BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,381,108	G→A	5.2%	A114A (GCG→GCA) A66V (GCG→GTG) ‡	PP_5639 → PP_3855 ←	hypothetical protein hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,381,108	0	G	A	5.2%	483.1 / 10.0	153	A114A (GCG→GCA) A66V (GCG→GTG) ‡	PP_5639 PP_3855	hypothetical protein hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (92/53); new base A (4/4); total (96/57)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 157 total aligned reads displayed.

TACATTCAGACCCATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCTGTT  >  NC_002947/4380960‑4381256

tACATTCAGACCCATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGAGTCAATTGTTCTGGCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATAGCAAGCTTCAGCCGGCAGATGCCTCTGGGTCCGgcgc                                                                                                                                                     >  2:4698353/1‑150 (MQ=255)

   aTTCAGACCCATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAgg                                                                                                                                                  >  1:1527559/1‑150 (MQ=255)

   aTTCAGACCCATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAgg                                                                                                                                                  >  2:5015595/1‑150 (MQ=255)

    ttCAGACCCATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGc                                                                                                                                                 >  1:1447520/1‑150 (MQ=255)

           ccATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCg                                                                                                                                          >  2:1791949/1‑150 (MQ=255)

             aTACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGa                                                                                                                                        <  1:4350467/150‑1 (MQ=255)

              tACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAg                                                                                                                                       >  2:2839213/1‑150 (MQ=255)

                 gagaTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCACTATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCt                                                                                                                                    >  2:1208950/1‑150 (MQ=255)

                 gagaTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCt                                                                                                                                    >  2:3344826/1‑150 (MQ=255)

                  agaTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACTCAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCtt                                                                                                                                   <  1:606818/150‑1 (MQ=255)

                  agaTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCtt                                                                                                                                   >  2:195567/1‑150 (MQ=255)

                  agaTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCtt                                                                                                                                   >  2:195675/1‑150 (MQ=255)

                   gaTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGCCtga                                                                                                                                  <  1:1892964/150‑3 (MQ=255)

                    aTTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGCCtgag                                                                                                                                 <  1:4612913/150‑4 (MQ=255)

                      tCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATCTGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCa                                                                                                                               >  1:5376525/1‑150 (MQ=255)

                         gATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATg                                                                                                                            >  1:2107682/1‑150 (MQ=255)

                            cGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGAGGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATc                                                                                                                         <  2:1909697/150‑1 (MQ=255)

                             gACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGCCtga                                                                                                                                  >  1:350138/1‑138 (MQ=255)

                             gACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCAGAGCCTGCGCCGGAGCCtga                                                                                                                                  <  2:350138/140‑3 (MQ=255)

                                            ggCGTTGCTGACACAATTGTTCGGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGt                                                                                                         <  1:3647525/150‑1 (MQ=255)

                                              cGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTaa                                                                                                       <  1:4701335/150‑1 (MQ=255)

                                               gTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAAt                                                                                                      >  2:5382174/1‑150 (MQ=255)

                                                  gCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCt                                                                                                   >  1:2215466/1‑150 (MQ=255)

                                                  gCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCt                                                                                                   <  2:5349387/150‑1 (MQ=255)

                                                   cTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCtt                                                                                                  <  1:2508210/150‑1 (MQ=255)

                                                    tGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTc                                                                                                 >  1:3549669/1‑150 (MQ=255)

                                                    tGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTc                                                                                                 >  2:1988825/1‑150 (MQ=255)

                                                     gACACAATTGTTCTGCCCTACACCTGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCa                                                                                                <  1:3122256/150‑1 (MQ=255)

                                                     gACACAATTGTTCTGCCCTACACCAGCTATCCGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCa                                                                                                <  1:412714/150‑1 (MQ=255)

                                                     gACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCa                                                                                                >  1:3791628/1‑150 (MQ=255)

