Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,582,566 T→A 5.7% Q943L (CAG→CTG) ‡ treSB ← bifunctional trehalose synthase B/maltokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,582,5660TA 5.7% 510.1 / 10.9 151Q943L (CAG→CTG) ‡treSBbifunctional trehalose synthase B/maltokinase
Reads supporting (aligned to +/- strand):  ref base T (63/79);  new base A (5/4);  total (68/83)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

CCTTGTAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACAC  >  NC_002947/4582417‑4582715
                                                                                                                                                     |                                                                                                                                                     
ccTTGTAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCt                                                                                                                                                       >  2:1662360/1‑150 (MQ=255)
 cTTGTAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtg                                                                                                                                                      >  1:2561641/1‑150 (MQ=255)
       aGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCtgcttgct                                                                                                                                                >  2:2021630/1‑150 (MQ=255)
         ggCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCa                                                                                                                                              <  2:1232678/150‑1 (MQ=255)
           cTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAgg                                                                                                                                            <  2:1338219/150‑1 (MQ=255)
           cTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTAAGCCTGCTTGCTCAgg                                                                                                                                            <  2:3957033/150‑1 (MQ=255)
            tgtgTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAggg                                                                                                                                           >  1:480257/1‑150 (MQ=255)
            tgtgTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAggg                                                                                                                                           >  1:1422860/1‑150 (MQ=255)
            tgtgTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAggg                                                                                                                                           <  2:663018/150‑1 (MQ=255)
            tgtgTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAggg                                                                                                                                           <  2:2933917/150‑1 (MQ=255)
                ttttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTc                                                                                                                                       <  2:1902171/150‑1 (MQ=255)
                ttttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTc                                                                                                                                       <  1:4055206/150‑1 (MQ=255)
                ttttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTc                                                                                                                                       <  1:3987172/150‑1 (MQ=255)
                  ttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGa                                                                                                                                     >  1:3156811/1‑150 (MQ=255)
                  ttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGa                                                                                                                                     <  1:1163256/150‑1 (MQ=255)
                  ttGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGACACCCTGAGCAAGCAGGCTCAGGgtggcct                                                                                                                                     <  2:5313207/150‑5 (MQ=255)
                    gCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGAtg                                                                                                                                   >  1:3194715/1‑150 (MQ=255)
                     cccGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGgtggcctgct                                                                                                                                  >  1:2942132/1‑143 (MQ=255)
                         gcgcTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTg                                                                                                                              >  2:5260740/1‑150 (MQ=255)
                          cgcTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTgg                                                                                                                             <  1:2163423/150‑1 (MQ=255)
                           gcTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTggg                                                                                                                            >  2:2703492/1‑150 (MQ=255)
                            ctctTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGc                                                                                                                           <  2:4711822/150‑1 (MQ=255)
                            ctctTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGc                                                                                                                           <  2:4566312/150‑1 (MQ=255)
                             tctTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTgg                                                                                                                             >  2:3696455/1‑147 (MQ=255)
                             tctTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTgg                                                                                                                             <  1:3696455/147‑1 (MQ=255)
                               ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAgg                                                                                                                                            <  2:2922826/130‑1 (MQ=255)
                               ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAgg                                                                                                                                            >  1:2922826/1‑130 (MQ=255)
                               ttCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAg                                                                                                                        <  2:1850958/150‑1 (MQ=255)
                                  aaGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGcc                                                                                                                     <  2:1860690/150‑1 (MQ=255)
                                  aaGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGcc                                                                                                                     >  1:4340034/1‑150 (MQ=255)
                                  aaGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGcc                                                                                                                     >  2:2937750/1‑150 (MQ=255)
                                      ggCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCa                                                                                                                 <  2:3847767/150‑1 (MQ=255)
                                      ggCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCa                                                                                                                 <  2:3847803/150‑1 (MQ=255)
                                       gCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCAc                                                                                                                >  2:3028680/1‑150 (MQ=255)
                                           ggcgggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTg                                                                                                            <  1:618871/150‑1 (MQ=255)
                                             cgggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGtt                                                                                                          <  2:4644203/150‑1 (MQ=255)
                                               ggTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACAAGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTtgc                                                                                                        >  2:3039459/1‑150 (MQ=255)
                                                      cTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgt                                                                                                 >  1:1867051/1‑150 (MQ=255)
                                                      cTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGAATAACCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgt                                                                                                 >  1:3439036/1‑150 (MQ=255)
                                                       ttCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGt                                                                                                           <  1:2162310/139‑1 (MQ=255)
                                                       ttCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGt                                                                                                           >  2:2162310/1‑139 (MQ=255)
                                                       ttCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgtc                                                                                                >  1:796427/1‑150 (MQ=255)
                                                       ttCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGgtc                                                                                                >  2:4928996/1‑150 (MQ=255)
                                                           aaaTCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGtcgtcg                                                                                            >  1:5049533/1‑150 (MQ=255)
                                                            caTCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATCAGCAGGCCACCCTGAGCGTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGa                                                                                           <  2:5450504/149‑1 (MQ=255)
                                                            aaTCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGa                                                                                           >  2:1648076/1‑150 (MQ=255)
                                                                 aTGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAg                                                                                      <  1:5098931/150‑1 (MQ=255)
                                                                    aGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGAc                                                                                   <  1:1122348/150‑1 (MQ=255)
                                                                    aGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGAc                                                                                   <  1:1722683/150‑1 (MQ=255)
                                                                       tAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTgg                                                                                <  2:1457181/150‑1 (MQ=255)
