BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	14,925	C→G	9.3%	A532A (GCC→GCG)	gyrB →	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	14,925	0	C	G	9.3%	519.6 / 33.7	162	A532A (GCC→GCG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand): ref base C (77/70); new base G (8/7); total (85/77)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 169 total aligned reads displayed.

GAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGTGCAGCT  >  NC_002947/14776‑15074

gAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGcc                                                                                                                                                       <  1:4282179/150‑1 (MQ=255)

 aaGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                                                                                                                                                      >  1:4881913/1‑150 (MQ=255)

 aaGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGccc                                                                                                                                                      >  1:4161503/1‑150 (MQ=255)

  agagTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCa                                                                                                                                                     <  2:4857000/150‑1 (MQ=255)

    agTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgc                                                                                                                                                   <  2:1296730/150‑1 (MQ=255)

      tACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccg                                                                                                                                                 >  1:2150101/1‑150 (MQ=255)

       acaacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAgccgc                                                                                                                                                >  2:3788120/1‑150 (MQ=255)

         aacaTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGccgccg                                                                                                                                              >  1:2076839/1‑150 (MQ=255)

           tcctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCt                                                                                                                                            >  1:658744/5‑150 (MQ=255)

            cctgACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCtc                                                                                                                                           >  1:2877393/4‑149 (MQ=255)

            aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                                                                                                           >  1:4110836/1‑150 (MQ=255)

            aTCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTg                                                                                                                                           >  1:3657806/1‑150 (MQ=255)

               gACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGACCTGATCGAGCGTGGCCAGATCTATATCGCGCAGCCGCCGCtctct                                                                                                                                        >  2:4825144/1‑146 (MQ=255)

               gACAAGCTGCGCTACCATAAGATCATCATCATGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTat                                                                                                                                        >  2:3955712/1‑149 (MQ=255)

               gACAAACTGCGTTATCACAACATCATCATCATGACCAATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTAc                                                                                                                                        >  2:4301445/1‑150 (MQ=255)

                 cAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACaa                                                                                                                                      <  2:3529576/150‑1 (MQ=255)

                         gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                                                              >  1:1379513/1‑150 (MQ=255)

                         gTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAAc                                                                                                                              <  2:1515227/150‑1 (MQ=255)

                           tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGa                                                                                                                            <  2:1372269/150‑1 (MQ=255)

                           tATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGa                                                                                                                            <  2:1464143/150‑1 (MQ=255)

                            aTCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAg                                                                                                                           >  2:604536/1‑150 (MQ=255)

                               acaacaTCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCa                                                                                                                        >  2:2426652/1‑150 (MQ=255)

                                    atcatcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAg                                                                                                                   >  2:2623106/1‑150 (MQ=255)

                                      catcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGa                                                                                                                 <  1:5036736/150‑1 (MQ=255)

                                       atcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAg                                                                                                                <  2:4119452/150‑1 (MQ=255)

                                        tcatcatGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGTGTCTCGCGGCAAgtcctcgc                                                                                                               >  1:3490498/1‑143 (MQ=255)

                                        tcatcatGACGGATGCTGACGTCGACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGTGTCTCGCGGCAAgtcctcgc                                                                                                               <  2:2113750/150‑8 (MQ=255)

                                        tcatcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGc                                                                                                               >  1:4280325/1‑150 (MQ=255)

                                         catcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCa                                                                                                              <  2:1444741/150‑1 (MQ=255)

                                           tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt                                                                                                            <  2:3011695/150‑1 (MQ=255)

                                           tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt                                                                                                            <  2:4844479/150‑1 (MQ=255)

                                           tcatGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGt                                                                                                            >  1:937065/1‑150 (MQ=255)

                                             atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTAc                                                                                                          >  1:1358059/1‑150 (MQ=255)

                                             atGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTAc                                                                                                          >  1:4556821/1‑150 (MQ=255)

                                                  cGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAATCAGGAGCAGTACATCaa                                                                                                     >  2:2881563/1‑150 (MQ=255)

                                                  cGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCaa                                                                                                     <  2:2150101/150‑1 (MQ=255)

                                                    aTGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAgg                                                                                                   <  1:2792384/150‑1 (MQ=255)

                                                     tGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGa                                                                                                  <  2:5279753/150‑1 (MQ=255)

                                                        tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                                                               >  2:728687/1‑150 (MQ=255)

                                                        tGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGa                                                                                               >  1:1269094/1‑150 (MQ=255)

                                                         gACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGAt                                                                                              <  2:4280325/150‑1 (MQ=255)

                                                           cGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGa                                                                                            <  2:274331/150‑1 (MQ=255)

                                                            gtcgACGGTGCGCATATTCGTACGCTGCTGTTGACCTTCTTCTTCCGTCAGATGCCGGAACTGATCGAGCGTGGCCATATCTATATCGCGCAGCCGCCGCTCTATAAGGt                                                                                                                                   <  2:5000777/107‑1 (MQ=255)

                                                            gTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAg                                                                                           <  2:3448039/150‑1 (MQ=255)

                                                             ttGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAgg                                                                                          <  1:4641355/150‑1 (MQ=255)

                                                              tGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGTCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAg                                                                                                                <  1:1230716/127‑1 (MQ=255)

                                                              tGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTAAATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAg                                                                                                                >  2:1230716/1‑127 (MQ=255)

                                                                  ggTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                                                                     <  2:2511641/150‑1 (MQ=255)

                                                                  ggTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATg                                                                                     <  1:997911/150‑1 (MQ=255)

                                                                     tCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGaa                                                                                  <  2:1279096/150‑1 (MQ=255)

