BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,465,919	A→T	6.2%	*139C (TGA→TGT)	PP_3956 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,465,919	0	A	T	6.2%	587.0 / 19.1	176	*139C (TGA→TGT)	PP_3956	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (86/78); new base T (5/6); total (91/85)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 179 total aligned reads displayed.

CCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTT  >  NC_002947/4465771‑4466068

  gAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACgg                                                                                                                                                    <  2:4625355/150‑1 (MQ=255)

     gCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGa                                                                                                                                                 <  2:753884/150‑1 (MQ=255)

      cTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGAc                                                                                                                                                >  1:2610270/1‑150 (MQ=255)

       tGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACa                                                                                                                                               >  2:3454034/1‑150 (MQ=255)

          tGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAggg                                                                                                                                            <  1:4805995/150‑1 (MQ=255)

             cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAg                                                                                                                                         <  1:2653669/150‑1 (MQ=255)

             cTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAg                                                                                                                                         <  1:2653474/150‑1 (MQ=255)

              tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAgg                                                                                                                                        >  2:529248/1‑150 (MQ=255)

              tACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAgg                                                                                                                                        >  1:2168025/1‑150 (MQ=255)

               aCCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGa                                                                                                                                       <  1:2369648/150‑1 (MQ=255)

               aCCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGa                                                                                                                                       <  1:3423259/150‑1 (MQ=255)

               aCCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGa                                                                                                                                       <  1:2369732/150‑1 (MQ=255)

                ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAc                                                                                                                                      >  2:857896/1‑150 (MQ=255)

                ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAc                                                                                                                                      >  2:4868387/1‑150 (MQ=255)

                ccAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAc                                                                                                                                      <  2:4789415/150‑1 (MQ=255)

                 cAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGTCCTGCCCTGTCGCCGTCAGGGCAgggcg                                                                                                                                     <  1:2494320/150‑4 (MQ=255)

                  aGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGAcgg                                                                                                                                    <  2:5285042/150‑3 (MQ=255)

                    gTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGaa                                                                                                                                  <  2:2174382/150‑1 (MQ=255)

                     tCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAAt                                                                                                                                 <  1:3298043/150‑1 (MQ=255)

                        gCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCa                                                                                                                              <  2:850855/150‑1 (MQ=255)

                        gCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCa                                                                                                                              <  1:1591394/150‑1 (MQ=255)

                         cACGACAGACGGGTGCCAGCCACTGTCCTATTTGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCGCTGCCCTGACGGCGACAGGGCAGGACCGAATCCAc                                                                                                                             <  2:4083974/150‑1 (MQ=255)

                            gACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTc                                                                                                                          >  1:307388/1‑150 (MQ=255)

                            gACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTc                                                                                                                          >  1:4303398/1‑150 (MQ=255)

                                 aCGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGccc                                                                                                                     >  2:4787308/1‑150 (MQ=255)

                                 aCGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTCACGGCGACAGGGCAGGACCGAATCCACTTCAGccc                                                                                                                     >  1:2838687/1‑150 (MQ=255)

                                     gTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCccacca                                                                                                                 <  1:3636720/150‑1 (MQ=255)

                                        ccagccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGt                                                                                                              >  2:2025798/1‑150 (MQ=255)

                                        ccagccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGt                                                                                                              <  1:4967374/150‑1 (MQ=255)

                                          agccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGc                                                                                                            <  1:3391823/150‑1 (MQ=255)

                                            ccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCGGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCaa                                                                                                          <  2:692339/150‑1 (MQ=255)

                                            ccaCTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCaa                                                                                                          <  2:4912232/150‑1 (MQ=255)

                                                tGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGTGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                      >  1:5015829/1‑150 (MQ=255)

                                                tGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                      <  2:5380747/150‑1 (MQ=255)

                                                tGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                      <  2:1094244/150‑1 (MQ=255)

                                                tGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                      <  1:3777848/150‑1 (MQ=255)

                                                tGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCCGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTaa                                                                                                      >  1:2036068/1‑150 (MQ=255)

                                                 gTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAAt                                                                                                     <  1:1121875/150‑1 (MQ=255)

                                                  tCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATg                                                                                                    >  1:3855313/1‑150 (MQ=255)

                                                   ccTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGc                                                                                                   <  1:971400/150‑1 (MQ=255)

                                                     tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 <  2:2203408/150‑1 (MQ=255)

                                                     tATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAg                                                                                                 >  1:5194539/1‑150 (MQ=255)

                                                          gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAggg                                                                                                                                            <  2:3239639/102‑1 (MQ=255)

                                                          gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAggg                                                                                                                                            >  1:3239639/1‑102 (MQ=255)

                                                          gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGc                                                                                            >  2:4244453/1‑150 (MQ=255)

                                                          gTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGc                                                                                            >  2:5285012/1‑150 (MQ=255)

                                                           ttAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCg                                                                                           <  2:4009835/150‑1 (MQ=255)

                                                           ttAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCg                                                                                           <  2:3293021/150‑1 (MQ=255)

                                                           ttAGGACCGCCGAGCGAACGCATTGATGCAGTAGAATTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTTGCg                                                                                           <  2:280340/150‑1 (MQ=255)

                                                              ggACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTc                                                                                        >  1:5264987/1‑150 (MQ=255)

                                                              ggACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTc                                                                                        <  1:476486/150‑1 (MQ=255)

                                                               gACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGTCGCCGTCAGGGCAgg                                                                                                                                        >  2:273995/1‑101 (MQ=255)

