQ=255)

                                                                                                                                ttCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGcc                      <  2:2375641/150‑1 (MQ=255)

                                                                                                                                 tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTATATTCACAGGCAAGCGTATTAGccc                     >  1:2555486/1‑150 (MQ=255)

                                                                                                                                 tCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCAAAGTGTTCCTGCTCGAAAGCCTCTACGACAAAAACGACAGAGAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGccc                     <  1:4477222/150‑1 (MQ=255)

                                                                                                                                    cacaCCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTc                  >  2:3527950/1‑150 (MQ=255)

                                                                                                                                     acacCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTATGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCa                 >  2:4815617/1‑150 (MQ=255)

                                                                                                                                       atcGCCACGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAag               >  1:3994221/3‑150 (MQ=255)

                                                                                                                                        tcGCCACGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc              >  1:2882744/2‑150 (MQ=255)

                                                                                                                                        tcGCCACGGCCCAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc              >  2:4539370/2‑150 (MQ=255)

                                                                                                                                        ccGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagc              >  2:485164/1‑150 (MQ=255)

                                                                                                                                         cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagca             >  2:2274036/1‑150 (MQ=255)

                                                                                                                                         cGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagca             >  1:1644734/1‑150 (MQ=255)

                                                                                                                                          gCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcag            <  1:3857596/150‑1 (MQ=255)

                                                                                                                                           ccccGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAagcagc           <  2:5216403/150‑1 (MQ=255)

                                                                                                                                                 ccccGGGCCGCGGCGATGGCGGCGACGCTTTCACGCACAGTGTACATGCTCGAAAGCCCCTACGACAAAAACGACAGATACCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTACCCCGTCAAGCAGCAGCttt     <  1:285335/150‑1 (MQ=255)

                                                                                                                                                 cccAGGGCCGGGGCGGTGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCttt     <  2:3994221/150‑1 (MQ=255)

                                                                                                                                                    tGGGCCGTGGCGAGGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCtttttt  <  2:205872/150‑1 (MQ=255)

AATCCCTTCGTCCAGCACCAGCCCTTCATCGTCGCGGAATGGACCGTAATGGGCATGCCGCGGGGTCAGGGTGCGGCCGGTGATCAACAGGCCGGCCTTGGCAGCCAGAGGGCCGGACAACCTGACAATTCCCACACCGCCCCGGCCCTGGGCCGTGGCGATGGCGGCGATGGTTTCACGCACAGTGTTCATGCTCGAAAGCCTCTACGACAAAAACGACAGATAGCAAAAACGCCCCACTAGGGGGCGTTTTTTATTCACAGGCTAGCGTATTAGCCCGTCAAGCAGCAGCTTTTTT  >  NC_002947/6191‑6488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`