                                                        acaATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCa                                                                                                                                                    <  1:687320/95‑1 (MQ=255)

                                                          aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTAcc                                                                                           >  2:2734868/1‑150 (MQ=255)

                                                          aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTAcc                                                                                           >  2:2734483/1‑150 (MQ=255)

                                                          aaTTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTAcc                                                                                           >  1:1154886/1‑150 (MQ=255)

                                                                  tGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGt                                                                                   >  1:2136953/1‑150 (MQ=255)

                                                                     ccTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGAAGGCTCCGGCAGAGc                                                                                                                                      <  1:4813796/96‑1 (MQ=255)

                                                                      cTACACGTGCTATCAGCAATACTAGCTAGGCAATATGGTGATACCTAGCTTCAGCCCGCAGATGCCTCAGGCTCGGGCGCTGGCTCTGCGGGAGCCTTCGCAATGATCGACACCTTTGCGCTGTAATCGTTCAGTACCTGGTCGGTTAAt                                                                               <  2:2702224/150‑1 (MQ=255)

                                                                      cTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAAt                                                                               <  2:2702674/150‑1 (MQ=255)

                                                                        acacCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCaa                                                                                                                              >  1:3796806/1‑101 (MQ=255)

                                                                        acacCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCaa                                                                                                                              <  2:3796806/101‑1 (MQ=255)

                                                                        acacCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCaa                                                                                                                              >  1:2652938/1‑101 (MQ=255)

                                                                        acacCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCtt                                                                                                  <  1:216703/129‑1 (MQ=255)

                                                                            cAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgc                                                                         >  1:809657/1‑150 (MQ=255)

                                                                            cAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgc                                                                         >  1:4775543/1‑150 (MQ=255)

                                                                            cAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgc                                                                         >  1:4030463/1‑150 (MQ=255)

                                                                            cAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgc                                                                         >  1:3391004/1‑150 (MQ=255)

                                                                             aGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTgcg                                                                        >  1:4239509/1‑150 (MQ=255)

                                                                                  tCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTc                                                                   >  1:1934303/1‑150 (MQ=255)

                                                                                    aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  1:4621428/1‑150 (MQ=255)

                                                                                    aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  1:1881279/1‑150 (MQ=255)

                                                                                    aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  1:3548397/1‑150 (MQ=255)

                                                                                    aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  2:1438198/1‑150 (MQ=255)

                                                                                    aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  2:2991121/1‑150 (MQ=255)

                                                                                    aGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  2:4185120/1‑150 (MQ=255)

                                                                                     gCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCggg                                                                >  1:1445199/1‑150 (MQ=255)

                                                                                     gCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCggg                                                                >  1:101225/1‑150 (MQ=255)

                                                                                     gCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCggg                                                                >  2:1273259/1‑150 (MQ=255)

                                                                                       aaTACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGaa                                                              <  2:576194/150‑1 (MQ=255)

                                                                                         tACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACt                                                            <  2:521379/150‑1 (MQ=255)

                                                                                         tACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACt                                                            >  2:35151/1‑150 (MQ=255)

                                                                                            aaGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGAc                                                         >  1:3707034/1‑150 (MQ=255)

                                                                                             aGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACt                                                        >  2:4625646/1‑150 (MQ=255)

                                                                                              gCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTc                                                       <  2:1509658/150‑1 (MQ=255)

                                                                                              gCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGGCGGGAACTGACTc                                                       <  2:3862766/150‑1 (MQ=255)

                                                                                              gCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCATTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGAATc                                                       >  1:1874298/1‑150 (MQ=255)

                                                                                                tAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAg                                                     >  2:2609621/1‑150 (MQ=255)

                                                                                                  ggCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGa                                                   >  2:219110/1‑150 (MQ=255)

                                                                                                    cAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATc                                                 >  1:1538938/1‑150 (MQ=255)

                                                                                                         tGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATcgcgcg                                            <  1:2541788/150‑1 (MQ=255)