                                                               gACCGCCGAGCGAACGCATTGCAGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTGTGGACCGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTACTGCAGGTGGCgggcg                                                                                       >  2:2995999/1‑146 (MQ=255)

                                                                   gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTgg                                                                                             <  1:2713615/140‑1 (MQ=255)

                                                                   gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTgg                                                                                             >  2:2713615/1‑140 (MQ=255)

                                                                   gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                   <  2:5015587/150‑1 (MQ=255)

                                                                   gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                   >  1:2900663/1‑150 (MQ=255)

                                                                   gccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACt                                                                                   >  1:1202698/1‑150 (MQ=255)

                                                                    ccgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTg                                                                                  <  1:1657600/150‑1 (MQ=255)

                                                                     cgAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGa                                                                                 >  2:1939452/1‑150 (MQ=255)

                                                                       aGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATc                                                                               <  1:4695295/150‑1 (MQ=255)

                                                                        gCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATcc                                                                              <  1:4244453/150‑1 (MQ=255)

                                                                          gAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAg                                                                            <  1:555233/150‑1 (MQ=255)

                                                                          gAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAg                                                                            >  1:1022467/1‑150 (MQ=255)

                                                                             cGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGAt                                                                         <  1:784450/150‑1 (MQ=255)

                                                                             cGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTGGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGAt                                                                         <  1:784468/150‑1 (MQ=255)

                                                                                aTTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGTCGCCGTCAGGGCAg                                                                                                                                         >  1:1065779/1‑83 (MQ=39)

                                                                                aTTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAAt                                                                                                                                 >  2:2638720/1‑91 (MQ=255)

                                                                                aTTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTc                                                                      >  1:3682709/1‑150 (MQ=255)

                                                                                   gctgcAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGcc                                                                   >  1:3482435/1‑150 (MQ=255)

                                                                                     tgcAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCaa                                                                 >  2:4101533/1‑150 (MQ=255)

                                                                                       cAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATc                                                               >  2:5423970/1‑150 (MQ=255)

                                                                                       cAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATc                                                               <  1:4783146/150‑1 (MQ=255)

                                                                                        aGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAg                                                                            >  1:2136479/1‑136 (MQ=255)

                                                                                          tAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATcaca                                                            <  1:857896/150‑1 (MQ=255)

                                                                                               ttCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTc                                                       >  1:2671521/1‑150 (MQ=255)

                                                                                               ttCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTc                                                       >  1:66484/1‑150 (MQ=255)

                                                                                               ttCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTc                                                       >  1:4590071/1‑150 (MQ=255)

                                                                                                    ggCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGc                                                  <  1:2473136/150‑1 (MQ=255)

                                                                                                      ccTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTc                                                <  1:3712270/150‑1 (MQ=255)

                                                                                                              aaTCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCat                                        <  1:2758699/150‑1 (MQ=255)

                                                                                                                tCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCatga                                      <  2:4551901/150‑1 (MQ=255)

                                                                                                                     cTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCg                                 >  2:3293031/1‑150 (MQ=255)

                                                                                                                          gACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTc                            >  1:3292220/1‑150 (MQ=255)

                                                                                                                            cagcagGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCt                          >  2:1738002/1‑150 (MQ=255)

                                                                                                                                agGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcc                      >  2:1178305/1‑150 (MQ=255)

                                                                                                                                agGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGcc                      >  1:406146/1‑150 (MQ=255)

                                                                                                                                     aaCCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGCGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCtt                 <  2:2439380/150‑1 (MQ=255)

                                                                                                                                      aCCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtc                >  2:1068658/1‑150 (MQ=255)

                                                                                                                                       ccGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTtct               <  2:2671521/150‑1 (MQ=255)

                                                                                                                                         gtcctgccctgTCGCCGTCAGGGCAGGGCCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                     <  1:830016/76‑1 (MQ=37)

                                                                                                                                         gtcctgccctgTCGCCGTCAGGGCAGGGCCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTa                                                                                     >  2:830016/3‑78 (MQ=37)

                                                                                                                                          ccctgccctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGc            >  2:3796388/1‑150 (MQ=255)

                                                                                                                                             tgccctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGa         <  2:3603833/150‑1 (MQ=255)

                                                                                                                                              gccctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGAt        <  2:3901105/150‑1 (MQ=255)

                                                                                                                                                 ctgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCa     >  2:2430676/1‑150 (MQ=255)

                                                                                                                                                  tgACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCAc    <  2:2610270/150‑1 (MQ=255)

                                                                                                                                                    aCGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACtt  >  2:1632849/1‑150 (MQ=255)

                                                                                                                                                    aCGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACtt  >  2:3776860/1‑150 (MQ=255)

                                                                                                                                                    aCGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACtt  >  2:3720273/1‑150 (MQ=255)

CCGAGGCTGGTGACTACCAGGTCTGCACGACAGACGGGTGCCAGCCACTGTCCTATTGGTTAGGACCGCCGAGCGAACGCATTGCTGCAGTAGACTTCTGGGCCTGTGAAAATCCGCCTTTGGACAGCAGGCGAACCGCCCTGCCCTGACGGCGACAGGGCAGGACCGAATCCACTTCAGCCCACCACGTGCAAGTAATGCAGGTGGCGTTCGTACTGATCCAGGATGTCGCCAATCACATCGTCACGGCTCCACCCCATGATGTCGTAGTCCTGGCCCCCTTCTCGCAGATGCACTT  >  NC_002947/4465771‑4466068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`