                                                                       gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                                                <  2:1468028/150‑1 (MQ=255)

                                                                       gCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAaga                                                                                <  2:10420/150‑1 (MQ=255)

                                                                          caTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTa                                                                             <  2:526896/150‑1 (MQ=255)

                                                                          caTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTa                                                                             <  1:4422357/150‑1 (MQ=255)

                                                                           aTCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                                                            >  1:411030/1‑150 (MQ=255)

                                                                            tCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACa                                                                           <  1:1842440/150‑1 (MQ=255)

                                                                               gTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGa                                                                        <  2:3942038/150‑1 (MQ=255)

                                                                                tACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAc                                                                       >  1:5211672/1‑150 (MQ=255)

                                                                                 aCGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAcc                                                                      <  1:5435261/150‑1 (MQ=255)

                                                                                 aCGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAcc                                                                      <  1:5435134/150‑1 (MQ=255)

                                                                                      gctgctgACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTc                                                                 >  2:1539795/1‑150 (MQ=255)

                                                                                              ccTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTgg                                                         <  2:1415533/150‑1 (MQ=255)

                                                                                               cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                                                        >  2:2787813/1‑150 (MQ=255)

                                                                                               cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                                                        >  1:3087760/1‑150 (MQ=255)

                                                                                               cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                                                        >  2:1818344/1‑150 (MQ=255)

                                                                                               cttcttcttCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGa                                                        >  1:4504180/1‑150 (MQ=255)

                                                                                                ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                                                       >  2:5163882/1‑150 (MQ=255)

                                                                                                ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                                                       >  1:3029454/1‑150 (MQ=255)

                                                                                                ttcttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGaa                                                       >  1:5322727/1‑150 (MQ=255)

                                                                                                  cttcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGa                                                     >  2:294781/1‑150 (MQ=255)

                                                                                                  ccagctcCGGCAGCTGACGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGa                                                     >  1:3757938/6‑150 (MQ=255)

                                                                                                    tcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGTAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATg                                                   <  1:4597139/150‑1 (MQ=255)

                                                                                                    tcttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATg                                                   >  1:2001745/1‑150 (MQ=255)

                                                                                                     cttcCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGc                                                  >  2:723172/1‑150 (MQ=255)

                                                                                                                  ccGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTg                                     >  1:503156/1‑150 (MQ=255)

                                                                                                                  ccGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTg                                     >  1:4199710/1‑150 (MQ=255)

                                                                                                                   cGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAGCGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTAc                                                                            <  2:4972121/110‑1 (MQ=255)

                                                                                                                   cGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTgg                                    <  1:4043764/150‑1 (MQ=255)

                                                                                                                   cGGAGCTGGTCGAGCGTGGCTAAATCTATATTGCCAAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTgg                                    >  1:4829331/1‑150 (MQ=255)

                                                                                                                     gAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGAc                                  <  1:4757023/150‑1 (MQ=255)

                                                                                                                       gCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGAcc                                                                      <  2:5077461/112‑1 (MQ=255)

                                                                                                                       gCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGa                                <  2:3838945/150‑1 (MQ=255)

                                                                                                                          ggTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATc                             >  2:4698671/1‑150 (MQ=255)

                                                                                                                           gTCGAGCGTGGCTACATCTATATTGCCCAGACGCAGCTGTACAAGGAGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCACTCGGCTCTGGAAGATTACAGCCTGCACCTGGACGAATCg                            >  1:3570111/1‑150 (MQ=255)

                                                                                                                            tCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCgg                           >  2:2195333/1‑150 (MQ=255)

                                                                                                                               aGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGgcgc                        >  1:3788280/1‑150 (MQ=255)

                                                                                                                                 cGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCca                      <  1:1867856/150‑1 (MQ=255)

                                                                                                                                  gTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCcag                     >  2:2588094/1‑150 (MQ=255)

                                                                                                                                    ggCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCcagca                   <  2:503156/150‑1 (MQ=255)

                                                                                                                                      cTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGt                 <  1:4301445/150‑1 (MQ=255)

                                                                                                                                        aCATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGttt               <  1:3907752/150‑1 (MQ=255)

                                                                                                                                            cTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCgg           >  2:5078899/1‑150 (MQ=255)

                                                                                                                                              atatTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCg         >  1:4830135/1‑150 (MQ=255)

                                                                                                                                              atatTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCg         >  1:1139631/1‑150 (MQ=255)

                                                                                                                                               tatTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGt        <  1:1508265/150‑1 (MQ=255)

                                                                                                                                               tatTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGt        >  1:3149887/1‑150 (MQ=255)

                                                                                                                                               tatTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGt        >  1:3150175/1‑150 (MQ=255)

                                                                                                                                                    cccAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGTGCAGc   >  1:238341/1‑150 (MQ=255)

                                                                                                                                                     ccAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGTGCAGCt  <  1:728798/150‑1 (MQ=255)

                                                                                                                                                     ccAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGTGCAGCt  <  1:728687/150‑1 (MQ=255)

GAAGAGTACAACATCGACAAACTGCGTTATCACAACATCATCATCATGACCGATGCTGACGTTGACGGTTCGCACATCCGTACGCTGCTGCTGACCTTCTTCTTCCGTCAGCTGCCGGAGCTGGTCGAGCGTGGCTACATCTATATTGCCCAGCCGCCGCTGTACAAGGTGAAACGAGGCAAGCAGGAGCAGTACATCAAGGACGATGAGGCCATGGAAGAGTACATGACCCAGTCGGCTCTGGAAGATGCCAGCCTGCACCTGGACGAATCGGCGCCAGCAGTTTCCGGCGTGCAGCT  >  NC_002947/14776‑15074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`