                                                                                                            ttaTACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 <  2:1689682/124‑1 (MQ=255)

                                                                                                            ttaTACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCgg                                                                 >  1:1689682/3‑126 (MQ=255)

                                                                                                            tGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGc                                         <  2:2928658/150‑1 (MQ=255)

                                                                                                             gATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCa                                        >  2:4894380/1‑150 (MQ=255)

                                                                                                                aCCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACgg                                     <  2:3386375/150‑1 (MQ=255)

                                                                                                                  cAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGACCTGACTCAGGATCGCGCGGGCACGGGt                                   >  1:4315172/1‑150 (MQ=255)

                                                                                                                  cAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGt                                   >  1:4118986/1‑150 (MQ=255)

                                                                                                                  cAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGt                                   >  2:5013579/1‑150 (MQ=255)

                                                                                                                  cAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGt                                   >  2:5335175/1‑150 (MQ=255)

                                                                                                                   aaGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTa                                  >  1:1168285/1‑150 (MQ=255)

                                                                                                                       ttCAGCCCGCAGATGCCTCAGGCTCCGGCGTAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAg                              >  2:3746521/1‑150 (MQ=255)

                                                                                                                       ttCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAg                              >  1:3979541/1‑150 (MQ=255)

                                                                                                                           gCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGcccc                          <  1:5154133/150‑1 (MQ=255)

                                                                                                                           gCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGcccc                          >  1:2764467/1‑150 (MQ=255)

                                                                                                                              cGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCcctc                       >  2:3356359/1‑150 (MQ=255)

                                                                                                                                   aTGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCtgtg                  <  1:1043784/150‑1 (MQ=255)

                                                                                                                                       cgaaGGCTCCGGCAGAGCCTGCGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCGGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagc              >  1:271211/4‑150 (MQ=255)

                                                                                                                                       cTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagc              <  1:1791949/150‑1 (MQ=255)

                                                                                                                                        tCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGt                                                                                   >  2:1973601/1‑80 (MQ=255)

                                                                                                                                        tCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGt                                                                                   <  1:1973601/80‑1 (MQ=255)

                                                                                                                                        tCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcg             >  2:1226556/1‑150 (MQ=255)

                                                                                                                                         cAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcgg            >  2:3458991/1‑150 (MQ=255)

                                                                                                                                           ggCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGACCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcggag          <  2:2224963/150‑1 (MQ=255)

                                                                                                                                            gCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcggagc         >  2:4079885/1‑150 (MQ=255)

                                                                                                                                            gCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcggagc         <  1:5015595/150‑1 (MQ=255)

                                                                                                                                            gCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcggagc         >  1:4904603/1‑150 (MQ=255)

                                                                                                                                            gCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcggagc         <  1:3231213/150‑1 (MQ=255)

                                                                                                                                            gCTCCGGCAGAGCCTGCGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTggagcggagc         >  1:3089123/1‑150 (MQ=255)

                                                                                                                                                 ggCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCtg    <  2:1527559/150‑1 (MQ=255)

                                                                                                                                                   cgcAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCtgtt  <  2:1447520/150‑1 (MQ=255)

                                                                                                                                                   cgcAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCtgtt  <  1:195567/150‑1 (MQ=255)

TACATTCAGACCCATACGAGATTCTGATCGACATGGCTGCGTCTGGCGTTGCTGACACAATTGTTCTGCCCTACACCAGCTATCAGCAATACAAGCTAGGCAATATGGTGATACCAAGCTTCAGCCCGCAGATGCCTCAGGCTCCGGCGCAGGCTCTGCCGGAGCCTTCGCAATGATCGACACCTTAGCGCTGTAATCCTTCAGTACCTGGTCGGTTAATACCTGCGCAGTCGGGAACTGACTCAGGATCGCGCGGGCACGGGTATCAGCCCCCTCCTGTGGAGCGGAGCGGCTGTT  >  NC_002947/4380960‑4381256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`