                                                                            ctCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCACGGACTGGCTATc                                                                           >  2:2285883/2‑150 (MQ=255)
                                                                            aTCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATc                                                                           <  1:996192/150‑1 (MQ=255)
                                                                               aCCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCt                                                                        <  1:3584778/150‑1 (MQ=255)
                                                                                  cTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTc                                                                     <  1:2490569/150‑1 (MQ=255)
                                                                                  cTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTc                                                                     <  2:2434955/150‑1 (MQ=255)
                                                                                  cTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCACAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTtg                                                                                                         >  1:293669/1‑114 (MQ=255)
                                                                                     caccacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAg                                                                  >  2:3032402/1‑150 (MQ=255)
                                                                                      accacGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGc                                                                                                                           <  1:1044916/92‑1 (MQ=255)
                                                                                           gAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGTAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTc                                                            >  1:4939944/1‑150 (MQ=255)
                                                                                               aCCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCAAGCAGGCTCAGGgt                                                                                                                                          <  2:2596741/68‑1 (MQ=37)
                                                                                                 cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGAc                                                                                          >  2:1227182/1‑114 (MQ=255)
                                                                                                 cTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATg                                                      <  2:796427/150‑1 (MQ=255)
                                                                                                   ggCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgt                                                                 >  1:515289/1‑137 (MQ=255)
                                                                                                   ggCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAgt                                                                 <  2:515289/137‑1 (MQ=255)
                                                                                                    gCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTg                                                   <  2:5036928/150‑1 (MQ=255)
                                                                                                    gCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTg                                                   >  1:3670808/1‑150 (MQ=255)
                                                                                                     cccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTgg                                                  <  2:2854630/150‑1 (MQ=255)
                                                                                                     cccAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTgg                                                  <  1:4137926/150‑1 (MQ=255)
                                                                                                          gTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCcag                                             >  1:4537637/1‑150 (MQ=255)
                                                                                                              aGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGGGTGTCGCGATGCTGGGCCAGCAgg                                         >  1:943166/1‑150 (MQ=255)
                                                                                                                gTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTc                                       >  1:2950529/1‑150 (MQ=255)
                                                                                                                   gCCGTGTACACTCATAAGCAGGACACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCTCGATGCTGGGCCAGCAGGTCGAg                                    <  2:4277833/150‑1 (MQ=255)
                                                                                                                   gCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAg                                    <  2:4680858/150‑1 (MQ=255)
                                                                                                                    ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAgg                                   <  1:2021630/150‑1 (MQ=255)
                                                                                                                    ccGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAgg                                   <  2:3617111/150‑1 (MQ=255)
                                                                                                                     cGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAggg                                  >  1:1295059/1‑150 (MQ=255)
                                                                                                                     cGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAggg                                  >  1:1295241/1‑150 (MQ=255)
                                                                                                                       tgtACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCg                                <  2:5314063/150‑1 (MQ=255)
                                                                                                                        gtACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGt                               >  2:1382313/1‑150 (MQ=255)
                                                                                                                          acacGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                             <  2:3156819/150‑1 (MQ=255)
                                                                                                                          acacGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTgg                             <  2:3156811/150‑1 (MQ=255)
                                                                                                                           cacGCCTAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGGGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTggg                            >  2:4412657/1‑150 (MQ=255)
                                                                                                                           cacGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTggg                            <  2:2535499/150‑1 (MQ=255)
                                                                                                                           cacGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTggg                            >  2:1336195/1‑150 (MQ=255)
                                                                                                                              gCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCaa                                                                                       <  2:2382051/88‑1 (MQ=255)
                                                                                                                              gCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtca                         <  2:1422860/150‑1 (MQ=255)
                                                                                                                              gCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtca                         <  2:2970749/150‑1 (MQ=255)
                                                                                                                                 tAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagtt                      <  1:962669/150‑1 (MQ=255)
                                                                                                                                   aGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCa                                                                                                    <  1:1306826/70‑1 (MQ=255)
                                                                                                                                    gCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagttcag                   >  1:3427068/1‑150 (MQ=255)
                                                                                                                                     cAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGtcagttcagt                  >  1:3076016/1‑150 (MQ=255)
                                                                                                                                          cACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTTGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTTTAGCTGTACAGTGTGTCGCGATGCTTGGCCAGCAGGTCGTTGGAGTGTGTCAGTTCATTTCTGa             <  2:3439036/150‑1 (MQ=255)
                                                                                                                                           aCCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGAt            >  1:4822604/1‑150 (MQ=255)
                                                                                                                                            cccTGAGCAAGCAGGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCGGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGGGGGTCAGTGCAGTGCTGATc           >  2:3970648/1‑150 (MQ=255)
                                                                                                                                             ccTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGa             <  1:2696829/147‑1 (MQ=255)
                                                                                                                                                 aGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGc      <  1:3554945/150‑1 (MQ=255)
                                                                                                                                                  gCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGca     <  2:3477411/150‑1 (MQ=255)
                                                                                                                                                   ccTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGcac    >  2:3304341/1‑150 (MQ=255)
                                                                                                                                                   ccTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGcac    >  1:1194353/1‑150 (MQ=255)
                                                                                                                                                   ccTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGAGCTGATCTGTGcac    >  1:5115755/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTTGTAAGGGCTGTGTTTTGCCCGGCGCTCTTCAAGGGGCCGGGCGGGTTCACCTTCAAAATCGATGAGGTAGGCATCACCCTGCACCACGAGCACCTGGCCCAGGTGCAGGTCGCCGTGTACACGCATAAGCAGGCCACCCTGAGCCTGCTTGCTCAGGGTGTCGATGTGCTGGGCCAGGCCGTCACGCTGTTGCTGCAGGTCGTCGACCAAGGACTGGCTATCGCTGTCCAGTGTGTCGCGATGCTGGGCCAGCAGGTCGAGGGCGTGGGTCAGTTCAGTGCTGATCTGTGCACAC  >  NC_002947/4582417‑4582715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